##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547770_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2249774 Sequences flagged as poor quality 0 Sequence length 86 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72464256409755 31.0 31.0 33.0 30.0 34.0 2 32.173239178690835 33.0 31.0 34.0 30.0 34.0 3 32.32573138457463 34.0 31.0 34.0 30.0 34.0 4 35.930145427940765 37.0 35.0 37.0 35.0 37.0 5 35.86436148697602 37.0 35.0 37.0 35.0 37.0 6 35.924651098288095 37.0 35.0 37.0 35.0 37.0 7 36.20961172099953 37.0 35.0 37.0 35.0 37.0 8 36.16985528324178 37.0 35.0 37.0 35.0 37.0 9 38.045545019188594 39.0 39.0 39.0 35.0 39.0 10-11 37.446135478496956 39.0 37.0 39.0 34.5 39.0 12-13 36.72984775359659 39.0 35.0 39.0 33.0 39.0 14-15 37.71776476214944 40.0 35.0 41.0 33.0 41.0 16-17 37.879950386127675 40.0 35.0 41.0 33.0 41.0 18-19 37.7705071709425 40.0 36.0 41.0 33.0 41.0 20-21 37.582748311608185 39.5 35.0 41.0 33.0 41.0 22-23 37.38747047481213 39.0 35.0 41.0 33.0 41.0 24-25 37.30669569476756 39.0 35.0 41.0 33.0 41.0 26-27 37.265233974612556 39.0 35.0 41.0 33.0 41.0 28-29 37.2462838489555 39.0 35.0 41.0 33.0 41.0 30-31 37.0381973922714 39.0 35.0 41.0 32.0 41.0 32-33 36.731270563176565 39.0 35.0 41.0 31.0 41.0 34-35 36.440386234350655 39.0 35.0 41.0 30.5 41.0 36-37 36.24989998995454 39.0 35.0 41.0 30.0 41.0 38-39 36.155020904321944 38.0 35.0 41.0 30.0 41.0 40-41 36.021547719904305 38.0 35.0 41.0 30.0 41.0 42-43 35.965229174130386 38.0 35.0 41.0 30.0 41.0 44-45 35.809257507643 38.0 35.0 41.0 29.0 41.0 46-47 35.73699758286833 38.0 35.0 40.0 29.0 41.0 48-49 35.62013117762051 38.0 35.0 40.0 29.0 41.0 50-51 35.44627460358241 37.0 35.0 40.0 28.0 41.0 52-53 35.24838450439911 37.0 34.5 40.0 28.0 41.0 54-55 35.04246670998954 36.5 34.0 40.0 27.5 41.0 56-57 34.71468267479311 36.0 34.0 40.0 26.5 41.0 58-59 34.39652849575113 36.0 34.0 39.0 26.0 41.0 60-61 34.078432767024594 35.0 34.0 39.0 26.0 41.0 62-63 33.684526312420715 35.0 33.0 39.0 25.0 40.0 64-65 33.354902536877034 35.0 33.0 38.0 24.5 40.0 66-67 33.0724557222192 35.0 33.0 37.0 23.5 40.0 68-69 32.64897251901746 35.0 32.5 37.0 22.5 39.5 70-71 32.40011018884563 35.0 32.0 36.0 22.0 39.0 72-73 32.132830675436736 35.0 32.0 36.0 20.5 38.5 74-75 31.83358950721272 35.0 32.0 35.0 20.0 37.5 76-77 29.83090345963639 33.0 29.0 34.5 18.0 35.5 78-79 30.95980285130862 34.0 30.5 35.0 19.0 36.0 80-81 31.160273654153706 34.5 31.0 35.0 19.0 36.0 82-83 31.03185586641147 34.5 31.0 35.0 18.5 36.0 84-85 30.84527201398896 34.0 31.0 35.0 18.0 36.0 86 30.451928949307796 34.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 4.0 9 14.0 10 13.0 11 15.0 12 32.0 13 115.0 14 403.0 15 1120.0 16 2784.0 17 6104.0 18 11417.0 19 16325.0 20 19124.0 21 19108.0 22 17922.0 23 17261.0 24 18459.0 25 21404.0 26 25608.0 27 30390.0 28 36246.0 29 44637.0 30 54524.0 31 67126.0 32 82011.0 33 107003.0 34 244746.0 35 230857.0 36 204413.0 37 341483.0 38 443889.0 39 184576.0 40 639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.420332886769962 24.288306292098675 31.98970207674193 14.301658744389437 2 30.742110096391905 26.101199498260712 29.300854219134898 13.85583618621248 3 28.74844317695911 25.929537811353498 32.56242627037204 12.759592741315352 4 27.569569210062877 26.645209696618416 31.574371470201008 14.2108496231177 5 23.88964402646666 31.65464620001831 30.357804828396095 14.097904945118934 6 21.364723745585113 42.28753643699323 26.57680282552825 9.770936991893409 7 88.56560703430657 3.5114193692344213 6.510609510110793 1.4123640863482287 8 89.43818356866068 2.812238029242048 6.23884887993194 1.5107295221653374 9 84.93595356689161 4.960853845764063 7.543024321554076 2.560168265790253 10-11 38.464796908489475 24.78235591663874 20.761196457955332 15.991650716916455 12-13 30.619164413847795 26.085931297988154 25.883822108353993 17.411082179810062 14-15 18.03049995243967 31.83815352119813 32.85687806864156 17.274468457720644 16-17 22.893010586841168 26.0887538037154 31.103546400660687 19.91468920878275 18-19 24.62707365273134 28.80235970368579 28.846475245958036 17.72409139762483 20-21 25.963807920262212 28.71348410995949 28.233124749419275 17.08958322035902 22-23 21.49442566231097 25.90273511917197 31.19966716656873 21.40317205194833 24-25 20.571510738411945 28.78271328586782 32.328802804192776 18.31697317152745 26-27 18.583622177160905 32.75709026773356 29.28447479613508 19.374812758970457 28-29 17.706534078534112 29.79117013531137 33.7760148352679 18.726280950886622 30-31 23.02366815511247 28.439856625598836 30.365494489668738 18.170980729619952 32-33 24.8210709164565 29.276251747953353 27.38661750024669 18.516059835343462 34-35 20.30863989005118 29.174730439590824 29.861466085037875 20.655163585320125 36-37 22.45081061475508 30.45481457248595 29.021515049956133 18.072859762802842 38-39 20.16631448314364 31.181598685023477 27.769300383060695 20.882786448772187 40-41 20.847382892681665 27.898735606332014 29.334302023225444 21.919579477760877 42-43 21.634861990582166 27.461180545245877 28.311288156054786 22.592669308117173 44-45 20.582022905411833 30.820918012209226 27.9851442856038 20.611914796775142 46-47 21.289116151222302 30.306288542760296 28.305243104418487 20.099352201598915 48-49 21.520672743128866 29.390774362224825 29.31156640622569 19.776986488420615 50-51 19.42417327251537 30.849720905299822 29.01711460795618 20.70899121422863 52-53 22.039769327941382 27.508363062245362 29.522365357587027 20.92950225222622 54-55 21.261957867768054 27.609173188062446 30.30384385276032 20.825025091409184 56-57 22.516439429027095 26.308909250440266 29.672736017039934 21.501915303492705 58-59 22.376580936574072 28.783446692867816 28.42629970832626 20.413672662231853 60-61 23.88968847537575 30.248771654397288 27.029114924432406 18.832424945794553 62-63 23.366724835472365 27.794569587878605 29.016514547683457 19.822191028965577 64-65 21.690778718217917 30.257661436215372 28.728774534686597 19.322785310880114 66-67 19.492357899060085 31.35846089429427 26.46585834843855 22.68332285820709 68-69 19.23308741233564 32.78633764991506 26.12609088735135 21.85448405039795 70-71 19.856350015601567 30.611963690575145 28.3803617607813 21.151324533041986 72-73 22.782177232024196 30.505286308758123 27.15132719997653 19.56120925924115 74-75 21.473156859311203 32.40114340373744 25.124568067725917 21.001131669225444 76-77 23.661976714105506 29.332190700043647 26.86440949179784 20.141423094053003 78-79 19.635927875422155 29.57783759613188 28.424277282962645 22.361957245483325 80-81 18.096106542257136 29.818928479038338 28.969398704047606 23.11556627465692 82-83 19.72060304723941 29.019581522410697 30.00303586049088 21.256779569859017 84-85 17.86606121325964 28.012258120148957 29.34108048186173 24.780600184729668 86 19.28420365778963 28.183275297874367 28.870322085685054 23.662198958650958 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1688.0 1 2440.5 2 7537.5 3 7596.5 4 4032.0 5 6062.5 6 8390.0 7 9408.0 8 9467.5 9 9216.5 10 7997.5 11 6451.0 12 5937.5 13 5968.0 14 5874.0 15 5808.5 16 5777.0 17 6032.5 18 6405.5 19 6503.0 20 7098.5 21 7748.0 22 7963.5 23 8423.0 24 9982.5 25 12137.5 26 12864.0 27 14859.5 28 19616.5 29 22265.0 30 24805.5 31 30592.0 32 34880.5 33 39565.0 34 45450.5 35 50066.5 36 52439.0 37 55038.0 38 60766.0 39 68628.5 40 78530.0 41 89554.5 42 102756.0 43 110101.0 44 111399.0 45 115191.0 46 121041.5 47 126475.0 48 131115.5 49 124866.0 50 116053.0 51 103173.0 52 83754.0 53 74290.5 54 69597.5 55 65621.5 56 58186.5 57 52959.0 58 47864.5 59 36309.5 60 26235.0 61 20454.0 62 15222.0 63 10498.5 64 8840.0 65 7095.5 66 4762.5 67 3428.5 68 2473.0 69 1848.0 70 1174.5 71 916.0 72 663.5 73 314.5 74 164.5 75 176.5 76 170.5 77 64.0 78 27.0 79 25.0 80 18.0 81 11.5 82 15.5 83 11.0 84 4.0 85 5.0 86 5.0 87 3.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 86 2249774.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.455181734235342 #Duplication Level Percentage of deduplicated Percentage of total 1 79.383266064054 13.062660697411657 2 8.693728220884472 2.86113755645409 3 2.6832300290972775 1.3245911329065987 4 1.3270545738634505 0.8734769673668528 5 0.7993307370659289 0.6576566272090074 6 0.5527413289138177 0.5457275411579775 7 0.4195886749364585 0.48330855297944963 8 0.3604002332076823 0.47443610675945685 9 0.29353604956355406 0.4347170137006016 >10 2.903004672538844 10.501273596038361 >50 0.9517591930841254 11.436868733765078 >100 1.60840296802034 53.12853445333046 >500 0.019056907203530624 1.9476308379704728 >1k 0.004628106035143152 1.4354123495779254 >5k 0.0 0.0 >10k+ 2.722415314790089E-4 0.8325678333720037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18585 0.8260829754455336 No Hit AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 4198 0.18659652036160077 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 3495 0.15534893727103255 No Hit TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2722 0.12098993054413466 No Hit GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2666 0.11850079163507089 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC 2325 0.10334371363523624 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.027380527999701303 0.0 2 0.0 0.0 0.0 0.12592375945317175 0.0 3 0.0 0.0 0.0 0.20682077399774376 0.0 4 0.0 0.0 0.0 0.3187431270874319 0.0 5 0.0 0.0 0.0 0.5807694461754825 0.0 6 0.0 0.0 0.0 0.8019472178094333 0.0 7 0.0 0.0 0.0 0.9448060116260567 0.0 8 0.0 0.0 0.0 1.2129218312594954 0.0 9 0.0 0.0 0.0 1.3104871867129766 0.0 10 0.0 0.0 0.0 1.4857492352565191 0.0 11 0.0 0.0 0.0 1.8627204332524068 0.0 12 0.0 0.0 0.0 2.1059004148861176 0.0 13 0.0 0.0 0.0 2.1955983134305934 0.0 14 0.0 0.0 0.0 2.231290787430204 0.0 15 0.0 0.0 0.0 2.2887632268841225 0.0 16 0.0 0.0 0.0 2.4199319576099643 0.0 17 0.0 0.0 0.0 2.5752364459719064 0.0 18 0.0 0.0 0.0 2.7480538045154757 0.0 19 0.0 0.0 0.0 2.858331547968818 0.0 20 4.4448909090424194E-5 0.0 0.0 2.979677069785676 0.0 21 4.4448909090424194E-5 0.0 0.0 3.135959434147608 0.0 22 4.4448909090424194E-5 0.0 0.0 3.2881969477823105 0.0 23 4.4448909090424194E-5 0.0 0.0 3.447812980326024 0.0 24 4.4448909090424194E-5 0.0 0.0 3.565513691597467 0.0 25 4.4448909090424194E-5 0.0 0.0 3.6769026577780703 0.0 26 4.4448909090424194E-5 0.0 0.0 3.7863358719586944 0.0 27 4.4448909090424194E-5 0.0 0.0 3.922216187048121 0.0 28 4.4448909090424194E-5 0.0 0.0 4.0505846365012665 0.0 29 4.4448909090424194E-5 0.0 0.0 4.200421909045087 0.0 30 4.4448909090424194E-5 0.0 0.0 4.358348883043363 0.0 31 4.4448909090424194E-5 0.0 0.0 4.504185753769045 0.0 32 4.4448909090424194E-5 0.0 0.0 4.639443784131206 0.0 33 4.4448909090424194E-5 0.0 0.0 4.774746263402458 0.0 34 4.4448909090424194E-5 0.0 0.0 4.928450591037144 0.0 35 4.4448909090424194E-5 0.0 0.0 5.093489390489889 0.0 36 4.4448909090424194E-5 0.0 0.0 5.245326863942778 0.0 37 4.4448909090424194E-5 0.0 0.0 5.407432035395555 0.0 38 4.4448909090424194E-5 0.0 0.0 5.589094726848119 0.0 39 4.4448909090424194E-5 0.0 0.0 5.856899404117924 0.0 40 4.4448909090424194E-5 0.0 0.0 6.063986871570211 0.0 41 4.4448909090424194E-5 0.0 0.0 6.242893730659168 0.0 42 8.889781818084839E-5 0.0 0.0 6.448247690656928 0.0 43 8.889781818084839E-5 0.0 0.0 6.631510542836748 0.0 44 8.889781818084839E-5 0.0 0.0 6.817618125198353 0.0 45 8.889781818084839E-5 0.0 0.0 7.000392039378178 0.0 46 8.889781818084839E-5 0.0 0.0 7.196056137194224 0.0 47 8.889781818084839E-5 0.0 0.0 7.397676388828389 0.0 48 8.889781818084839E-5 0.0 0.0 7.589162289189937 0.0 49 8.889781818084839E-5 0.0 0.0 7.797449877187664 0.0 50 8.889781818084839E-5 0.0 0.0 8.0108046408217 0.0 51 8.889781818084839E-5 0.0 0.0 8.233760368819269 0.0 52 1.333467272712726E-4 0.0 0.0 8.499298151725462 0.0 53 1.333467272712726E-4 0.0 0.0 8.728876767177503 0.0 54 1.333467272712726E-4 0.0 0.0 8.96116676608406 0.0 55 1.333467272712726E-4 0.0 0.0 9.187411713354319 0.0 56 1.333467272712726E-4 0.0 0.0 9.427169128988067 0.0 57 1.333467272712726E-4 0.0 0.0 9.681461337894383 0.0 58 1.333467272712726E-4 0.0 0.0 9.914373621528206 0.0 59 1.333467272712726E-4 0.0 0.0 10.168754728252704 0.0 60 1.333467272712726E-4 0.0 0.0 10.43433696006799 0.0 61 1.333467272712726E-4 0.0 0.0 10.731522366246566 0.0 62 1.333467272712726E-4 0.0 0.0 11.057732910061189 0.0 63 1.333467272712726E-4 0.0 0.0 11.348695468967104 0.0 64 1.7779563636169678E-4 0.0 0.0 11.641658228782092 0.0 65 1.7779563636169678E-4 0.0 0.0 11.924797779688094 0.0 66 1.7779563636169678E-4 0.0 0.0 12.20629272095775 0.0 67 1.7779563636169678E-4 0.0 0.0 12.494143856227337 0.0 68 1.7779563636169678E-4 0.0 0.0 12.821643418405582 0.0 69 2.2224454545212097E-4 0.0 0.0 13.273466579309744 0.0 70 2.2224454545212097E-4 0.0 0.0 13.596032312578952 0.0 71 2.2224454545212097E-4 0.0 0.0 13.91015275312098 0.0 72 2.666934545425452E-4 0.0 0.0 14.232096201662923 0.0 73 2.666934545425452E-4 0.0 0.0 14.557328869477557 0.0 74 2.666934545425452E-4 0.0 0.0 14.892562541837536 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 8295 0.0 70.54852 1 GGCGATC 135 0.0 68.14815 8 GATCGCG 30 3.7967657E-6 66.666664 1 GTACGAT 55 9.094947E-12 65.454544 9 TAGGGCA 2245 0.0 64.32072 5 CGTAAGG 265 0.0 63.39623 2 TTACGGG 930 0.0 63.225803 3 CGAGGGC 905 0.0 63.20442 4 GGGCGAT 1405 0.0 63.202847 7 AGGGTAA 2050 0.0 62.634148 6 GTAGGGT 1980 0.0 62.222225 4 TAGGGAC 2040 0.0 62.156864 5 AGTAGGG 4140 0.0 62.125603 3 GTCGAAG 220 0.0 61.81818 1 CCGATCC 3565 0.0 61.71108 80 TAGGGTA 1950 0.0 61.53846 5 AAGGGTC 1545 0.0 60.323624 5 GCACGGG 790 0.0 60.253162 3 TGCGGGC 895 0.0 59.88827 4 CGAGGGA 1470 0.0 59.863945 4 >>END_MODULE