FastQCFastQC Report
Thu 26 May 2016
SRR1547764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547764_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1712412
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112220.6553329455761815No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36220.2115145187022749No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22560.1317439961878333No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTA150.00130990180.000011
TATAGCG550.072.727271
TACGTAG1100.072.727271
CGTTTTT58750.070.944681
CGAACGG750.069.3333362
ACGTTAG351.1818338E-768.571431
GGCGATA1650.067.8787848
CGTATGG1900.067.368422
GTACGGG5700.065.964913
GGGCGAT8100.065.679017
TAGGGAC16400.064.878055
CTATGCG1050.064.761911
AACGTAG1000.064.01
CTAGACG503.0013325E-1064.01
TGCGTCG1000.064.01
TCGTTAG950.063.1578941
TAGAACG1400.062.8571431
TAGCGGG10150.062.6600953
TATTGCG900.062.222221
TTAGGGA20100.061.4925424
Produced by FastQC (version 0.11.5)