FastQCFastQC Report
Thu 26 May 2016
SRR1547765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547765_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1794417
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105880.5900523679835846No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20350.11340730722011663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG600.073.333331
CGTTTTT51250.068.3707351
CGTTAGG3650.067.9452062
TACGGGA8600.067.9069754
CGAAACG3300.066.66666480
CGCGAAC303.7964528E-666.66666480
CGTAAGG2050.066.341462
CTATGCG1450.066.20691
CGGGATC6650.066.165416
GTACGGG6900.066.086953
GCGGGAT13550.065.830255
TAGCGCG559.094947E-1265.4545441
ACGGGAT9400.064.680855
TCGTTAG2850.064.561411
TACGCGG1750.064.02
TACGGGG10850.063.7788054
TTAGGGA26950.063.5250434
TAGGGAT35150.062.7027055
AGGGATC26550.062.67426
TTACGGG6750.062.222223
Produced by FastQC (version 0.11.5)