##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547766_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1698558
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75556324835537	31.0	31.0	33.0	30.0	34.0
2	32.19415115645153	33.0	31.0	34.0	30.0	34.0
3	32.34636615293679	34.0	31.0	34.0	30.0	34.0
4	35.944803768843926	37.0	35.0	37.0	35.0	37.0
5	35.880273149341974	37.0	35.0	37.0	35.0	37.0
6	35.94214504303062	37.0	35.0	37.0	35.0	37.0
7	36.22712500838947	37.0	35.0	37.0	35.0	37.0
8	36.190547511477384	37.0	35.0	37.0	35.0	37.0
9	38.077345607273934	39.0	39.0	39.0	37.0	39.0
10-11	37.48712672749474	39.0	37.5	39.0	35.0	39.0
12-13	36.45612690293767	39.0	35.0	39.0	33.0	39.0
14-15	37.46455051873413	40.0	35.0	41.0	33.0	41.0
16-17	37.71158977203016	40.0	35.0	41.0	33.0	41.0
18-19	37.633635118730126	39.0	35.5	41.0	33.5	41.0
20-21	37.41737344264959	39.0	35.0	41.0	33.0	41.0
22-23	37.18937269142414	39.0	35.0	41.0	33.0	41.0
24-25	37.11521155003244	39.0	35.0	41.0	33.0	41.0
26-27	37.091674526274645	39.0	35.0	41.0	33.0	41.0
28-29	37.11418597422049	39.0	35.0	41.0	33.0	41.0
30-31	36.965543125404025	38.0	35.0	41.0	32.5	41.0
32-33	36.62318007392153	38.0	35.0	41.0	31.5	41.0
34-35	36.36998324461102	38.0	35.0	41.0	31.0	41.0
36-37	36.164009412690056	38.0	35.0	41.0	30.5	41.0
38-39	36.08343636190227	38.0	35.0	41.0	30.0	41.0
40-41	35.9229673052083	38.0	35.0	40.5	30.0	41.0
42-43	35.90411219399043	38.0	35.0	40.0	30.0	41.0
44-45	35.786621357645714	38.0	35.0	40.0	30.0	41.0
46-47	35.70250530155579	37.5	35.0	40.0	30.0	41.0
48-49	35.58792693567132	37.0	35.0	40.0	29.0	41.0
50-51	35.40999218160346	37.0	35.0	40.0	29.0	41.0
52-53	35.22452515604412	36.0	34.0	40.0	28.5	41.0
54-55	35.02018918400196	36.0	34.0	40.0	28.0	41.0
56-57	34.70163279676055	36.0	34.0	39.5	27.5	41.0
58-59	34.399768509523966	35.0	34.0	39.0	26.0	41.0
60-61	34.089424382329014	35.0	34.0	39.0	26.0	41.0
62-63	33.669916187731005	35.0	33.0	38.5	25.5	40.0
64-65	33.38487823200621	35.0	33.0	38.0	25.0	40.0
66-67	33.12949955197291	35.0	33.0	37.0	24.5	40.0
68-69	32.812286068535784	35.0	33.0	36.5	23.5	39.5
70-71	32.57719018131851	35.0	33.0	36.0	24.0	39.0
72-73	32.33249409204749	35.0	32.5	36.0	23.0	39.0
74-75	32.04261055554181	35.0	32.0	35.0	22.0	37.5
76-77	29.97264002759988	32.5	29.0	34.5	18.5	35.5
78-79	31.055852081589208	34.0	30.5	35.0	20.0	36.0
80-81	31.426446727165043	34.5	32.0	35.0	20.0	36.0
82-83	31.30300643251511	34.5	32.0	35.0	19.5	36.0
84-85	31.134169101084566	34.0	32.0	35.0	19.0	36.0
86	30.759067397168657	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	15.0
11	12.0
12	19.0
13	71.0
14	253.0
15	746.0
16	1750.0
17	3591.0
18	6989.0
19	11070.0
20	13838.0
21	14245.0
22	13122.0
23	12690.0
24	13415.0
25	15182.0
26	17847.0
27	21783.0
28	26674.0
29	33273.0
30	41036.0
31	50717.0
32	62850.0
33	83883.0
34	220445.0
35	195100.0
36	143379.0
37	242810.0
38	307210.0
39	144072.0
40	464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.306555325164048	23.1539929752178	31.10179340358116	14.437658296036993
2	29.051230514353943	25.210443211241536	32.3777580747905	13.360568199614026
3	28.150172087146863	24.317568196081616	33.458969313971025	14.073290402800492
4	26.75069088014657	27.392647174838892	31.302846296682247	14.553815648332291
5	24.238206761264554	32.26195396330299	29.85856238056045	13.641276894872004
6	22.573912695356885	40.643121989358036	26.16442888614931	10.618536429135773
7	88.91966008814536	3.5370002084120764	5.900357832938292	1.642981870504275
8	89.96360442210393	2.6165135367764893	5.668278622219553	1.7516034189000316
9	86.05163909622162	4.859180551974086	6.785049436051051	2.304130915753245
10-11	43.61275858698967	26.969523560573144	18.87518707044446	10.542530781992726
12-13	30.908541244985454	26.6894330367288	27.09972223497814	15.302303483307606
14-15	15.010526576072175	33.18208739413079	35.81767593452799	15.989710095269047
16-17	19.479905896648805	24.21044792111897	34.33630173358814	21.973344448644085
18-19	24.00456740364474	26.207347644295925	31.12551941117112	18.662565540888213
20-21	26.830582176175323	26.702944497626813	29.05517503670761	17.41129828949026
22-23	20.566180254074336	23.77042762154722	30.824587679667104	24.838804444711336
24-25	17.877723339444398	26.32094400073474	36.76765821361413	19.033674446206724
26-27	17.46290088416174	34.852739794578696	28.4886062177447	19.195753103514864
28-29	15.406862762413764	27.46144082215621	37.587824495837054	19.543871919592974
30-31	23.08758370335308	28.45416523898507	30.306972149317247	18.151278908344608
32-33	26.341726334926452	27.609389847152705	28.4060950523915	17.64278876552935
34-35	19.238789608597408	28.410687182892786	30.025674719379612	22.324848489130193
36-37	24.12584674765301	27.869581138824817	30.253573913872827	17.750998199649352
38-39	19.09092889380286	30.398314334865223	29.54108720455822	20.9696695667737
40-41	20.36957230780462	25.397130978159122	29.645381553058535	24.587915160977726
42-43	21.717244862995553	24.51093810161325	28.745471158476775	25.02634587691442
44-45	18.825821667555655	32.01762907124749	28.525107767883114	20.63144149331374
46-47	22.155057407518612	29.617063414967284	27.229008370629675	20.99887080688443
48-49	21.724574609757216	25.77751245468215	32.362186042513706	20.135726893046925
50-51	19.665533941143014	31.15698727979851	29.08885066038369	20.08862811867478
52-53	23.27238751929578	25.71275163992045	30.07109559991475	20.94376524086902
54-55	23.06918574461396	26.67739341252992	30.257400689290563	19.996020153565556
56-57	23.410416364940144	24.02867608877648	30.1107468805893	22.450160665694078
58-59	24.23087701450289	25.453767254341624	29.23217811814492	21.083177613010566
60-61	24.427249466900747	28.16527313167993	26.95262687526714	20.454850526152182
62-63	23.756209679033628	24.893792263790814	31.76288357536216	19.587114481813398
64-65	20.175966908401126	29.70340135573822	30.543143066059564	19.57748866980109
66-67	18.020726993131824	28.824567662688	26.003351077796577	27.151354266383603
68-69	18.088814158833554	31.869267931975244	24.727033165779446	25.314884743411763
70-71	19.142060500730622	29.671020948357373	28.9545897166891	22.232328834222912
72-73	23.78626458442985	28.12912482234931	28.03095331451737	20.053657278703465
74-75	23.211011928942078	31.01445461385481	23.757416585126915	22.017116872076194
76-77	27.934371390320496	27.386877574978307	25.67000950217773	19.00874153252347
78-79	19.853281430483975	28.170306813190955	31.23811491865453	20.738296837670543
80-81	16.54441591043697	27.94543960229795	32.311437113127724	23.198707374137356
82-83	19.66709408804409	25.157251032934997	33.109702465267596	22.065952413753312
84-85	16.442505937389242	25.983893396634084	31.310382100581784	26.26321856539488
86	19.114095603447158	24.99802773882317	32.198959352580246	23.688917305149428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1543.0
1	1521.0
2	3853.0
3	4005.0
4	1916.0
5	2351.0
6	2934.0
7	3195.0
8	3442.0
9	3567.5
10	3363.5
11	3373.5
12	3428.5
13	3310.5
14	3230.0
15	3289.0
16	3402.5
17	3657.0
18	3901.5
19	3913.0
20	4172.0
21	4464.0
22	5183.0
23	5858.5
24	6692.0
25	7727.0
26	8793.0
27	12215.0
28	15886.0
29	17043.0
30	19935.0
31	24936.5
32	27536.0
33	29422.5
34	31970.5
35	33926.5
36	34731.0
37	38399.0
38	44378.0
39	50662.0
40	61653.0
41	75021.0
42	90773.5
43	100176.0
44	97713.5
45	98728.0
46	104821.5
47	104247.0
48	104361.0
49	101013.0
50	94360.0
51	80218.5
52	58979.5
53	49070.0
54	42661.5
55	38727.0
56	38628.5
57	38868.0
58	34763.0
59	25969.5
60	18336.5
61	13951.5
62	9986.0
63	7040.0
64	6619.0
65	5590.5
66	3955.5
67	2675.0
68	1603.5
69	1296.5
70	1169.0
71	951.0
72	754.5
73	477.5
74	261.5
75	101.0
76	56.5
77	84.0
78	93.0
79	55.0
80	36.5
81	17.0
82	12.0
83	6.5
84	3.5
85	2.0
86	2.0
87	2.5
88	1.5
89	0.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1698558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.455378617091622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87123515675891	12.344401799624682
2	9.204470419619208	2.845171506100701
3	2.939635119422854	1.3629952130033862
4	1.3068706689230847	0.8079272396711227
5	0.7511691563176082	0.5804801858184957
6	0.4855927352767188	0.45030117464465
7	0.37523660366194234	0.40595966464108074
8	0.3031327598495723	0.37480252597752395
9	0.222067363729703	0.30889216664477637
>10	1.8793822990839426	6.489855880522705
>50	0.7480814324849072	8.584519523550856
>100	1.8939643629477267	62.787770320618854
>500	0.01647925285446221	1.5785048620767814
>1k	0.0022994306308551926	0.5125846557753272
>5k	3.8323843847586536E-4	0.5658332813290616
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9553	0.5624182394713634	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1965	0.11568636455157846	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1886	0.11103536058232925	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0555765537591298	0.0
2	0.0	0.0	0.0	0.1877474893409586	0.0
3	0.0	0.0	0.0	0.26027960187406024	0.0
4	0.0	0.0	0.0	0.390802080352864	0.0
5	0.0	0.0	0.0	0.6459008170459885	0.0
6	0.0	0.0	0.0	0.8434801755371321	0.0
7	0.0	0.0	0.0	0.9747091356315181	0.0
8	0.0	0.0	0.0	1.2593034797751976	0.0
9	0.0	0.0	0.0	1.3380761799126082	0.0
10	5.8873467965179875E-5	0.0	0.0	1.513754608320705	0.0
11	5.8873467965179875E-5	0.0	0.0	1.8108890011409677	0.0
12	5.8873467965179875E-5	0.0	0.0	2.0460885056618614	0.0
13	5.8873467965179875E-5	0.0	0.0	2.1256854343507845	0.0
14	5.8873467965179875E-5	0.0	0.0	2.1548278009935484	0.0
15	5.8873467965179875E-5	0.0	0.0	2.20928575886134	0.0
16	5.8873467965179875E-5	0.0	0.0	2.3279746702791426	0.0
17	5.8873467965179875E-5	0.0	0.0	2.484872462406347	0.0
18	5.8873467965179875E-5	0.0	0.0	2.6666148580148574	0.0
19	5.8873467965179875E-5	0.0	0.0	2.7783567002127687	0.0
20	5.8873467965179875E-5	0.0	0.0	2.9000481584967956	0.0
21	5.8873467965179875E-5	0.0	0.0	3.0645995014594734	0.0
22	5.8873467965179875E-5	0.0	0.0	3.2394537013160574	0.0
23	5.8873467965179875E-5	0.0	0.0	3.423551035643175	0.0
24	5.8873467965179875E-5	0.0	0.0	3.551188714191685	0.0
25	2.354938718607195E-4	0.0	0.0	3.6606933646069195	0.0
26	2.354938718607195E-4	0.0	0.0	3.7779104393255927	0.0
27	2.354938718607195E-4	0.0	0.0	3.895892869127813	0.0
28	2.354938718607195E-4	0.0	0.0	4.02747507002999	0.0
29	2.354938718607195E-4	0.0	0.0	4.16818265846677	0.0
30	2.354938718607195E-4	0.0	0.0	4.343095731791319	0.0
31	2.354938718607195E-4	0.0	0.0	4.510119760408535	0.0
32	2.354938718607195E-4	0.0	0.0	4.639818010335826	0.0
33	2.354938718607195E-4	0.0	0.0	4.781055459984293	0.0
34	2.354938718607195E-4	0.0	0.0	4.928710117640963	0.0
35	2.354938718607195E-4	0.0	0.0	5.112984072371977	0.0
36	2.354938718607195E-4	0.0	0.0	5.258872525989692	0.0
37	2.943673398258994E-4	0.0	0.0	5.42524894645929	0.0
38	2.943673398258994E-4	0.0	0.0	5.601928223822796	0.0
39	2.943673398258994E-4	0.0	0.0	5.825470781686583	0.0
40	2.943673398258994E-4	0.0	0.0	6.01910561782406	0.0
41	2.943673398258994E-4	0.0	0.0	6.202437597067631	0.0
42	2.943673398258994E-4	0.0	0.0	6.389949592536728	0.0
43	2.943673398258994E-4	0.0	0.0	6.573104951376402	0.0
44	2.943673398258994E-4	0.0	0.0	6.74071771467327	0.0
45	2.943673398258994E-4	0.0	0.0	6.925874771423761	0.0
46	2.943673398258994E-4	0.0	0.0	7.128929362435666	0.0
47	2.943673398258994E-4	0.0	0.0	7.326508720926809	0.0
48	2.943673398258994E-4	0.0	0.0	7.521262152955624	0.0
49	2.943673398258994E-4	0.0	0.0	7.745040204691273	0.0
50	2.943673398258994E-4	0.0	0.0	7.96163569333517	0.0
51	2.943673398258994E-4	0.0	0.0	8.164101549667423	0.0
52	2.943673398258994E-4	0.0	0.0	8.404658539773148	0.0
53	2.943673398258994E-4	0.0	0.0	8.625728411982399	0.0
54	2.943673398258994E-4	0.0	0.0	8.847092651531476	0.0
55	2.943673398258994E-4	0.0	0.0	9.100601804589541	0.0
56	2.943673398258994E-4	0.0	0.0	9.34427908849742	0.0
57	2.943673398258994E-4	0.0	0.0	9.595080062029085	0.0
58	2.943673398258994E-4	0.0	0.0	9.808143142595071	0.0
59	2.943673398258994E-4	0.0	0.0	10.04022235331381	0.0
60	2.943673398258994E-4	0.0	0.0	10.312571016120733	0.0
61	3.5324080779107925E-4	0.0	0.0	10.578090356643694	0.0
62	3.5324080779107925E-4	0.0	0.0	10.882466186023674	0.0
63	3.5324080779107925E-4	0.0	0.0	11.182720872646092	0.0
64	3.5324080779107925E-4	0.0	0.0	11.478089061427399	0.0
65	3.5324080779107925E-4	0.0	0.0	11.746375454944724	0.0
66	3.5324080779107925E-4	0.0	0.0	12.006007448671166	0.0
67	3.5324080779107925E-4	0.0	0.0	12.277943997202334	0.0
68	3.5324080779107925E-4	0.0	0.0	12.564304545384967	0.0
69	3.5324080779107925E-4	0.0	0.0	12.935855001713218	0.0
70	3.5324080779107925E-4	0.0	0.0	13.206437460481185	0.0
71	3.5324080779107925E-4	0.0	0.0	13.491385045432656	0.0
72	3.5324080779107925E-4	0.0	0.0	13.762673985816203	0.0
73	3.5324080779107925E-4	0.0	0.0	14.03949703218848	0.0
74	3.5324080779107925E-4	0.0	0.0	14.35865010202772	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTAG	165	0.0	72.72727	1
TACGGGA	745	0.0	71.94631	4
TTGTACG	145	0.0	71.72414	1
CGTTTTT	5470	0.0	70.127975	1
TAGGGAC	1900	0.0	69.89474	5
GGGCGAT	745	0.0	69.26175	7
TAGCGGG	1630	0.0	68.71166	3
TAGGGTA	1140	0.0	68.070175	5
CGGGACT	760	0.0	67.89474	6
ATAGCGG	345	0.0	67.246376	2
TAAGTCG	90	0.0	66.666664	1
ATATACG	90	0.0	66.666664	1
AGTTGCG	145	0.0	66.206894	1
CGTTAGG	230	0.0	66.08696	2
GTACGGG	605	0.0	65.45455	3
TCTAGCG	55	9.094947E-12	65.454544	1
TTATAGG	710	0.0	65.35211	2
AGGGTAA	1260	0.0	65.07936	6
TCGTTAG	105	0.0	64.76191	1
GTTAGCG	260	0.0	64.61538	1
>>END_MODULE