##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547767_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2632197
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80195479289734	31.0	31.0	33.0	30.0	34.0
2	32.24239941007455	33.0	31.0	34.0	31.0	34.0
3	32.39386261742567	34.0	31.0	34.0	31.0	34.0
4	35.979223819493754	37.0	35.0	37.0	35.0	37.0
5	35.92429555994479	37.0	35.0	37.0	35.0	37.0
6	35.9905288244003	37.0	35.0	37.0	35.0	37.0
7	36.25838605545102	37.0	35.0	37.0	35.0	37.0
8	36.213869630578564	37.0	36.0	37.0	35.0	37.0
9	38.092475981091084	39.0	39.0	39.0	37.0	39.0
10-11	37.53124196251268	39.0	37.5	39.0	35.0	39.0
12-13	36.86056058874013	39.0	35.0	39.0	33.0	39.0
14-15	37.91667341008291	40.0	36.0	41.0	33.0	41.0
16-17	38.100923677065204	40.0	36.0	41.0	33.5	41.0
18-19	37.97781700989705	40.0	36.0	41.0	34.0	41.0
20-21	37.80189514690579	40.0	35.0	41.0	33.5	41.0
22-23	37.637495597783904	39.5	35.0	41.0	33.0	41.0
24-25	37.55879024252364	39.0	35.0	41.0	33.0	41.0
26-27	37.54215205016949	39.5	35.0	41.0	33.0	41.0
28-29	37.50895639650072	40.0	35.0	41.0	33.0	41.0
30-31	37.34595852818007	39.5	35.0	41.0	33.0	41.0
32-33	37.06023219386695	39.0	35.0	41.0	32.0	41.0
34-35	36.81406976757439	39.0	35.0	41.0	31.0	41.0
36-37	36.64164536317001	39.0	35.0	41.0	31.0	41.0
38-39	36.53593481035044	39.0	35.0	41.0	31.0	41.0
40-41	36.38820403640001	39.0	35.0	41.0	30.0	41.0
42-43	36.359516024066586	39.0	35.0	41.0	30.0	41.0
44-45	36.2515581090625	39.0	35.0	41.0	30.0	41.0
46-47	36.18045704785774	38.0	35.0	41.0	30.0	41.0
48-49	36.076939909892765	38.0	35.0	40.5	30.0	41.0
50-51	35.90321184166687	38.0	35.0	40.0	30.0	41.0
52-53	35.71037502132249	38.0	35.0	40.0	29.0	41.0
54-55	35.51584550852387	37.0	35.0	40.0	29.0	41.0
56-57	35.21627218631433	37.0	34.5	40.0	28.0	41.0
58-59	34.92003733003267	36.5	34.0	40.0	27.0	41.0
60-61	34.629931004404305	36.0	34.0	39.5	26.5	41.0
62-63	34.312245625992276	35.5	34.0	39.0	26.0	41.0
64-65	34.010131650480574	35.0	33.5	39.0	26.0	40.0
66-67	33.74029185505492	35.0	34.0	38.5	26.0	40.0
68-69	33.30599476406971	35.0	33.0	37.5	24.5	40.0
70-71	33.051799504368404	35.0	33.0	37.0	24.5	39.5
72-73	32.75014484098264	35.0	33.0	36.5	24.0	39.0
74-75	32.438032563672095	35.0	33.0	36.0	23.5	39.0
76-77	30.35748768044337	33.0	29.5	34.5	19.5	36.0
78-79	31.512305879841062	34.0	31.5	35.0	20.0	37.0
80-81	31.694237171457914	35.0	32.0	35.0	20.0	37.0
82-83	31.52061585815955	35.0	32.0	35.0	20.0	36.0
84-85	31.302245614594955	35.0	32.0	35.0	19.5	36.0
86	30.87791339326046	34.0	31.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	6.0
10	15.0
11	13.0
12	41.0
13	115.0
14	381.0
15	1044.0
16	2606.0
17	5658.0
18	11138.0
19	16675.0
20	19591.0
21	20472.0
22	18372.0
23	17356.0
24	18238.0
25	20749.0
26	24754.0
27	29889.0
28	37232.0
29	46174.0
30	57321.0
31	71563.0
32	89205.0
33	120419.0
34	272903.0
35	253831.0
36	232133.0
37	397771.0
38	551614.0
39	293783.0
40	1131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.864256360751114	23.518376474101295	30.547371644295623	14.069995520851972
2	30.51443338017633	24.9713072387819	31.296327744465934	13.217931636575834
3	29.74245468709219	25.396883287990985	32.38716555029886	12.473496474617972
4	26.563399320035696	26.25551962866001	32.17988623191957	15.00119481938472
5	24.371428126390235	31.787134473597533	30.264832001556112	13.576605398456119
6	23.447029230714875	40.3775629255713	25.76942379312794	10.40598405058588
7	88.73237831362925	3.2655990414091347	6.418060654274738	1.5839619906868674
8	89.26543112084696	2.7268475725791044	6.4084869027660165	1.5992344038079216
9	84.98691397338422	5.3725082127211605	7.3090653929018226	2.3315124209928055
10-11	38.03546998951826	29.88083718657836	20.23583341216482	11.84785941173856
12-13	28.82856032432223	24.463119591732685	29.201917637623627	17.506402446321456
14-15	16.388192069210625	31.18396913300942	35.246829929522754	17.1810088682572
16-17	22.85545116873851	27.243610565622557	31.566102385193812	18.33483588044512
18-19	23.414717819372942	28.12319898548627	30.312510803712637	18.149572391428148
20-21	25.332013523303914	27.748379015704373	30.985845664287286	15.933761796704427
22-23	21.26218136408483	23.764330709289617	33.76120784272606	21.212280083899497
24-25	19.002453083868723	27.740628835911597	34.89793887007697	18.358979210142707
26-27	18.44438315217288	33.034628487153505	29.813668961707652	18.707319398965957
28-29	16.87985359758407	29.05428431078677	35.00494073961789	19.060921352011267
30-31	21.94125287734923	27.66485563200627	31.965236644521667	18.42865484612284
32-33	24.20848439535491	27.559411396639383	30.270074770239464	17.962029437766247
34-35	19.50378334144443	28.27328273681643	31.508887822605985	20.714046099133157
36-37	22.092894262853427	28.24893045619306	31.232445747791676	18.425729533161842
38-39	19.720788375642094	30.476195360757576	29.638055206354235	20.164961057246096
40-41	20.640818297414672	26.839005591146865	30.45492035740486	22.065255754033608
42-43	20.837137189959563	26.28667231214077	30.666910569383678	22.209279928515986
44-45	19.367547337832235	29.714626222885293	29.818094922226567	21.099731517055904
46-47	20.54629649680476	28.794710274344972	30.27592539616146	20.383067832688813
48-49	21.140154023426057	26.8398223993113	31.80234990010246	20.21767367716018
50-51	19.190091015224166	29.310629105648246	30.764908553577108	20.734371325550484
52-53	21.440017597467058	26.284905727040947	31.166588215091807	21.108488460400192
54-55	21.61380778110453	26.896467095737897	31.126184704260357	20.36354041889722
56-57	21.727591057964126	26.45786010697528	30.292299550527563	21.522249284533036
58-59	22.90531445784643	26.779701519301174	30.174660179310287	20.140323843542106
60-61	22.803669330221105	28.310494997144968	28.88894714187426	19.996888530759666
62-63	22.49168660248454	25.780327232346213	31.60257381951275	20.1254123456565
64-65	19.842378818910593	28.94222962794958	31.466679735597296	19.74871181754253
66-67	19.052069430973443	28.344288060506113	28.753432968732962	23.850209539787485
68-69	18.319962373636926	30.068703064398296	28.644189625624527	22.96714493634025
70-71	19.1808971744896	28.75552247799082	30.839238096540644	21.224342250978935
72-73	21.573879158740777	28.595580042071315	29.563231779384292	20.26730901980361
74-75	20.32581527902357	30.238200256287804	27.717473274226816	21.718511190461808
76-77	23.440000881392997	27.75996629431612	28.562128898406918	20.23790392588397
78-79	19.097050866633463	28.630816766374252	31.223479853521603	21.048652513470685
80-81	17.475040811914912	28.012986869903735	32.037362704995104	22.474609613186246
82-83	18.638213629147057	27.39696154961046	32.132473367304954	21.83235145393753
84-85	17.701391651156808	27.111135678674508	31.382263561579926	23.805209108588755
86	17.901927553294833	27.404521774016153	31.974582449565897	22.718968223123117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1974.0
1	2543.5
2	7934.0
3	7968.0
4	3512.0
5	4442.5
6	5295.0
7	5548.0
8	5935.0
9	6173.5
10	6184.5
11	6369.0
12	6380.0
13	6369.5
14	6373.0
15	6258.5
16	6268.0
17	6801.5
18	7440.5
19	7931.5
20	8151.0
21	8109.0
22	8616.0
23	10039.0
24	13024.5
25	17153.0
26	20648.5
27	25703.0
28	32294.5
29	35268.0
30	41439.5
31	48685.0
32	52111.0
33	59995.0
34	65334.5
35	69400.0
36	73658.0
37	77152.0
38	82035.5
39	91284.0
40	104870.0
41	118128.0
42	128591.5
43	131524.0
44	131432.5
45	129458.5
46	132684.5
47	140330.5
48	141641.5
49	127681.5
50	114948.0
51	104460.0
52	89396.0
53	80677.0
54	70298.0
55	61784.5
56	55014.5
57	50522.0
58	46605.5
59	37091.0
60	26927.0
61	20849.5
62	16213.0
63	10891.5
64	8695.0
65	7696.5
66	5915.5
67	4165.5
68	3185.5
69	2658.0
70	1685.5
71	1228.0
72	1127.5
73	962.0
74	711.5
75	351.5
76	170.5
77	198.0
78	179.0
79	126.0
80	108.5
81	70.0
82	39.0
83	24.5
84	16.0
85	16.5
86	21.0
87	13.5
88	6.5
89	4.5
90	2.0
91	1.0
92	1.0
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2632197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.121932283902474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.71717541464317	12.690729716149496
2	9.13234857944148	2.9446221078149692
3	2.9178337503665874	1.4112335441728596
4	1.4574484765388713	0.9398754258414601
5	0.841606866176067	0.678416445307896
6	0.5491205468727176	0.5311730563428868
7	0.4317392125092831	0.4872329243866029
8	0.32181887474539855	0.41506736850616055
9	0.24441203318696686	0.3546354823570083
>10	2.4679260926612723	9.181816056575926
>50	1.0748045831572157	12.778188281848779
>100	1.8345063813027147	55.60072005790704
>500	0.006410207352649437	0.6666513429796217
>1k	0.002611565958486808	0.6274950977095894
>5k	0.0	0.0
>10k+	2.3741508713516433E-4	0.6921430920997015
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18083	0.6869926529055387	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038295006034882645	0.0
2	0.0	0.0	0.0	0.14588573727574342	0.0
3	0.0	0.0	0.0	0.2082290953146744	0.0
4	0.0	0.0	0.0	0.30240897622784313	0.0
5	0.0	0.0	0.0	0.5329768250628657	0.0
6	0.0	0.0	0.0	0.6925773412856256	0.0
7	0.0	0.0	0.0	0.7987624026621107	0.0
8	0.0	0.0	0.0	1.009498909086212	0.0
9	0.0	0.0	0.0	1.0858229836140685	0.0
10	0.0	0.0	0.0	1.2484247949526575	0.0
11	0.0	0.0	0.0	1.567131943391775	0.0
12	0.0	0.0	0.0	1.810312829928763	0.0
13	0.0	0.0	0.0	1.9011874871067782	0.0
14	0.0	0.0	0.0	1.9386846805159341	0.0
15	0.0	0.0	0.0	1.9992804489937492	0.0
16	0.0	0.0	0.0	2.123625245374871	0.0
17	0.0	0.0	0.0	2.2935973257320788	0.0
18	0.0	0.0	0.0	2.476372399178329	0.0
19	0.0	0.0	0.0	2.6197507253446455	0.0
20	0.0	0.0	0.0	2.777223741232134	0.0
21	0.0	0.0	0.0	2.9591630109752423	0.0
22	0.0	0.0	0.0	3.159034069258494	0.0
23	0.0	0.0	0.0	3.3495973135749337	0.0
24	0.0	0.0	0.0	3.492139836038108	0.0
25	0.0	0.0	0.0	3.6266662411665997	0.0
26	0.0	0.0	0.0	3.7500992516897482	0.0
27	0.0	0.0	0.0	3.8967448105138027	0.0
28	0.0	0.0	0.0	4.040693002841353	0.0
29	0.0	0.0	0.0	4.227267184029159	0.0
30	0.0	0.0	0.0	4.444994048697723	0.0
31	0.0	0.0	0.0	4.626363452279597	0.0
32	0.0	0.0	0.0	4.7801133425803615	0.0
33	3.799107741555818E-5	0.0	0.0	4.946096359808935	0.0
34	3.799107741555818E-5	0.0	0.0	5.122033039320385	0.0
35	3.799107741555818E-5	0.0	0.0	5.327146866286983	0.0
36	3.799107741555818E-5	0.0	0.0	5.5008800633083315	0.0
37	3.799107741555818E-5	0.0	0.0	5.68794812850254	0.0
38	3.799107741555818E-5	0.0	0.0	5.89997633155877	0.0
39	3.799107741555818E-5	0.0	0.0	6.245923082504843	0.0
40	3.799107741555818E-5	0.0	0.0	6.460002803741514	0.0
41	3.799107741555818E-5	0.0	0.0	6.673284712352457	0.0
42	3.799107741555818E-5	0.0	0.0	6.89264519334989	0.0
43	3.799107741555818E-5	0.0	0.0	7.101368172670966	0.0
44	3.799107741555818E-5	0.0	0.0	7.297136194593338	0.0
45	3.799107741555818E-5	0.0	0.0	7.5027818966437545	0.0
46	7.598215483111636E-5	0.0	0.0	7.731678138072493	0.0
47	7.598215483111636E-5	0.0	0.0	7.973757283364429	0.0
48	7.598215483111636E-5	0.0	0.0	8.19604307732286	0.0
49	7.598215483111636E-5	0.0	0.0	8.416429317410513	0.0
50	7.598215483111636E-5	0.0	0.0	8.654177479877076	0.0
51	7.598215483111636E-5	0.0	0.0	8.882959748073567	0.0
52	7.598215483111636E-5	0.0	0.0	9.1714259988899	0.0
53	7.598215483111636E-5	0.0	0.0	9.41673438576216	0.0
54	7.598215483111636E-5	0.0	0.0	9.66246067448599	0.0
55	7.598215483111636E-5	0.0	0.0	9.905945489642303	0.0
56	7.598215483111636E-5	0.0	0.0	10.152241644527367	0.0
57	7.598215483111636E-5	0.0	0.0	10.401881014225	0.0
58	7.598215483111636E-5	0.0	0.0	10.654673643348124	0.0
59	7.598215483111636E-5	0.0	0.0	10.903059307491043	0.0
60	7.598215483111636E-5	0.0	0.0	11.197338193151957	0.0
61	7.598215483111636E-5	0.0	0.0	11.487779979993899	0.0
62	7.598215483111636E-5	0.0	0.0	11.81176788819378	0.0
63	7.598215483111636E-5	0.0	0.0	12.120445392195188	0.0
64	7.598215483111636E-5	0.0	0.0	12.408303785772873	0.0
65	7.598215483111636E-5	0.0	0.0	12.691983160834846	0.0
66	7.598215483111636E-5	0.0	0.0	12.957578783047014	0.0
67	7.598215483111636E-5	0.0	0.0	13.245057265850543	0.0
68	7.598215483111636E-5	0.0	0.0	13.577099282462521	0.0
69	7.598215483111636E-5	0.0	0.0	14.051417883995764	0.0
70	7.598215483111636E-5	0.0	0.0	14.362412843719524	0.0
71	7.598215483111636E-5	0.0	0.0	14.65350047887753	0.0
72	1.1397323224667454E-4	0.0	0.0	14.974563074116412	0.0
73	1.1397323224667454E-4	0.0	0.0	15.279479461453683	0.0
74	1.1397323224667454E-4	0.0	0.0	15.610305763588364	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	1200	0.0	68.333336	4
CGAGGGA	1615	0.0	67.36842	4
CTACGGG	465	0.0	67.09677	3
TAGGGAC	2965	0.0	66.913994	5
CGAACGG	120	0.0	66.66667	2
CGTTTTT	8425	0.0	66.65875	1
CGAAACG	380	0.0	66.31579	80
AGGGATT	5730	0.0	65.2007	6
ACGGGAT	1290	0.0	64.18604	5
TATTGCG	325	0.0	64.0	1
TATAGCG	125	0.0	64.0	1
TTAGGGA	4465	0.0	63.964165	4
CGGGATA	650	0.0	63.384617	6
TACGCGG	260	0.0	63.07692	2
ATTGCGG	650	0.0	62.76923	2
TCGATAG	255	0.0	62.7451	1
TTGCGGG	2175	0.0	62.344826	3
ATAGGGA	3795	0.0	62.29249	4
CGTAAGG	560	0.0	62.142857	2
ACGGGAC	895	0.0	61.22905	5
>>END_MODULE