FastQCFastQC Report
Thu 26 May 2016
SRR1547777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547777_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4003824
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273180.6822977233764521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149800.071.535381
TACGGGA16400.066.0975654
TAGCGGG25250.065.584163
TACGCGG2050.064.390242
CGTTAGG5700.063.859652
CCGATCC36900.063.3062380
CGATAGG11050.062.624432
TCGATAG3950.061.7721561
ATAGCGG7450.061.744972
TTAGGGA58300.061.475134
GGGCGAT24600.061.4634137
GTACGGG12200.061.3114783
ACGGGAT21900.061.1872145
TAACGGG13500.061.0370333
TAGGGAC42800.060.65425
ATAGGGA63650.060.644154
AGGGTAA34200.060.5847936
CGAGGGA29600.059.729734
GCGAGGG37900.058.997363
TAAGGGA54950.058.9626924
Produced by FastQC (version 0.11.5)