FastQCFastQC Report
Thu 26 May 2016
SRR1547763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547763_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2763790
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149170.539729863701656No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41250.14925157121199512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG1000.072.01
CGTTTTT83650.070.1016161
CGTAAGG3900.069.743592
GCGTAAG3150.068.571431
AGGGCGA10350.066.473436
AGTACGG3450.066.086962
ATAACGG2000.066.02
GCGATAA1900.065.263159
TACGGGA11900.064.873954
CTATACG251.2358325E-464.01
TAATGCG503.0013325E-1064.01
CGAGGGA11800.063.3898284
GGGCGAT16700.062.9940157
GGCGATA3950.062.7848138
AGGGATT50350.062.2840086
GCGAACG1350.062.222221
TGCGCGG4500.062.222222
AGCGGGA14400.061.9444474
GAGGGAT40900.061.6136975
GATCGTT2800.061.4285669
Produced by FastQC (version 0.11.5)