##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547771_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2089992
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.813517468009447	31.0	31.0	33.0	30.0	34.0
2	32.254914851348715	33.0	31.0	34.0	31.0	34.0
3	32.400953687861005	34.0	31.0	34.0	31.0	34.0
4	36.00196507929217	37.0	35.0	37.0	35.0	37.0
5	35.962291721690804	37.0	35.0	37.0	35.0	37.0
6	36.0247924393969	37.0	35.0	37.0	35.0	37.0
7	36.25170670509743	37.0	35.0	37.0	35.0	37.0
8	36.19580170641802	37.0	36.0	37.0	35.0	37.0
9	38.06803662406363	39.0	39.0	39.0	37.0	39.0
10-11	37.576075410814965	39.0	37.5	39.0	35.0	39.0
12-13	36.815805036574304	39.0	35.0	39.0	33.0	39.0
14-15	37.83861517173271	40.0	35.0	41.0	33.0	41.0
16-17	37.99300762873733	40.0	35.0	41.0	33.5	41.0
18-19	37.889644314428	40.0	36.0	41.0	33.5	41.0
20-21	37.72265539772401	40.0	35.0	41.0	33.5	41.0
22-23	37.54627529674755	39.0	35.0	41.0	33.0	41.0
24-25	37.44265719677396	39.0	35.0	41.0	33.0	41.0
26-27	37.43695884960326	39.0	35.0	41.0	33.0	41.0
28-29	37.43017533081466	39.0	35.0	41.0	33.0	41.0
30-31	37.258600032918785	39.0	35.0	41.0	33.0	41.0
32-33	36.9929815520825	39.0	35.0	41.0	32.5	41.0
34-35	36.71980873611	39.0	35.0	41.0	31.0	41.0
36-37	36.53524128322022	39.0	35.0	41.0	31.0	41.0
38-39	36.42279587673063	39.0	35.0	41.0	31.0	41.0
40-41	36.27546397306784	38.5	35.0	41.0	30.0	41.0
42-43	36.23828033791517	38.0	35.0	41.0	30.0	41.0
44-45	36.11756982801848	38.0	35.0	41.0	30.0	41.0
46-47	36.03245227732929	38.0	35.0	41.0	30.0	41.0
48-49	35.914146322091185	38.0	35.0	40.0	30.0	41.0
50-51	35.72246448790234	37.5	35.0	40.0	29.5	41.0
52-53	35.55634854104704	37.0	35.0	40.0	29.0	41.0
54-55	35.33124409088647	37.0	34.5	40.0	29.0	41.0
56-57	35.01121009075633	36.0	34.0	40.0	28.0	41.0
58-59	34.72094055862415	36.0	34.0	39.5	27.0	41.0
60-61	34.41669944191174	35.5	34.0	39.0	26.5	41.0
62-63	34.023379754563656	35.0	33.5	39.0	26.0	40.5
64-65	33.72149821626111	35.0	33.0	38.0	26.0	40.0
66-67	33.43124471289842	35.0	33.0	37.5	25.5	40.0
68-69	33.041781738877475	35.0	33.0	37.0	24.5	39.5
70-71	32.81069138063687	35.0	33.0	36.5	24.0	39.0
72-73	32.56424354734372	35.0	33.0	36.0	24.0	39.0
74-75	32.28628937335645	35.0	32.5	35.5	23.5	38.0
76-77	30.248207648641717	33.0	29.5	34.5	19.5	36.0
78-79	31.37934307882518	34.0	31.5	35.0	20.0	37.0
80-81	31.596973576932353	35.0	32.0	35.0	20.0	36.0
82-83	31.458230222890805	35.0	32.0	35.0	20.0	36.0
84-85	31.26386201478283	34.5	32.0	35.0	19.5	36.0
86	30.86439756707203	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	8.0
10	9.0
11	23.0
12	25.0
13	107.0
14	277.0
15	874.0
16	1992.0
17	4329.0
18	8644.0
19	12950.0
20	15461.0
21	16085.0
22	15032.0
23	14022.0
24	14533.0
25	16255.0
26	19417.0
27	23869.0
28	29657.0
29	37100.0
30	46457.0
31	57838.0
32	73571.0
33	99947.0
34	239106.0
35	218336.0
36	191696.0
37	323075.0
38	417254.0
39	191254.0
40	780.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.85300039425988	24.25511676599719	28.401448426596847	13.49043441314608
2	32.14773070901707	24.840525705361554	29.416715470681225	13.595028114940153
3	30.135426355698968	24.76363545889171	32.32289884363194	12.778039341777383
4	27.192113654023558	26.869720075483546	30.412604450160575	15.525561820332326
5	23.447362477942498	31.977634364150674	29.30470547255683	15.270297685349993
6	21.753241160731715	41.134080896003425	26.939337566842358	10.173340376422493
7	87.67425904022599	4.214513739765511	6.386435928941355	1.7247912910671428
8	88.02076754360783	3.6163296318837586	6.473374060761955	1.889528763746464
9	83.56582226152062	5.7557636584254865	7.8595994625816745	2.8188146174722197
10-11	40.10883773717794	26.777351300866226	19.785291044176244	13.328519917779587
12-13	29.920999697606497	26.13605698012241	27.44874143058921	16.494201891681882
14-15	17.41643030212556	31.06035812577273	34.488050671964295	17.035160900137416
16-17	22.05491695661993	24.519519691941404	32.76813978235323	20.65742356908543
18-19	24.73964015173264	27.489794219308017	29.431332751512922	18.33923287744642
20-21	27.049648993871745	27.61582340985037	28.19977301348522	17.13475458279266
22-23	22.56058874866507	25.212441004558865	30.369350696079216	21.857619550696842
24-25	21.11991337765886	27.174912631244517	32.93072413674311	18.77444985435351
26-27	19.283901565173455	32.04167767149348	29.064154312552393	19.61026645078067
28-29	17.751910055158106	28.8175744213375	34.54317050017416	18.887345023330234
30-31	23.420687734689892	27.603837718039117	30.04767482363569	18.927799723635307
32-33	25.672466688867708	28.27395990032498	27.957595052995423	18.095978357811894
34-35	20.409958507018207	27.641804370543042	30.36360904730736	21.584628075131388
36-37	23.71733958790273	28.137380430164328	29.63097466401785	18.514305317915092
38-39	20.66134702907954	30.168703995039216	27.76955605571696	21.400392920164286
40-41	21.2880001454551	26.17818632798594	29.579180207388355	22.9546333191706
42-43	21.510249799999233	26.588642444564382	28.59010943582559	23.310998319610793
44-45	20.77921829365854	29.504179920305916	28.29419921224579	21.422402573789757
46-47	22.225587466363507	29.15874797606881	27.74079996478455	20.87486459278313
48-49	21.655106813805986	27.657043663325027	29.81913327897906	20.868716243889928
50-51	19.44524189566276	30.24155116383221	29.060757170362372	21.25244977014266
52-53	22.75965649629281	26.325052918862845	29.235016210588366	21.68027437425598
54-55	21.942476334837647	26.77256659355634	30.6599738180816	20.624983253524416
56-57	23.520161799662393	25.30610165014986	29.418820741897576	21.754915808290175
58-59	23.5074823252912	27.08541468101313	28.61180808347592	20.795294910219752
60-61	24.53384989033451	28.8278854655903	27.025318757200985	19.612945886874208
62-63	24.40791639393835	25.696725154928824	29.788965699390236	20.10639275174259
64-65	21.59898219706104	29.473533869986106	29.08183380606242	19.845650126890437
66-67	20.077804125566033	29.307743761698607	26.763332108448264	23.8511200042871
68-69	19.94945435197838	30.793921699221816	26.78790158048452	22.468722368315287
70-71	20.239910009224914	29.639539290102547	28.841928581544813	21.27862211912773
72-73	23.259586639566084	29.37341386952677	27.2979035326451	20.06909595826204
74-75	21.284076685461	30.98337218515669	25.226795126488522	22.50575600289379
76-77	24.971172138457945	28.023839325700767	26.69000646892428	20.314982066917004
78-79	19.961296502570345	28.611377459818026	29.13803019341701	22.28929584419462
80-81	18.273562769618255	27.838623305735144	29.69688400721151	24.190929917435092
82-83	19.99457414191059	27.656780504422983	30.655332651991014	21.693312701675413
84-85	18.462534784822143	27.00431389211059	29.585400326891204	24.94775099617606
86	18.75834931425575	27.228573123724875	30.206479259250752	23.806598302768624
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1264.0
1	1628.5
2	4815.0
3	4904.5
4	2400.5
5	2883.0
6	3515.5
7	3894.0
8	3971.5
9	4098.0
10	3993.0
11	3826.5
12	3915.5
13	4087.0
14	4157.0
15	4140.0
16	4467.5
17	4776.0
18	5006.5
19	5500.0
20	5935.0
21	6143.0
22	6752.0
23	8411.5
24	10113.0
25	10998.5
26	12146.0
27	15905.0
28	21101.0
29	23451.0
30	26291.5
31	31031.0
32	34267.5
33	39205.0
34	43890.5
35	51757.0
36	58538.0
37	56799.0
38	56096.5
39	59097.5
40	68069.5
41	80355.0
42	90010.0
43	94387.0
44	103102.0
45	107245.0
46	106811.5
47	117315.5
48	121820.0
49	114583.5
50	109208.0
51	95130.5
52	75203.5
53	65797.5
54	63583.5
55	62827.0
56	56572.0
57	52416.0
58	48760.5
59	38155.0
60	27255.5
61	21813.0
62	18907.0
63	14907.5
64	12321.0
65	10039.0
66	6672.5
67	4743.0
68	3680.0
69	3193.0
70	2234.5
71	1545.0
72	1213.0
73	600.5
74	296.0
75	223.0
76	140.0
77	103.5
78	86.0
79	71.0
80	56.0
81	29.5
82	22.5
83	22.5
84	16.5
85	9.5
86	4.0
87	5.0
88	4.0
89	5.5
90	6.5
91	3.0
92	1.5
93	1.0
94	1.5
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2089992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.526039738665688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.46868347212916	12.338339375677226
2	8.927427602711733	2.7721519144752658
3	2.7037611712134137	1.2593611016436221
4	1.287377782509145	0.799515144396492
5	0.7713472377644385	0.598798393292034
6	0.5095497421944335	0.4746773727682578
7	0.385627134615009	0.4191083551438288
8	0.30221517553977834	0.37537638600467393
9	0.25878233646307897	0.36160783556314663
>10	2.5711855082929156	9.103663776411995
>50	0.9872308942481889	11.240150623812285
>100	1.806918125012586	56.67775877438513
>500	0.013987840293403348	1.4483210411660488
>1k	0.00559513611736134	1.5785782384371974
>5k	0.0	0.0
>10k+	3.1084089540896334E-4	0.5525916668227677
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11450	0.5478489869817683	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3800	0.1818188777756087	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3777	0.1807183950943353	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2665	0.1275124498084203	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2483	0.11880428250443063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.78470730988444E-5	0.0	0.0	0.06909117355473131	0.0
2	4.78470730988444E-5	0.0	0.0	0.2063644262753159	0.0
3	4.78470730988444E-5	0.0	0.0	0.2798575305551409	0.0
4	4.78470730988444E-5	0.0	0.0	0.37507320602184124	0.0
5	4.78470730988444E-5	0.0	0.0	0.5848826215602739	0.0
6	4.78470730988444E-5	0.0	0.0	0.7443090691256234	0.0
7	4.78470730988444E-5	0.0	0.0	0.8461754877530632	0.0
8	4.78470730988444E-5	0.0	0.0	1.0713916608293237	0.0
9	4.78470730988444E-5	0.0	0.0	1.146990036325498	0.0
10	4.78470730988444E-5	0.0	0.0	1.302205941458149	0.0
11	4.78470730988444E-5	0.0	0.0	1.5637380430164325	0.0
12	4.78470730988444E-5	0.0	0.0	1.7655091502742595	0.0
13	4.78470730988444E-5	0.0	0.0	1.8452223740569342	0.0
14	4.78470730988444E-5	0.0	0.0	1.884983291802074	0.0
15	4.78470730988444E-5	0.0	0.0	1.9422562383013906	0.0
16	4.78470730988444E-5	0.0	0.0	2.063548568606961	0.0
17	4.78470730988444E-5	0.0	0.0	2.209386447412239	0.0
18	4.78470730988444E-5	0.0	0.0	2.4094350600385073	0.0
19	4.78470730988444E-5	0.0	0.0	2.5212058227974077	0.0
20	4.78470730988444E-5	0.0	0.0	2.644699118465525	0.0
21	4.78470730988444E-5	0.0	0.0	2.7865178431305	0.0
22	4.78470730988444E-5	0.0	0.0	2.944604572649082	0.0
23	4.78470730988444E-5	0.0	0.0	3.098959230465954	0.0
24	4.78470730988444E-5	0.0	0.0	3.2264238332012756	0.0
25	4.78470730988444E-5	0.0	0.0	3.3330749591385995	0.0
26	4.78470730988444E-5	0.0	0.0	3.441687815072976	0.0
27	4.78470730988444E-5	0.0	0.0	3.5704921358550656	0.0
28	4.78470730988444E-5	0.0	0.0	3.7002055510260328	0.0
29	4.78470730988444E-5	0.0	0.0	3.848579324705549	0.0
30	4.78470730988444E-5	0.0	0.0	4.01704886908658	0.0
31	4.78470730988444E-5	0.0	0.0	4.166140348862579	0.0
32	4.78470730988444E-5	0.0	0.0	4.3057102610919085	0.0
33	4.78470730988444E-5	0.0	0.0	4.4458064911253246	0.0
34	4.78470730988444E-5	0.0	0.0	4.605041550398279	0.0
35	4.78470730988444E-5	0.0	0.0	4.776094836726648	0.0
36	4.78470730988444E-5	0.0	0.0	4.9312628947862	0.0
37	4.78470730988444E-5	0.0	0.0	5.092507531129305	0.0
38	4.78470730988444E-5	0.0	0.0	5.273943632320123	0.0
39	4.78470730988444E-5	0.0	0.0	5.555332269214428	0.0
40	4.78470730988444E-5	0.0	0.0	5.749687080141934	0.0
41	4.78470730988444E-5	0.0	0.0	5.951793116911452	0.0
42	4.78470730988444E-5	0.0	0.0	6.152511588561104	0.0
43	4.78470730988444E-5	0.0	0.0	6.328540970491753	0.0
44	4.78470730988444E-5	0.0	0.0	6.511890954606525	0.0
45	4.78470730988444E-5	0.0	0.0	6.698350998472722	0.0
46	4.78470730988444E-5	0.0	0.0	6.891461785499658	0.0
47	4.78470730988444E-5	0.0	0.0	7.09423768129256	0.0
48	4.78470730988444E-5	0.0	0.0	7.293520740749247	0.0
49	4.78470730988444E-5	0.0	0.0	7.494574141910591	0.0
50	4.78470730988444E-5	0.0	0.0	7.702278286232675	0.0
51	4.78470730988444E-5	0.0	0.0	7.917207338592684	0.0
52	4.78470730988444E-5	0.0	0.0	8.164289624075115	0.0
53	4.78470730988444E-5	0.0	0.0	8.394481892753657	0.0
54	4.78470730988444E-5	0.0	0.0	8.619889454122312	0.0
55	4.78470730988444E-5	0.0	0.0	8.836684542333176	0.0
56	4.78470730988444E-5	0.0	0.0	9.052570536155162	0.0
57	4.78470730988444E-5	0.0	0.0	9.280801074836651	0.0
58	9.56941461976888E-5	0.0	0.0	9.496447833293141	0.0
59	9.56941461976888E-5	0.0	0.0	9.741711929997818	0.0
60	9.56941461976888E-5	0.0	0.0	10.006354091307527	0.0
61	9.56941461976888E-5	0.0	0.0	10.268747440181588	0.0
62	9.56941461976888E-5	0.0	0.0	10.588270194335673	0.0
63	9.56941461976888E-5	0.0	0.0	10.871668408300128	0.0
64	9.56941461976888E-5	0.0	0.0	11.145162278133123	0.0
65	9.56941461976888E-5	0.0	0.0	11.40200536652772	0.0
66	9.56941461976888E-5	0.0	0.0	11.638848378367	0.0
67	9.56941461976888E-5	0.0	0.0	11.90459102235798	0.0
68	9.56941461976888E-5	0.0	0.0	12.193348108509506	0.0
69	9.56941461976888E-5	0.0	0.0	12.59071805059541	0.0
70	1.435412192965332E-4	0.0	0.0	12.865073167744184	0.0
71	1.435412192965332E-4	0.0	0.0	13.151629288533162	0.0
72	1.435412192965332E-4	0.0	0.0	13.434166255181838	0.0
73	1.435412192965332E-4	0.0	0.0	13.729813319859598	0.0
74	1.435412192965332E-4	0.0	0.0	14.054503557908356	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	90	0.0	71.11111	2
GGCGATA	250	0.0	68.8	8
CGTTTTT	5745	0.0	68.233246	1
CGGGATC	530	0.0	67.16981	6
TCGATAG	85	0.0	65.882355	1
TCGTAGG	375	0.0	65.066666	2
CGAGGGA	1205	0.0	65.06224	4
TTGCGGG	1140	0.0	64.912285	3
CCGATCC	3075	0.0	64.0	80
GCGGGAT	1295	0.0	63.62934	5
TAGGGCG	340	0.0	63.529415	5
TTTGCGG	640	0.0	63.125	2
GGGCGAT	1220	0.0	62.95082	7
GTTAGCG	70	0.0	62.857143	1
ATAGGGC	770	0.0	62.85714	4
AGGGATT	3075	0.0	62.569107	6
TAAGGGA	2475	0.0	62.545456	4
TAGGGTA	1670	0.0	62.275448	5
TTAGGGA	2475	0.0	62.060608	4
TTGAGGG	3715	0.0	61.911167	3
>>END_MODULE