FastQCFastQC Report
Thu 26 May 2016
SRR1547768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547768_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2559107
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142710.5576554634096972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG950.071.578941
CGTTTTT74850.069.846351
TACGGGA13650.069.450554
CGTAAGG5450.067.522942
CGTTAGG5050.066.534652
TATTACG1150.066.086951
GTACGGG10250.065.951223
GGGCGAT16800.064.285727
TAGGGAC29900.064.080265
ACGGGAT18100.063.6464085
AGGGATT53750.063.032566
ATTGCGG7950.062.893082
TTGCGGG21650.062.8175543
ATAGGGA40200.062.487564
CGGGATC12700.062.0472456
TAGGGAT50200.061.8326725
CTACGGG6800.061.764713
TAGTCGG1300.061.538462
GCGGGAT24550.061.4256635
CGGGATA10600.061.1320726
Produced by FastQC (version 0.11.5)