FastQCFastQC Report
Thu 26 May 2016
SRR1547780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547780_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences919963
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55200.6000241314052848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30570.3322959727728181No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21890.2379443521098131No Hit
TAACAGGGGATGAGCACGGGATTTACAACTGTGACATCTCAAGATGGTCA18260.1984862434684873No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC9750.10598252321017258No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC9690.10533032306734075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT150.001309707380.000014
TAGCGCG150.001309707380.000011
ATCGTAG150.001309707380.000011
TCGTGAA150.001309707380.000011
CGGGACG304.0592568E-880.000016
GCGGACC150.001309707380.000015
CCGAACG304.0592568E-880.000011
CACGTTG304.0592568E-880.000011
TATCGTG150.001309707380.000011
CGATATC304.0592568E-880.000019
ATACCGG150.001309707380.000012
CTAGACG204.0904655E-580.01
ACCGGGT500.080.04
TTGTGCG404.1836756E-1180.01
ATTACCG550.080.01
GCACGAG204.0904655E-580.01
ACGGGTA404.1836756E-1180.05
GCCGCAC351.2896635E-980.08
TAGGCGA404.1836756E-1180.01
TGGGCGA1650.077.575756
Produced by FastQC (version 0.11.5)