##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547763_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2763790
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80713983334479	31.0	31.0	33.0	30.0	34.0
2	32.249169075798086	33.0	31.0	34.0	31.0	34.0
3	32.40531552686709	34.0	31.0	34.0	31.0	34.0
4	35.98140307331599	37.0	35.0	37.0	35.0	37.0
5	35.942257913951494	37.0	35.0	37.0	35.0	37.0
6	35.99983211459626	37.0	35.0	37.0	35.0	37.0
7	36.22718549528003	37.0	35.0	37.0	35.0	37.0
8	36.17081254364478	37.0	35.0	37.0	35.0	37.0
9	38.02617456463769	39.0	39.0	39.0	35.0	39.0
10-11	37.54527152931301	39.0	37.5	39.0	35.0	39.0
12-13	36.71660545844655	39.0	35.0	39.0	33.0	39.0
14-15	37.75999931253821	40.0	35.0	41.0	33.0	41.0
16-17	37.9065563953846	40.0	35.0	41.0	33.5	41.0
18-19	37.781250384435864	39.5	36.0	41.0	33.5	41.0
20-21	37.60567264517203	39.0	35.0	41.0	33.5	41.0
22-23	37.40118062515604	39.0	35.0	41.0	33.0	41.0
24-25	37.333297211437916	39.0	35.0	41.0	33.0	41.0
26-27	37.30121264640222	39.0	35.0	41.0	33.0	41.0
28-29	37.28841916353993	39.0	35.0	41.0	33.0	41.0
30-31	37.133271341165575	39.0	35.0	41.0	33.0	41.0
32-33	36.83803888862757	39.0	35.0	41.0	32.0	41.0
34-35	36.562629034767475	39.0	35.0	41.0	31.0	41.0
36-37	36.365157446839305	39.0	35.0	41.0	30.5	41.0
38-39	36.291460820105726	38.5	35.0	41.0	30.5	41.0
40-41	36.12960083797973	38.0	35.0	41.0	30.0	41.0
42-43	36.1089084192359	38.0	35.0	41.0	30.0	41.0
44-45	36.01109255768347	38.0	35.0	41.0	30.0	41.0
46-47	35.90593731795831	38.0	35.0	40.5	30.0	41.0
48-49	35.80987357939641	38.0	35.0	40.0	30.0	41.0
50-51	35.653990715647716	38.0	35.0	40.0	29.0	41.0
52-53	35.46545269358381	37.0	35.0	40.0	29.0	41.0
54-55	35.283179438379904	37.0	34.5	40.0	28.5	41.0
56-57	35.021526780254646	36.0	34.0	40.0	28.0	41.0
58-59	34.74392012417731	36.0	34.0	40.0	27.0	41.0
60-61	34.44262932422507	35.5	34.0	39.0	26.5	41.0
62-63	34.06457545616708	35.0	33.5	39.0	26.0	40.5
64-65	33.77478607274793	35.0	33.0	38.5	26.0	40.0
66-67	33.504505769251644	35.0	33.0	38.0	25.0	40.0
68-69	33.124370339280475	35.0	33.0	37.0	24.0	40.0
70-71	32.865974259983574	35.0	33.0	37.0	24.0	39.0
72-73	32.61577019238075	35.0	33.0	36.0	24.0	39.0
74-75	32.3374782454528	35.0	33.0	36.0	23.5	38.5
76-77	30.29592552256141	33.0	29.5	34.5	19.5	36.0
78-79	31.387088020435705	34.0	31.5	35.0	20.0	37.0
80-81	31.628111759576527	35.0	32.0	35.0	20.0	36.5
82-83	31.48982864110515	35.0	32.0	35.0	20.0	36.0
84-85	31.282004783286716	35.0	32.0	35.0	19.5	36.0
86	30.866391802560976	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	11.0
10	24.0
11	30.0
12	45.0
13	140.0
14	453.0
15	1393.0
16	3513.0
17	7110.0
18	13310.0
19	19463.0
20	22877.0
21	22470.0
22	20329.0
23	18292.0
24	18445.0
25	21089.0
26	25466.0
27	31609.0
28	38761.0
29	48255.0
30	61011.0
31	76078.0
32	96254.0
33	130191.0
34	324587.0
35	296530.0
36	233271.0
37	404210.0
38	557065.0
39	270317.0
40	1183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.98492287764266	23.762803975700038	29.459003759330482	13.793269387326824
2	31.14335749098159	24.84128678372814	29.95169676422594	14.063658961064334
3	29.833308608830627	24.270440228816227	31.53962493532432	14.356626227028826
4	27.007117038559368	26.29360407266833	32.22889582783062	14.470383060941677
5	24.33220324264868	31.20830453833323	29.471233342620096	14.988258876397989
6	22.548493192319246	39.28909215244284	27.376320197988996	10.786094457248922
7	86.20119473621368	4.438289450356214	7.1389287898139875	2.2215870236161215
8	86.6095470350497	3.8406680681238448	7.16161502863821	2.388169868188249
9	81.8029589802409	6.348818108466997	8.714627377622756	3.1335955336693457
10-11	40.45920637964534	27.33767760936974	19.96179159776973	12.241324413215187
12-13	30.1340188653986	26.49186443253648	26.919646572279373	16.45447012978555
14-15	16.223935248336524	32.11314897296828	34.77576082119119	16.88715495750401
16-17	20.772924136783185	25.688149244334774	33.300594473530914	20.23833214535113
18-19	23.83715839481292	28.0259534914013	29.967038016636575	18.169850097149205
20-21	26.452100195745697	27.463012746988735	29.328422202844646	16.756464854420923
22-23	21.62485210526125	25.081608950028766	30.999696069527715	22.293842875182268
24-25	19.962099146461924	26.129951986221815	34.55313175024152	19.35481711707474
26-27	17.705542027433342	34.33231178924593	29.31646398604814	18.64568219727259
28-29	16.70439866994236	29.115851783239684	35.58417969527352	18.59556985154444
30-31	21.792755600099863	29.430528368653192	30.705824248586183	18.070891782660766
32-33	25.122567199389245	28.612593576212376	28.942683778434684	17.322155445963695
34-35	20.162277162881406	28.945632627659844	29.764978525864844	21.127111683593906
36-37	22.386632124727278	29.79685504325582	29.650606594567602	18.1659062374493
38-39	19.91802922798042	31.024444693699593	28.358413627663463	20.699112450656525
40-41	20.405692907203516	27.22028446444918	29.557455523031777	22.816567105315528
42-43	21.02073601829372	27.22542233671878	28.16002663009852	23.593815014888975
44-45	19.43244602520452	31.69519030027607	28.166069057345165	20.706294617174244
46-47	20.542497801931408	30.5389519464214	28.17108029191798	20.747469959729212
48-49	21.453258026116313	26.803266528933094	30.981297421294673	20.762178023655924
50-51	19.046291505505124	31.126062399820537	28.65939525072455	21.168250843949792
52-53	21.437301676321283	27.36045430369167	30.158007663389764	21.04423635659728
54-55	21.15658208474595	27.56871904160591	31.633734835135808	19.640964038512333
56-57	22.028410262718946	26.11220461757225	29.399972501528698	22.459412618180107
58-59	22.31435818206159	28.27391010170816	28.84025197283441	20.571479743395845
60-61	23.37221713661313	29.42117527019057	27.032535033414263	20.17407255978204
62-63	22.57470719555393	26.297005199382006	31.305833655957944	19.82245394910612
64-65	19.391542049142664	30.217834929571353	30.20670890335373	20.183914117932257
66-67	18.617460081988863	29.963057974737588	26.604680529273207	24.81480141400034
68-69	17.871093679331644	32.09323067237381	26.12904743124478	23.906628217049775
70-71	19.625984608092512	30.065200322745216	28.665292949174866	21.643522119987406
72-73	21.195640768654638	30.86276453710304	27.35260276649094	20.588991927751383
74-75	20.255771965308508	32.69291443995383	25.1087275082405	21.942586086497165
76-77	23.945397443365813	28.78972353181682	26.609058575362095	20.655820449455277
78-79	18.74299422170281	30.11954598576592	29.66697903965207	21.470480752879197
80-81	16.809037589686625	29.259477022494472	30.537685569453537	23.39379981836536
82-83	18.674627956537943	28.083030910452678	31.33617242988794	21.906168703121438
84-85	17.30225523646876	27.766310030791054	30.03737621165139	24.894058521088795
86	18.486715705607153	27.644755932976096	30.572474753870594	23.29605360754616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2008.0
1	2391.0
2	6463.5
3	6642.5
4	3632.5
5	4579.5
6	5348.0
7	5670.0
8	6057.5
9	6435.0
10	6332.0
11	6203.5
12	6230.0
13	6366.0
14	6440.0
15	6483.0
16	6518.5
17	6954.0
18	7325.0
19	7284.5
20	8602.5
21	9889.0
22	10700.5
23	11290.0
24	11834.5
25	15051.5
26	19415.5
27	24304.5
28	31158.5
29	35037.0
30	38806.0
31	46436.0
32	52609.0
33	54519.5
34	61253.5
35	73399.0
36	78409.0
37	79654.5
38	84359.0
39	89664.0
40	106750.5
41	128393.0
42	137172.5
43	139550.0
44	139830.0
45	142506.0
46	152368.0
47	163032.0
48	160286.5
49	137708.0
50	121073.0
51	106224.0
52	86474.5
53	77627.0
54	71089.5
55	66624.5
56	62027.5
57	59305.0
58	52445.0
59	39560.0
60	29339.0
61	23746.5
62	21396.5
63	16946.5
64	13450.0
65	10446.0
66	5789.5
67	3796.5
68	2770.5
69	1631.0
70	829.5
71	482.0
72	444.5
73	429.0
74	331.0
75	179.0
76	103.5
77	50.5
78	31.5
79	22.0
80	23.0
81	27.0
82	21.0
83	10.0
84	5.0
85	2.5
86	3.0
87	3.5
88	2.5
89	3.5
90	3.0
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2763790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.810191590115004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.48555691462602	11.771963265526018
2	9.538397284814206	2.8253098250146222
3	3.000282495431714	1.3330427574543608
4	1.323027736558997	0.7837717702989978
5	0.7470743855434793	0.5532157390983186
6	0.5116861417449801	0.45469018759499386
7	0.37887562369888744	0.3927854403063388
8	0.28024434311236635	0.3320377930832057
9	0.2206032955384166	0.29404593651012406
>10	1.9084241095139491	6.238076630799069
>50	0.7051339367904723	7.714145042166893
>100	1.870164455060936	63.56410761217718
>500	0.02683621979549756	2.370039108996392
>1k	0.00344685391868776	0.828846410452583
>5k	0.0	0.0
>10k+	2.4620385133484E-4	0.5439224805209265
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14917	0.539729863701656	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4125	0.14925157121199512	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.618219908169579E-5	0.0	0.0	0.04848414676947235	0.0
2	3.618219908169579E-5	0.0	0.0	0.1667637555675359	0.0
3	3.618219908169579E-5	0.0	0.0	0.23413501025765343	0.0
4	3.618219908169579E-5	0.0	0.0	0.3464445562072372	0.0
5	3.618219908169579E-5	0.0	0.0	0.5710636481064046	0.0
6	3.618219908169579E-5	0.0	0.0	0.7453894832820149	0.0
7	3.618219908169579E-5	0.0	0.0	0.8662742104139606	0.0
8	3.618219908169579E-5	0.0	0.0	1.0974784625459966	0.0
9	3.618219908169579E-5	0.0	0.0	1.1721947036496985	0.0
10	3.618219908169579E-5	0.0	0.0	1.3151867544205602	0.0
11	3.618219908169579E-5	0.0	0.0	1.5799319051013283	0.0
12	3.618219908169579E-5	0.0	0.0	1.7729639372021753	0.0
13	3.618219908169579E-5	0.0	0.0	1.84518360656924	0.0
14	3.618219908169579E-5	0.0	0.0	1.8744188234272503	0.0
15	3.618219908169579E-5	0.0	0.0	1.9297414058231632	0.0
16	3.618219908169579E-5	0.0	0.0	2.038830736054476	0.0
17	3.618219908169579E-5	0.0	0.0	2.175707995180531	0.0
18	3.618219908169579E-5	0.0	0.0	2.337333878478466	0.0
19	3.618219908169579E-5	0.0	0.0	2.4493177846363148	0.0
20	3.618219908169579E-5	0.0	0.0	2.562857525354676	0.0
21	3.618219908169579E-5	0.0	0.0	2.7178620662206607	0.0
22	3.618219908169579E-5	0.0	0.0	2.878149208152573	0.0
23	3.618219908169579E-5	0.0	0.0	3.043465675756841	0.0
24	3.618219908169579E-5	0.0	0.0	3.160044721198065	0.0
25	3.618219908169579E-5	0.0	0.0	3.269495873420195	0.0
26	3.618219908169579E-5	0.0	0.0	3.3781510172625273	0.0
27	3.618219908169579E-5	0.0	0.0	3.4911118427955814	0.0
28	3.618219908169579E-5	0.0	0.0	3.6166640736090656	0.0
29	3.618219908169579E-5	0.0	0.0	3.7616823275285025	0.0
30	3.618219908169579E-5	0.0	0.0	3.9237062150163364	0.0
31	3.618219908169579E-5	0.0	0.0	4.077950929701605	0.0
32	3.618219908169579E-5	0.0	0.0	4.215841290401948	0.0
33	3.618219908169579E-5	0.0	0.0	4.358001150593931	0.0
34	3.618219908169579E-5	0.0	0.0	4.5095322003480725	0.0
35	3.618219908169579E-5	0.0	0.0	4.684581679505317	0.0
36	3.618219908169579E-5	0.0	0.0	4.840490775348344	0.0
37	3.618219908169579E-5	0.0	0.0	5.0099320136479255	0.0
38	3.618219908169579E-5	0.0	0.0	5.183136200652004	0.0
39	3.618219908169579E-5	0.0	0.0	5.406742190976884	0.0
40	3.618219908169579E-5	0.0	0.0	5.590873402103633	0.0
41	3.618219908169579E-5	0.0	0.0	5.782566692838457	0.0
42	3.618219908169579E-5	0.0	0.0	5.974296165772364	0.0
43	3.618219908169579E-5	0.0	0.0	6.146559615600317	0.0
44	3.618219908169579E-5	0.0	0.0	6.331740110500436	0.0
45	3.618219908169579E-5	0.0	0.0	6.513989847274938	0.0
46	3.618219908169579E-5	0.0	0.0	6.711689383057323	0.0
47	3.618219908169579E-5	0.0	0.0	6.924947264444838	0.0
48	3.618219908169579E-5	3.618219908169579E-5	0.0	7.1225020714308975	0.0
49	3.618219908169579E-5	3.618219908169579E-5	0.0	7.341657651268728	0.0
50	3.618219908169579E-5	3.618219908169579E-5	0.0	7.553070240503077	0.0
51	3.618219908169579E-5	3.618219908169579E-5	0.0	7.763361181565894	0.0
52	3.618219908169579E-5	3.618219908169579E-5	0.0	8.00162096251886	0.0
53	3.618219908169579E-5	3.618219908169579E-5	0.0	8.210790255410144	0.0
54	3.618219908169579E-5	3.618219908169579E-5	0.0	8.431176030016752	0.0
55	7.236439816339158E-5	3.618219908169579E-5	0.0	8.659992257009396	0.0
56	7.236439816339158E-5	3.618219908169579E-5	0.0	8.89347598768358	0.0
57	1.0854659724508736E-4	3.618219908169579E-5	0.0	9.110967186363652	0.0
58	1.0854659724508736E-4	3.618219908169579E-5	0.0	9.337865756804968	0.0
59	1.0854659724508736E-4	3.618219908169579E-5	0.0	9.57305005083599	0.0
60	1.0854659724508736E-4	3.618219908169579E-5	0.0	9.824226876861122	0.0
61	1.0854659724508736E-4	3.618219908169579E-5	0.0	10.082133591915449	0.0
62	1.0854659724508736E-4	3.618219908169579E-5	0.0	10.366055308109516	0.0
63	1.0854659724508736E-4	3.618219908169579E-5	0.0	10.682939007667008	0.0
64	1.0854659724508736E-4	3.618219908169579E-5	0.0	10.956114610733811	0.0
65	1.4472879632678316E-4	3.618219908169579E-5	0.0	11.225382536299792	0.0
66	1.4472879632678316E-4	3.618219908169579E-5	0.0	11.47775337489462	0.0
67	1.4472879632678316E-4	3.618219908169579E-5	0.0	11.737794839694766	0.0
68	1.4472879632678316E-4	3.618219908169579E-5	0.0	12.015420853248619	0.0
69	1.4472879632678316E-4	3.618219908169579E-5	0.0	12.358066278552277	0.0
70	1.8091099540847892E-4	3.618219908169579E-5	0.0	12.624656721386213	0.0
71	1.8091099540847892E-4	3.618219908169579E-5	0.0	12.892224083595353	0.0
72	2.1709319449017471E-4	3.618219908169579E-5	0.0	13.168547537982263	0.0
73	2.1709319449017471E-4	3.618219908169579E-5	0.0	13.44262769602611	0.0
74	2.1709319449017471E-4	3.618219908169579E-5	0.0	13.745979253127047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	100	0.0	72.0	1
CGTTTTT	8365	0.0	70.101616	1
CGTAAGG	390	0.0	69.74359	2
GCGTAAG	315	0.0	68.57143	1
AGGGCGA	1035	0.0	66.47343	6
AGTACGG	345	0.0	66.08696	2
ATAACGG	200	0.0	66.0	2
GCGATAA	190	0.0	65.26315	9
TACGGGA	1190	0.0	64.87395	4
CTATACG	25	1.2358325E-4	64.0	1
TAATGCG	50	3.0013325E-10	64.0	1
CGAGGGA	1180	0.0	63.389828	4
GGGCGAT	1670	0.0	62.994015	7
GGCGATA	395	0.0	62.784813	8
AGGGATT	5035	0.0	62.284008	6
GCGAACG	135	0.0	62.22222	1
TGCGCGG	450	0.0	62.22222	2
AGCGGGA	1440	0.0	61.944447	4
GAGGGAT	4090	0.0	61.613697	5
GATCGTT	280	0.0	61.428566	9
>>END_MODULE