##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547780_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	919963
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78224776431226	31.0	31.0	33.0	30.0	34.0
2	32.21818920978344	33.0	31.0	34.0	30.0	34.0
3	32.337672275950226	34.0	31.0	34.0	30.0	34.0
4	35.92140553478781	37.0	35.0	37.0	35.0	37.0
5	35.85698663968007	37.0	35.0	37.0	35.0	37.0
6	35.91249865483721	37.0	35.0	37.0	35.0	37.0
7	36.23110712061246	37.0	35.0	37.0	35.0	37.0
8	36.20266358538333	37.0	36.0	37.0	35.0	37.0
9	38.10155951924153	39.0	39.0	39.0	37.0	39.0
10-11	37.4973748944251	39.0	37.5	39.0	35.0	39.0
12-13	36.07366491913261	37.0	35.0	39.0	32.5	39.0
14-15	36.965083378353256	38.0	35.0	41.0	32.5	41.0
16-17	37.268812984870046	38.0	35.0	41.0	33.0	41.0
18-19	37.192954499257034	38.0	35.0	41.0	33.0	41.0
20-21	36.920342448555	38.0	35.0	41.0	33.0	41.0
22-23	36.70983887395471	37.5	35.0	41.0	32.5	41.0
24-25	36.621144002530535	37.0	35.0	41.0	32.5	41.0
26-27	36.54998679294711	37.0	35.0	41.0	32.5	41.0
28-29	36.62199294971646	37.0	35.0	41.0	33.0	41.0
30-31	36.4543612080051	36.0	35.0	40.0	32.5	41.0
32-33	36.05739904757039	36.0	35.0	40.0	31.0	41.0
34-35	35.78640010522162	36.0	35.0	40.0	30.5	41.0
36-37	35.52218023985748	36.0	35.0	40.0	30.0	41.0
38-39	35.50607959233143	36.0	35.0	40.0	30.0	41.0
40-41	35.36479673639049	36.0	35.0	40.0	29.5	41.0
42-43	35.28015854985472	35.5	35.0	40.0	29.5	41.0
44-45	35.113831751929155	35.0	34.5	40.0	29.0	41.0
46-47	34.99105290104059	35.0	34.0	40.0	28.5	41.0
48-49	34.94886968280246	35.5	34.5	40.0	28.0	41.0
50-51	34.75420370167061	35.0	34.0	40.0	28.0	41.0
52-53	34.56655974207658	35.0	34.0	40.0	27.5	41.0
54-55	34.383583361504755	35.0	34.0	39.0	27.0	41.0
56-57	34.05958337454876	35.0	34.0	39.0	26.0	41.0
58-59	33.707860533521455	35.0	34.0	38.5	25.0	40.0
60-61	33.36993661701612	35.0	33.0	37.5	25.0	40.0
62-63	32.84661122240786	35.0	32.0	37.0	23.0	40.0
64-65	32.55553864666297	35.0	32.0	36.5	22.0	40.0
66-67	32.32972032570875	35.0	32.0	36.0	21.0	39.0
68-69	32.04576271002203	35.0	32.0	35.5	19.5	39.0
70-71	31.76105452067094	35.0	32.0	35.0	19.0	38.5
72-73	31.518805104118318	35.0	32.0	35.0	18.0	37.5
74-75	31.25592822754828	35.0	31.5	35.0	17.5	37.0
76-77	29.165859387823204	32.5	28.0	34.5	11.5	35.0
78-79	30.101505169229632	34.0	29.5	35.0	14.0	36.0
80-81	30.616331852476677	34.0	31.0	35.0	15.0	36.0
82-83	30.514045673576	34.5	31.0	35.0	11.5	36.0
84-85	30.427422624605555	34.0	31.0	35.0	10.5	35.0
86	30.083426181270333	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	4.0
12	14.0
13	57.0
14	152.0
15	510.0
16	1215.0
17	2675.0
18	5139.0
19	7467.0
20	8550.0
21	8776.0
22	7968.0
23	7411.0
24	7991.0
25	9471.0
26	11189.0
27	13624.0
28	16761.0
29	20551.0
30	25160.0
31	30941.0
32	38085.0
33	51301.0
34	150280.0
35	128621.0
36	66340.0
37	109653.0
38	132751.0
39	57142.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.45477155059497	24.87523954767746	31.240386841644717	14.429602060082852
2	28.7238725905281	25.98224059011069	32.370758389196084	12.923128430165127
3	27.666221358902476	26.448454992211644	33.6105908607194	12.27473278816648
4	24.936002860984626	28.131457460789182	32.174228746156096	14.758310932070092
5	23.295393401691157	31.695187741246116	31.437459984803734	13.571958872258993
6	21.34085827364796	41.06480369319201	26.553567915231373	11.040770117928655
7	88.10973919603289	4.100925798102749	6.3072101812790295	1.4821248245853367
8	89.3577241693416	3.29448032149119	5.536744412547026	1.8110510966201903
9	86.0896579536351	5.00715789656758	6.823317894306618	2.07986625549071
10-11	50.87525259168032	23.26680529543036	16.120430930374376	9.737511182514949
12-13	32.83007033978541	32.76061102457382	21.536137866414194	12.87318076922659
14-15	12.398813865340237	36.66832253036264	38.35322724935677	12.579636354940362
16-17	14.00686766750402	21.97044881152829	38.59524785235928	25.42743566860841
18-19	23.421594129329115	26.29241610803913	31.763451356195848	18.522538406435913
20-21	26.880754986885343	27.827966994324772	27.74377882588756	17.547499192902325
22-23	19.242621714134156	27.639155052974957	27.56235848615651	25.55586474673438
24-25	15.720958342889876	26.539654312184297	38.52160358623119	19.217783758694644
26-27	14.573792641660589	39.99731510941201	28.3592383606732	17.0696538882542
28-29	12.388976513185856	29.66407344643208	40.778107380405515	17.168842659976544
30-31	23.092993957365675	31.96579645050942	28.983448247375165	15.957761344749736
32-33	28.63077102013885	30.42144086229555	25.649238067183138	15.298550050382461
34-35	18.00251749255133	31.1109251132926	28.924750234520303	21.961807159635768
36-37	23.778619357517638	29.55858007332904	31.12886061722048	15.53393995193285
38-39	17.098187644503092	33.32541634826618	28.369673562958507	21.206722444272216
40-41	19.225012310277695	26.910103993312774	28.503048492167622	25.361835204241913
42-43	21.51537616186738	25.160740160202096	27.771008181850792	25.552875496079736
44-45	17.342980098112644	37.16605993936712	25.603855807244425	19.887104155275807
46-47	21.803757325022854	32.621909794198245	26.264262801873556	19.310070078905348
48-49	21.462602300309904	24.88306594939144	32.9489338158165	20.705397934482146
50-51	17.765551440655766	35.33919298928326	26.982661259202818	19.912594310858154
52-53	24.020042110389223	25.712718881085433	29.69342245285952	20.573816555665825
54-55	22.896464314325684	28.59158466155704	30.323012990739844	18.18893803337743
56-57	24.410818695969294	22.533569284851673	29.79674182548646	23.258870193692573
58-59	25.359280753682484	27.29925007853577	26.99521611195233	20.34625305582942
60-61	25.90810717387547	30.43823501597347	25.768971143404677	17.88468666674638
62-63	24.691427807422688	26.072026809773874	31.525561354097935	17.7109840287055
64-65	16.875026495630802	33.78663054927209	31.38827322403184	17.95006973106527
66-67	15.67264118230842	30.892927215551058	23.302893703333723	30.131537898806798
68-69	15.968142197023141	36.2496643886765	22.003874068848422	25.77831934545194
70-71	18.013224444896153	31.699861842269744	28.322823852698427	21.96408986013568
72-73	23.91612488763135	31.985688554865792	24.920132657509054	19.178053899993806
74-75	23.12750621492386	35.54066848340639	20.91975438142621	20.41207092024353
76-77	31.727254248268682	28.647510823804872	22.699717271238082	16.925517656688367
78-79	17.430592317299716	31.7973657636231	30.92678727296641	19.845254646110767
80-81	14.076381332727511	29.574069826721296	32.912791057901245	23.436757782649956
82-83	17.594892403281438	25.907944123839766	34.74487560912776	21.75228786375104
84-85	13.551468917771691	26.23779434607696	31.65263168192634	28.55810505422501
86	17.19482196566601	25.059812188098867	35.18054530453942	22.5648205416957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2280.0
1	1884.0
2	2708.5
3	2421.0
4	1159.0
5	1855.5
6	3070.0
7	3834.0
8	3944.5
9	3889.0
10	3443.5
11	2970.0
12	2711.0
13	2604.0
14	2562.0
15	2601.5
16	2748.0
17	2893.5
18	3009.5
19	3115.0
20	3166.0
21	3189.0
22	3232.0
23	3382.0
24	3497.0
25	3832.5
26	4642.0
27	6316.0
28	7693.0
29	7878.0
30	8875.5
31	11911.0
32	12641.0
33	12906.0
34	16827.0
35	18419.5
36	17664.0
37	20212.0
38	23193.0
39	26961.0
40	35513.0
41	43129.5
42	51436.5
43	57344.0
44	58391.5
45	62815.5
46	67696.5
47	71013.0
48	67950.5
49	51152.5
50	39229.0
51	33240.0
52	24126.0
53	19057.0
54	17012.5
55	16954.0
56	17848.0
57	18700.0
58	16134.0
59	10953.0
60	6759.0
61	4446.5
62	3361.5
63	2276.5
64	1543.0
65	1222.0
66	679.5
67	322.5
68	215.5
69	154.5
70	50.5
71	36.0
72	54.0
73	47.0
74	18.0
75	9.5
76	5.5
77	9.0
78	7.0
79	2.0
80	2.0
81	2.5
82	2.5
83	2.0
84	2.0
85	3.0
86	4.0
87	2.5
88	1.5
89	1.0
90	0.5
91	1.5
92	3.5
93	5.0
94	3.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	919963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.261444350184167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52772766121477	13.727634453206608
2	9.047187893132131	3.123350606859204
3	2.83478367847979	1.467973821326678
4	1.3564526752792592	0.9365732947196547
5	0.8099695653890959	0.6990622289153368
6	0.5690697223111394	0.5893779205829094
7	0.44346589398699726	0.5358403295182858
8	0.31759820183709486	0.43857629493836575
9	0.26214759479480065	0.40725415071765725
>10	2.330832980483492	8.610082433335032
>50	0.8297817062616816	10.448950135547303
>100	1.6582946331559902	56.4855439275724
>500	0.010150234938980813	1.1508956814567401
>1k	0.0019031690510589027	0.7744181026890643
>5k	6.343896836863008E-4	0.6044666186147675
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5520	0.6000241314052848	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3057	0.3322959727728181	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2189	0.2379443521098131	No Hit
TAACAGGGGATGAGCACGGGATTTACAACTGTGACATCTCAAGATGGTCA	1826	0.1984862434684873	No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	975	0.10598252321017258	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC	969	0.10533032306734075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0870002380530522E-4	0.0	0.0	0.11630902547167658	0.0
2	1.0870002380530522E-4	0.0	0.0	0.3483835762960032	0.0
3	1.0870002380530522E-4	0.0	0.0	0.45393129941095456	0.0
4	1.0870002380530522E-4	0.0	0.0	0.6658963458312998	0.0
5	1.0870002380530522E-4	0.0	0.0	1.046781229245089	0.0
6	1.0870002380530522E-4	0.0	0.0	1.3841861031367566	0.0
7	1.0870002380530522E-4	0.0	0.0	1.6031079510806412	0.0
8	1.0870002380530522E-4	0.0	0.0	2.1021497603707977	0.0
9	1.0870002380530522E-4	0.0	0.0	2.284657100339905	0.0
10	1.0870002380530522E-4	0.0	0.0	2.640106178183253	0.0
11	1.0870002380530522E-4	0.0	0.0	3.2952412216578275	0.0
12	1.0870002380530522E-4	0.0	0.0	3.7542814221876313	0.0
13	1.0870002380530522E-4	0.0	0.0	3.9066788555626695	0.0
14	1.0870002380530522E-4	0.0	0.0	3.9637463680604546	0.0
15	1.0870002380530522E-4	0.0	0.0	4.0588588888900965	0.0
16	1.0870002380530522E-4	0.0	0.0	4.262345333453628	0.0
17	1.0870002380530522E-4	0.0	0.0	4.502572386063353	0.0
18	1.0870002380530522E-4	0.0	0.0	4.8059541525039595	0.0
19	1.0870002380530522E-4	0.0	0.0	4.975091389545014	0.0
20	1.0870002380530522E-4	0.0	0.0	5.136184824824476	0.0
21	1.0870002380530522E-4	0.0	0.0	5.332714467864468	0.0
22	1.0870002380530522E-4	0.0	0.0	5.542722913856318	0.0
23	1.0870002380530522E-4	0.0	0.0	5.752948759895778	0.0
24	1.0870002380530522E-4	0.0	0.0	5.902085192556657	0.0
25	1.0870002380530522E-4	0.0	0.0	6.028829420313643	0.0
26	1.0870002380530522E-4	0.0	0.0	6.154269247784965	0.0
27	1.0870002380530522E-4	0.0	0.0	6.277861174851597	0.0
28	1.0870002380530522E-4	0.0	0.0	6.411670904155928	0.0
29	1.0870002380530522E-4	0.0	0.0	6.550589534579108	0.0
30	1.0870002380530522E-4	0.0	0.0	6.7524454787855595	0.0
31	1.0870002380530522E-4	0.0	0.0	6.913865014136438	0.0
32	1.0870002380530522E-4	0.0	0.0	7.062566646702096	0.0
33	1.0870002380530522E-4	0.0	0.0	7.195724175863594	0.0
34	1.0870002380530522E-4	0.0	0.0	7.3523609101670395	0.0
35	1.0870002380530522E-4	0.0	0.0	7.563673756444553	0.0
36	1.0870002380530522E-4	0.0	0.0	7.720853990867024	0.0
37	1.0870002380530522E-4	0.0	0.0	7.898687229812503	0.0
38	1.0870002380530522E-4	0.0	0.0	8.048693262663825	0.0
39	1.0870002380530522E-4	0.0	0.0	8.226417801585498	0.0
40	1.0870002380530522E-4	0.0	0.0	8.44588314964841	0.0
41	1.0870002380530522E-4	0.0	0.0	8.624694688808137	0.0
42	1.0870002380530522E-4	0.0	0.0	8.817528531038748	0.0
43	1.0870002380530522E-4	0.0	0.0	8.988296268436883	0.0
44	1.0870002380530522E-4	0.0	0.0	9.175803809501033	0.0
45	1.0870002380530522E-4	0.0	0.0	9.370050752041115	0.0
46	1.0870002380530522E-4	0.0	0.0	9.564297694581196	0.0
47	1.0870002380530522E-4	0.0	0.0	9.764088338335347	0.0
48	1.0870002380530522E-4	0.0	0.0	9.960509281351532	0.0
49	1.0870002380530522E-4	0.0	0.0	10.18627923079515	0.0
50	1.0870002380530522E-4	0.0	0.0	10.403787978429568	0.0
51	1.0870002380530522E-4	0.0	0.0	10.5970566207554	0.0
52	1.0870002380530522E-4	0.0	0.0	10.82282657019902	0.0
53	1.0870002380530522E-4	0.0	0.0	11.041422318071488	0.0
54	1.0870002380530522E-4	0.0	0.0	11.268605367824575	0.0
55	1.0870002380530522E-4	0.0	0.0	11.524376523838459	0.0
56	1.0870002380530522E-4	0.0	0.0	11.765038376543405	0.0
57	1.0870002380530522E-4	0.0	0.0	11.991786626201272	0.0
58	1.0870002380530522E-4	0.0	0.0	12.187772769122237	0.0
59	1.0870002380530522E-4	0.0	0.0	12.407999017351784	0.0
60	1.0870002380530522E-4	0.0	0.0	12.654856771413632	0.0
61	1.0870002380530522E-4	0.0	0.0	12.910193127332295	0.0
62	1.0870002380530522E-4	0.0	0.0	13.180856186607505	0.0
63	1.0870002380530522E-4	0.0	0.0	13.51576095995165	0.0
64	1.0870002380530522E-4	0.0	0.0	13.774575716632082	0.0
65	1.0870002380530522E-4	0.0	0.0	14.040238574812248	0.0
66	1.0870002380530522E-4	0.0	0.0	14.26753032458914	0.0
67	1.0870002380530522E-4	0.0	0.0	14.529932182055148	0.0
68	1.0870002380530522E-4	0.0	0.0	14.802117041663632	0.0
69	1.0870002380530522E-4	0.0	0.0	15.074519301319727	0.0
70	1.0870002380530522E-4	0.0	0.0	15.306376452096442	0.0
71	1.0870002380530522E-4	0.0	0.0	15.558451807300946	0.0
72	1.0870002380530522E-4	0.0	0.0	15.820310164647926	0.0
73	1.0870002380530522E-4	0.0	0.0	16.123909331136144	0.0
74	2.1740004761061044E-4	0.0	0.0	16.430225998219495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	15	0.0013097073	80.00001	4
TAGCGCG	15	0.0013097073	80.00001	1
ATCGTAG	15	0.0013097073	80.00001	1
TCGTGAA	15	0.0013097073	80.00001	1
CGGGACG	30	4.0592568E-8	80.00001	6
GCGGACC	15	0.0013097073	80.00001	5
CCGAACG	30	4.0592568E-8	80.00001	1
CACGTTG	30	4.0592568E-8	80.00001	1
TATCGTG	15	0.0013097073	80.00001	1
CGATATC	30	4.0592568E-8	80.00001	9
ATACCGG	15	0.0013097073	80.00001	2
CTAGACG	20	4.0904655E-5	80.0	1
ACCGGGT	50	0.0	80.0	4
TTGTGCG	40	4.1836756E-11	80.0	1
ATTACCG	55	0.0	80.0	1
GCACGAG	20	4.0904655E-5	80.0	1
ACGGGTA	40	4.1836756E-11	80.0	5
GCCGCAC	35	1.2896635E-9	80.0	8
TAGGCGA	40	4.1836756E-11	80.0	1
TGGGCGA	165	0.0	77.57575	6
>>END_MODULE