FastQCFastQC Report
Thu 26 May 2016
SRR1547767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547767_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2632197
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180830.6869926529055387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA12000.068.3333364
CGAGGGA16150.067.368424
CTACGGG4650.067.096773
TAGGGAC29650.066.9139945
CGAACGG1200.066.666672
CGTTTTT84250.066.658751
CGAAACG3800.066.3157980
AGGGATT57300.065.20076
ACGGGAT12900.064.186045
TATTGCG3250.064.01
TATAGCG1250.064.01
TTAGGGA44650.063.9641654
CGGGATA6500.063.3846176
TACGCGG2600.063.076922
ATTGCGG6500.062.769232
TCGATAG2550.062.74511
TTGCGGG21750.062.3448263
ATAGGGA37950.062.292494
CGTAAGG5600.062.1428572
ACGGGAC8950.061.229055
Produced by FastQC (version 0.11.5)