FastQCFastQC Report
Thu 26 May 2016
SRR1547778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547778_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3799186
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187100.49247391414897823No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA60120.15824442393712757No Hit
GTCTAAGGGTCATTTGCTCTGTGGCATTTCTTTGTTTGTTTGTTTTTTTC58130.1530064598048108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC1400.074.2857069
CGTTTTT111100.069.01891
ATACGCG1400.068.571431
AATCGCG1000.068.01
TACGCGG2900.067.5862052
ACGGGTA6100.066.229515
ATTAGCG4050.066.1728441
CGAGGGT7700.065.4545444
TACGGGA13650.065.3479844
CCGATCC23250.064.6881780
CGTAAGG9400.064.680852
TAATGCG2000.064.01
ACGAGGG22400.063.9285663
CGAGGGA21900.063.1963464
CGCGGGT6250.061.444
GTACGGG16950.060.6489643
TATTGCG3100.060.6451571
GCGAGGG32100.060.560753
AATGCGG9250.060.540542
CGGGATC11300.060.530976
Produced by FastQC (version 0.11.5)