FastQCFastQC Report
Thu 26 May 2016
SRR1547762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547762_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2150559
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149900.6970280750260747No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23670.11006440651012132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT351.2914825E-980.02
TTTCGCG1600.072.500011
CGAGGGA14550.072.302414
CGTTTTT75350.071.293951
TAGGGAC22750.068.747255
TACGGGA7800.068.205134
TTACCGG1550.067.096772
TTCGCGG2750.066.909092
TTAGGGA34050.065.550664
TAACGGG5000.064.83
ATAGCGG6250.064.639992
TGCGCGG3850.064.415582
TATAGCG3550.064.225351
ACGGGAC5300.064.150945
TAGGTCG503.0013325E-1064.01
ACGGGGT10250.063.6097565
CCGATCC11750.063.3191580
TAAGGGA33150.062.986434
GACGGGT2350.062.9787254
TAGCGGG16250.062.769233
Produced by FastQC (version 0.11.5)