FastQCFastQC Report
Thu 26 May 2016
SRR1547776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547776_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2107479
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127570.6053203851616078No Hit
AAAATAGGGGAAGCAAGTTTCATGGAATTTTGAAAGTTTTTAAAATTATA21910.10396307626315611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG800.080.01
CTACGCG850.075.294121
CGTTAGG4550.073.846162
TTAGGCG650.073.846151
TCGTAAG950.071.578951
CGTTTTT68600.071.1953351
GTTAGCG900.066.6666641
ACGGGAT12950.065.7915045
TACGGGA9400.065.531914
AGGGATT45650.065.4545446
TACGCGG1900.065.263162
CGTAAGG3650.064.657542
GGGCGAT14800.064.3243267
CGAGGGA13950.064.229394
GCTAACG503.0013325E-1064.01
TGTAGGG30700.063.0618863
GCGAGGG17550.062.9059873
CTCGCGG700.062.8571431
GCGTAAG1400.062.8571431
ATTACCG459.427822E-962.222221
Produced by FastQC (version 0.11.5)