FastQCFastQC Report
Thu 26 May 2016
SRR1547773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547773_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3906623
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271480.6949224432457393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150700.072.780361
TACGGGA14000.068.857144
GCGCGAC1050.068.5714349
TAGGGTA33850.066.2924655
TGTAGCG3150.063.4920651
CTACGGG7800.063.0769233
TTGCGGG28750.062.8869553
TAGGGAC46750.062.5454565
TAGCGGG24350.061.9301833
CGGGATC15050.061.926916
ACGTAGG5750.061.9130442
GTAGGGT32650.061.8683054
ATAGGGC14000.061.7142874
TTAGCGG11550.061.645022
ATAGGGA61450.061.6436124
GGGACCG16500.061.0909087
CGTTAGG7300.060.821922
TTAGGGA66450.060.5568124
ACGGGAT18950.060.3693925
TTACGGG16500.060.3636363
Produced by FastQC (version 0.11.5)