##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547769_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3278020
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72658434054704	31.0	31.0	33.0	30.0	34.0
2	32.16857645773973	33.0	31.0	34.0	30.0	34.0
3	32.28404250126601	33.0	31.0	34.0	30.0	34.0
4	35.89747530521473	37.0	35.0	37.0	35.0	37.0
5	35.83606414847988	37.0	35.0	37.0	35.0	37.0
6	35.88808305013392	37.0	35.0	37.0	35.0	37.0
7	36.22339613547202	37.0	35.0	37.0	35.0	37.0
8	36.1987986650478	37.0	36.0	37.0	35.0	37.0
9	38.075698439911896	39.0	39.0	39.0	37.0	39.0
10-11	37.42465497464933	39.0	37.0	39.0	34.5	39.0
12-13	36.763755712289736	39.0	35.0	39.0	33.0	39.0
14-15	37.86726026686841	40.0	36.0	41.0	33.0	41.0
16-17	38.01726667317466	40.0	36.0	41.0	33.0	41.0
18-19	37.87726569697561	40.0	36.0	41.0	33.5	41.0
20-21	37.673935637976584	40.0	35.0	41.0	33.0	41.0
22-23	37.490542766670124	39.0	35.0	41.0	33.0	41.0
24-25	37.39812920604511	39.0	35.0	41.0	33.0	41.0
26-27	37.34063169230206	39.0	35.0	41.0	33.0	41.0
28-29	37.30252164416324	39.0	35.0	41.0	33.0	41.0
30-31	37.08005305031696	39.0	35.0	41.0	32.5	41.0
32-33	36.726268601167774	39.0	35.0	41.0	31.5	41.0
34-35	36.396512986497946	39.0	35.0	41.0	30.5	41.0
36-37	36.15400012812613	39.0	35.0	41.0	30.0	41.0
38-39	36.07315894960982	38.0	35.0	41.0	30.0	41.0
40-41	35.914531180407685	38.0	35.0	41.0	29.0	41.0
42-43	35.84977013563065	38.0	35.0	41.0	29.0	41.0
44-45	35.773301108596044	38.0	35.0	41.0	29.0	41.0
46-47	35.650538739849054	38.0	35.0	40.5	28.0	41.0
48-49	35.51352935613572	38.0	35.0	40.0	28.0	41.0
50-51	35.33409527702699	38.0	35.0	40.0	27.5	41.0
52-53	35.14865177759745	37.0	34.5	40.0	27.0	41.0
54-55	34.93879521784491	37.0	34.0	40.0	26.5	41.0
56-57	34.639199120200615	36.0	34.0	40.0	25.5	41.0
58-59	34.31328179815864	36.0	34.0	40.0	25.0	41.0
60-61	33.976594407599706	35.5	33.5	39.0	24.5	41.0
62-63	33.58613187228876	35.0	33.0	39.0	23.0	40.5
64-65	33.201130865583494	35.0	33.0	38.5	22.0	40.0
66-67	32.87119343384116	35.0	33.0	38.0	20.5	40.0
68-69	32.37676386965302	35.0	32.0	37.0	19.0	40.0
70-71	32.01854183317978	35.0	32.0	36.5	18.0	39.0
72-73	31.70144629990055	35.0	32.0	36.0	17.0	39.0
74-75	31.370046705023157	35.0	31.0	36.0	15.0	38.5
76-77	29.3353126277448	32.5	28.0	34.5	11.0	36.0
78-79	30.374673430912566	34.0	30.0	35.0	11.0	37.0
80-81	30.540117815022484	34.0	31.0	35.0	10.5	36.5
82-83	30.329821355574403	34.0	31.0	35.0	10.0	36.0
84-85	30.085718055411498	34.0	30.0	35.0	10.0	36.0
86	29.662005417904712	34.0	29.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	12.0
11	24.0
12	51.0
13	161.0
14	602.0
15	1781.0
16	4671.0
17	11170.0
18	22170.0
19	30049.0
20	31925.0
21	30439.0
22	27088.0
23	25897.0
24	28335.0
25	32754.0
26	39466.0
27	47407.0
28	56981.0
29	69803.0
30	85314.0
31	103557.0
32	122282.0
33	153662.0
34	334585.0
35	326090.0
36	279611.0
37	446798.0
38	641603.0
39	322450.0
40	1267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.49314525231695	24.105313573437623	32.55492644950305	14.846614724742373
2	30.76775004423402	25.494292286197158	31.558776334494603	12.17918133507422
3	27.530246917346446	24.604730904631456	34.900580228308556	12.964441949713546
4	25.352346843521396	26.97042727012038	33.64354091799318	14.033684968365048
5	24.60860519459918	30.767261944710526	31.51936229797256	13.104770562717738
6	22.457550594566232	38.81696877993423	28.454158302877957	10.271322322621582
7	87.70876931806396	3.4568428502571678	7.450991757219297	1.3833960744595823
8	88.87788970171017	2.609136002830977	7.010329406165917	1.5026448892929267
9	84.6836504963362	5.14557568288174	8.258948999701039	1.9118248210810183
10-11	38.21631655694596	28.769318063953243	21.54341645261469	11.47094892648611
12-13	26.657799525323213	27.187036686780434	29.945988737103495	16.209175050792858
14-15	15.60260462108224	32.680230749049734	35.74433652021647	15.972828109651557
16-17	20.243226093800526	27.65166167381529	33.26180133129145	18.84331090109273
18-19	22.219891885955544	29.871080713357458	31.107162250382853	16.801865150304145
20-21	23.72186563840367	29.505738220023066	30.977022714931575	15.795373426641692
22-23	20.029499514951098	26.999362419997436	31.928389698659558	21.04274836639191
24-25	18.247585432669723	29.524118217704594	34.73673131951605	17.49156503010964
26-27	17.26206673540735	35.646106491113535	30.041778268588963	17.050048504890146
28-29	16.303561296148285	30.617750959420626	35.15995936571467	17.91872837871642
30-31	20.743406080499813	30.8607177503493	31.134129749055834	17.261746420095054
32-33	22.895574157570728	30.269644480509577	29.555798927401295	17.278982434518397
34-35	19.085896364268677	30.384759702503338	30.523761294928036	20.00558263829995
36-37	21.330391516830282	30.854463975204542	30.698714467880002	17.116430040085174
38-39	18.978453456659814	32.73648421913229	29.428999823063922	18.856062501143985
40-41	19.97644919799147	29.336840531784432	29.743107119541673	20.943603150682424
42-43	19.86825888798726	29.12250382853064	29.66032849097931	21.34890879250279
44-45	18.848130883887222	32.904115899231854	28.322569721966307	19.925183494914613
46-47	20.355595755974644	32.104517361089925	28.309711350144294	19.230175532791137
48-49	20.00128126124917	29.332051055210158	31.162775089840817	19.503892593699856
50-51	18.728577006851697	32.0864729318308	29.143995460674432	20.040954600643072
52-53	20.729754546952122	29.157021616707645	30.57380674919616	19.539417087144066
54-55	21.001839525079163	29.17329668519411	30.855135112049346	18.969728677677377
56-57	21.393356355360858	28.054496311797976	29.88624230480595	20.665905028035215
58-59	22.04190334409186	30.016854686670612	29.11936168784815	18.821880281389376
60-61	22.104715651521346	31.04613455683614	27.574175874460806	19.274973917181715
62-63	21.168220450149786	29.269681087973836	31.201334952196753	18.36076350967962
64-65	18.55366959323006	32.639993044581786	30.257564017303128	18.54877334488502
66-67	17.921367166765304	31.813732069969063	27.073828103550312	23.191072659715317
68-69	17.64879408911477	33.518861995960975	26.87263958121061	21.959704333713645
70-71	18.73611204324562	32.18609099395367	28.859113123165812	20.218683839634902
72-73	20.816483731032758	32.155844076607224	28.208018865046586	18.81965332731344
74-75	20.324601436232847	33.15829372609075	26.13077406483182	20.386330772844584
76-77	23.388432651417624	30.93298698604646	27.290010433127314	18.388569929408607
78-79	18.33562638421974	32.26278363158248	29.639004643046718	19.76258534115106
80-81	16.718644181548616	30.72064844021696	31.139773399796216	21.420933978438203
82-83	17.921138370113667	29.872728049249243	31.046043038175487	21.160090542461607
84-85	17.14435848469503	29.485970189321602	30.106787023874165	23.2628843021092
86	17.322530063880027	29.817908371516953	31.1015796120829	21.75798195252012
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2445.0
1	2976.0
2	11854.0
3	12317.0
4	5155.0
5	7676.5
6	11478.5
7	13481.0
8	14800.0
9	15892.0
10	15265.5
11	13870.0
12	12233.0
13	11759.0
14	11926.0
15	11612.5
16	11397.0
17	12010.5
18	13139.5
19	13994.0
20	15222.5
21	16210.0
22	16111.0
23	17488.5
24	21368.5
25	23496.0
26	26932.5
27	35079.0
28	42938.0
29	46363.0
30	52238.5
31	64772.0
32	70193.0
33	71090.5
34	81501.5
35	93416.0
36	97054.0
37	97303.0
38	108097.5
39	126060.5
40	138795.5
41	156036.5
42	174425.0
43	180890.0
44	180396.5
45	175371.0
46	174144.5
47	183945.5
48	175639.5
49	145565.5
50	130293.0
51	116175.0
52	93583.5
53	79066.0
54	67775.0
55	58666.5
56	50990.5
57	47176.0
58	41813.0
59	30749.0
60	21760.0
61	16794.0
62	12753.5
63	8142.0
64	5893.0
65	4606.0
66	2729.5
67	1766.0
68	1257.5
69	855.0
70	501.0
71	415.0
72	376.5
73	314.5
74	249.0
75	181.0
76	129.5
77	106.5
78	108.5
79	108.0
80	102.0
81	82.0
82	44.5
83	16.5
84	11.0
85	10.0
86	10.0
87	9.0
88	5.0
89	3.0
90	4.0
91	2.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3278020.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.637064979909493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1608269737052	14.38051346212497
2	9.581508832626563	3.57142405438476
3	3.2965015181887325	1.8431133900256107
4	1.6394854049841854	1.2222078410521395
5	1.0494575080453659	0.9779403885547686
6	0.7655697265192968	0.8560783643875004
7	0.598709782137736	0.7810735179665909
8	0.4514847457973939	0.6731480435891158
9	0.374060885429566	0.6274257325373972
>10	2.9681306846364	11.602252484972649
>50	0.7688806975635437	10.360499142233493
>100	1.3348040223261515	50.31150402895872
>500	0.007934413529834369	0.9333017290098164
>1k	0.0024795042280732404	0.8233819214802894
>5k	0.0	0.0
>10k+	1.6530028187154936E-4	1.0361358987221376
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33633	1.0260157046021683	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGC	3858	0.1176929976022111	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3291	0.10039597073843357	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0364244269406532	0.0
2	0.0	0.0	0.0	0.17321431839952167	0.0
3	0.0	0.0	0.0	0.2839518977919598	0.0
4	0.0	0.0	0.0	0.4506378850647647	0.0
5	0.0	0.0	0.0	0.8609465470009335	0.0
6	0.0	0.0	0.0	1.2178693235550728	0.0
7	0.0	0.0	0.0	1.4749452413347082	0.0
8	0.0	0.0	0.0	1.9521540442096144	0.0
9	0.0	0.0	0.0	2.1279003788872553	0.0
10	0.0	0.0	0.0	2.4246038767304654	0.0
11	0.0	0.0	0.0	3.02673565139932	0.0
12	0.0	0.0	0.0	3.4439692253250436	0.0
13	0.0	0.0	0.0	3.5939072976980007	0.0
14	0.0	0.0	0.0	3.651838609892557	0.0
15	0.0	0.0	0.0	3.7376831135868605	0.0
16	0.0	0.0	0.0	3.9302993880452224	0.0
17	0.0	0.0	0.0	4.187527836925949	0.0
18	3.0506220218302512E-5	0.0	0.0	4.46464634138901	0.0
19	3.0506220218302512E-5	0.0	0.0	4.669556622595347	0.0
20	3.0506220218302512E-5	0.0	0.0	4.858725694169041	0.0
21	3.0506220218302512E-5	0.0	0.0	5.107595438709953	0.0
22	3.0506220218302512E-5	0.0	0.0	5.3857511546604355	0.0
23	3.0506220218302512E-5	0.0	0.0	5.674309491705359	0.0
24	3.0506220218302512E-5	0.0	0.0	5.879189266691478	0.0
25	3.0506220218302512E-5	0.0	0.0	6.062958737286532	0.0
26	3.0506220218302512E-5	0.0	0.0	6.233305470985534	0.0
27	3.0506220218302512E-5	0.0	0.0	6.420979737768531	0.0
28	3.0506220218302512E-5	0.0	0.0	6.617744858176582	0.0
29	3.0506220218302512E-5	0.0	0.0	6.839159004521022	0.0
30	6.1012440436605025E-5	0.0	0.0	7.074758543266972	0.0
31	6.1012440436605025E-5	0.0	0.0	7.307002397788909	0.0
32	6.1012440436605025E-5	0.0	0.0	7.50843496989036	0.0
33	6.1012440436605025E-5	0.0	0.0	7.717555109486824	0.0
34	6.1012440436605025E-5	0.0	0.0	7.9267362615237245	0.0
35	6.1012440436605025E-5	0.0	0.0	8.16117656390138	0.0
36	6.1012440436605025E-5	0.0	0.0	8.380211225068791	0.0
37	6.1012440436605025E-5	0.0	0.0	8.6212408710136	0.0
38	9.151866065490753E-5	0.0	0.0	8.877981220370833	0.0
39	9.151866065490753E-5	0.0	0.0	9.21626469637159	0.0
40	9.151866065490753E-5	0.0	0.0	9.469801892605902	0.0
41	9.151866065490753E-5	0.0	0.0	9.730752100353262	0.0
42	9.151866065490753E-5	0.0	0.0	9.996003685151402	0.0
43	9.151866065490753E-5	0.0	0.0	10.241792301450266	0.0
44	9.151866065490753E-5	0.0	0.0	10.482089798109834	0.0
45	9.151866065490753E-5	0.0	0.0	10.731325617293367	0.0
46	9.151866065490753E-5	0.0	0.0	10.990750514029811	0.0
47	9.151866065490753E-5	0.0	0.0	11.261706762008773	0.0
48	9.151866065490753E-5	0.0	0.0	11.53458490186149	0.0
49	9.151866065490753E-5	0.0	0.0	11.824363487715145	0.0
50	9.151866065490753E-5	0.0	0.0	12.11578940946059	0.0
51	9.151866065490753E-5	0.0	0.0	12.40440875894595	0.0
52	9.151866065490753E-5	0.0	0.0	12.732289613852265	0.0
53	9.151866065490753E-5	0.0	0.0	13.011055454207114	0.0
54	9.151866065490753E-5	0.0	0.0	13.311023117613681	0.0
55	9.151866065490753E-5	0.0	0.0	13.602479545579344	0.0
56	9.151866065490753E-5	0.0	0.0	13.902233665444385	0.0
57	9.151866065490753E-5	0.0	0.0	14.203604614980994	0.0
58	9.151866065490753E-5	0.0	0.0	14.486092214202476	0.0
59	9.151866065490753E-5	0.0	0.0	14.791001885284409	0.0
60	9.151866065490753E-5	0.0	0.0	15.122085893313647	0.0
61	1.2202488087321005E-4	0.0	0.0	15.452590283158736	0.0
62	1.2202488087321005E-4	0.0	0.0	15.805455732423841	0.0
63	1.2202488087321005E-4	0.0	0.0	16.14712539886883	0.0
64	1.2202488087321005E-4	0.0	0.0	16.481534584901862	0.0
65	1.2202488087321005E-4	0.0	0.0	16.793430180413786	0.0
66	1.2202488087321005E-4	0.0	0.0	17.09833985149572	0.0
67	1.2202488087321005E-4	0.0	0.0	17.403188510137216	0.0
68	1.2202488087321005E-4	0.0	0.0	17.76026381779245	0.0
69	1.2202488087321005E-4	0.0	0.0	18.21257954496922	0.0
70	1.2202488087321005E-4	0.0	0.0	18.572735980866497	0.0
71	1.5253110109151257E-4	0.0	0.0	18.904338594639448	0.0
72	1.8303732130981506E-4	0.0	0.0	19.24359826968719	0.0
73	1.8303732130981506E-4	0.0	0.0	19.586335653839818	0.0
74	1.8303732130981506E-4	0.0	0.0	19.94231273756719	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15005	0.0	73.442184	1
GGCGCGA	215	0.0	72.55814	8
GCGCGAG	155	0.0	69.677414	1
CGTAAGG	520	0.0	67.69231	2
CGGGACC	805	0.0	65.59006	6
TAGGGAC	3185	0.0	65.5573	5
GCGGGAT	2430	0.0	65.18518	5
GCGCGAC	240	0.0	65.0	9
GGGCGCG	260	0.0	64.61539	7
AGGGATT	6255	0.0	64.33253	6
TCGAGCG	100	0.0	64.0	1
TACGGGA	1185	0.0	63.797466	4
ACGGGAT	1455	0.0	63.505154	5
TTAGGGA	4945	0.0	63.01315	4
CGAGGGA	2345	0.0	62.430702	4
GCGGGGT	2340	0.0	62.39316	5
CGGGATC	975	0.0	62.358974	6
CGGGATT	1585	0.0	62.08202	6
AGGGACC	3085	0.0	61.84765	6
TATTGCG	505	0.0	61.782177	1
>>END_MODULE