FastQCFastQC Report
Thu 26 May 2016
SRR1547774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547774_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1215686
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74000.6087098148699582No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35430.2914403883897651No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20330.16723068292305743No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA13280.10923873434423034No Hit
GTGGAGGGGAATTTTGTTTTGCTTTGTTTTGATCTTCATCTTTGTATTCA12450.10241131344771594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTCG500.080.080
GGTACCG404.1836756E-1180.08
AGCGAAT204.090996E-580.04
GGCGATA2000.080.08
ATCGACG251.2848504E-680.01
CGTAAGG1450.077.241392
GACGTAG1150.076.521741
TGATGCG900.075.555551
CGCATGG600.073.333332
CTATGCG550.072.727271
CGTATGG1100.072.727272
TAGCACG1000.072.01
GTTAGCG451.1823431E-1071.111111
CACGAAC451.1823431E-1071.1111180
ATGGGAC10500.070.857145
AAGTGCG2550.070.588231
TAGGGTA3200.070.05
ATGACGG800.070.02
CGTTTTT46700.069.721631
TCGATGG1550.069.6774142
Produced by FastQC (version 0.11.5)