##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547777_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4003824
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80783221240494	31.0	31.0	33.0	30.0	34.0
2	32.25725581344235	33.0	31.0	34.0	31.0	34.0
3	32.411397703795174	34.0	31.0	34.0	31.0	34.0
4	36.01042803080255	37.0	35.0	37.0	35.0	37.0
5	35.978162127006584	37.0	35.0	37.0	35.0	37.0
6	36.03611647265215	37.0	35.0	37.0	35.0	37.0
7	36.262868697525164	37.0	35.0	37.0	35.0	37.0
8	36.21442975515407	37.0	36.0	37.0	35.0	37.0
9	38.095598607731006	39.0	39.0	39.0	37.0	39.0
10-11	37.58157239179344	39.0	37.5	39.0	35.0	39.0
12-13	37.10744178565292	39.0	37.0	39.0	33.5	39.0
14-15	38.27105824331939	40.0	37.0	41.0	33.0	41.0
16-17	38.35667888998118	40.0	37.0	41.0	34.0	41.0
18-19	38.21415851446018	40.0	37.0	41.0	34.0	41.0
20-21	38.05457182933116	40.0	35.5	41.0	34.0	41.0
22-23	37.87569958120037	40.0	35.0	41.0	33.0	41.0
24-25	37.79122196679974	40.0	35.0	41.0	33.0	41.0
26-27	37.77248225696235	40.0	35.0	41.0	33.0	41.0
28-29	37.73996272063907	40.0	35.0	41.0	33.0	41.0
30-31	37.555295887131905	40.0	35.0	41.0	33.0	41.0
32-33	37.256302100192215	40.0	35.0	41.0	32.5	41.0
34-35	36.950813397392096	40.0	35.0	41.0	31.0	41.0
36-37	36.76457144469887	40.0	35.0	41.0	31.0	41.0
38-39	36.64952542869017	39.5	35.0	41.0	31.0	41.0
40-41	36.49680293139758	39.0	35.0	41.0	30.0	41.0
42-43	36.45270334060638	39.0	35.0	41.0	30.0	41.0
44-45	36.35061843377731	39.0	35.0	41.0	30.0	41.0
46-47	36.27231441741695	39.0	35.0	41.0	30.0	41.0
48-49	36.150124106354326	39.0	35.0	41.0	30.0	41.0
50-51	35.97998138779327	38.5	35.0	40.5	29.5	41.0
52-53	35.789626616954195	38.0	35.0	40.0	29.0	41.0
54-55	35.59627770851066	38.0	35.0	40.0	28.5	41.0
56-57	35.31222638657444	37.0	34.5	40.0	28.0	41.0
58-59	35.03674724463413	37.0	34.0	40.0	27.0	41.0
60-61	34.745440109255554	36.5	34.0	40.0	26.5	41.0
62-63	34.42232950299514	36.0	34.0	39.0	26.0	41.0
64-65	34.13152226471493	35.0	34.0	39.0	26.0	40.5
66-67	33.840720895823594	35.0	34.0	38.5	26.0	40.0
68-69	33.39699709577644	35.0	33.0	37.5	24.5	40.0
70-71	33.15017118634585	35.0	33.0	37.0	25.0	39.5
72-73	32.897770356539155	35.0	33.0	37.0	25.0	39.0
74-75	32.59320489611931	35.0	33.0	36.0	24.0	39.0
76-77	30.546431361618293	33.0	30.0	34.5	20.0	36.0
78-79	31.727290834961778	34.5	31.5	35.0	22.5	37.0
80-81	31.87361582327295	35.0	32.0	35.0	23.0	37.0
82-83	31.713611287609044	35.0	32.0	35.0	22.5	36.0
84-85	31.497761514991666	35.0	32.0	35.0	20.0	36.0
86	31.079664590651337	34.0	31.0	35.0	20.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	28.0
10	31.0
11	51.0
12	92.0
13	207.0
14	612.0
15	1700.0
16	4157.0
17	9454.0
18	20014.0
19	30321.0
20	34796.0
21	33461.0
22	29397.0
23	26212.0
24	26023.0
25	28327.0
26	33524.0
27	40596.0
28	50003.0
29	62235.0
30	78239.0
31	97578.0
32	124639.0
33	166391.0
34	352006.0
35	350149.0
36	373482.0
37	646387.0
38	916726.0
39	465327.0
40	1644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.14816035869709	23.792229628475177	29.381311466238273	13.67829854658946
2	31.38047526564604	24.990234335974808	30.860497364519517	12.76879303385963
3	29.296667385979003	24.65772721278458	33.12228509544875	12.923320305787666
4	26.028266976770208	27.310441218195404	31.9762307234284	14.685061081605985
5	23.78423726917067	31.868558657923025	29.89479557542989	14.452408497476412
6	22.113859150651976	40.43264639005111	27.054810601065384	10.39868385823153
7	88.15609777053137	3.440735656712183	6.8111135754218965	1.5920529973345483
8	88.76214339091828	2.9327462945424174	6.601164286941684	1.7039460275976168
9	84.12974696190442	5.571523623416015	7.974776114034983	2.323953300644584
10-11	33.97811692022427	30.032126786791828	23.134957980170956	12.854798312812951
12-13	27.499435539624116	24.919339611331566	29.55392145109276	18.02730339795156
14-15	18.773427603211328	29.85649469107533	33.47368665555729	17.89639105015605
16-17	23.689015800894346	27.020418479933184	31.307844700466354	17.982721018706116
18-19	23.988304680725225	28.573845903316432	30.03775890248922	17.400090513469124
20-21	25.769988890620567	27.679088791115692	30.01376933651429	16.537152981749447
22-23	22.501501064981877	23.89118502711408	33.30734567753228	20.299968230371764
24-25	20.89022894113228	27.840896602847675	32.741161949176586	18.527712506843457
26-27	19.69710706564524	31.10492619056182	30.052270029851464	19.145696713941472
28-29	18.119128113523473	29.196925239471067	33.66605275356759	19.017893893437872
30-31	22.481732463764644	27.46533064390443	31.467529541758076	18.58540735057285
32-33	23.67892544727241	28.04879784925611	29.802608706076988	18.469667997394488
34-35	20.496817542429437	28.66331037528123	30.912834829902614	19.927037252386718
36-37	22.396513932680357	29.36626335223526	29.495402395310084	18.7418203197743
38-39	21.05222407378546	30.265428750114893	28.123076838542353	20.559270337557294
40-41	20.88853056478007	28.00080622924484	29.457051558709875	21.653611647265215
42-43	21.171809749879117	27.655548795351642	29.216981066100807	21.955660388668434
44-45	20.651194957620515	29.771613337649207	28.83279834478239	20.744393359947892
46-47	21.599738150328285	29.402041648184333	28.821246887975093	20.176973313512285
48-49	21.272013954659347	28.78689223102714	30.00031969437218	19.940774119941338
50-51	20.29382410415643	29.740155910949134	29.648793253649515	20.31722673124493
52-53	21.56327301100148	27.677003284859676	29.60477283716767	21.154950866971177
54-55	21.1442610864014	27.520989933623458	30.808871718636983	20.52587726133816
56-57	21.315784609912924	27.861127262337206	29.704365126938647	21.118723000811222
58-59	21.774246320517584	28.704346145085296	29.180116308808778	20.341291225588336
60-61	22.575417900487135	29.538723480352786	28.186079108372397	19.69977951078769
62-63	22.531647245233557	27.387505045176813	29.662954715292177	20.41789299429745
64-65	21.527794428526327	29.293632787055575	29.381111657255666	19.797461127162432
66-67	20.33012689868486	29.75351813666135	27.725369546713342	22.19098541794045
68-69	20.15190977425581	31.055873085330425	27.26921812747014	21.522999012943625
70-71	20.210353901670004	29.583805382054756	29.40130485256095	20.80453586371429
72-73	21.739329700806028	29.976342616458666	28.237005422815788	20.047322259919518
74-75	20.488313172606986	31.376166884458456	27.01649722864941	21.119022714285144
76-77	22.36002381723073	29.153104631971832	27.950092211845472	20.536779338951963
78-79	20.009908027925302	29.275824811480224	28.899821770387508	21.814445390206963
80-81	19.118560156490393	29.223399929667238	29.15446583066588	22.503574083176485
82-83	20.185864813238545	29.23289085634134	29.689641702532377	20.89160262788774
84-85	18.849842050999243	28.27240907692246	29.308630948812937	23.56911792326536
86	19.40130235494867	28.617691486938483	29.158998996958907	22.822007161153937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1734.0
1	2253.0
2	9224.0
3	10329.5
4	5381.0
5	6652.0
6	8072.0
7	8619.0
8	8959.0
9	9413.0
10	9458.0
11	9620.5
12	9792.0
13	9864.0
14	9996.0
15	10152.5
16	10841.5
17	10993.0
18	11030.0
19	11797.5
20	12440.0
21	12733.0
22	13814.0
23	16219.0
24	20442.5
25	25806.0
26	32196.0
27	42219.0
28	48975.5
29	49635.0
30	59217.0
31	73182.0
32	77635.5
33	86284.5
34	98367.0
35	114934.5
36	127998.0
37	122767.5
38	123295.5
39	135981.0
40	151339.0
41	164958.0
42	178665.5
43	187185.0
44	185839.5
45	187527.0
46	194383.0
47	200568.0
48	204302.5
49	194351.0
50	183027.0
51	164859.5
52	140938.5
53	128224.5
54	118091.5
55	110089.0
56	95743.5
57	86228.0
58	79139.5
59	61811.0
60	47268.5
61	38483.0
62	29729.0
63	21410.5
64	17363.0
65	14756.0
66	10078.0
67	7091.5
68	5446.5
69	3889.0
70	2444.5
71	1828.0
72	1510.5
73	935.0
74	453.5
75	214.5
76	201.0
77	214.0
78	153.5
79	82.0
80	61.5
81	41.0
82	40.5
83	27.0
84	15.5
85	10.5
86	4.0
87	8.5
88	7.0
89	7.0
90	10.0
91	4.5
92	1.5
93	1.0
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	4003824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.172672031772933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30058251916002	12.183744025235335
2	7.585243587718141	2.3017682647511197
3	2.200656102224148	1.001694998813003
4	1.0944122545445762	0.664206328230322
5	0.7460638955574351	0.5659891401019929
6	0.534772280580534	0.4868354654959018
7	0.3955390724700477	0.42009692356377903
8	0.3256648407288902	0.39529646565272153
9	0.2750126980711988	0.37554097251665625
>10	3.1371637281655698	11.463146721435804
>50	1.4311081436396427	15.837996133922728
>100	1.961760971180771	51.89036874202168
>500	0.008847986331101175	0.9509795654804735
>1k	0.003004976489807946	0.7703785559483037
>5k	0.0	0.0
>10k+	1.6694313832266364E-4	0.6919576968302676
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27318	0.6822977233764521	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.492836847973338E-5	0.0	0.0	0.021929035841735302	0.0
2	7.492836847973338E-5	0.0	0.0	0.0726555413025148	0.0
3	7.492836847973338E-5	0.0	0.0	0.10949532247171703	0.0
4	7.492836847973338E-5	0.0	0.0	0.15365310762910658	0.0
5	7.492836847973338E-5	0.0	0.0	0.25772861144745624	0.0
6	7.492836847973338E-5	0.0	0.0	0.35086457346776484	0.0
7	7.492836847973338E-5	0.0	0.0	0.4157275644483873	0.0
8	7.492836847973338E-5	0.0	0.0	0.5527465742749931	0.0
9	7.492836847973338E-5	0.0	0.0	0.6071195936684529	0.0
10	7.492836847973338E-5	0.0	0.0	0.7093468643976358	0.0
11	7.492836847973338E-5	0.0	0.0	0.8907484444870704	0.0
12	7.492836847973338E-5	0.0	0.0	1.0224725162744417	0.0
13	7.492836847973338E-5	0.0	0.0	1.0728243798928225	0.0
14	7.492836847973338E-5	0.0	0.0	1.0987995476324635	0.0
15	7.492836847973338E-5	0.0	0.0	1.1360389467668908	0.0
16	7.492836847973338E-5	0.0	0.0	1.2107924823868381	0.0
17	7.492836847973338E-5	0.0	0.0	1.298958195964658	0.0
18	7.492836847973338E-5	0.0	0.0	1.397139334795935	0.0
19	7.492836847973338E-5	0.0	0.0	1.4673971683070983	0.0
20	7.492836847973338E-5	0.0	0.0	1.5440488892618656	0.0
21	7.492836847973338E-5	0.0	0.0	1.6365104959658567	0.0
22	7.492836847973338E-5	0.0	0.0	1.7375139366765373	0.0
23	7.492836847973338E-5	0.0	0.0	1.8389419714752697	0.0
24	7.492836847973338E-5	0.0	0.0	1.9217877708910283	0.0
25	7.492836847973338E-5	0.0	0.0	1.996816043862068	0.0
26	7.492836847973338E-5	0.0	0.0	2.068847182093918	0.0
27	9.990449130631117E-5	0.0	0.0	2.1584615107956795	0.0
28	9.990449130631117E-5	0.0	0.0	2.240283289175548	0.0
29	9.990449130631117E-5	0.0	0.0	2.341736300097107	0.0
30	9.990449130631117E-5	0.0	0.0	2.445087496353486	0.0
31	9.990449130631117E-5	0.0	0.0	2.5396970496205626	0.0
32	9.990449130631117E-5	0.0	0.0	2.6257397927581234	0.0
33	9.990449130631117E-5	0.0	0.0	2.723171647904603	0.0
34	9.990449130631117E-5	0.0	0.0	2.826822557634901	0.0
35	9.990449130631117E-5	0.0	0.0	2.944235311042643	0.0
36	9.990449130631117E-5	0.0	0.0	3.0487603850718714	0.0
37	9.990449130631117E-5	0.0	0.0	3.1676966819720347	0.0
38	9.990449130631117E-5	0.0	0.0	3.2936512693864666	0.0
39	9.990449130631117E-5	0.0	0.0	3.4830701849032324	0.0
40	9.990449130631117E-5	0.0	0.0	3.6243101594875298	0.0
41	9.990449130631117E-5	0.0	0.0	3.766474250616411	0.0
42	9.990449130631117E-5	0.0	0.0	3.9136835185562604	0.0
43	9.990449130631117E-5	0.0	0.0	4.041860980902258	0.0
44	9.990449130631117E-5	0.0	0.0	4.1683400668960475	0.0
45	9.990449130631117E-5	0.0	0.0	4.2995646162268875	0.0
46	1.2488061413288895E-4	0.0	0.0	4.437932336686128	0.0
47	1.2488061413288895E-4	0.0	0.0	4.58716467057493	0.0
48	1.2488061413288895E-4	0.0	0.0	4.73247575317996	0.0
49	1.2488061413288895E-4	0.0	0.0	4.884055842614461	0.0
50	1.2488061413288895E-4	0.0	0.0	5.043653267476293	0.0
51	1.2488061413288895E-4	0.0	0.0	5.212192144310039	0.0
52	1.4985673695946676E-4	0.0	0.0	5.398938614684361	0.0
53	1.4985673695946676E-4	0.0	0.0	5.567402563149629	0.0
54	1.4985673695946676E-4	0.0	0.0	5.734367944245301	0.0
55	1.4985673695946676E-4	0.0	0.0	5.90048413716487	0.0
56	1.4985673695946676E-4	0.0	0.0	6.076590779215071	0.0
57	1.4985673695946676E-4	0.0	0.0	6.258342025024077	0.0
58	1.4985673695946676E-4	0.0	0.0	6.434049049109052	0.0
59	1.7483285978604453E-4	0.0	0.0	6.621494850922518	0.0
60	1.7483285978604453E-4	0.0	0.0	6.815958943250253	0.0
61	1.7483285978604453E-4	0.0	0.0	7.026957228889182	0.0
62	1.7483285978604453E-4	0.0	0.0	7.284835697073597	0.0
63	1.7483285978604453E-4	0.0	0.0	7.508097259020377	0.0
64	1.7483285978604453E-4	0.0	0.0	7.728136901122527	0.0
65	1.7483285978604453E-4	0.0	0.0	7.937661595514688	0.0
66	1.7483285978604453E-4	0.0	0.0	8.144189155167659	0.0
67	1.7483285978604453E-4	0.0	0.0	8.36145644763606	0.0
68	1.7483285978604453E-4	0.0	0.0	8.605648000511511	0.0
69	1.9980898261262234E-4	0.0	0.0	8.936631580209319	0.0
70	2.2478510543920012E-4	0.0	0.0	9.174753935237913	0.0
71	2.2478510543920012E-4	0.0	0.0	9.406357522208769	0.0
72	2.2478510543920012E-4	0.0	0.0	9.638160918162237	0.0
73	2.497612282657779E-4	0.0	0.0	9.872811592117936	0.0
74	2.497612282657779E-4	0.0	0.0	10.13413676525242	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14980	0.0	71.53538	1
TACGGGA	1640	0.0	66.097565	4
TAGCGGG	2525	0.0	65.58416	3
TACGCGG	205	0.0	64.39024	2
CGTTAGG	570	0.0	63.85965	2
CCGATCC	3690	0.0	63.30623	80
CGATAGG	1105	0.0	62.62443	2
TCGATAG	395	0.0	61.772156	1
ATAGCGG	745	0.0	61.74497	2
TTAGGGA	5830	0.0	61.47513	4
GGGCGAT	2460	0.0	61.463413	7
GTACGGG	1220	0.0	61.311478	3
ACGGGAT	2190	0.0	61.187214	5
TAACGGG	1350	0.0	61.037033	3
TAGGGAC	4280	0.0	60.6542	5
ATAGGGA	6365	0.0	60.64415	4
AGGGTAA	3420	0.0	60.584793	6
CGAGGGA	2960	0.0	59.72973	4
GCGAGGG	3790	0.0	58.99736	3
TAAGGGA	5495	0.0	58.962692	4
>>END_MODULE