##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547773_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3906623
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.678029848285846	31.0	31.0	33.0	30.0	34.0
2	32.11769628141748	33.0	31.0	34.0	30.0	34.0
3	32.27582620590725	33.0	31.0	34.0	30.0	34.0
4	35.90561336479102	37.0	35.0	37.0	35.0	37.0
5	35.855677141101154	37.0	35.0	37.0	35.0	37.0
6	35.91925814187855	37.0	35.0	37.0	35.0	37.0
7	36.18129008097275	37.0	35.0	37.0	35.0	37.0
8	36.13088695786617	37.0	35.0	37.0	35.0	37.0
9	37.98740190696671	39.0	39.0	39.0	35.0	39.0
10-11	37.429756851377775	39.0	37.0	39.0	34.5	39.0
12-13	36.92090470465156	39.0	35.0	39.0	33.0	39.0
14-15	38.09024315886125	40.0	36.0	41.0	33.0	41.0
16-17	38.170101645334086	40.0	36.0	41.0	34.0	41.0
18-19	38.0282495905031	40.0	36.0	41.0	33.5	41.0
20-21	37.847654611156486	40.0	35.0	41.0	33.5	41.0
22-23	37.64576976073709	39.5	35.0	41.0	33.0	41.0
24-25	37.56390980138089	39.5	35.0	41.0	33.0	41.0
26-27	37.538728845859964	40.0	35.0	41.0	33.0	41.0
28-29	37.517980107115534	40.0	35.0	41.0	33.0	41.0
30-31	37.355852100394635	40.0	35.0	41.0	33.0	41.0
32-33	37.04078074592813	40.0	35.0	41.0	31.5	41.0
34-35	36.75942034846976	39.0	35.0	41.0	31.0	41.0
36-37	36.56176498218538	39.0	35.0	41.0	30.0	41.0
38-39	36.44450757086108	39.0	35.0	41.0	30.0	41.0
40-41	36.302061012798006	39.0	35.0	41.0	30.0	41.0
42-43	36.237337721095685	39.0	35.0	41.0	30.0	41.0
44-45	36.12115195144246	39.0	35.0	41.0	30.0	41.0
46-47	36.017028517980876	39.0	35.0	41.0	29.0	41.0
48-49	35.906173311322846	38.0	35.0	40.5	29.0	41.0
50-51	35.720089448098776	38.0	35.0	40.0	28.5	41.0
52-53	35.52248054137806	38.0	35.0	40.0	28.0	41.0
54-55	35.330224595513826	37.5	34.5	40.0	27.5	41.0
56-57	35.03593922423536	37.0	34.0	40.0	26.5	41.0
58-59	34.73427561349022	36.5	34.0	40.0	26.0	41.0
60-61	34.37936063961124	36.0	34.0	39.5	25.5	41.0
62-63	33.947620489614685	35.5	33.5	39.0	24.5	41.0
64-65	33.51921608509447	35.0	33.0	39.0	23.0	40.0
66-67	33.150788033552246	35.0	33.0	38.0	22.0	40.0
68-69	32.66438993473392	35.0	32.5	37.5	20.5	40.0
70-71	32.32681807279586	35.0	32.0	37.0	19.5	39.0
72-73	32.01310262085694	35.0	32.0	36.5	19.0	39.0
74-75	31.68953249392122	35.0	32.0	36.0	18.0	38.5
76-77	29.65802510249901	33.0	28.5	34.5	14.0	36.0
78-79	30.743986046260414	34.0	30.5	35.0	15.5	37.0
80-81	30.882756283368014	34.5	31.0	35.0	15.0	36.5
82-83	30.661376718459906	34.0	31.0	35.0	12.5	36.0
84-85	30.426867399285776	34.0	31.0	35.0	11.0	36.0
86	30.01733108108973	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	11.0
10	13.0
11	21.0
12	41.0
13	136.0
14	543.0
15	1682.0
16	4141.0
17	9283.0
18	18738.0
19	30828.0
20	35979.0
21	34240.0
22	30553.0
23	28999.0
24	30593.0
25	35777.0
26	43161.0
27	52725.0
28	64652.0
29	80552.0
30	99540.0
31	120197.0
32	142387.0
33	175538.0
34	358333.0
35	351530.0
36	348908.0
37	581947.0
38	825874.0
39	398340.0
40	1355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.896391589359915	24.73000338143711	27.446569581963757	14.927035447239215
2	31.04809447955434	26.17879943879919	29.131579883700066	13.64152619794641
3	28.264206707429924	24.98899945042048	33.00374773813598	13.74304610401362
4	26.19477231358132	27.07998698620266	32.51465524060039	14.210585459615633
5	22.729068046750353	30.86038760330854	31.658237818187217	14.752306531753895
6	21.194213006988388	40.06644613519144	27.629233739728658	11.110107118091507
7	86.9153486271903	3.6863295997591785	7.475714958930002	1.922606814120533
8	87.33747791890848	3.03901861019095	7.406806338876313	2.216697132024257
9	81.99636873074265	5.832428673050868	9.331051396564245	2.8401511996422486
10-11	33.99715048009496	29.00251700765597	23.78300900803584	13.21732350421323
12-13	27.296132746876268	25.30135362434512	29.26377846032238	18.138735168456236
14-15	17.499116244388055	30.894675529223065	33.36426371318655	18.241944513202323
16-17	21.630664131143444	26.99541266203573	31.952187349534366	19.42173585728646
18-19	22.589023819293544	28.998114228068587	30.069896685705277	18.34296526693259
20-21	24.862560323839798	28.28526069702656	29.849629718557434	17.002549260576206
22-23	21.171917535938327	25.109819913516095	32.04834456767392	21.66991798287165
24-25	19.463600659700205	27.98582048997305	33.679177642685254	18.871401207641487
26-27	18.716497599077258	32.40174442222861	29.29180778385834	19.589950194835794
28-29	17.094585272241524	28.827237744722233	33.97740196584109	20.100775017195158
30-31	20.968544955579283	28.64018360614782	30.181527626290023	20.20974381198288
32-33	22.44062198988743	28.708029927638268	29.197851955512473	19.653496126961826
34-35	19.090836254227757	28.911146532439915	30.143681640127546	21.854335573204786
36-37	20.802813580936782	30.072622825391647	29.620941155570936	19.50362243810063
38-39	19.37298531237849	30.70501043996311	28.941441239658904	20.980563007999493
40-41	19.952321992677565	28.32930129167826	29.317699711489947	22.400677004154225
42-43	20.32837568406268	27.918537314708892	28.895276047880742	22.857810953347688
44-45	19.453233649625265	30.443070652069576	28.46043756973734	21.64325812856782
46-47	20.32760775739046	29.779671598718384	28.42316752857903	21.469553115312127
48-49	20.430189962020904	27.65270669834279	30.484487497257863	21.432615842378443
50-51	19.13816613479212	29.778007757595244	29.458985420400175	21.62484068721246
52-53	20.857886722112678	27.4814974467718	30.503109719059147	21.15750611205637
54-55	20.62958212246229	27.8143808604004	30.584778720649524	20.971258296487786
56-57	21.287298518439073	26.968253655394953	29.959033671792746	21.78541415437323
58-59	21.37292234239137	28.733742672379698	28.999739672858116	20.893595312370813
60-61	21.842202331783742	30.54813581960686	27.34850534592153	20.26115650268787
62-63	21.511584301838184	28.68764147449088	29.618112625661603	20.18266159800933
64-65	19.190116374167662	31.491840907095465	29.042001237385843	20.27604148135103
66-67	18.167903583222646	30.64338432451762	27.502692222925017	23.686019869334718
68-69	17.969432934787925	32.71704231506342	26.636854899999307	22.67666985014935
70-71	18.810107860420626	30.89746566279879	28.363448431036215	21.92897804574437
72-73	20.455608334871318	31.204815514576147	27.379030431142194	20.960545719410344
74-75	19.92823469272566	32.23543710258195	25.731917310679837	22.104410894012553
76-77	22.544637657639345	30.40679891558515	26.371805009083293	20.676758417692213
78-79	18.391869397175004	30.781662320628328	28.69292736975132	22.13354091244535
80-81	16.990185641153495	30.526531482561793	29.11122982688629	23.37205304939842
82-83	18.562617892742658	29.44685217897913	29.615706455421986	22.374823472856225
84-85	17.174206469372653	29.446685794866816	28.57897985037205	24.800127885388477
86	17.989629406267255	29.205198454010024	29.373579175671672	23.43159296405105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1852.0
1	2500.5
2	9509.0
3	10183.5
4	4974.0
5	6487.0
6	8310.0
7	9096.0
8	9926.5
9	10806.5
10	10590.0
11	9910.0
12	9546.5
13	9499.0
14	9401.0
15	9654.0
16	9849.0
17	9998.5
18	10321.0
19	11065.5
20	12589.0
21	13483.0
22	15414.5
23	16683.0
24	18973.0
25	24675.5
26	28744.5
27	34649.5
28	44938.5
29	50642.0
30	53441.5
31	61192.5
32	72366.5
33	80162.5
34	87138.0
35	100692.0
36	108844.0
37	112374.0
38	124468.0
39	141117.5
40	161738.0
41	181082.0
42	193384.0
43	198877.0
44	205139.0
45	212267.5
46	213111.0
47	219515.5
48	218116.0
49	196344.0
50	182399.0
51	164101.5
52	134016.0
53	113794.0
54	99264.0
55	88919.5
56	78206.5
57	71742.0
58	64071.0
59	48016.5
60	35504.5
61	27069.0
62	20392.0
63	15351.5
64	12681.0
65	10557.5
66	7317.0
67	5272.0
68	3392.5
69	2446.5
70	1882.5
71	1313.0
72	1069.5
73	637.5
74	325.5
75	187.5
76	142.5
77	113.5
78	79.0
79	43.0
80	34.0
81	26.0
82	20.5
83	12.0
84	6.5
85	5.0
86	7.0
87	4.5
88	2.5
89	3.0
90	2.0
91	1.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3906623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.53823442082368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92163331976889	13.666078716155722
2	9.180303190049157	3.220126188026351
3	2.9449463554901802	1.5494747861801141
4	1.5755899613395703	1.105322643722796
5	0.9726825726350974	0.8529567487962098
6	0.6748350152533732	0.7101248815736271
7	0.5048241989436405	0.6197607599664667
8	0.3863785091129518	0.5421117494393046
9	0.2945999170805523	0.46500861655025505
>10	2.8941877847190307	11.799094332219031
>50	1.0849486259290044	13.866135973680786
>100	1.55326124760264	48.88895025238404
>500	0.0087093602810717	1.031118583153271
>1k	0.002952325519007356	0.9808933634255079
>5k	0.0	0.0
>10k+	1.476162759503678E-4	0.7028424047264488
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27148	0.6949224432457393	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0239022296238976E-4	0.0	0.0	0.10180147918035602	0.0
2	1.0239022296238976E-4	0.0	0.0	0.3191759225295095	0.0
3	1.0239022296238976E-4	0.0	0.0	0.4613703446685283	0.0
4	1.0239022296238976E-4	0.0	0.0	0.6799478731374898	0.0
5	1.0239022296238976E-4	0.0	0.0	1.2336741989180937	0.0
6	1.0239022296238976E-4	0.0	0.0	1.6973227260475352	0.0
7	1.0239022296238976E-4	0.0	0.0	1.9987083473373295	0.0
8	1.0239022296238976E-4	0.0	0.0	2.571530449700419	0.0
9	1.0239022296238976E-4	0.0	2.559755574059744E-5	2.7275987470508416	0.0
10	1.0239022296238976E-4	0.0	2.559755574059744E-5	3.013395456894612	0.0
11	1.0239022296238976E-4	0.0	2.559755574059744E-5	3.4706189975331636	0.0
12	1.0239022296238976E-4	0.0	2.559755574059744E-5	3.8207167673973146	0.0
13	1.0239022296238976E-4	0.0	2.559755574059744E-5	3.950675557892328	0.0
14	1.2798777870298722E-4	0.0	2.559755574059744E-5	4.001896266929263	0.0
15	1.2798777870298722E-4	0.0	2.559755574059744E-5	4.087033737322491	0.0
16	1.2798777870298722E-4	0.0	2.559755574059744E-5	4.268648395302029	0.0
17	1.2798777870298722E-4	0.0	2.559755574059744E-5	4.475886206577906	0.0
18	1.2798777870298722E-4	0.0	2.559755574059744E-5	4.711511707170105	0.0
19	1.2798777870298722E-4	0.0	2.559755574059744E-5	4.865250626948134	0.0
20	1.2798777870298722E-4	0.0	2.559755574059744E-5	5.030354861474987	0.0
21	1.2798777870298722E-4	0.0	2.559755574059744E-5	5.259197009795929	0.0
22	1.5358533444358464E-4	0.0	2.559755574059744E-5	5.509208336714344	0.0
23	1.5358533444358464E-4	0.0	2.559755574059744E-5	5.766668552353273	0.0
24	1.5358533444358464E-4	0.0	2.559755574059744E-5	5.956909586617393	0.0
25	1.5358533444358464E-4	0.0	2.559755574059744E-5	6.138063488593601	0.0
26	1.5358533444358464E-4	0.0	2.559755574059744E-5	6.301068723549726	0.0
27	1.5358533444358464E-4	0.0	2.559755574059744E-5	6.463408422056594	0.0
28	1.5358533444358464E-4	0.0	2.559755574059744E-5	6.639263629994499	0.0
29	1.5358533444358464E-4	0.0	2.559755574059744E-5	6.8271752866862245	0.0
30	1.5358533444358464E-4	0.0	2.559755574059744E-5	7.0336451712898835	0.0
31	1.5358533444358464E-4	0.0	2.559755574059744E-5	7.226113192903436	0.0
32	1.5358533444358464E-4	0.0	2.559755574059744E-5	7.403069095738186	0.0
33	1.5358533444358464E-4	0.0	2.559755574059744E-5	7.581688839696075	0.0
34	1.5358533444358464E-4	0.0	2.559755574059744E-5	7.769574898832061	0.0
35	1.5358533444358464E-4	0.0	2.559755574059744E-5	7.976787112552197	0.0
36	1.791828901841821E-4	0.0	2.559755574059744E-5	8.167616890598351	0.0
37	1.791828901841821E-4	0.0	2.559755574059744E-5	8.37022154428518	0.0
38	1.791828901841821E-4	0.0	2.559755574059744E-5	8.575539538880511	0.0
39	1.791828901841821E-4	0.0	2.559755574059744E-5	8.803332187416087	0.0
40	1.791828901841821E-4	0.0	2.559755574059744E-5	9.023445569229485	0.0
41	1.791828901841821E-4	0.0	2.559755574059744E-5	9.246809840621939	0.0
42	1.791828901841821E-4	0.0	2.559755574059744E-5	9.47135159957846	0.0
43	1.791828901841821E-4	0.0	2.559755574059744E-5	9.689801140268719	0.0
44	1.791828901841821E-4	0.0	2.559755574059744E-5	9.904027084261777	0.0
45	1.791828901841821E-4	0.0	2.559755574059744E-5	10.12086397894038	0.0
46	1.791828901841821E-4	0.0	2.559755574059744E-5	10.345815298788748	0.0
47	1.791828901841821E-4	0.0	2.559755574059744E-5	10.57378713021451	0.0
48	1.791828901841821E-4	0.0	2.559755574059744E-5	10.795845926264192	0.0
49	1.791828901841821E-4	0.0	2.559755574059744E-5	11.039585852026162	0.0
50	2.0478044592477952E-4	0.0	2.559755574059744E-5	11.270654987696535	0.0
51	2.0478044592477952E-4	0.0	2.559755574059744E-5	11.51854171748848	0.0
52	2.0478044592477952E-4	0.0	2.559755574059744E-5	11.768169081070786	0.0
53	2.0478044592477952E-4	0.0	2.559755574059744E-5	12.011525043496647	0.0
54	2.0478044592477952E-4	0.0	2.559755574059744E-5	12.255981700819353	0.0
55	2.0478044592477952E-4	0.0	2.559755574059744E-5	12.499568041246878	0.0
56	2.0478044592477952E-4	0.0	2.559755574059744E-5	12.754263720865822	0.0
57	2.0478044592477952E-4	0.0	2.559755574059744E-5	13.00831946159125	0.0
58	2.0478044592477952E-4	0.0	2.559755574059744E-5	13.253313667584509	0.0
59	2.3037800166537698E-4	0.0	2.559755574059744E-5	13.510057151662702	0.0
60	2.3037800166537698E-4	0.0	2.559755574059744E-5	13.76792692819348	0.0
61	2.3037800166537698E-4	0.0	2.559755574059744E-5	14.052290174915777	0.0
62	2.3037800166537698E-4	0.0	2.559755574059744E-5	14.34886345572634	0.0
63	2.3037800166537698E-4	0.0	2.559755574059744E-5	14.64364490763506	0.0
64	2.5597555740597443E-4	0.0	2.559755574059744E-5	14.923963740550342	0.0
65	2.5597555740597443E-4	0.0	2.559755574059744E-5	15.191279015149401	0.0
66	2.5597555740597443E-4	0.0	2.559755574059744E-5	15.455036229500518	0.0
67	2.5597555740597443E-4	0.0	2.559755574059744E-5	15.726882271465662	0.0
68	2.5597555740597443E-4	0.0	2.559755574059744E-5	16.002772727237822	0.0
69	2.5597555740597443E-4	0.0	2.559755574059744E-5	16.322179027768996	0.0
70	2.5597555740597443E-4	0.0	2.559755574059744E-5	16.600450056225032	0.0
71	2.5597555740597443E-4	0.0	2.559755574059744E-5	16.884122168942334	0.0
72	2.5597555740597443E-4	0.0	2.559755574059744E-5	17.17104517123869	0.0
73	2.5597555740597443E-4	0.0	2.559755574059744E-5	17.452797467275445	2.559755574059744E-5
74	2.5597555740597443E-4	0.0	2.559755574059744E-5	17.7687225002259	2.559755574059744E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15070	0.0	72.78036	1
TACGGGA	1400	0.0	68.85714	4
GCGCGAC	105	0.0	68.571434	9
TAGGGTA	3385	0.0	66.292465	5
TGTAGCG	315	0.0	63.492065	1
CTACGGG	780	0.0	63.076923	3
TTGCGGG	2875	0.0	62.886955	3
TAGGGAC	4675	0.0	62.545456	5
TAGCGGG	2435	0.0	61.930183	3
CGGGATC	1505	0.0	61.92691	6
ACGTAGG	575	0.0	61.913044	2
GTAGGGT	3265	0.0	61.868305	4
ATAGGGC	1400	0.0	61.714287	4
TTAGCGG	1155	0.0	61.64502	2
ATAGGGA	6145	0.0	61.643612	4
GGGACCG	1650	0.0	61.090908	7
CGTTAGG	730	0.0	60.82192	2
TTAGGGA	6645	0.0	60.556812	4
ACGGGAT	1895	0.0	60.369392	5
TTACGGG	1650	0.0	60.363636	3
>>END_MODULE