##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547775_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1817953
Sequences flagged as poor quality	0
Sequence length	86
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28717629113624	33.0	31.0	34.0	31.0	34.0
2	32.80487174310887	34.0	31.0	34.0	31.0	34.0
3	32.92839968910087	34.0	31.0	34.0	31.0	34.0
4	36.39721543956307	37.0	37.0	37.0	35.0	37.0
5	36.37268455235091	37.0	37.0	37.0	35.0	37.0
6	36.44441798000278	37.0	37.0	37.0	35.0	37.0
7	36.48039635788164	37.0	37.0	37.0	35.0	37.0
8	36.43104249669821	37.0	37.0	37.0	35.0	37.0
9	38.2928667572814	39.0	39.0	39.0	37.0	39.0
10-11	38.07057085634227	39.0	38.5	39.0	36.0	39.0
12-13	37.59293034528395	39.0	37.0	39.0	35.0	39.0
14-15	38.881218601360985	41.0	38.0	41.0	35.0	41.0
16-17	38.97447211231534	41.0	38.0	41.0	35.0	41.0
18-19	38.83244066265739	40.0	37.5	41.0	35.0	41.0
20-21	38.58185910196798	40.0	36.0	41.0	35.0	41.0
22-23	38.38264245555303	40.0	35.5	41.0	35.0	41.0
24-25	38.379195721781585	40.0	35.0	41.0	35.0	41.0
26-27	38.33682581452876	40.0	35.0	41.0	35.0	41.0
28-29	38.29025392845689	40.0	36.0	41.0	35.0	41.0
30-31	38.144356592277134	40.0	35.5	41.0	35.0	41.0
32-33	37.6820789646377	40.0	35.0	41.0	34.0	41.0
34-35	37.27515122778202	40.0	35.0	41.0	33.5	41.0
36-37	37.04910880534315	40.0	35.0	41.0	33.0	41.0
38-39	36.922292545516854	40.0	35.0	41.0	33.0	41.0
40-41	36.809668896830665	40.0	35.0	41.0	32.0	41.0
42-43	36.729302957777236	40.0	35.0	41.0	32.0	41.0
44-45	36.67339859721346	40.0	35.0	41.0	32.0	41.0
46-47	36.55830211232084	40.0	35.0	41.0	32.0	41.0
48-49	36.48396080646749	39.0	35.0	41.0	32.0	41.0
50-51	36.300093291740765	39.0	35.0	41.0	31.0	41.0
52-53	36.0831437886458	39.0	35.0	41.0	31.0	41.0
54-55	35.93595213957677	38.5	35.0	40.5	31.0	41.0
56-57	35.713682091891265	38.0	35.0	40.0	30.0	41.0
58-59	35.491586416150476	37.0	35.0	40.0	29.5	41.0
60-61	35.16533238208029	37.0	35.0	40.0	29.0	41.0
62-63	34.74105106127607	36.0	34.5	40.0	27.5	41.0
64-65	34.41397274847039	36.0	34.0	39.0	27.0	41.0
66-67	34.16965812647521	35.0	34.0	39.0	26.0	40.5
68-69	33.688071418788056	35.0	34.0	38.5	24.5	40.0
70-71	33.38513949480542	35.0	34.0	37.0	24.5	40.0
72-73	33.05836454517801	35.0	34.0	37.0	24.0	39.0
74-75	32.74477447986829	35.0	34.0	36.5	23.5	39.0
76-77	30.806102247967907	33.5	31.0	35.0	18.5	36.5
78-79	31.774755452973757	35.0	32.5	35.0	19.5	37.0
80-81	31.87838299449986	35.0	33.0	35.0	19.0	37.0
82-83	31.658734851781098	35.0	33.0	35.0	18.0	36.5
84-85	31.419958051720812	35.0	33.0	35.0	16.0	36.0
86	30.983749304850015	35.0	32.0	35.0	15.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	11.0
10	12.0
11	16.0
12	22.0
13	44.0
14	169.0
15	478.0
16	1444.0
17	4066.0
18	10179.0
19	17481.0
20	21058.0
21	21508.0
22	17117.0
23	12451.0
24	10360.0
25	9416.0
26	9986.0
27	11592.0
28	14256.0
29	17569.0
30	22518.0
31	28938.0
32	36207.0
33	49222.0
34	154234.0
35	170429.0
36	143416.0
37	279778.0
38	478283.0
39	274566.0
40	1122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.15867131878547	24.085716187382182	30.91768599078194	13.83792650305041
2	29.51148902089328	27.836363206309517	29.89582238924769	12.75632538354952
3	26.71972267709891	27.42837686122799	33.25223479374879	12.59966566792431
4	25.70770531471386	26.233406474204777	34.4936310234643	13.56525718761706
5	25.970363370230142	30.49072225739609	30.208096688968304	13.330817683405458
6	21.489499453506227	41.39793493011095	26.74326563998079	10.36929997640203
7	86.01812038044989	3.299865288046501	9.232251878898959	1.4497624526046602
8	86.89581083779395	5.1520033796253255	6.6299843835346675	1.32220139904607
9	82.04442029029353	6.277224988764836	7.7005291115886925	3.977825609352937
10-11	36.22062836607987	30.722796463934987	22.209457560233954	10.847117609751187
12-13	27.400515854920343	26.70924385833957	29.38747591384376	16.50276437289633
14-15	15.705686560653659	33.23548518581064	34.15984901699879	16.898979236536917
16-17	20.086245353977798	29.406178267534973	31.85956952682495	18.648006851662284
18-19	21.59998085759093	29.79840512928552	30.56030601451193	18.041307998611625
20-21	24.5408709686114	30.25950065815783	30.08647638305281	15.113151990177965
22-23	20.234021451599684	26.70454076645546	33.12753410016651	19.93390368177835
24-25	17.470171121035584	31.743174878558467	33.64448915896065	17.142164841445297
26-27	17.41142372767613	35.36925872121006	30.48874200818173	16.730575542932076
28-29	15.393247240165175	31.95077100453092	35.43017888801306	17.22580286729085
30-31	20.06039210034583	32.24813842822119	30.559755945285715	17.131713526147266
32-33	23.883565746749227	31.100611511958782	28.682919745449965	16.332902995842026
34-35	19.005331821009676	31.254438371069	30.10715898595838	19.633070821962946
36-37	20.545993213245886	32.83379713336924	29.338849794246606	17.281359859138274
38-39	18.98376910734216	33.09739030657008	28.342701929037766	19.57613865704999
40-41	19.991275902072275	29.994229773817032	28.819474430857124	21.195019893253566
42-43	19.918639260750965	29.857730095332496	29.18601305974357	21.037617584172967
44-45	18.785771689367106	34.03171039075268	28.170475254310755	19.01204266556946
46-47	19.76230958666148	33.377348039250734	28.15592592327744	18.704416450810335
48-49	20.56565818808297	30.69031487612716	29.942743294243577	18.801283641546288
50-51	18.657578056198375	33.5147003250359	28.200399020216693	19.62732259854903
52-53	20.74401813468225	30.412282385738244	29.500405125985107	19.343294353594402
54-55	20.400169861377055	30.577055622450082	30.621446209005406	18.401328307167457
56-57	20.48493552913634	29.21962228946513	29.9412306038715	20.354211577527032
58-59	21.401570887696217	30.5013660969233	28.730775768130417	19.366287247250067
60-61	21.667831896644195	32.11403155087068	27.55841322630453	18.6597233261806
62-63	21.060170422447666	30.329111918734974	30.209746896646944	18.400970762170417
64-65	18.9403136384714	33.28991453574432	29.48800106493402	18.281770760850254
66-67	18.29898792763069	32.5959196964938	26.487483449792155	22.617608926083346
68-69	17.388458337481772	34.99303887394228	26.12905834199234	21.4894444465836
70-71	18.53491261875307	33.27995828274988	28.31772878616774	19.867400312329305
72-73	20.156434187242464	33.79339289849628	27.328539296670485	18.721633617590776
74-75	19.609610369464995	34.953406386193706	25.209700140762713	20.227283103578586
76-77	22.27274302470966	32.565940923665245	26.54884367197612	18.612472379648977
78-79	18.114302184929972	33.10825417378777	28.767822930515806	20.00962071076645
80-81	16.727165113729566	32.69498716413461	29.335824413502436	21.242023308633392
82-83	18.151184326547494	31.132185485543356	30.101273245237913	20.615356942671234
84-85	16.69080553787694	31.607967862755526	28.698926759932736	23.00229983943479
86	17.47245390832436	31.082651751722956	29.629478869915776	21.815415470036903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1590.0
1	1760.0
2	5653.5
3	5933.0
4	3008.5
5	4786.5
6	7249.5
7	8454.0
8	9137.5
9	9681.5
10	9143.5
11	8426.0
12	7685.0
13	7202.5
14	7142.0
15	6954.0
16	7050.0
17	6997.0
18	6944.0
19	7297.0
20	7523.5
21	7681.0
22	8051.0
23	9617.0
24	11406.5
25	13548.5
26	16492.5
27	21074.5
28	26062.0
29	27863.0
30	30521.0
31	37292.5
32	42787.5
33	46478.5
34	51445.0
35	57159.0
36	60216.0
37	60426.5
38	64350.0
39	68630.5
40	75277.0
41	85295.5
42	92569.0
43	95903.0
44	95013.5
45	94494.0
46	95351.5
47	95643.5
48	91608.5
49	79476.5
50	71184.0
51	63875.0
52	50130.0
53	41379.5
54	36191.0
55	31464.5
56	26673.5
57	23735.0
58	20281.5
59	15047.5
60	12126.5
61	9724.5
62	6885.5
63	4244.0
64	3180.0
65	2518.0
66	1523.0
67	1014.5
68	670.5
69	380.5
70	178.5
71	98.0
72	89.5
73	68.0
74	44.5
75	35.0
76	29.5
77	21.0
78	16.5
79	14.0
80	12.0
81	7.0
82	4.5
83	4.0
84	2.0
85	3.0
86	5.0
87	2.5
88	0.5
89	2.0
90	2.5
91	1.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1817953.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.176388120502367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4893339204827	13.3098687461742
2	7.276947458470498	2.4998334775838513
3	2.5532791880055212	1.3156834293955189
4	1.5014310366505874	1.031566488867148
5	1.0772482226786795	0.9251616787425178
6	0.8197780591365512	0.8448495669840926
7	0.6842202749866366	0.822670310216113
8	0.5404780926169204	0.7426769191533639
9	0.45447718229057066	0.7025648827441632
>10	4.417006219495124	17.74444822575029
>50	1.7746321345031766	22.098892166525065
>100	1.403407432255798	35.54662700068019
>500	0.004850486517007276	0.5633126366868658
>1k	0.0022635603746033956	0.640405709031706
>5k	3.233657678004851E-4	0.32259118456688196
>10k+	3.233657678004851E-4	0.8888475768980392
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16003	0.8802757827072537	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCC	5808	0.31948020658399856	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATG	2287	0.12580083203471157	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2078	0.11430438520687827	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1907	0.10489820143865106	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTT	1898	0.10440313913506014	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019747485221015066	0.0
2	0.0	0.0	0.0	0.09450189306324201	0.0
3	0.0	0.0	0.0	0.14004762499360546	0.0
4	0.0	0.0	0.0	0.24494582643225649	0.0
5	0.0	0.0	0.0	0.49346710283489176	0.0
6	0.0	0.0	0.0	0.6221282948459064	0.0
7	0.0	0.0	0.0	0.7485892099520725	0.0
8	0.0	0.0	0.0	0.960035820508011	0.0
9	0.0	0.0	0.0	1.0415010729100258	0.0
10	0.0	0.0	0.0	1.4956932329933723	0.0
11	0.0	0.0	0.0	1.7411891286518408	0.0
12	0.0	0.0	0.0	2.0501080060925667	0.0
13	0.0	0.0	0.0	2.1188116524464604	0.0
14	0.0	0.0	0.0	2.1530809652394756	0.0
15	0.0	0.0	0.0	2.217934127009884	0.0
16	0.0	0.0	0.0	2.309025590870611	0.0
17	0.0	0.0	0.0	2.4090831831185953	0.0
18	0.0	0.0	0.0	2.5144764468608374	0.0
19	0.0	0.0	0.0	2.6582645425926854	0.0
20	0.0	0.0	0.0	2.752656421810685	0.0
21	0.0	0.0	0.0	2.852383972522942	0.0
22	0.0	0.0	0.0	2.9809901576113353	0.0
23	0.0	0.0	0.0	3.0985949581754864	0.0
24	0.0	0.0	0.0	3.18864129050641	0.0
25	0.0	0.0	0.0	3.267521217545228	0.0
26	0.0	0.0	0.0	3.344035846911334	0.0
27	0.0	0.0	0.0	3.4391978230460305	0.0
28	0.0	0.0	0.0	3.5311143907460756	0.0
29	0.0	0.0	0.0	3.6428334505897566	0.0
30	0.0	0.0	0.0	3.756807794260908	0.0
31	5.500692262121188E-5	0.0	0.0	3.8584605872649074	0.0
32	5.500692262121188E-5	0.0	0.0	3.9571430064473616	0.0
33	5.500692262121188E-5	0.0	0.0	4.0569805710048605	0.0
34	5.500692262121188E-5	0.0	0.0	4.1736502538844515	0.0
35	5.500692262121188E-5	0.0	0.0	4.301156300520421	0.0
36	5.500692262121188E-5	0.0	0.0	4.4173859280190415	0.0
37	5.500692262121188E-5	0.0	0.0	4.543296773898995	0.0
38	5.500692262121188E-5	0.0	0.0	4.678998852005525	0.0
39	5.500692262121188E-5	0.0	0.0	4.870862998108312	0.0
40	5.500692262121188E-5	0.0	0.0	5.0152561699889935	0.0
41	5.500692262121188E-5	0.0	0.0	5.163829867988887	0.0
42	5.500692262121188E-5	0.0	0.0	5.319994521310507	0.0
43	5.500692262121188E-5	0.0	0.0	5.4621324093637185	0.0
44	5.500692262121188E-5	0.0	0.0	5.612246301197006	0.0
45	5.500692262121188E-5	0.0	0.0	5.768906016822217	0.0
46	5.500692262121188E-5	0.0	0.0	5.93695216543002	0.0
47	5.500692262121188E-5	0.0	0.0	6.10444824481161	0.0
48	5.500692262121188E-5	0.0	0.0	6.27650989877076	0.0
49	5.500692262121188E-5	0.0	0.0	6.445986227366714	0.0
50	5.500692262121188E-5	0.0	0.0	6.628609210469137	0.0
51	5.500692262121188E-5	0.0	0.0	6.81299241509544	0.0
52	5.500692262121188E-5	0.0	0.0	7.023393894121575	0.0
53	5.500692262121188E-5	0.0	0.0	7.226699480129574	0.0
54	5.500692262121188E-5	0.0	0.0	7.417518494702558	0.0
55	5.500692262121188E-5	0.0	0.0	7.611142862329224	0.0
56	5.500692262121188E-5	0.0	0.0	7.819014022914784	0.0
57	5.500692262121188E-5	0.0	0.0	8.022209595077541	0.0
58	5.500692262121188E-5	0.0	0.0	8.234976371776389	0.0
59	5.500692262121188E-5	0.0	0.0	8.461384865285297	0.0
60	5.500692262121188E-5	0.0	0.0	8.685923123425082	0.0
61	5.500692262121188E-5	0.0	0.0	8.93989008516722	0.0
62	5.500692262121188E-5	0.0	0.0	9.221690549755687	0.0
63	5.500692262121188E-5	0.0	0.0	9.487649020629247	0.0
64	5.500692262121188E-5	0.0	0.0	9.74431132157982	0.0
65	5.500692262121188E-5	0.0	0.0	9.99569295795876	0.0
66	5.500692262121188E-5	0.0	0.0	10.23909859055762	0.0
67	5.500692262121188E-5	0.0	0.0	10.49411068382956	0.0
68	5.500692262121188E-5	0.0	0.0	10.778551480703847	0.0
69	5.500692262121188E-5	0.0	0.0	11.141927211539572	0.0
70	5.500692262121188E-5	0.0	0.0	11.419052087705237	0.0
71	5.500692262121188E-5	0.0	0.0	11.692106451596933	0.0
72	5.500692262121188E-5	0.0	0.0	11.968681258536387	0.0
73	5.500692262121188E-5	0.0	0.0	12.247621363148552	0.0
74	5.500692262121188E-5	0.0	0.0	12.54212842686252	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGCG	25	1.2851069E-6	80.0	1
CGTTTTT	7810	0.0	73.34187	1
TACGGGA	870	0.0	70.804596	4
TCTATCG	75	0.0	69.33333	1
TACGTCG	35	1.1818702E-7	68.57143	1
CGCGAGG	170	0.0	68.23529	2
GGGCGAT	1390	0.0	67.050354	7
AATCGCG	30	3.7964728E-6	66.666664	1
GGATCGC	115	0.0	66.08696	8
CGAGGGA	1350	0.0	65.48148	4
ACGGGTA	180	0.0	64.44444	5
TTTGCGG	510	0.0	64.31372	2
ATCCGCG	25	1.2357194E-4	64.0	1
AGGGATT	4120	0.0	63.786407	6
TCGATTG	70	0.0	62.857143	1
TTAGGGA	2825	0.0	62.725662	4
TACGAAG	115	0.0	62.608696	1
TTGCGAG	275	0.0	62.545456	1
GGGACCG	660	0.0	61.818184	7
AGTAGGG	3440	0.0	61.627903	3
>>END_MODULE