FastQCFastQC Report
Thu 26 May 2016
SRR1547772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547772_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2436699
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155660.638815052659356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37040.15200892683092987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG4050.073.0864262
GCGTAAG1100.072.727271
CGTTTTT77400.070.801031
TACGGGA12450.069.07634
TGCGTAG1450.068.9655151
CGTGCGG3150.068.571432
GGGCGAT18900.068.148157
ATTCGCG1650.067.878791
GGCGATT6700.067.4626858
AATGCGG3450.067.2463762
GCGCGAC1250.067.29
TCGTACC303.7968584E-666.6666780
ACGGGAT15250.066.360665
ACGGATT850.065.8823555
TAACGGG9700.065.154643
TATTGCG2400.065.000011
ACTACGG800.065.02
CACGGGA10500.064.04
CGAAACG2900.063.44827380
TAGCGCG1450.063.4482731
Produced by FastQC (version 0.11.5)