FastQCFastQC Report
Thu 26 May 2016
SRR1547779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547779_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5014673
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309710.6176075688285159No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAA234870.4683655345024491No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCT78460.15646084998962045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150900.067.8064961
TAGGGAC60400.066.887425
TAATGCG5350.065.7943951
AGGGATT115750.065.6933066
TACGGGA27550.064.319424
GGGCGAT29250.064.136767
CGAACGG2200.063.6363642
CTTGACC54600.063.076927
ACGGGAT30050.062.961735
TTAGGGA87200.062.889914
GCGGGAT46200.062.59745
AAGGGAT100750.062.570725
CGAGGGA38300.062.4543084
TAAGGGA75550.062.26344
TAGGGAT96000.062.2083325
CGAAACG5200.061.53846480
CTCAACT57000.061.4736822
ACTTGAC56350.061.2599836
ATAGGGA77000.061.2467544
TAGGGAG73250.061.1604085
Produced by FastQC (version 0.11.5)