##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547774_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1215686
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.819825185121815	31.0	31.0	33.0	30.0	34.0
2	32.25106318572394	33.0	31.0	34.0	31.0	34.0
3	32.39396439541132	34.0	31.0	34.0	31.0	34.0
4	35.96659992794192	37.0	35.0	37.0	35.0	37.0
5	35.91066278627869	37.0	35.0	37.0	35.0	37.0
6	35.97361242952539	37.0	35.0	37.0	35.0	37.0
7	36.23231492342595	37.0	35.0	37.0	35.0	37.0
8	36.185679525798605	37.0	36.0	37.0	35.0	37.0
9	38.08733998746387	39.0	39.0	39.0	37.0	39.0
10-11	37.54890243039732	39.0	37.5	39.0	35.0	39.0
12-13	36.109329629526044	37.0	35.0	39.0	32.5	39.0
14-15	37.00643628371142	38.0	35.0	41.0	33.0	41.0
16-17	37.31361675630056	38.5	35.0	41.0	33.5	41.0
18-19	37.193623188882654	38.0	35.0	41.0	33.0	41.0
20-21	36.92639341079851	38.0	35.0	41.0	33.0	41.0
22-23	36.68714084064471	37.0	35.0	41.0	33.0	41.0
24-25	36.616227381083604	37.0	35.0	41.0	33.0	41.0
26-27	36.53939339599206	37.0	35.0	41.0	33.0	41.0
28-29	36.607722717872875	37.0	35.0	41.0	33.0	41.0
30-31	36.42212709531903	36.0	35.0	41.0	32.5	41.0
32-33	35.978841164577034	36.0	35.0	40.5	31.0	41.0
34-35	35.69496605208911	36.0	35.0	40.0	30.5	41.0
36-37	35.42935305662811	36.0	35.0	40.0	30.0	41.0
38-39	35.41659729568326	36.0	35.0	40.0	30.0	41.0
40-41	35.24937607243976	36.0	35.0	40.0	29.0	41.0
42-43	35.153393228185564	35.5	35.0	40.0	29.0	41.0
44-45	34.99018619939689	35.0	34.5	40.0	28.5	41.0
46-47	34.87641915757852	35.0	34.0	40.0	28.0	41.0
48-49	34.850924909886274	35.5	34.0	40.0	28.0	41.0
50-51	34.6880082521309	35.0	34.0	40.0	28.0	41.0
52-53	34.48308773811659	35.0	34.0	40.0	27.0	41.0
54-55	34.29228517890311	35.0	34.0	39.0	26.5	41.0
56-57	33.95874757133009	35.0	34.0	39.0	25.5	41.0
58-59	33.68409893673201	35.0	34.0	38.5	25.0	40.5
60-61	33.377828238541866	35.0	33.0	38.0	24.5	40.0
62-63	32.89861814646216	35.0	32.5	37.0	22.5	40.0
64-65	32.68429635613143	35.0	32.5	37.0	23.0	40.0
66-67	32.52067639176563	35.0	33.0	36.0	22.0	39.5
68-69	32.3140704096288	35.0	33.0	36.0	21.0	39.0
70-71	32.11957281732289	35.0	33.0	35.0	20.0	38.5
72-73	31.9085602696749	35.0	32.5	35.0	20.0	37.5
74-75	31.6486563964708	35.0	32.0	35.0	19.5	37.0
76-77	29.61277624320754	32.5	28.5	34.5	16.0	35.5
78-79	30.537023129327807	34.0	30.5	35.0	17.0	36.0
80-81	31.08895882653909	34.5	31.5	35.0	18.5	36.0
82-83	31.028186554751805	34.5	31.5	35.0	18.0	36.0
84-85	30.939803534794347	34.5	31.5	35.0	18.0	35.5
86	30.564431933903986	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	6.0
12	17.0
13	70.0
14	241.0
15	805.0
16	1935.0
17	4279.0
18	7985.0
19	11313.0
20	13131.0
21	12986.0
22	10787.0
23	9476.0
24	9729.0
25	11011.0
26	13002.0
27	15911.0
28	19208.0
29	23811.0
30	29253.0
31	36577.0
32	45908.0
33	65169.0
34	199984.0
35	173460.0
36	85881.0
37	148899.0
38	181608.0
39	82827.0
40	409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.623215205242143	24.106553830512155	29.852280934386016	14.417950029859682
2	29.922940627760784	25.76643968919606	30.92393924088951	13.386680442153647
3	29.604848620449687	24.328897429105872	32.88974291058711	13.176511039857331
4	25.576834807672377	28.142875709681615	32.41725248131508	13.863037001330936
5	23.444129487384078	33.11899618816043	29.903034171652877	13.533840152802615
6	22.250317927491146	39.96171708812967	27.107328701654872	10.680636282724322
7	86.65823247121378	4.485451012843777	7.161964520443602	1.6943519954988377
8	87.42315038587267	3.784200854496967	6.857856387257893	1.9347923723724711
9	84.08503511597567	5.9794223179340715	7.741061425400968	2.19448114068929
10-11	50.76179210750145	23.34200607722718	16.779785240596667	9.11641657467471
12-13	33.6645729242584	32.14086532213088	22.03352674950604	12.161035004104678
14-15	11.347790465630105	37.385023764360206	37.64351979047221	13.623665979537481
16-17	15.577953517602408	22.264589704907355	37.79195450140908	24.36550227608116
18-19	23.94236669666345	26.129897029331588	30.21964553346835	19.70809074053662
20-21	26.637059240626282	27.59643526371119	28.18034426652935	17.586161229133182
22-23	19.58910442334616	27.58113526025635	27.597915909206815	25.231844407190675
24-25	16.15170364715889	26.5117801800794	39.02113703703095	18.315379135730772
26-27	14.371803245245896	39.82430496032693	29.173980781221466	16.62991101320571
28-29	13.008046485687915	29.170279167482395	40.38156234422375	17.440112002605936
30-31	23.39510366986212	32.021755617815785	28.664967763057238	15.918172949264859
32-33	28.099155538518993	30.11974309155489	25.73781387628055	16.043287493645565
34-35	18.165875069713724	30.36137621063334	28.394749960104832	23.07799875954811
36-37	24.279830482542366	30.040117267123257	29.83628173722491	15.84377051310947
38-39	17.450312004909165	34.17975529865443	27.876606294717547	20.493326401718864
40-41	20.37672556893803	26.73177942330503	27.930115177767945	24.961379829988996
42-43	21.611583912293142	25.257303283907195	27.299236809505086	25.831875994294577
44-45	17.06431595000683	37.252670508667535	25.48807833601769	20.19493520530795
46-47	20.756305493359307	33.681518089375054	26.073097822957575	19.489078594308072
48-49	20.89437568582677	25.67126708706031	32.47619039784945	20.958166829263476
50-51	18.441974325607106	34.72866348711756	26.518114052477365	20.311248134797964
52-53	23.598898070718917	25.957936506630823	29.619572817322894	20.823592605327363
54-55	24.0309997811935	28.273830577961746	30.027243877119584	17.667925763725172
56-57	24.50065230659891	22.90365275243772	28.775933917146368	23.819761023817005
58-59	25.800412277512454	26.835918156497645	27.152282744063843	20.211386821926055
60-61	26.102998636160983	30.870841648254565	24.744341877754618	18.281817837829834
62-63	24.86756448622424	25.14189519333117	31.66348053691496	18.327059783529627
64-65	17.45693378059795	33.110523605602104	31.10239815215442	18.330144461645524
66-67	15.68776805852827	29.380407440737166	23.052375366665405	31.87944913406916
68-69	15.716599516651502	35.07443533938863	21.32569594451199	27.883269199447884
70-71	18.713096967473508	31.455902264235995	27.77349578756356	22.05750498072693
72-73	25.020070972274087	30.767690012059035	25.279677482507818	18.932561533159056
74-75	24.219453049553916	33.920683465960785	20.382442505712824	21.47742097877248
76-77	31.96697173447749	27.927236144859773	23.337605269781836	16.7681868508809
78-79	19.050231721020065	30.40962057636594	30.804253729992777	19.73589397262122
80-81	14.747928330177364	29.056968658025177	33.58906000398129	22.60604300781616
82-83	18.694712285902774	25.03191613623913	34.15014238874183	22.12322918911627
84-85	14.405323414105286	25.981297802228536	31.17412720060937	28.43925158305681
86	18.330391235894794	24.854444321971297	33.344547851994676	23.47061659013923
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2446.0
1	2273.0
2	3438.0
3	3163.5
4	1773.5
5	2499.5
6	3145.0
7	3287.0
8	3658.5
9	3662.5
10	3044.0
11	2691.0
12	2646.0
13	2671.5
14	2640.0
15	2842.0
16	3127.0
17	3425.0
18	3543.5
19	3870.0
20	4117.5
21	3942.0
22	3813.5
23	4263.5
24	5307.0
25	5667.5
26	6028.0
27	8787.0
28	12033.5
29	12986.0
30	15104.5
31	18357.5
32	19145.0
33	19082.5
34	19386.0
35	20910.5
36	22416.0
37	25049.5
38	29467.5
39	36312.5
40	45057.0
41	55852.0
42	68213.5
43	73464.0
44	77653.0
45	82540.5
46	85431.0
47	91377.0
48	86388.0
49	65937.5
50	54230.0
51	45388.5
52	31125.5
53	24587.0
54	23501.0
55	24015.5
56	23868.5
57	23238.0
58	21348.0
59	15816.0
60	10965.0
61	8855.0
62	6889.5
63	4618.5
64	3412.0
65	3358.0
66	2544.5
67	1659.5
68	1141.5
69	638.5
70	482.5
71	437.0
72	317.5
73	200.5
74	126.5
75	73.5
76	76.5
77	49.0
78	25.5
79	9.0
80	10.0
81	8.5
82	6.0
83	4.5
84	2.0
85	1.5
86	2.0
87	1.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1215686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.322846084504837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.81339286086481	12.536140264581586
2	8.68342904071663	2.6610969335324084
3	2.476185260822248	1.1382661688489633
4	1.112459529289691	0.6818418457018656
5	0.6258783399193186	0.479511873510456
6	0.4065282366709663	0.37375017597086235
7	0.295235102476039	0.3166689423788351
8	0.23329967215061914	0.28598519743435014
9	0.17516404570445857	0.24156105406817308
>10	1.6142330761029076	5.278302830388074
>50	0.6190181501999342	7.034479662626918
>100	1.919226579222503	65.39240431765222
>500	0.02108446101117679	1.9082072968735475
>1k	0.004325017643318316	1.0587713245643937
>5k	5.406272054147895E-4	0.6130121118673432
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7400	0.6087098148699582	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3543	0.2914403883897651	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2033	0.16723068292305743	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	1328	0.10923873434423034	No Hit
GTGGAGGGGAATTTTGTTTTGCTTTGTTTTGATCTTCATCTTTGTATTCA	1245	0.10241131344771594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.22580830905349E-5	0.0	0.0	0.061858078484082236	0.0
2	8.22580830905349E-5	0.0	0.0	0.20260165865198743	0.0
3	8.22580830905349E-5	0.0	0.0	0.2777032885136458	0.0
4	8.22580830905349E-5	0.0	0.0	0.42379364408243575	0.0
5	8.22580830905349E-5	0.0	0.0	0.6445743390974314	0.0
6	8.22580830905349E-5	0.0	0.0	0.8625582592873489	0.0
7	8.22580830905349E-5	0.0	0.0	0.9774728013648261	0.0
8	8.22580830905349E-5	0.0	0.0	1.237655118180188	0.0
9	8.22580830905349E-5	0.0	0.0	1.3273986868319616	0.0
10	8.22580830905349E-5	0.0	0.0	1.4905987236835827	0.0
11	1.645161661810698E-4	0.0	0.0	1.8075391178314137	0.0
12	1.645161661810698E-4	0.0	0.0	2.021903682365348	0.0
13	1.645161661810698E-4	0.0	0.0	2.095606924814467	0.0
14	1.645161661810698E-4	0.0	0.0	2.1297440292970387	0.0
15	1.645161661810698E-4	0.0	0.0	2.1814843635609855	0.0
16	1.645161661810698E-4	0.0	0.0	2.299277938546631	0.0
17	1.645161661810698E-4	0.0	0.0	2.424145708678063	0.0
18	1.645161661810698E-4	0.0	0.0	2.587263487446594	0.0
19	1.645161661810698E-4	0.0	0.0	2.6766780237660055	0.0
20	1.645161661810698E-4	0.0	0.0	2.7696296576583097	0.0
21	1.645161661810698E-4	0.0	0.0	2.8853667805666925	0.0
22	1.645161661810698E-4	0.0	0.0	2.9919732562520256	0.0
23	1.645161661810698E-4	0.0	0.0	3.1249845766094206	0.0
24	1.645161661810698E-4	0.0	0.0	3.2114378219375728	0.0
25	1.645161661810698E-4	0.0	0.0	3.292215259532478	0.0
26	1.645161661810698E-4	0.0	0.0	3.3651781792337823	0.0
27	1.645161661810698E-4	0.0	0.0	3.4386346474336302	0.0
28	1.645161661810698E-4	0.0	0.0	3.519658859277807	0.0
29	1.645161661810698E-4	0.0	0.0	3.6149959775797367	0.0
30	1.645161661810698E-4	0.0	0.0	3.735010520808827	0.0
31	1.645161661810698E-4	0.0	0.0	3.8540379670408313	0.0
32	1.645161661810698E-4	0.0	0.0	3.944768632689691	0.0
33	1.645161661810698E-4	0.0	0.0	4.043478332398333	0.0
34	1.645161661810698E-4	0.0	0.0	4.1418589997746125	0.0
35	1.645161661810698E-4	0.0	0.0	4.292309033747201	0.0
36	1.645161661810698E-4	0.0	0.0	4.398997767515625	0.0
37	1.645161661810698E-4	0.0	0.0	4.516215535919637	0.0
38	1.645161661810698E-4	0.0	0.0	4.622904269688061	0.0
39	1.645161661810698E-4	0.0	0.0	4.744975264994415	0.0
40	1.645161661810698E-4	0.0	0.0	4.8747785201112785	0.0
41	1.645161661810698E-4	0.0	0.0	5.014535003282098	0.0
42	1.645161661810698E-4	0.0	0.0	5.1370995470869945	0.0
43	2.4677424927160467E-4	0.0	0.0	5.246667313763587	0.0
44	2.4677424927160467E-4	0.0	0.0	5.389220571759484	0.0
45	2.4677424927160467E-4	0.0	0.0	5.528977054930302	0.0
46	2.4677424927160467E-4	0.0	0.0	5.683375476891237	0.0
47	2.4677424927160467E-4	0.0	0.0	5.832920671949829	0.0
48	2.4677424927160467E-4	0.0	0.0	5.984193286753323	0.0
49	2.4677424927160467E-4	0.0	0.0	6.153644937919824	0.0
50	2.4677424927160467E-4	0.0	0.0	6.3287723968195735	0.0
51	2.4677424927160467E-4	0.0	0.0	6.482430496032693	0.0
52	2.4677424927160467E-4	0.0	0.0	6.6579692453478945	0.0
53	2.4677424927160467E-4	0.0	0.0	6.818537023540618	0.0
54	2.4677424927160467E-4	0.0	0.0	7.00649674340249	0.0
55	2.4677424927160467E-4	0.0	0.0	7.197993560837256	0.0
56	2.4677424927160467E-4	0.0	0.0	7.375671020312811	0.0
57	2.4677424927160467E-4	0.0	0.0	7.543230735568231	0.0
58	2.4677424927160467E-4	0.0	0.0	7.6918710917128275	0.0
59	2.4677424927160467E-4	0.0	0.0	7.873414681093638	0.0
60	2.4677424927160467E-4	0.0	0.0	8.06129214287242	0.0
61	2.4677424927160467E-4	0.0	0.0	8.25196637947628	0.0
62	2.4677424927160467E-4	0.0	0.0	8.462958362603501	0.0
63	2.4677424927160467E-4	0.0	0.0	8.726019712327032	0.0
64	2.4677424927160467E-4	0.0	0.0	8.961771378464505	0.0
65	2.4677424927160467E-4	0.0	0.0	9.178521427408064	0.0
66	2.4677424927160467E-4	0.0	0.0	9.384577925549854	0.0
67	2.4677424927160467E-4	0.0	0.0	9.598695715834516	0.0
68	2.4677424927160467E-4	0.0	0.0	9.830498993983644	0.0
69	2.4677424927160467E-4	0.0	0.0	10.085005503065759	0.0
70	2.4677424927160467E-4	0.0	0.0	10.284234580311034	0.0
71	2.4677424927160467E-4	0.0	0.0	10.496213660435343	0.0
72	3.290323323621396E-4	0.0	0.0	10.705313707651483	0.0
73	3.290323323621396E-4	0.0	0.0	10.928808919408466	0.0
74	3.290323323621396E-4	0.0	0.0	11.182657363825856	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTCG	50	0.0	80.0	80
GGTACCG	40	4.1836756E-11	80.0	8
AGCGAAT	20	4.090996E-5	80.0	4
GGCGATA	200	0.0	80.0	8
ATCGACG	25	1.2848504E-6	80.0	1
CGTAAGG	145	0.0	77.24139	2
GACGTAG	115	0.0	76.52174	1
TGATGCG	90	0.0	75.55555	1
CGCATGG	60	0.0	73.33333	2
CTATGCG	55	0.0	72.72727	1
CGTATGG	110	0.0	72.72727	2
TAGCACG	100	0.0	72.0	1
GTTAGCG	45	1.1823431E-10	71.11111	1
CACGAAC	45	1.1823431E-10	71.11111	80
ATGGGAC	1050	0.0	70.85714	5
AAGTGCG	255	0.0	70.58823	1
TAGGGTA	320	0.0	70.0	5
ATGACGG	80	0.0	70.0	2
CGTTTTT	4670	0.0	69.72163	1
TCGATGG	155	0.0	69.677414	2
>>END_MODULE