##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547768_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2559107
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85650814913171	31.0	31.0	33.0	31.0	34.0
2	32.31298105159339	33.0	31.0	34.0	31.0	34.0
3	32.480009237597336	34.0	31.0	34.0	31.0	34.0
4	36.04876662054381	37.0	35.0	37.0	35.0	37.0
5	36.00817394505193	37.0	35.0	37.0	35.0	37.0
6	36.070854403508726	37.0	35.0	37.0	35.0	37.0
7	36.28314095502845	37.0	36.0	37.0	35.0	37.0
8	36.24646136328024	37.0	37.0	37.0	35.0	37.0
9	38.1505845593795	39.0	39.0	39.0	37.0	39.0
10-11	37.6336050036204	39.0	37.5	39.0	35.0	39.0
12-13	36.77885821108691	39.0	35.0	39.0	33.0	39.0
14-15	37.80618571243797	40.0	35.0	41.0	33.0	41.0
16-17	37.9985782149789	40.0	35.0	41.0	34.0	41.0
18-19	37.897065460725166	40.0	36.0	41.0	34.0	41.0
20-21	37.7235871731819	40.0	35.0	41.0	34.0	41.0
22-23	37.530620837659384	39.0	35.0	41.0	33.0	41.0
24-25	37.47001649403484	39.0	35.0	41.0	33.0	41.0
26-27	37.456932046999206	39.0	35.0	41.0	33.0	41.0
28-29	37.470389475703826	39.0	35.0	41.0	33.0	41.0
30-31	37.31284350361278	39.0	35.0	41.0	33.0	41.0
32-33	37.005779750514534	39.0	35.0	41.0	32.5	41.0
34-35	36.76358022544583	39.0	35.0	41.0	32.0	41.0
36-37	36.57727891018234	39.0	35.0	41.0	31.0	41.0
38-39	36.489632907103925	39.0	35.0	41.0	31.0	41.0
40-41	36.34160783429532	38.5	35.0	41.0	31.0	41.0
42-43	36.298234110570604	38.0	35.0	41.0	31.0	41.0
44-45	36.188735953596314	38.0	35.0	41.0	31.0	41.0
46-47	36.104043324487805	38.0	35.0	40.5	31.0	41.0
48-49	36.01299046894093	38.0	35.0	40.0	30.5	41.0
50-51	35.83190386333983	37.5	35.0	40.0	30.0	41.0
52-53	35.65733320255855	37.0	35.0	40.0	30.0	41.0
54-55	35.46582890047192	37.0	35.0	40.0	29.5	41.0
56-57	35.1627638078439	36.0	34.5	40.0	28.5	41.0
58-59	34.855903836768064	36.0	34.0	40.0	28.0	41.0
60-61	34.5631935280549	35.0	34.0	39.0	27.5	41.0
62-63	34.17746932816799	35.0	33.5	39.0	26.5	40.5
64-65	33.907588662764	35.0	33.5	38.5	26.0	40.0
66-67	33.65551381790601	35.0	34.0	37.5	26.0	40.0
68-69	33.260637988173215	35.0	33.0	37.0	25.5	39.5
70-71	33.04124817758695	35.0	33.0	36.5	25.0	39.0
72-73	32.78946464528447	35.0	33.0	36.0	25.0	39.0
74-75	32.50328923331459	35.0	33.0	36.0	24.5	38.0
76-77	30.466205008231384	33.0	30.0	34.5	20.0	36.0
78-79	31.584298155567545	34.0	31.5	35.0	23.0	37.0
80-81	31.87627559144655	35.0	32.5	35.0	23.5	36.5
82-83	31.74824167180192	35.0	32.5	35.0	23.0	36.0
84-85	31.567411405619225	35.0	32.0	35.0	22.0	36.0
86	31.164631256137394	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	22.0
10	13.0
11	31.0
12	63.0
13	119.0
14	333.0
15	896.0
16	2223.0
17	4903.0
18	9754.0
19	15058.0
20	18394.0
21	19231.0
22	17527.0
23	16304.0
24	16804.0
25	18193.0
26	22163.0
27	27011.0
28	33157.0
29	42010.0
30	52960.0
31	66713.0
32	85420.0
33	118677.0
34	310379.0
35	278290.0
36	226122.0
37	386172.0
38	524786.0
39	244591.0
40	782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.137189652484242	24.288120817144417	30.368562158596728	14.206127371774608
2	29.076392663534584	26.484121218847044	31.74591761891941	12.693568498698959
3	29.07670527258141	25.364824526680597	32.5966831398609	12.961787060877095
4	25.749333654278622	27.29491185792544	32.50700341955221	14.448751068243727
5	24.574470704038557	30.85560705355423	30.656592319117564	13.913329923289647
6	22.280740899071432	40.62897721744343	26.075033204942194	11.015248678542946
7	89.22202158799925	3.411697908684553	5.911124466464279	1.4551560368519174
8	90.30271106288248	2.669915716693362	5.480349199935759	1.547024020488397
9	86.3793112206719	5.179072231055599	6.479056952288436	1.9625595959840678
10-11	42.33836256162794	27.56883553520818	19.08659934891351	11.006202554250368
12-13	31.11319690814022	25.997076323889544	26.793037571309053	16.096689196661178
14-15	16.126484746436944	32.09015879367295	34.998360756310696	16.784995703579416
16-17	22.13162638373464	25.783837877822226	32.37910724326885	19.705428495174292
18-19	24.22198446567494	27.670765622539424	29.809343649952897	18.297906261832743
20-21	27.081458493138427	28.141652537389017	28.74262389184977	16.03426507762278
22-23	22.289259495597488	24.465077075714305	31.00395958433938	22.24170384434883
24-25	19.341844635648293	26.685519597265767	34.93261516614975	19.040020600936185
26-27	18.19337761179974	34.33051060389425	29.044799611739563	18.431312172566447
28-29	16.40929824348884	29.239437819520635	35.5902273722826	18.761036564707922
30-31	22.955546602779798	28.799792271288382	30.06775410328681	18.176907022645008
32-33	26.63477924135255	27.441994414457856	28.414482083007865	17.508744261181732
34-35	19.774808165504606	28.84144742677817	30.283845106906433	21.09989930081079
36-37	23.726440512256815	28.987514003908394	29.40355756910516	17.88248791472963
38-39	19.91085171507092	31.259478403990144	28.05420015653898	20.775469724399954
40-41	20.99077920540251	27.108069338249628	28.409226343408072	23.49192511293979
42-43	21.154625422071057	25.74784876130619	28.721581395385186	24.375944421237563
44-45	19.24749531770262	31.513590482930177	28.297742142083155	20.941172057284042
46-47	21.165371358055758	30.326985155368654	27.822693619297667	20.684949867277922
48-49	21.469579036749927	26.961514309483736	31.52021388710984	20.048692766656494
50-51	19.60158367743123	30.921294029518894	28.47688666398083	21.000235629069046
52-53	21.85430308306765	27.04578198566922	30.36860123472758	20.73131369653555
54-55	22.609683768595843	26.818397980232948	30.255260917187126	20.316657333984082
56-57	23.106907995640665	25.570423589166065	28.856882498465286	22.465785916727985
58-59	24.619115183538636	27.744228748543925	27.810150181293707	19.826505886623732
60-61	24.402887413461023	28.5476926130873	27.26277174029847	19.786648233153205
62-63	23.028775271999177	25.722664194971134	31.06554356656443	20.183016966465257
64-65	20.26744094717415	29.628851001540774	30.53758596260336	19.566122088681716
66-67	19.456826150684595	28.85086477431385	25.867695254633745	25.82461382036781
68-69	18.40153616085611	31.366136703154655	25.996959095496987	24.23536804049225
70-71	19.311443405844305	30.522326733505086	29.03663270039119	21.12959716025942
72-73	22.875753143577036	29.84351572638424	27.32208930693402	19.9586418231047
74-75	21.95746406852078	31.47027459187912	25.049401998431485	21.52285934116862
76-77	25.48568699941034	28.00162322247565	26.441391469758784	20.07129830835522
78-79	19.74772840682316	28.490328852994423	30.44225974138635	21.319682998796065
80-81	17.314614043101752	28.46229172910707	30.749593510548795	23.47350071724238
82-83	18.909408633558503	27.181219855207306	31.566011112470093	22.3433603987641
84-85	17.48623640981014	26.860678353816393	29.91354015287364	25.73954508349983
86	18.185093472058806	27.283462551585373	31.440733036953905	23.09071093940191
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1591.0
1	2032.0
2	5824.5
3	6109.5
4	3291.0
5	4018.0
6	4911.0
7	5325.0
8	5461.5
9	5620.0
10	5673.0
11	5425.0
12	5241.5
13	5419.5
14	5502.0
15	5406.0
16	5465.0
17	5585.5
18	5762.5
19	6295.0
20	6450.0
21	6284.0
22	7070.5
23	8442.0
24	10110.5
25	14361.5
26	18633.0
27	21061.5
28	25414.5
29	28443.0
30	33746.5
31	41500.0
32	44777.5
33	50459.0
34	58077.5
35	63441.5
36	66041.0
37	69219.0
38	73455.0
39	78740.5
40	95111.0
41	109934.0
42	118699.0
43	124784.0
44	128800.0
45	138044.0
46	145518.5
47	149099.5
48	145283.0
49	130147.5
50	120163.0
51	107290.0
52	88403.0
53	78176.0
54	71386.0
55	67685.5
56	61132.5
57	55703.0
58	50952.5
59	40001.0
60	30789.5
61	24409.5
62	18604.5
63	13339.5
64	10510.0
65	8936.5
66	6883.5
67	5790.0
68	4226.0
69	2450.0
70	1515.0
71	1406.0
72	1236.5
73	755.5
74	367.5
75	246.0
76	156.0
77	123.5
78	118.0
79	100.0
80	74.5
81	41.0
82	27.5
83	17.5
84	11.0
85	7.0
86	5.0
87	4.5
88	4.0
89	3.5
90	4.0
91	3.5
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2559107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.490229084149398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16758304183537	11.61646643398765
2	8.591159529360583	2.4897573935781594
3	2.4846798338583724	1.0801073998012227
4	1.183443217263849	0.6859345330494384
5	0.7112661487911727	0.5153204717892391
6	0.480096819878394	0.4174027741565724
7	0.3734090609019682	0.37875479841966286
8	0.282654937697207	0.3276587839198804
9	0.24212426223172165	0.3157592423911477
>10	2.293035690651376	7.60628058958648
>50	1.0578070521445122	11.237643954929254
>100	2.114793080796308	60.813494805176916
>500	0.014956103824313212	1.3960933015380426
>1k	0.002719291604420584	0.5570030456532915
>5k	0.0	0.0
>10k+	2.719291604420584E-4	0.5623224720230704
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14271	0.5576554634096972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03712232431078497	0.0
2	0.0	0.0	0.0	0.12086247272974518	0.0
3	0.0	0.0	0.0	0.1788905270471301	0.0
4	0.0	0.0	0.0	0.27193079460921327	0.0
5	0.0	0.0	0.0	0.4503524080861019	0.0
6	0.0	0.0	0.0	0.6086498141734598	0.0
7	0.0	0.0	0.0	0.701103939772741	0.0
8	0.0	0.0	0.0	0.8952732339835732	0.0
9	0.0	0.0	0.0	0.9589673272747095	0.0
10	0.0	0.0	0.0	1.0734213145444875	0.0
11	0.0	0.0	0.0	1.3052990750289066	0.0
12	0.0	0.0	0.0	1.4746159500169396	0.0
13	0.0	0.0	0.0	1.537802053606981	0.0
14	0.0	0.0	0.0	1.5668356188311001	0.0
15	0.0	0.0	0.0	1.6099756672933176	0.0
16	0.0	0.0	0.0	1.6942238054133727	0.0
17	0.0	0.0	0.0	1.802034850438063	0.0
18	0.0	0.0	0.0	1.921685963111351	0.0
19	0.0	0.0	0.0	2.009919866578459	0.0
20	0.0	0.0	0.0	2.101279860513843	0.0
21	0.0	0.0	0.0	2.2134283560632673	0.0
22	0.0	0.0	0.0	2.3343689810547197	0.0
23	0.0	0.0	0.0	2.459959665617733	0.0
24	0.0	0.0	0.0	2.549639385926419	0.0
25	0.0	0.0	0.0	2.6322854026814824	0.0
26	0.0	0.0	0.0	2.71520495235252	0.0
27	0.0	0.0	0.0	2.820632353395149	0.0
28	0.0	0.0	0.0	2.9194949644543975	0.0
29	0.0	0.0	0.0	3.0418814062874278	0.0
30	0.0	0.0	0.0	3.1716141607209076	0.0
31	0.0	0.0	0.0	3.284739559541668	0.0
32	0.0	0.0	0.0	3.395676695034635	0.0
33	0.0	0.0	0.0	3.5061449169573606	0.0
34	0.0	0.0	0.0	3.62411575600395	0.0
35	0.0	0.0	0.0	3.7550207943630336	0.0
36	0.0	0.0	0.0	3.8797908801781245	0.0
37	0.0	0.0	0.0	4.017260708520589	0.0
38	0.0	0.0	0.0	4.166375223857385	0.0
39	0.0	0.0	0.0	4.418299039469628	0.0
40	0.0	0.0	0.0	4.573431278957855	0.0
41	0.0	0.0	0.0	4.7357144503922655	0.0
42	0.0	0.0	0.0	4.898232078611797	0.0
43	0.0	0.0	0.0	5.03624897278621	0.0
44	0.0	0.0	0.0	5.182628158963263	0.0
45	0.0	0.0	0.0	5.3252169604475315	0.0
46	0.0	0.0	0.0	5.487617360274502	0.0
47	0.0	0.0	0.0	5.6673675622004085	0.0
48	0.0	0.0	0.0	5.82582127281118	0.0
49	0.0	0.0	0.0	6.002640764923076	3.907613085345787E-5
50	0.0	0.0	0.0	6.1789131912030255	3.907613085345787E-5
51	0.0	0.0	0.0	6.362141168774889	3.907613085345787E-5
52	0.0	0.0	0.0	6.584601581723625	3.907613085345787E-5
53	0.0	0.0	0.0	6.758216831105538	3.907613085345787E-5
54	0.0	0.0	0.0	6.955629444177207	3.907613085345787E-5
55	0.0	0.0	0.0	7.1392872591884595	3.907613085345787E-5
56	3.907613085345787E-5	0.0	0.0	7.324078281994462	3.907613085345787E-5
57	3.907613085345787E-5	0.0	0.0	7.53051748129328	3.907613085345787E-5
58	3.907613085345787E-5	0.0	0.0	7.718161061651584	3.907613085345787E-5
59	3.907613085345787E-5	0.0	0.0	7.920067429771401	3.907613085345787E-5
60	7.815226170691573E-5	0.0	0.0	8.134868921072858	3.907613085345787E-5
61	7.815226170691573E-5	0.0	0.0	8.359478521218534	3.907613085345787E-5
62	7.815226170691573E-5	0.0	0.0	8.630471488687265	3.907613085345787E-5
63	7.815226170691573E-5	0.0	0.0	8.89337569706933	3.907613085345787E-5
64	1.172283925603736E-4	0.0	0.0	9.129629984209336	3.907613085345787E-5
65	1.172283925603736E-4	0.0	0.0	9.352012244896365	3.907613085345787E-5
66	1.172283925603736E-4	0.0	0.0	9.559506499728226	3.907613085345787E-5
67	1.5630452341383147E-4	0.0	0.0	9.79505741651287	3.907613085345787E-5
68	1.5630452341383147E-4	0.0	0.0	10.06651929755184	3.907613085345787E-5
69	1.5630452341383147E-4	0.0	0.0	10.46146175208774	3.907613085345787E-5
70	1.5630452341383147E-4	0.0	0.0	10.715378450373509	3.907613085345787E-5
71	1.5630452341383147E-4	0.0	0.0	10.966129982060147	3.907613085345787E-5
72	1.5630452341383147E-4	0.0	0.0	11.205393131275871	3.907613085345787E-5
73	1.5630452341383147E-4	0.0	0.0	11.455714825523122	3.907613085345787E-5
74	1.5630452341383147E-4	0.0	0.0	11.743432376997132	3.907613085345787E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	95	0.0	71.57894	1
CGTTTTT	7485	0.0	69.84635	1
TACGGGA	1365	0.0	69.45055	4
CGTAAGG	545	0.0	67.52294	2
CGTTAGG	505	0.0	66.53465	2
TATTACG	115	0.0	66.08695	1
GTACGGG	1025	0.0	65.95122	3
GGGCGAT	1680	0.0	64.28572	7
TAGGGAC	2990	0.0	64.08026	5
ACGGGAT	1810	0.0	63.646408	5
AGGGATT	5375	0.0	63.03256	6
ATTGCGG	795	0.0	62.89308	2
TTGCGGG	2165	0.0	62.817554	3
ATAGGGA	4020	0.0	62.48756	4
CGGGATC	1270	0.0	62.047245	6
TAGGGAT	5020	0.0	61.832672	5
CTACGGG	680	0.0	61.76471	3
TAGTCGG	130	0.0	61.53846	2
GCGGGAT	2455	0.0	61.425663	5
CGGGATA	1060	0.0	61.132072	6
>>END_MODULE