FastQCFastQC Report
Thu 26 May 2016
SRR1547769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547769_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3278020
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336331.0260157046021683No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGC38580.1176929976022111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32910.10039597073843357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150050.073.4421841
GGCGCGA2150.072.558148
GCGCGAG1550.069.6774141
CGTAAGG5200.067.692312
CGGGACC8050.065.590066
TAGGGAC31850.065.55735
GCGGGAT24300.065.185185
GCGCGAC2400.065.09
GGGCGCG2600.064.615397
AGGGATT62550.064.332536
TCGAGCG1000.064.01
TACGGGA11850.063.7974664
ACGGGAT14550.063.5051545
TTAGGGA49450.063.013154
CGAGGGA23450.062.4307024
GCGGGGT23400.062.393165
CGGGATC9750.062.3589746
CGGGATT15850.062.082026
AGGGACC30850.061.847656
TATTGCG5050.061.7821771
Produced by FastQC (version 0.11.5)