FastQCFastQC Report
Thu 26 May 2016
SRR1547775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547775_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1817953
Sequences flagged as poor quality0
Sequence length86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160030.8802757827072537No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCC58080.31948020658399856No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATG22870.12580083203471157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20780.11430438520687827No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19070.10489820143865106No Hit
GAATCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTT18980.10440313913506014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGCG251.2851069E-680.01
CGTTTTT78100.073.341871
TACGGGA8700.070.8045964
TCTATCG750.069.333331
TACGTCG351.1818702E-768.571431
CGCGAGG1700.068.235292
GGGCGAT13900.067.0503547
AATCGCG303.7964728E-666.6666641
GGATCGC1150.066.086968
CGAGGGA13500.065.481484
ACGGGTA1800.064.444445
TTTGCGG5100.064.313722
ATCCGCG251.2357194E-464.01
AGGGATT41200.063.7864076
TCGATTG700.062.8571431
TTAGGGA28250.062.7256624
TACGAAG1150.062.6086961
TTGCGAG2750.062.5454561
GGGACCG6600.061.8181847
AGTAGGG34400.061.6279033
Produced by FastQC (version 0.11.5)