##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547764_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1712412
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74050286963651	31.0	31.0	33.0	30.0	34.0
2	32.17216300750053	33.0	31.0	34.0	30.0	34.0
3	32.315615050583624	34.0	31.0	34.0	30.0	34.0
4	35.90535688841237	37.0	35.0	37.0	35.0	37.0
5	35.84935284265703	37.0	35.0	37.0	35.0	37.0
6	35.90257075984051	37.0	35.0	37.0	35.0	37.0
7	36.17526915251703	37.0	35.0	37.0	35.0	37.0
8	36.11808256424272	37.0	35.0	37.0	35.0	37.0
9	37.954642924716715	39.0	39.0	39.0	35.0	39.0
10-11	37.45786177625478	39.0	37.0	39.0	34.5	39.0
12-13	36.478893805929886	39.0	35.0	39.0	33.0	39.0
14-15	37.45310474348463	40.0	35.0	41.0	33.0	41.0
16-17	37.66075249414276	39.5	35.0	41.0	33.0	41.0
18-19	37.53434074276518	39.0	35.0	41.0	33.0	41.0
20-21	37.29781413585049	39.0	35.0	41.0	33.0	41.0
22-23	37.10206743470613	39.0	35.0	41.0	32.5	41.0
24-25	37.010824497842805	38.0	35.0	41.0	33.0	41.0
26-27	36.98395362798205	38.0	35.0	41.0	33.0	41.0
28-29	36.98924966655221	38.5	35.0	41.0	33.0	41.0
30-31	36.811004302702855	38.0	35.0	41.0	32.0	41.0
32-33	36.451750805296854	38.0	35.0	41.0	31.0	41.0
34-35	36.17031532131286	38.0	35.0	41.0	30.5	41.0
36-37	35.94577093596635	38.0	35.0	41.0	30.0	41.0
38-39	35.8586534665723	38.0	35.0	41.0	30.0	41.0
40-41	35.694346045227434	38.0	35.0	40.5	29.0	41.0
42-43	35.6461829863374	38.0	35.0	40.0	29.0	41.0
44-45	35.504879374823346	37.5	35.0	40.0	29.0	41.0
46-47	35.439125338995524	37.0	35.0	40.0	29.0	41.0
48-49	35.33398124983941	37.0	35.0	40.0	28.5	41.0
50-51	35.1446401333324	36.5	34.0	40.0	28.0	41.0
52-53	34.919755584520544	36.0	34.0	40.0	27.0	41.0
54-55	34.69697099763374	36.0	34.0	40.0	27.0	41.0
56-57	34.36409637400345	35.5	34.0	39.5	25.5	41.0
58-59	34.05735652401408	35.0	34.0	39.0	25.5	41.0
60-61	33.76302723877198	35.0	33.0	39.0	25.0	40.5
62-63	33.36860492685172	35.0	33.0	38.0	24.0	40.0
64-65	33.06911917225527	35.0	33.0	37.5	23.0	40.0
66-67	32.82598259063823	35.0	33.0	37.0	22.5	40.0
68-69	32.47486556973438	35.0	32.5	36.5	21.0	39.0
70-71	32.24749038198751	35.0	32.0	36.0	20.5	39.0
72-73	32.016267405273965	35.0	32.0	35.5	20.0	38.5
74-75	31.74118115266653	35.0	32.0	35.0	20.0	37.5
76-77	29.705593922490614	32.5	28.5	34.5	17.0	35.5
78-79	30.791296428663195	34.0	30.5	35.0	18.0	36.0
80-81	31.127370048796667	34.5	31.0	35.0	19.0	36.0
82-83	30.986889837258794	34.5	31.0	35.0	18.0	36.0
84-85	30.798390515833805	34.0	31.0	35.0	17.5	36.0
86	30.421647360565096	34.0	31.0	35.0	16.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	9.0
11	13.0
12	31.0
13	90.0
14	310.0
15	957.0
16	2331.0
17	4618.0
18	8751.0
19	13168.0
20	16006.0
21	16217.0
22	14943.0
23	14366.0
24	14784.0
25	16633.0
26	19536.0
27	23503.0
28	28655.0
29	35382.0
30	43558.0
31	52358.0
32	63484.0
33	85402.0
34	218428.0
35	196682.0
36	143842.0
37	238838.0
38	306236.0
39	132706.0
40	573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.74509171858174	22.44383944985202	29.35152288117579	14.459545950390442
2	31.593331511341898	24.662464406930106	29.98723438051123	13.756969701216763
3	29.868045774031017	23.641565230797262	31.911888026946787	14.578500968224937
4	26.4118681719119	27.147964391746847	31.865754269416474	14.574413166924781
5	24.670756803853276	30.554737995295522	30.078567540988963	14.69593765986223
6	23.50240479510772	39.68349906447747	26.848328556445527	9.96576758396928
7	85.45303349894769	4.606543285143996	7.797422582883091	2.14300063302523
8	85.15678469900935	4.420022751534093	7.9747747621483605	2.4484177873081947
9	80.38205758894472	6.612077000161176	9.703914712113674	3.3019506987804337
10-11	42.859574681793866	26.045922359805935	19.68971252245371	11.40479043594649
12-13	31.180726367252742	26.982496034832735	26.80365472795098	15.033122869963536
14-15	15.575836889720465	32.344552595987416	36.51127765981551	15.56833285447661
16-17	20.028065675783633	23.45559947022095	35.41016998245749	21.106164871537924
18-19	24.336900231953525	25.89233782524299	31.58407556125512	18.186686381548366
20-21	26.672377909054596	26.406992008932427	29.934063765028508	16.986566316984465
22-23	20.946711422251187	24.49098114238863	31.133307872170953	23.428999563189233
24-25	19.101828298330076	25.69080922114538	36.801920332256486	18.405442148268055
26-27	17.82733944868408	33.860513708149675	29.86757859673957	18.444568246426677
28-29	16.430099765710587	28.308053202149953	36.61402746535297	18.6478195667865
30-31	23.394778826590798	28.975620353045876	30.642187744538113	16.987413075825213
32-33	26.26029833941832	28.58173149919529	28.100509690424968	17.057460470961427
34-35	19.90099345251026	28.93719502082443	29.55004403145972	21.611767495205594
36-37	23.40838536520417	28.758850089814835	30.51292562771109	17.31983891726991
38-39	19.647257786093533	31.200756593623495	28.60056458375671	20.551421036526257
40-41	20.32965781599288	26.590621883051508	29.589695704071218	23.490024596884396
42-43	21.739190101447548	25.664559697082247	28.5457004505925	24.05054975087771
44-45	18.771884336246185	32.49127546408224	28.020885160814103	20.715955038857473
46-47	21.81154418446028	30.947283714433212	27.352996825530308	19.888175275576206
48-49	21.700385187676797	26.968568311831497	31.02109772648171	20.309948774009992
50-51	19.289808760975745	31.61555163126631	28.258882792225236	20.83575681553271
52-53	22.564108403818707	26.63716442071184	30.708088941212747	20.09063823425671
54-55	23.023810858601784	26.620258442477628	31.030820853859936	19.325109845060652
56-57	24.075339345905075	24.03084070889482	29.831927129686083	22.06189281551402
58-59	24.074988962936487	27.15354132066349	28.49880752996358	20.272662186436442
60-61	24.020329219837283	29.18430260941876	27.617331576746718	19.178036593997238
62-63	23.761629794698937	25.55150279255226	31.047201257641266	19.639666155107534
64-65	19.861020595510894	30.427811764925732	30.677722417268743	19.033445222294635
66-67	18.31013798081303	29.287198407859787	26.082332989958022	26.32033062136916
68-69	17.79504581841286	32.79829854030456	24.580503990862013	24.826151650420577
70-71	19.48176023059871	30.23329082019981	28.50996138779686	21.774987561404615
72-73	23.451248881694358	30.061369576947605	26.836941109966528	19.650440431391512
74-75	21.61305223275707	32.496706400095306	24.46800185936562	21.422239507782006
76-77	27.39431281724258	27.752229019651814	26.260064750772592	18.593393412333015
78-79	19.236054173878717	29.799399910769136	30.24289715325518	20.721648762096972
80-81	17.10178391648739	28.78142059270783	31.77007052041214	22.34672497039264
82-83	19.274888286230183	26.69053942625957	32.72182745741095	21.312744830099298
84-85	17.495497578853687	26.261904261357667	30.676934055589427	25.56566410419922
86	19.258741471094574	26.93335482348874	31.307302214653948	22.500601490762737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1693.0
1	2061.5
2	5198.0
3	5074.5
4	2281.0
5	2794.0
6	3625.0
7	4041.0
8	4023.0
9	3943.5
10	3814.0
11	3857.0
12	4032.0
13	4253.5
14	4411.0
15	4536.0
16	4725.5
17	4887.0
18	5397.5
19	5934.0
20	5675.5
21	5294.0
22	6200.5
23	7200.0
24	8115.0
25	9340.0
26	11049.5
27	13687.5
28	16241.0
29	17463.0
30	19829.5
31	22371.5
32	24086.5
33	28889.0
34	34308.0
35	38678.5
36	40893.0
37	44539.5
38	48629.5
39	55238.0
40	64849.0
41	74876.0
42	86748.5
43	92040.0
44	96120.5
45	99148.5
46	99405.0
47	103508.5
48	102904.0
49	91847.0
50	84189.0
51	75231.5
52	57812.0
53	45434.0
54	42250.5
55	40248.5
56	35964.5
57	34415.0
58	31507.5
59	24206.0
60	17403.5
61	13434.0
62	11234.5
63	8959.5
64	7323.0
65	6545.5
66	5064.5
67	3743.0
68	2580.0
69	1831.5
70	1458.0
71	1288.0
72	1071.5
73	638.5
74	325.0
75	223.5
76	221.5
77	134.5
78	40.0
79	35.0
80	26.0
81	13.5
82	9.5
83	7.0
84	6.0
85	7.0
86	7.0
87	5.0
88	2.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1712412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.411951326569362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.39717279503617	13.1947448670592
2	8.87614976116162	2.9134987569504958
3	2.757843522421607	1.3578478096883406
4	1.3071603108945093	0.8581220559369586
5	0.7338051802425714	0.602158745066277
6	0.5156744456364928	0.5077934341285056
7	0.3404041674290982	0.391068763904541
8	0.27719288597836744	0.3639420922198606
9	0.22895434986120217	0.3381828881335545
>10	1.9951118162801713	7.324619563891025
>50	0.7538265921313708	9.139120019780485
>100	1.799156665638605	60.13712335404281
>500	0.013966383351892039	1.4084417253771793
>1k	0.0032230115427443165	0.8037827172533091
>5k	0.0	0.0
>10k+	3.5811239363825736E-4	0.6595532065674639
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11222	0.6553329455761815	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3622	0.2115145187022749	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2256	0.1317439961878333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06534642364103965	0.0
2	0.0	0.0	0.0	0.21910614968827596	0.0
3	0.0	0.0	0.0	0.3047747855072261	0.0
4	0.0	0.0	0.0	0.436343590210767	0.0
5	0.0	0.0	0.0	0.7004155542007414	0.0
6	0.0	0.0	0.0	0.9088350233471851	0.0
7	0.0	0.0	0.0	1.040929402503603	0.0
8	0.0	0.0	0.0	1.3158048413582712	0.0
9	0.0	0.0	0.0	1.3964513212941745	0.0
10	0.0	0.0	0.0	1.5561091606459194	0.0
11	5.839716143077717E-5	0.0	0.0	1.8391602021008964	0.0
12	5.839716143077717E-5	0.0	0.0	2.0605438410849723	0.0
13	5.839716143077717E-5	0.0	0.0	2.1407815408908606	0.0
14	5.839716143077717E-5	0.0	0.0	2.180199624856635	0.0
15	5.839716143077717E-5	0.0	0.0	2.246480403080567	0.0
16	5.839716143077717E-5	0.0	0.0	2.382837775021432	0.0
17	5.839716143077717E-5	0.0	0.0	2.5416780541131456	0.0
18	5.839716143077717E-5	0.0	0.0	2.7252203324900783	0.0
19	5.839716143077717E-5	0.0	0.0	2.8427738184502327	0.0
20	5.839716143077717E-5	0.0	0.0	2.9707803963064965	0.0
21	5.839716143077717E-5	0.0	0.0	3.1348180227655496	0.0
22	5.839716143077717E-5	0.0	0.0	3.305804911434865	0.0
23	5.839716143077717E-5	0.0	0.0	3.483215487861566	0.0
24	5.839716143077717E-5	0.0	0.0	3.609119767906322	0.0
25	5.839716143077717E-5	0.0	0.0	3.721475906499137	0.0
26	5.839716143077717E-5	0.0	0.0	3.8416572647236764	0.0
27	5.839716143077717E-5	0.0	0.0	3.964875275342616	0.0
28	5.839716143077717E-5	0.0	0.0	4.103276547933558	0.0
29	5.839716143077717E-5	0.0	0.0	4.246057607631808	0.0
30	5.839716143077717E-5	0.0	0.0	4.427147205228648	0.0
31	5.839716143077717E-5	0.0	0.0	4.596849356346486	0.0
32	5.839716143077717E-5	0.0	0.0	4.747981210129338	0.0
33	5.839716143077717E-5	0.0	0.0	4.899463446880774	0.0
34	5.839716143077717E-5	0.0	0.0	5.064318633599858	0.0
35	5.839716143077717E-5	0.0	0.0	5.268591904284716	0.0
36	5.839716143077717E-5	0.0	0.0	5.432863119389492	0.0
37	5.839716143077717E-5	0.0	0.0	5.605835511547455	0.0
38	5.839716143077717E-5	0.0	0.0	5.784238839718479	0.0
39	5.839716143077717E-5	0.0	0.0	6.027404619916235	0.0
40	5.839716143077717E-5	0.0	0.0	6.231911479246817	0.0
41	5.839716143077717E-5	0.0	0.0	6.434724820895906	0.0
42	5.839716143077717E-5	0.0	0.0	6.641100389392272	0.0
43	5.839716143077717E-5	0.0	0.0	6.825635419513528	0.0
44	5.839716143077717E-5	0.0	0.0	7.017178109006477	0.0
45	5.839716143077717E-5	0.0	0.0	7.216954798261166	0.0
46	5.839716143077717E-5	0.0	0.0	7.431622763680703	0.0
47	5.839716143077717E-5	0.0	0.0	7.6579701613863955	0.0
48	5.839716143077717E-5	0.0	0.0	7.8619514462641	0.0
49	5.839716143077717E-5	0.0	0.0	8.085437383059684	0.0
50	5.839716143077717E-5	0.0	0.0	8.325741702347333	0.0
51	5.839716143077717E-5	0.0	0.0	8.558279199164687	0.0
52	5.839716143077717E-5	0.0	0.0	8.825154226903338	0.0
53	5.839716143077717E-5	0.0	0.0	9.049282532474662	0.0
54	5.839716143077717E-5	0.0	0.0	9.29490099345251	0.0
55	5.839716143077717E-5	0.0	0.0	9.55038857471216	0.0
56	5.839716143077717E-5	0.0	0.0	9.800386822797318	0.0
57	5.839716143077717E-5	0.0	0.0	10.055932801218399	0.0
58	5.839716143077717E-5	0.0	0.0	10.286543191708537	0.0
59	5.839716143077717E-5	0.0	0.0	10.541271609869588	0.0
60	5.839716143077717E-5	0.0	0.0	10.80592754547387	0.0
61	5.839716143077717E-5	0.0	0.0	11.091840047838955	0.0
62	5.839716143077717E-5	0.0	0.0	11.378219727495486	0.0
63	5.839716143077717E-5	0.0	0.0	11.681009009514066	0.0
64	5.839716143077717E-5	0.0	0.0	11.977783383905274	0.0
65	5.839716143077717E-5	0.0	0.0	12.250439730625574	0.0
66	5.839716143077717E-5	0.0	0.0	12.507679226728147	0.0
67	5.839716143077717E-5	0.0	0.0	12.771751190718122	0.0
68	5.839716143077717E-5	0.0	0.0	13.075474827319594	0.0
69	5.839716143077717E-5	0.0	0.0	13.46136327005417	0.0
70	5.839716143077717E-5	0.0	0.0	13.742078425051915	0.0
71	5.839716143077717E-5	0.0	0.0	14.028399707547015	0.0
72	5.839716143077717E-5	0.0	0.0	14.319976734570886	0.0
73	1.1679432286155434E-4	0.0	0.0	14.618211037997865	0.0
74	1.1679432286155434E-4	0.0	0.0	14.931570206235415	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTTA	15	0.001309901	80.00001	1
TATAGCG	55	0.0	72.72727	1
TACGTAG	110	0.0	72.72727	1
CGTTTTT	5875	0.0	70.94468	1
CGAACGG	75	0.0	69.333336	2
ACGTTAG	35	1.1818338E-7	68.57143	1
GGCGATA	165	0.0	67.878784	8
CGTATGG	190	0.0	67.36842	2
GTACGGG	570	0.0	65.96491	3
GGGCGAT	810	0.0	65.67901	7
TAGGGAC	1640	0.0	64.87805	5
CTATGCG	105	0.0	64.76191	1
AACGTAG	100	0.0	64.0	1
CTAGACG	50	3.0013325E-10	64.0	1
TGCGTCG	100	0.0	64.0	1
TCGTTAG	95	0.0	63.157894	1
TAGAACG	140	0.0	62.857143	1
TAGCGGG	1015	0.0	62.660095	3
TATTGCG	90	0.0	62.22222	1
TTAGGGA	2010	0.0	61.492542	4
>>END_MODULE