##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547776_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2107479
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.825747255370043	31.0	31.0	33.0	30.0	34.0
2	32.2751533941738	33.0	31.0	34.0	31.0	34.0
3	32.4162148234929	34.0	31.0	34.0	31.0	34.0
4	36.00156347939885	37.0	35.0	37.0	35.0	37.0
5	35.95924467100265	37.0	35.0	37.0	35.0	37.0
6	36.00238578889754	37.0	35.0	37.0	35.0	37.0
7	36.268089504094704	37.0	35.0	37.0	35.0	37.0
8	36.23317812419483	37.0	36.0	37.0	35.0	37.0
9	38.10891164277319	39.0	39.0	39.0	37.0	39.0
10-11	37.58369668214962	39.0	37.5	39.0	35.0	39.0
12-13	36.807456681656134	39.0	35.0	39.0	33.0	39.0
14-15	37.8637115719777	40.0	35.0	41.0	33.0	41.0
16-17	38.01996413724645	40.0	35.0	41.0	34.0	41.0
18-19	37.89289430642013	40.0	36.0	41.0	34.0	41.0
20-21	37.711404953501315	40.0	35.0	41.0	33.5	41.0
22-23	37.53232464000827	39.0	35.0	41.0	33.0	41.0
24-25	37.464079357374374	39.0	35.0	41.0	33.0	41.0
26-27	37.44333893718514	39.0	35.0	41.0	33.0	41.0
28-29	37.43177156213656	39.0	35.0	41.0	33.0	41.0
30-31	37.27481246550974	39.0	35.0	41.0	33.0	41.0
32-33	36.96361624481193	39.0	35.0	41.0	32.0	41.0
34-35	36.68398095544487	39.0	35.0	41.0	31.0	41.0
36-37	36.5063296004373	39.0	35.0	41.0	31.0	41.0
38-39	36.43118413042313	39.0	35.0	41.0	31.0	41.0
40-41	36.276613195196724	39.0	35.0	41.0	30.0	41.0
42-43	36.230557457512035	38.5	35.0	41.0	30.0	41.0
44-45	36.1230211546592	38.0	35.0	41.0	30.0	41.0
46-47	36.049278071098215	38.0	35.0	41.0	30.0	41.0
48-49	35.96050114852865	38.0	35.0	40.5	30.0	41.0
50-51	35.76682353655718	38.0	35.0	40.0	29.5	41.0
52-53	35.59425099846784	37.5	35.0	40.0	29.0	41.0
54-55	35.40986553128169	37.0	35.0	40.0	29.0	41.0
56-57	35.12216966337506	36.5	34.5	40.0	28.0	41.0
58-59	34.81999298688148	36.0	34.0	40.0	27.0	41.0
60-61	34.52117435096625	36.0	34.0	39.0	26.5	41.0
62-63	34.1522363923911	35.0	33.5	39.0	26.0	41.0
64-65	33.885568492022934	35.0	33.5	39.0	26.0	40.0
66-67	33.620507487856344	35.0	33.0	38.0	25.5	40.0
68-69	33.21467924472795	35.0	33.0	37.0	24.5	40.0
70-71	32.988826460429735	35.0	33.0	37.0	24.5	39.0
72-73	32.72891663451925	35.0	33.0	36.0	24.0	39.0
74-75	32.43884850098151	35.0	33.0	36.0	23.5	38.5
76-77	30.377043614669468	33.0	29.5	34.5	19.5	36.0
78-79	31.4804351549885	34.0	31.5	35.0	20.0	37.0
80-81	31.70812022326201	35.0	32.0	35.0	20.0	37.0
82-83	31.577472658090542	35.0	32.0	35.0	20.0	36.0
84-85	31.379390731770044	35.0	32.0	35.0	20.0	36.0
86	30.95326738724324	34.0	31.0	35.0	19.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	12.0
10	10.0
11	23.0
12	69.0
13	109.0
14	296.0
15	818.0
16	2030.0
17	4538.0
18	8904.0
19	13750.0
20	16569.0
21	16939.0
22	15498.0
23	14426.0
24	14868.0
25	16872.0
26	19665.0
27	23941.0
28	29346.0
29	36453.0
30	45846.0
31	56421.0
32	70939.0
33	95919.0
34	234221.0
35	221771.0
36	176901.0
37	307502.0
38	435412.0
39	226554.0
40	851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.447051192443677	23.88090225335579	30.604385619026335	14.067660935174207
2	29.44399445973127	25.544453823739172	31.602450131175686	13.409101585353875
3	28.0753449975065	25.346207482968985	33.83995759862851	12.73848992089601
4	25.891456095173425	27.269927719327214	32.787752570725495	14.05086361477386
5	24.57604559760738	31.334689455980346	30.32257972677308	13.7666852196392
6	23.0570743528168	39.51446254031475	27.195098978447707	10.233364128420734
7	88.57023011854449	3.37403124776095	6.578428539501462	1.4773100941931094
8	89.47050006192232	2.8640854784318135	6.101365660108595	1.5640487995372672
9	84.70670407629211	5.800342494515959	7.305505772536761	2.187447656655179
10-11	39.3606769035421	28.72645468827922	20.233890824060406	11.678977584118275
12-13	29.67351038847837	25.739734535907594	27.785591220600537	16.801163855013503
14-15	15.97102509680998	31.383159689847446	35.517127335551145	17.128687877791428
16-17	21.617226079121075	26.255469212267357	32.93178722065558	19.19551748795599
18-19	23.897343698323922	27.766468847376412	30.823509985152874	17.512677469146787
20-21	25.677029284752066	28.06846473915043	30.247110410115592	16.007395565981916
22-23	21.43141165344945	25.00478059330603	32.28046400462353	21.283343748620982
24-25	19.360786038674643	27.336334074977735	35.26089702435944	18.04198286198819
26-27	17.585821732980495	34.470023188843165	30.238260974367954	17.70589410380839
28-29	16.430436554765198	29.849621277365042	35.58151706375247	18.13842510411729
30-31	21.50754527091373	30.014462777565043	31.06448510281716	17.41350684870407
32-33	25.520396644521725	28.192902515280103	29.24783592149673	17.038864918701442
34-35	19.412459151431637	29.252462302115468	30.82206275839522	20.513015788057675
36-37	22.467270136499582	30.097049602866743	29.96010873655206	17.475571524081616
38-39	19.743114878013017	31.40671864345979	28.46621484721793	20.383951631309255
40-41	20.337640375064236	27.72347909516536	29.44743458890931	22.491445940861094
42-43	20.508745282871146	27.402835330743507	29.245226168327182	22.843193218058165
44-45	19.233667334288977	31.1738337606211	29.022614222964975	20.569884682124947
46-47	20.32777076307759	31.15354886098509	28.39959496630809	20.119085409629232
48-49	21.016176199145995	27.42217122922696	31.661833878297248	19.899818693329806
50-51	18.821041633155065	31.59205856855513	29.45697205049256	20.12992774779725
52-53	21.68579141239367	27.673917509972817	30.487041626512056	20.153249451121457
54-55	21.000541405157534	28.311266684033388	31.294428082082902	19.393763828726172
56-57	21.845840456773235	26.35886288783898	29.78096104397719	22.014335611410598
58-59	23.261062150559983	28.718293278367184	28.553689028455327	19.466955542617505
60-61	23.3274685062105	29.849858527653183	27.517806820376382	19.304866145759934
62-63	22.207552246072204	26.82814395778084	31.377489407960883	19.586814388186074
64-65	19.636803023897272	30.638193785086354	30.446210851923077	19.278792339093297
66-67	19.41082212444347	29.29697045617062	27.210757497464982	24.08144992192093
68-69	18.152517771232834	31.883496822506892	26.956804789039417	23.00718061722086
70-71	19.099692096576053	30.27126248944829	30.004403365347887	20.624642048627766
72-73	22.05561716154704	30.7526195990565	27.700584442359805	19.49117879703665
74-75	20.95565365064136	32.39645567049541	25.85214372242855	20.79574695643468
76-77	23.98291987725619	29.128214326216302	27.046343047783633	19.84252274874388
78-79	19.456160654507116	29.65528956634918	29.961769488569047	20.926780290574662
80-81	17.63573919360525	28.980312496589526	30.94657170961134	22.437376600193883
82-83	18.763603338396255	28.262535474849333	31.25891171394828	21.714949472806136
84-85	17.59493214404509	27.893587551762085	29.921887715132627	24.5895925890602
86	18.395248541029353	28.58922912161877	30.66602324388523	22.349499093466648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1525.0
1	1980.5
2	5296.0
3	5366.0
4	2859.0
5	3668.0
6	4642.0
7	5090.0
8	5296.0
9	5539.0
10	5306.5
11	5252.0
12	5287.5
13	5154.5
14	5201.0
15	5264.5
16	5255.0
17	5157.5
18	5527.5
19	6144.5
20	7046.5
21	7726.0
22	7240.0
23	8513.5
24	11228.0
25	14436.0
26	17975.5
27	22545.0
28	27253.5
29	28679.0
30	32471.5
31	39300.0
32	46637.5
33	50747.0
34	52891.5
35	57702.0
36	60176.0
37	62821.0
38	66913.5
39	71866.0
40	84780.5
41	96019.0
42	102577.5
43	107307.0
44	107525.5
45	107946.0
46	111199.5
47	114775.5
48	110509.0
49	98144.5
50	90571.0
51	77537.5
52	62492.0
53	56957.0
54	52399.5
55	50848.5
56	47104.0
57	43876.0
58	39722.5
59	30249.5
60	21070.0
61	15352.0
62	12360.0
63	9523.5
64	7821.0
65	5855.0
66	3440.5
67	2518.5
68	1798.0
69	1276.0
70	834.0
71	667.0
72	529.5
73	282.5
74	173.5
75	116.0
76	49.5
77	43.5
78	33.5
79	21.0
80	20.0
81	23.0
82	18.0
83	9.5
84	7.5
85	4.5
86	4.0
87	3.5
88	2.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2107479.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.97941667052392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06593521247977	12.794069398753875
2	7.8987136133055955	2.524336719762993
3	2.3489020369169706	1.1260225309841592
4	1.168714543663805	0.7470150664842065
5	0.7295334044914835	0.582875912271764
6	0.52818497345364	0.5064052661955198
7	0.40249860565075996	0.4502185050298871
8	0.30365662537780674	0.3881804593341729
9	0.2620753811611149	0.37690305431938464
>10	2.936841288356554	11.364857031946636
>50	1.4321458703844787	16.82888127979529
>100	1.9141169633790023	50.48370477825297
>500	0.00598722853730266	0.6285677961098141
>1k	0.0023948914149210638	0.587715672983651
>5k	0.0	0.0
>10k+	2.9936142686513297E-4	0.6102465277757152
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12757	0.6053203851616078	No Hit
AAAATAGGGGAAGCAAGTTTCATGGAATTTTGAAAGTTTTTAAAATTATA	2191	0.10396307626315611	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038766697082153606	0.0
2	0.0	0.0	0.0	0.1467630282436978	0.0
3	0.0	0.0	0.0	0.21262370823149365	0.0
4	0.0	0.0	0.0	0.3036803688198079	0.0
5	0.0	0.0	0.0	0.5038247118951126	0.0
6	0.0	0.0	0.0	0.6613589032203879	0.0
7	0.0	0.0	0.0	0.7560692182460655	0.0
8	0.0	0.0	0.0	0.9477200010059412	0.0
9	0.0	0.0	0.0	1.0198915386582736	0.0
10	0.0	0.0	0.0	1.1621468114273026	0.0
11	0.0	0.0	0.0	1.4344152420973115	0.0
12	0.0	0.0	0.0	1.6263981752605838	0.0
13	0.0	0.0	0.0	1.7031249184452135	0.0
14	0.0	0.0	0.0	1.7380481608594913	0.0
15	0.0	0.0	0.0	1.789341673155462	0.0
16	0.0	0.0	0.0	1.8959619526457916	0.0
17	0.0	0.0	0.0	2.033519669709639	4.745005762809499E-5
18	0.0	0.0	0.0	2.177815294956676	4.745005762809499E-5
19	0.0	0.0	0.0	2.2869978775589224	4.745005762809499E-5
20	0.0	0.0	0.0	2.403677569266408	4.745005762809499E-5
21	0.0	0.0	0.0	2.536727530855586	4.745005762809499E-5
22	0.0	0.0	0.0	2.679884354719549	4.745005762809499E-5
23	0.0	0.0	0.0	2.8275014840005523	4.745005762809499E-5
24	0.0	0.0	0.0	2.9460791780131617	4.745005762809499E-5
25	0.0	0.0	0.0	3.046720750242351	4.745005762809499E-5
26	0.0	0.0	0.0	3.1482164235088463	4.745005762809499E-5
27	0.0	0.0	0.0	3.2645165147553072	4.745005762809499E-5
28	0.0	0.0	0.0	3.3742684980490907	4.745005762809499E-5
29	4.745005762809499E-5	0.0	0.0	3.5118262151129382	4.745005762809499E-5
30	9.490011525618998E-5	0.0	0.0	3.655220289265041	4.745005762809499E-5
31	9.490011525618998E-5	0.0	0.0	3.786419698606724	4.745005762809499E-5
32	9.490011525618998E-5	0.0	0.0	3.9030519402565815	4.745005762809499E-5
33	9.490011525618998E-5	0.0	0.0	4.020490832886116	4.745005762809499E-5
34	9.490011525618998E-5	0.0	0.0	4.153350994244782	4.745005762809499E-5
35	9.490011525618998E-5	0.0	0.0	4.305143728597058	4.745005762809499E-5
36	9.490011525618998E-5	0.0	0.0	4.444694348081286	4.745005762809499E-5
37	9.490011525618998E-5	0.0	0.0	4.59852743491157	4.745005762809499E-5
38	9.490011525618998E-5	0.0	0.0	4.754733024623258	4.745005762809499E-5
39	9.490011525618998E-5	0.0	0.0	4.99829417042827	4.745005762809499E-5
40	9.490011525618998E-5	0.0	0.0	5.1797906408557335	4.745005762809499E-5
41	9.490011525618998E-5	0.0	0.0	5.353362951659305	4.745005762809499E-5
42	9.490011525618998E-5	0.0	0.0	5.527314862923901	4.745005762809499E-5
43	9.490011525618998E-5	0.0	0.0	5.680958149523673	4.745005762809499E-5
44	9.490011525618998E-5	0.0	0.0	5.8369739390048485	4.745005762809499E-5
45	9.490011525618998E-5	0.0	0.0	6.009170198137205	4.745005762809499E-5
46	9.490011525618998E-5	0.0	0.0	6.198021427497023	4.745005762809499E-5
47	1.4235017288428498E-4	0.0	0.0	6.393325864694263	4.745005762809499E-5
48	1.4235017288428498E-4	0.0	0.0	6.575107035467495	4.745005762809499E-5
49	1.4235017288428498E-4	0.0	0.0	6.762202612695073	4.745005762809499E-5
50	1.4235017288428498E-4	0.0	0.0	6.958645851275387	4.745005762809499E-5
51	1.4235017288428498E-4	0.0	0.0	7.158030993428642	4.745005762809499E-5
52	1.4235017288428498E-4	0.0	0.0	7.395423631742001	4.745005762809499E-5
53	1.4235017288428498E-4	0.0	0.0	7.599743579888578	4.745005762809499E-5
54	1.4235017288428498E-4	0.0	0.0	7.8048701790148325	4.745005762809499E-5
55	1.4235017288428498E-4	0.0	0.0	8.01265398136826	4.745005762809499E-5
56	1.4235017288428498E-4	0.0	0.0	8.22689099155911	4.745005762809499E-5
57	1.4235017288428498E-4	0.0	0.0	8.451472114312883	4.745005762809499E-5
58	1.4235017288428498E-4	0.0	0.0	8.655744612401831	4.745005762809499E-5
59	1.4235017288428498E-4	0.0	0.0	8.872449025589342	4.745005762809499E-5
60	1.4235017288428498E-4	0.0	0.0	9.108655412461998	4.745005762809499E-5
61	1.4235017288428498E-4	0.0	0.0	9.347091952043176	4.745005762809499E-5
62	1.4235017288428498E-4	0.0	0.0	9.642610910950951	4.745005762809499E-5
63	1.4235017288428498E-4	0.0	0.0	9.915211492024357	4.745005762809499E-5
64	1.4235017288428498E-4	0.0	0.0	10.185059969755333	4.745005762809499E-5
65	1.4235017288428498E-4	0.0	0.0	10.427577214292526	4.745005762809499E-5
66	1.4235017288428498E-4	0.0	0.0	10.664969852605886	4.745005762809499E-5
67	1.4235017288428498E-4	0.0	0.0	10.912848953655054	4.745005762809499E-5
68	1.4235017288428498E-4	0.0	0.0	11.207039310949243	4.745005762809499E-5
69	1.4235017288428498E-4	0.0	0.0	11.58896482479778	4.745005762809499E-5
70	1.4235017288428498E-4	0.0	0.0	11.861328155583045	4.745005762809499E-5
71	1.4235017288428498E-4	0.0	0.0	12.113477761818741	4.745005762809499E-5
72	1.4235017288428498E-4	0.0	0.0	12.383421139664975	4.745005762809499E-5
73	1.4235017288428498E-4	0.0	0.0	12.651941015782363	4.745005762809499E-5
74	1.4235017288428498E-4	0.0	0.0	12.961125591287031	4.745005762809499E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	80	0.0	80.0	1
CTACGCG	85	0.0	75.29412	1
CGTTAGG	455	0.0	73.84616	2
TTAGGCG	65	0.0	73.84615	1
TCGTAAG	95	0.0	71.57895	1
CGTTTTT	6860	0.0	71.195335	1
GTTAGCG	90	0.0	66.666664	1
ACGGGAT	1295	0.0	65.791504	5
TACGGGA	940	0.0	65.53191	4
AGGGATT	4565	0.0	65.454544	6
TACGCGG	190	0.0	65.26316	2
CGTAAGG	365	0.0	64.65754	2
GGGCGAT	1480	0.0	64.324326	7
CGAGGGA	1395	0.0	64.22939	4
GCTAACG	50	3.0013325E-10	64.0	1
TGTAGGG	3070	0.0	63.061886	3
GCGAGGG	1755	0.0	62.905987	3
CTCGCGG	70	0.0	62.857143	1
GCGTAAG	140	0.0	62.857143	1
ATTACCG	45	9.427822E-9	62.22222	1
>>END_MODULE