FastQCFastQC Report
Thu 26 May 2016
SRR1547771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547771_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2089992
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114500.5478489869817683No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38000.1818188777756087No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT37770.1807183950943353No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT26650.1275124498084203No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT24830.11880428250443063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG900.071.111112
GGCGATA2500.068.88
CGTTTTT57450.068.2332461
CGGGATC5300.067.169816
TCGATAG850.065.8823551
TCGTAGG3750.065.0666662
CGAGGGA12050.065.062244
TTGCGGG11400.064.9122853
CCGATCC30750.064.080
GCGGGAT12950.063.629345
TAGGGCG3400.063.5294155
TTTGCGG6400.063.1252
GGGCGAT12200.062.950827
GTTAGCG700.062.8571431
ATAGGGC7700.062.857144
AGGGATT30750.062.5691076
TAAGGGA24750.062.5454564
TAGGGTA16700.062.2754485
TTAGGGA24750.062.0606084
TTGAGGG37150.061.9111673
Produced by FastQC (version 0.11.5)