FastQCFastQC Report
Thu 26 May 2016
SRR1547766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547766_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1698558
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95530.5624182394713634No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19650.11568636455157846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18860.11103536058232925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG1650.072.727271
TACGGGA7450.071.946314
TTGTACG1450.071.724141
CGTTTTT54700.070.1279751
TAGGGAC19000.069.894745
GGGCGAT7450.069.261757
TAGCGGG16300.068.711663
TAGGGTA11400.068.0701755
CGGGACT7600.067.894746
ATAGCGG3450.067.2463762
TAAGTCG900.066.6666641
ATATACG900.066.6666641
AGTTGCG1450.066.2068941
CGTTAGG2300.066.086962
GTACGGG6050.065.454553
TCTAGCG559.094947E-1265.4545441
TTATAGG7100.065.352112
AGGGTAA12600.065.079366
TCGTTAG1050.064.761911
GTTAGCG2600.064.615381
Produced by FastQC (version 0.11.5)