FastQCFastQC Report
Thu 26 May 2016
SRR1547770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547770_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2249774
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185850.8260829754455336No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT41980.18659652036160077No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34950.15534893727103255No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT27220.12098993054413466No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT26660.11850079163507089No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC23250.10334371363523624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82950.070.548521
GGCGATC1350.068.148158
GATCGCG303.7967657E-666.6666641
GTACGAT559.094947E-1265.4545449
TAGGGCA22450.064.320725
CGTAAGG2650.063.396232
TTACGGG9300.063.2258033
CGAGGGC9050.063.204424
GGGCGAT14050.063.2028477
AGGGTAA20500.062.6341486
GTAGGGT19800.062.2222254
TAGGGAC20400.062.1568645
AGTAGGG41400.062.1256033
GTCGAAG2200.061.818181
CCGATCC35650.061.7110880
TAGGGTA19500.061.538465
AAGGGTC15450.060.3236245
GCACGGG7900.060.2531623
TGCGGGC8950.059.888274
CGAGGGA14700.059.8639454
Produced by FastQC (version 0.11.5)