##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547772_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2436699
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.743214898516396	31.0	31.0	33.0	30.0	34.0
2	32.18641941413363	33.0	31.0	34.0	30.0	34.0
3	32.33436423620644	34.0	31.0	34.0	30.0	34.0
4	35.922463545969364	37.0	35.0	37.0	35.0	37.0
5	35.87080144080168	37.0	35.0	37.0	35.0	37.0
6	35.91460003882302	37.0	35.0	37.0	35.0	37.0
7	36.208578080427664	37.0	35.0	37.0	35.0	37.0
8	36.17008214802075	37.0	35.0	37.0	35.0	37.0
9	38.036327014538934	39.0	39.0	39.0	35.0	39.0
10-11	37.475797585175684	39.0	37.0	39.0	34.5	39.0
12-13	36.469675983779695	39.0	35.0	39.0	33.0	39.0
14-15	37.44308940086568	40.0	35.0	41.0	33.0	41.0
16-17	37.67039794410389	40.0	35.0	41.0	33.0	41.0
18-19	37.57156854416569	39.0	35.0	41.0	33.0	41.0
20-21	37.35503933805529	39.0	35.0	41.0	33.0	41.0
22-23	37.166982257554174	39.0	35.0	41.0	33.0	41.0
24-25	37.072571745628	38.5	35.0	41.0	33.0	41.0
26-27	37.05114234462279	39.0	35.0	41.0	33.0	41.0
28-29	37.066356985413464	39.0	35.0	41.0	33.0	41.0
30-31	36.902170518393945	38.0	35.0	41.0	32.5	41.0
32-33	36.59362953733719	38.0	35.0	41.0	31.5	41.0
34-35	36.3228031037071	38.0	35.0	41.0	31.0	41.0
36-37	36.09453260332934	38.0	35.0	41.0	30.0	41.0
38-39	36.03442259384519	38.0	35.0	41.0	30.0	41.0
40-41	35.87370598502318	38.0	35.0	41.0	30.0	41.0
42-43	35.83278135707364	38.0	35.0	41.0	30.0	41.0
44-45	35.72223528634436	38.0	35.0	40.0	29.5	41.0
46-47	35.6281479575442	37.5	35.0	40.0	29.0	41.0
48-49	35.53743158264521	37.0	35.0	40.0	29.0	41.0
50-51	35.35968188931008	37.0	35.0	40.0	29.0	41.0
52-53	35.17446102288383	36.5	34.0	40.0	28.0	41.0
54-55	34.982659942816085	36.0	34.0	40.0	28.0	41.0
56-57	34.66190982144286	36.0	34.0	40.0	26.5	41.0
58-59	34.36209868350584	35.5	34.0	39.0	26.0	41.0
60-61	34.04992245656932	35.0	34.0	39.0	26.0	41.0
62-63	33.63521633160271	35.0	33.0	39.0	24.5	40.0
64-65	33.342681636098675	35.0	33.0	38.0	24.5	40.0
66-67	33.07548613924001	35.0	33.0	37.0	23.0	40.0
68-69	32.69687823567868	35.0	33.0	37.0	22.5	39.5
70-71	32.44754789163537	35.0	32.5	36.0	22.0	39.0
72-73	32.218023851119895	35.0	32.0	36.0	20.5	39.0
74-75	31.937833519856163	35.0	32.0	35.5	20.0	38.0
76-77	29.886046450546417	33.0	29.0	34.5	17.5	36.0
78-79	30.9649474555536	34.0	30.5	35.0	18.5	37.0
80-81	31.283512243407987	35.0	31.5	35.0	19.0	36.0
82-83	31.160491509209795	35.0	31.5	35.0	18.5	36.0
84-85	30.977238879319934	34.0	31.0	35.0	18.0	36.0
86	30.562743695466693	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	12.0
11	18.0
12	49.0
13	120.0
14	448.0
15	1262.0
16	3016.0
17	6566.0
18	11842.0
19	17186.0
20	20705.0
21	21453.0
22	19758.0
23	18972.0
24	20353.0
25	22992.0
26	27488.0
27	33279.0
28	40432.0
29	49100.0
30	59651.0
31	72588.0
32	89437.0
33	119088.0
34	305483.0
35	274255.0
36	192245.0
37	329090.0
38	453512.0
39	225248.0
40	1044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.152021238569066	24.921994879137717	30.693122129569556	14.232861752723663
2	28.932995006769403	26.240623072443498	31.55346639039126	13.272915530395835
3	29.193634503071575	23.734650853470207	33.78045462324234	13.291260020215875
4	25.942350696577627	27.51222042607643	31.645517152508372	14.899911724837578
5	24.517143890156316	31.553876781662403	30.081803292076696	13.847176036104583
6	22.849149607727504	39.33834256918889	26.465025019503845	11.347482803579762
7	87.62756499674354	3.825749507838268	6.709897283168746	1.836788212249441
8	88.54983730038055	3.1375643852605517	6.353677659817647	1.9589206545412463
9	83.96671070165006	5.928266068152037	7.66262061912448	2.4424026110734234
10-11	43.03042764001626	27.15632911574224	19.219772323130595	10.593470921110896
12-13	30.4951698999343	27.901004596792628	26.619229539635388	14.984595963637693
14-15	15.196665653000228	32.67668677994286	36.40297796321991	15.723669603836996
16-17	20.091402343908708	25.802797144825853	33.64315822348185	20.462642287783595
18-19	23.731531879809527	27.766252622913214	30.513616987572124	17.988598509705138
20-21	25.940237181531245	27.812647356115793	30.04429352989434	16.202821932458626
22-23	21.180806492718222	25.14426279158813	31.231391320799162	22.443539394894486
24-25	19.090622190102266	26.66529595982105	35.78720638043517	18.456875469641513
26-27	16.639806557970434	34.72574987719041	30.66246590161526	17.971977663223896
28-29	15.452955001828292	29.568342253187613	36.55047258606828	18.428230158915813
30-31	22.439107169166157	29.52826754556061	30.83548686152865	17.197138423744583
32-33	25.709740924094444	29.365075456591068	27.83255133276617	17.09263228654832
34-35	19.61900095169736	29.958029284700327	30.180297197150736	20.24267256645158
36-37	22.770580198867403	29.57774021329676	30.323687907287688	17.327991680548152
38-39	19.51839353157694	31.058821791284032	28.98648950896274	20.43629516817629
40-41	20.035240298452948	27.605420283752736	29.15245173901249	23.206887678781825
42-43	21.302159191594857	26.111924369813423	29.30661522001692	23.2793012185748
44-45	18.426711711212587	32.43088292809247	28.45763879740583	20.684766563289106
46-47	20.69919181646974	30.804748555320128	28.274234938332555	20.221824689877575
48-49	21.34925158995838	26.54203494153361	31.673854669780717	20.434858798727294
50-51	18.591463287012473	31.266992763570716	29.580017884851596	20.561526064565218
52-53	21.687926986468167	26.537438559296817	30.940547026940955	20.834087427294058
54-55	21.90124836920769	27.255315490341648	31.354960132539965	19.488476007910702
56-57	22.000768252459576	24.901413756889955	30.909172614262165	22.1886453763883
58-59	23.6128056850682	27.653559179857666	28.45794659085919	20.27568854421494
60-61	24.334868607078675	29.32963817032797	27.30144346921799	19.034049753375363
62-63	22.383355514981538	26.3038438477629	30.927989054044012	20.384811583211548
64-65	18.8281154135164	30.872627271566984	31.076776409396484	19.222480905520133
66-67	17.988557470578023	29.57166642248386	26.406975174200838	26.03280093273728
68-69	17.40374990920093	32.55465693546885	25.93071610404075	24.11087705128947
70-71	19.04309888090404	29.92111458986112	29.097664504315055	21.938122024919778
72-73	22.484537482881553	30.305548613103216	27.283263135906406	19.92665076810882
74-75	20.946596194277586	32.07874669788923	25.019729560360144	21.954927547473034
76-77	26.138435645929185	28.628833516162643	26.250328826006005	18.982402011902167
78-79	18.358443123258144	30.12388891693229	30.483904659541455	21.033763300268106
80-81	16.32550840296647	29.36179232642193	31.915965000190834	22.39673427042076
82-83	18.417642064120354	27.26639605466248	32.273990345134955	22.041971536082215
84-85	16.553316597577297	27.32779058882529	30.26243701006977	25.856455803527638
86	17.38922205820251	27.654626197162635	31.149272027443686	23.80687971719117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2636.0
1	2750.0
2	6518.5
3	6584.5
4	3313.5
5	4491.5
6	5697.0
7	6042.0
8	6402.5
9	6420.5
10	5957.5
11	5713.5
12	5898.5
13	6097.0
14	5987.0
15	5905.5
16	5939.0
17	6101.0
18	6539.0
19	7488.0
20	8102.0
21	8158.0
22	8900.0
23	10663.0
24	12540.5
25	15106.0
26	19594.5
27	21517.0
28	23783.5
29	26907.0
30	33194.5
31	42629.5
32	47463.5
33	49393.0
34	53563.0
35	63421.5
36	69353.0
37	68616.0
38	72650.0
39	81545.5
40	93353.0
41	109300.5
42	126629.0
43	135693.0
44	138874.0
45	140973.0
46	137974.0
47	140187.0
48	140589.5
49	118230.5
50	99599.0
51	89121.0
52	74398.5
53	65240.5
54	58677.5
55	56505.5
56	51755.5
57	47528.0
58	43833.5
59	33538.5
60	23378.0
61	17450.0
62	13033.0
63	8958.5
64	6933.0
65	6105.5
66	4698.5
67	3596.0
68	2564.0
69	1874.5
70	1451.0
71	1208.0
72	950.0
73	576.0
74	398.0
75	248.0
76	116.0
77	70.0
78	68.5
79	69.0
80	48.0
81	23.5
82	19.5
83	21.5
84	23.0
85	15.5
86	9.0
87	7.0
88	3.0
89	1.0
90	1.0
91	0.5
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2436699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.140717676523083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7915516717336	12.08101356833422
2	9.370493985919149	2.8375200786071857
3	2.833271664302649	1.286932991102973
4	1.2575756208426774	0.7616238972822886
5	0.7294110421792602	0.5521903329887324
6	0.4852918254347185	0.4408599911778958
7	0.3646594727661657	0.3864844287655591
8	0.291198929523535	0.3527168623697269
9	0.2375354321128551	0.3236811224212488
>10	2.0466359469738364	6.701818533673817
>50	0.6816436244045981	7.609164497194021
>100	1.8869525577748818	63.45673104245449
>500	0.020498470717280608	1.8589415873824473
>1k	0.0030064423718678224	0.706175998145262
>5k	0.0	0.0
>10k+	2.733129428970747E-4	0.6441450681001553
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15566	0.638815052659356	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3704	0.15200892683092987	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.103912711418193E-5	0.0	0.0	0.049164874282789955	0.0
2	4.103912711418193E-5	0.0	0.0	0.1760578553198405	0.0
3	4.103912711418193E-5	0.0	0.0	0.26831381307252145	0.0
4	4.103912711418193E-5	0.0	0.0	0.43874930797771905	0.0
5	4.103912711418193E-5	0.0	0.0	0.7586493038327672	0.0
6	4.103912711418193E-5	0.0	0.0	1.03541717709081	0.0
7	4.103912711418193E-5	0.0	0.0	1.1981783552256557	0.0
8	4.103912711418193E-5	0.0	0.0	1.519473681402586	0.0
9	4.103912711418193E-5	0.0	0.0	1.6227691643489819	0.0
10	4.103912711418193E-5	0.0	0.0	1.7949283025929752	0.0
11	4.103912711418193E-5	0.0	0.0	2.1370304662167956	0.0
12	4.103912711418193E-5	0.0	0.0	2.3754677947501928	0.0
13	8.207825422836386E-5	0.0	0.0	2.458572027156411	0.0
14	8.207825422836386E-5	0.0	0.0	2.4980927065673684	0.0
15	8.207825422836386E-5	0.0	0.0	2.5599797102555546	0.0
16	8.207825422836386E-5	0.0	0.0	2.6789931788866825	0.0
17	8.207825422836386E-5	0.0	0.0	2.83137145786164	0.0
18	8.207825422836386E-5	0.0	0.0	3.008947760884705	0.0
19	8.207825422836386E-5	0.0	0.0	3.1284126599140887	0.0
20	8.207825422836386E-5	0.0	0.0	3.2486162632315274	0.0
21	8.207825422836386E-5	0.0	0.0	3.397588294656008	0.0
22	8.207825422836386E-5	0.0	0.0	3.549268908470024	0.0
23	8.207825422836386E-5	0.0	0.0	3.698733409419875	0.0
24	8.207825422836386E-5	0.0	0.0	3.8191011692457706	0.0
25	8.207825422836386E-5	0.0	0.0	3.922109378302367	0.0
26	8.207825422836386E-5	0.0	0.0	4.033489569290257	0.0
27	8.207825422836386E-5	0.0	0.0	4.164158150021812	0.0
28	8.207825422836386E-5	0.0	0.0	4.2830485012715975	0.0
29	8.207825422836386E-5	0.0	0.0	4.440638749390056	0.0
30	8.207825422836386E-5	0.0	0.0	4.596300158534148	0.0
31	8.207825422836386E-5	0.0	0.0	4.739813986052442	0.0
32	8.207825422836386E-5	0.0	0.0	4.875078949020786	0.0
33	8.207825422836386E-5	0.0	0.0	5.011616124929669	0.0
34	8.207825422836386E-5	0.0	0.0	5.168878060031214	0.0
35	8.207825422836386E-5	0.0	0.0	5.339108359300841	0.0
36	8.207825422836386E-5	0.0	0.0	5.479667369666914	0.0
37	8.207825422836386E-5	0.0	0.0	5.6446856997930395	0.0
38	8.207825422836386E-5	0.0	0.0	5.818404324867371	0.0
39	8.207825422836386E-5	0.0	0.0	6.062505052942526	0.0
40	8.207825422836386E-5	0.0	0.0	6.2462372250327185	0.0
41	1.231173813425458E-4	0.0	0.0	6.422459236861016	0.0
42	1.231173813425458E-4	0.0	0.0	6.6016771049686485	0.0
43	1.231173813425458E-4	0.0	0.0	6.791647224380196	0.0
44	1.231173813425458E-4	0.0	0.0	6.9676230014458085	0.0
45	1.231173813425458E-4	0.0	0.0	7.144501639307933	0.0
46	1.231173813425458E-4	0.0	0.0	7.337221380236131	0.0
47	1.231173813425458E-4	0.0	0.0	7.538477259604079	0.0
48	1.231173813425458E-4	0.0	0.0	7.731197000532277	0.0
49	1.231173813425458E-4	0.0	0.0	7.945995791847905	0.0
50	1.231173813425458E-4	0.0	0.0	8.154638714096407	0.0
51	1.231173813425458E-4	0.0	0.0	8.372269205182914	0.0
52	1.231173813425458E-4	0.0	0.0	8.626875949799299	0.0
53	1.231173813425458E-4	0.0	0.0	8.84023837166593	0.0
54	1.231173813425458E-4	0.0	0.0	9.063409144912852	0.0
55	1.231173813425458E-4	0.0	0.0	9.29068383087119	0.0
56	1.231173813425458E-4	0.0	0.0	9.512130960779317	0.0
57	1.231173813425458E-4	0.0	0.0	9.736943299110806	0.0
58	1.231173813425458E-4	0.0	0.0	9.948623116765756	0.0
59	1.231173813425458E-4	0.0	0.0	10.181766397901423	0.0
60	1.231173813425458E-4	0.0	0.0	10.426893104154432	0.0
61	1.6415650845672772E-4	0.0	0.0	10.681992318296187	0.0
62	1.6415650845672772E-4	0.0	0.0	10.982480807026226	0.0
63	2.0519563557090965E-4	0.0	0.0	11.259084523775813	0.0
64	2.0519563557090965E-4	0.0	0.0	11.544675809363405	0.0
65	2.0519563557090965E-4	0.0	0.0	11.79731267587831	0.0
66	2.0519563557090965E-4	0.0	0.0	12.043506399436287	0.0
67	2.0519563557090965E-4	0.0	0.0	12.30673136074665	0.0
68	2.0519563557090965E-4	0.0	0.0	12.611405840442336	0.0
69	2.0519563557090965E-4	0.0	0.0	13.067760933952039	0.0
70	2.0519563557090965E-4	0.0	0.0	13.347688819997874	0.0
71	2.0519563557090965E-4	0.0	0.0	13.627986058187737	0.0
72	2.0519563557090965E-4	0.0	0.0	13.895684284353546	0.0
73	2.0519563557090965E-4	0.0	0.0	14.178772183187172	0.0
74	2.0519563557090965E-4	0.0	0.0	14.492721505610664	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	405	0.0	73.086426	2
GCGTAAG	110	0.0	72.72727	1
CGTTTTT	7740	0.0	70.80103	1
TACGGGA	1245	0.0	69.0763	4
TGCGTAG	145	0.0	68.965515	1
CGTGCGG	315	0.0	68.57143	2
GGGCGAT	1890	0.0	68.14815	7
ATTCGCG	165	0.0	67.87879	1
GGCGATT	670	0.0	67.462685	8
AATGCGG	345	0.0	67.246376	2
GCGCGAC	125	0.0	67.2	9
TCGTACC	30	3.7968584E-6	66.66667	80
ACGGGAT	1525	0.0	66.36066	5
ACGGATT	85	0.0	65.882355	5
TAACGGG	970	0.0	65.15464	3
TATTGCG	240	0.0	65.00001	1
ACTACGG	80	0.0	65.0	2
CACGGGA	1050	0.0	64.0	4
CGAAACG	290	0.0	63.448273	80
TAGCGCG	145	0.0	63.448273	1
>>END_MODULE