##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547762_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2150559
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86822356419889	31.0	31.0	33.0	30.0	34.0
2	32.33828413914708	33.0	31.0	34.0	31.0	34.0
3	32.47214654422408	34.0	31.0	34.0	31.0	34.0
4	36.039471597849676	37.0	35.0	37.0	35.0	37.0
5	35.98741211006069	37.0	35.0	37.0	35.0	37.0
6	36.04180447967249	37.0	35.0	37.0	35.0	37.0
7	36.304676598038	37.0	36.0	37.0	35.0	37.0
8	36.277612471920094	37.0	37.0	37.0	35.0	37.0
9	38.19373056028688	39.0	39.0	39.0	37.0	39.0
10-11	37.60431543612614	39.0	37.5	39.0	35.0	39.0
12-13	36.87306160863292	39.0	35.0	39.0	33.0	39.0
14-15	37.96888088166844	40.0	36.0	41.0	33.0	41.0
16-17	38.14060135062559	40.0	35.0	41.0	34.0	41.0
18-19	38.032286721731424	40.0	36.0	41.0	34.0	41.0
20-21	37.83435516068148	40.0	35.0	41.0	34.0	41.0
22-23	37.663897386679466	39.5	35.0	41.0	33.0	41.0
24-25	37.6067110923253	39.0	35.0	41.0	33.0	41.0
26-27	37.584442928559504	39.5	35.0	41.0	33.0	41.0
28-29	37.57523648502552	40.0	35.0	41.0	33.0	41.0
30-31	37.403904287210906	39.0	35.0	41.0	33.0	41.0
32-33	37.079194758200074	39.0	35.0	41.0	32.5	41.0
34-35	36.79864095800208	39.0	35.0	41.0	31.5	41.0
36-37	36.61558994661388	39.0	35.0	41.0	31.0	41.0
38-39	36.52665818515093	39.0	35.0	41.0	31.0	41.0
40-41	36.37745744246031	39.0	35.0	41.0	31.0	41.0
42-43	36.32801587866224	39.0	35.0	41.0	31.0	41.0
44-45	36.237431756115505	39.0	35.0	41.0	30.0	41.0
46-47	36.13198219625688	38.0	35.0	41.0	30.0	41.0
48-49	36.016433866729535	38.0	35.0	40.5	30.0	41.0
50-51	35.84037359588832	38.0	35.0	40.0	30.0	41.0
52-53	35.659344384413544	38.0	35.0	40.0	29.0	41.0
54-55	35.456647783204275	37.0	35.0	40.0	29.0	41.0
56-57	35.16066566878658	37.0	34.5	40.0	28.0	41.0
58-59	34.8746388729628	36.0	34.0	40.0	27.0	41.0
60-61	34.56704094144824	36.0	34.0	39.0	26.5	41.0
62-63	34.18749776220973	35.5	33.5	39.0	26.0	41.0
64-65	33.868840380570816	35.0	33.5	39.0	26.0	40.0
66-67	33.57550432236456	35.0	33.0	38.0	25.0	40.0
68-69	33.147682997769415	35.0	33.0	37.0	24.0	40.0
70-71	32.88335428137522	35.0	33.0	37.0	24.0	39.0
72-73	32.61721975542173	35.0	33.0	36.0	23.5	39.0
74-75	32.30055046153117	35.0	33.0	36.0	22.5	38.5
76-77	30.251593887914723	33.0	29.5	34.5	19.0	36.0
78-79	31.344875448662417	34.0	31.5	35.0	20.0	37.0
80-81	31.56152539874516	35.0	32.0	35.0	20.0	36.5
82-83	31.4067563363758	35.0	32.0	35.0	19.5	36.0
84-85	31.20807264529827	35.0	32.0	35.0	19.0	36.0
86	30.779974880949556	34.0	31.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	5.0
11	9.0
12	29.0
13	69.0
14	261.0
15	798.0
16	1990.0
17	4723.0
18	10239.0
19	15319.0
20	17547.0
21	17315.0
22	15043.0
23	14030.0
24	14534.0
25	16282.0
26	19338.0
27	23705.0
28	29204.0
29	36285.0
30	46029.0
31	57171.0
32	71662.0
33	96047.0
34	232030.0
35	220459.0
36	183365.0
37	321724.0
38	456738.0
39	227757.0
40	842.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.363323210383907	26.24717573430908	31.0546234723158	14.334877582991211
2	30.440597072668083	25.46496050561738	30.712665869664583	13.381776552049956
3	27.999464325321927	24.685907245511515	33.520726471582506	13.793901957584051
4	26.310229107873813	26.847391771162755	32.60022161679824	14.242157504165196
5	24.211983953939416	30.935863652194616	30.991802596441204	13.860349797424762
6	22.94487154270122	39.01501888578737	26.922814021842694	11.117295549668714
7	89.20778272067868	2.9794113995477454	6.404706869237255	1.4080990105363302
8	90.38770849811607	2.3869607855445953	5.940920476955061	1.2844102393842716
9	86.9772463810572	4.795776353961923	6.469015730328718	1.7579615346521533
10-11	40.279666821510126	29.031777319292335	19.93235247207819	10.75620338711935
12-13	28.630928051729803	25.92370169802363	28.576081846626856	16.86928840361971
14-15	15.313088364467099	32.19555938711749	36.013938701519	16.47741354689641
16-17	21.0599430194661	27.06017365717472	33.04057224191478	18.839311081444407
18-19	22.946661774915267	28.222104113395634	31.58211423169511	17.249119879993994
20-21	25.247714663954813	28.738946478566735	30.574027497036816	15.439311360441636
22-23	20.5530515554328	25.503531872410846	32.66432122996858	21.279095342187777
24-25	18.386173083370416	28.26293070778342	35.720433617492006	17.630462591354153
26-27	17.452439110017444	35.441180641870325	29.694511985023432	17.411868263088806
28-29	15.807982947689414	30.17324797878133	35.8627222038549	18.15604686967435
30-31	21.796100455741975	29.55354863549431	31.347035817199153	17.303315091564563
32-33	24.09773458900686	29.31584299709982	29.147537919210777	17.438884494682547
34-35	19.28698538380021	29.725085431276238	30.304120928558575	20.683808256364973
36-37	21.556930081899637	29.931985125727778	31.0762457574984	17.434839034874187
38-39	19.51332188514707	31.27696101339233	29.047935908756745	20.161781192703852
40-41	20.025328298363355	27.700309547424645	29.600420169825615	22.673941984386385
42-43	20.195656106156584	26.93590364179732	30.18089715278679	22.687543099259308
44-45	18.94691101243909	32.19246716783869	28.65815353124467	20.202468288477554
46-47	20.721612380780996	31.03118770515015	28.634485266388875	19.612714647679972
48-49	20.55537653233415	28.02510882054387	31.72082235362992	19.698692293492066
50-51	18.839032084216242	31.431618476870433	29.509281075292513	20.220068363620808
52-53	21.218808691135653	27.730069251761986	31.01605210552233	20.03506995158003
54-55	21.447214421924716	28.614164968271037	30.71919905475739	19.21942155504685
56-57	21.969194986047814	26.62554247523551	30.372544998765438	21.03271753995124
58-59	22.612609093728654	28.205852524855164	29.37557165369562	19.805966727720563
60-61	22.89774426091077	29.288617517584964	28.24777185838659	19.56586636311768
62-63	22.15040368573938	27.349842529314472	31.209745931174176	19.290007853771975
64-65	19.520227066544095	30.96071765527009	30.660911883840434	18.85814339434538
66-67	18.762889090696884	30.135815850669523	27.3327306993205	23.76856435931309
68-69	17.74396796367828	32.53282053642797	27.118530577398715	22.604680922495035
70-71	19.193079566754502	30.514810335359314	29.729944633000073	20.562165464886107
72-73	21.64293097748074	30.08371311831017	28.49196418233585	19.78139172187324
74-75	20.689667198156386	32.24887110746555	26.35779813527553	20.70366355910254
76-77	24.125146066673828	29.383871821233456	27.22908322905812	19.261898883034597
78-79	19.11079863421557	29.700812672426096	30.594952289149006	20.593436404209324
80-81	17.265394718303472	29.326677389460137	31.27768175623175	22.13024613600464
82-83	18.45236517575198	27.841668143027	32.07331210164427	21.63265457957675
84-85	17.428003602784205	27.569785344182606	31.09863528505844	23.903575767974743
86	18.300916180397746	28.159422736135113	30.808826914304603	22.730834169162527
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1493.0
1	1818.0
2	5933.5
3	6076.0
4	2680.5
5	3487.5
6	4611.0
7	5180.0
8	5941.0
9	6742.0
10	6611.5
11	6358.0
12	5939.5
13	5835.5
14	6067.0
15	5913.5
16	5720.5
17	5970.0
18	6619.5
19	6923.5
20	7209.0
21	7551.0
22	8916.5
23	10879.0
24	12988.5
25	14863.0
26	18131.5
27	22054.0
28	26928.0
29	30786.0
30	33052.5
31	38896.5
32	44782.5
33	47275.0
34	51262.5
35	59580.0
36	64094.0
37	65564.5
38	69836.5
39	74476.0
40	85069.0
41	101718.5
42	111538.0
43	113463.0
44	116681.0
45	119200.5
46	120458.5
47	119858.5
48	115485.5
49	102161.5
50	90654.0
51	81023.0
52	66772.0
53	58630.0
54	51090.5
55	44877.0
56	39285.0
57	35889.0
58	32395.5
59	25098.5
60	17898.0
61	12780.0
62	9912.0
63	6942.0
64	5119.0
65	4109.0
66	2675.0
67	1572.5
68	760.0
69	583.5
70	421.0
71	301.0
72	285.5
73	194.5
74	173.5
75	136.5
76	46.5
77	33.0
78	15.0
79	12.0
80	12.0
81	11.0
82	7.5
83	7.0
84	6.5
85	2.5
86	1.0
87	2.0
88	3.5
89	3.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2150559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.056072917104316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94094085174419	12.674815024604236
2	8.750775702535588	2.810061855222723
3	2.6844714035740513	1.2930620579899899
4	1.3938598117820726	0.8951965909677704
5	0.8475946864896942	0.680452104521435
6	0.6083716058769112	0.586083531879332
7	0.46558433290440476	0.5232819198722146
8	0.37318272338203323	0.47934792144203964
9	0.3212120337867375	0.464166345269811
>10	2.780656745397767	10.168168661720665
>50	1.0386426981012415	12.150845270799874
>100	1.7868256380103031	55.49226983650898
>500	0.005838345492583246	0.6326573404721622
>1k	0.0017515036477749736	0.4470034861522456
>5k	0.0	0.0
>10k+	2.919172746291623E-4	0.7025880525765085
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14990	0.6970280750260747	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2367	0.11006440651012132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.64995380270897E-5	0.0	0.0	0.024272758850140824	0.0
2	4.64995380270897E-5	0.0	0.0	0.11624884506772425	0.0
3	4.64995380270897E-5	0.0	0.0	0.18520765996189828	0.0
4	4.64995380270897E-5	0.0	0.0	0.3028514911704352	0.0
5	4.64995380270897E-5	0.0	0.0	0.5765477719978852	0.0
6	4.64995380270897E-5	0.0	0.0	0.7707298427990118	0.0
7	4.64995380270897E-5	0.0	0.0	0.9073919850606285	0.0
8	4.64995380270897E-5	0.0	0.0	1.1550020250548811	0.0
9	4.64995380270897E-5	0.0	0.0	1.2556270253455033	0.0
10	4.64995380270897E-5	0.0	0.0	1.4273033197415184	0.0
11	4.64995380270897E-5	0.0	0.0	1.811296504769225	0.0
12	4.64995380270897E-5	0.0	0.0	2.0695084394336543	0.0
13	4.64995380270897E-5	0.0	0.0	2.1607870325808314	0.0
14	4.64995380270897E-5	0.0	0.0	2.1973356694701236	0.0
15	4.64995380270897E-5	0.0	0.0	2.2548090984716067	0.0
16	4.64995380270897E-5	0.0	0.0	2.3699419546266807	0.0
17	4.64995380270897E-5	0.0	0.0	2.5203214606062887	0.0
18	4.64995380270897E-5	0.0	0.0	2.6884172905742183	0.0
19	4.64995380270897E-5	0.0	0.0	2.823591447618968	0.0
20	9.29990760541794E-5	0.0	0.0	2.95537113838774	0.0
21	9.29990760541794E-5	0.0	0.0	3.1105400967841383	0.0
22	9.29990760541794E-5	0.0	0.0	3.2924462895461133	0.0
23	9.29990760541794E-5	0.0	0.0	3.4743524823080882	0.0
24	9.29990760541794E-5	0.0	0.0	3.6101776328852173	0.0
25	9.29990760541794E-5	0.0	0.0	3.727030971947294	0.0
26	9.29990760541794E-5	0.0	0.0	3.8404433451953657	0.0
27	9.29990760541794E-5	0.0	0.0	3.9755710027020883	0.0
28	9.29990760541794E-5	0.0	0.0	4.101538251217474	0.0
29	9.29990760541794E-5	0.0	0.0	4.257032706380062	0.0
30	9.29990760541794E-5	0.0	0.0	4.421687570533987	0.0
31	9.29990760541794E-5	0.0	0.0	4.575787039555762	0.0
32	9.29990760541794E-5	0.0	0.0	4.715471651789139	0.0
33	9.29990760541794E-5	0.0	0.0	4.854877266794355	0.0
34	9.29990760541794E-5	0.0	0.0	5.012929196548432	0.0
35	9.29990760541794E-5	0.0	0.0	5.195951378223057	0.0
36	9.29990760541794E-5	0.0	0.0	5.360513243300928	0.0
37	9.29990760541794E-5	0.0	0.0	5.5406059540798465	0.0
38	9.29990760541794E-5	0.0	0.0	5.724883623281203	0.0
39	9.29990760541794E-5	0.0	0.0	5.987001519139907	0.0
40	9.29990760541794E-5	0.0	0.0	6.175836143067919	0.0
41	9.29990760541794E-5	0.0	0.0	6.379736617316707	0.0
42	9.29990760541794E-5	0.0	0.0	6.585357574472497	0.0
43	9.29990760541794E-5	0.0	0.0	6.771727722885073	0.0
44	9.29990760541794E-5	0.0	0.0	6.958795364368055	0.0
45	1.394986140812691E-4	0.0	0.0	7.149768967045312	0.0
46	1.394986140812691E-4	0.0	0.0	7.357389404336268	0.0
47	1.394986140812691E-4	0.0	0.0	7.586678626347847	0.0
48	1.394986140812691E-4	0.0	0.0	7.798484022061241	0.0
49	1.394986140812691E-4	0.0	0.0	8.027215249616495	0.0
50	1.394986140812691E-4	0.0	0.0	8.24604207557198	0.0
51	1.394986140812691E-4	0.0	0.0	8.479423256929943	0.0
52	1.394986140812691E-4	0.0	0.0	8.720151365296186	0.0
53	1.394986140812691E-4	0.0	0.0	8.959344988907535	0.0
54	1.394986140812691E-4	0.0	0.0	9.195934638389367	0.0
55	1.394986140812691E-4	0.0	0.0	9.432105792028956	0.0
56	1.394986140812691E-4	0.0	0.0	9.670322925341736	0.0
57	1.394986140812691E-4	0.0	0.0	9.91951394962891	0.0
58	1.394986140812691E-4	0.0	0.0	10.168379477149895	0.0
59	1.394986140812691E-4	0.0	0.0	10.422499452467939	0.0
60	1.394986140812691E-4	0.0	0.0	10.68071138713237	0.0
61	1.859981521083588E-4	0.0	0.0	10.964079571869453	0.0
62	1.859981521083588E-4	0.0	0.0	11.270558027006002	0.0
63	1.859981521083588E-4	0.0	0.0	11.590846844936596	0.0
64	1.859981521083588E-4	0.0	0.0	11.877191000107414	0.0
65	1.859981521083588E-4	0.0	0.0	12.14856230403351	0.0
66	1.859981521083588E-4	0.0	0.0	12.415795149075194	0.0
67	1.859981521083588E-4	0.0	0.0	12.695582869384193	0.0
68	1.859981521083588E-4	0.0	0.0	13.019870647585115	0.0
69	1.859981521083588E-4	0.0	0.0	13.458686787946762	0.0
70	2.324976901354485E-4	0.0	0.0	13.756097833168027	0.0
71	2.324976901354485E-4	0.0	0.0	14.065087263358038	0.0
72	2.324976901354485E-4	0.0	0.0	14.36338179980182	0.0
73	2.789972281625382E-4	0.0	0.0	14.667860774803202	0.0
74	2.789972281625382E-4	0.0	0.0	15.002331951832058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	35	1.2914825E-9	80.0	2
TTTCGCG	160	0.0	72.50001	1
CGAGGGA	1455	0.0	72.30241	4
CGTTTTT	7535	0.0	71.29395	1
TAGGGAC	2275	0.0	68.74725	5
TACGGGA	780	0.0	68.20513	4
TTACCGG	155	0.0	67.09677	2
TTCGCGG	275	0.0	66.90909	2
TTAGGGA	3405	0.0	65.55066	4
TAACGGG	500	0.0	64.8	3
ATAGCGG	625	0.0	64.63999	2
TGCGCGG	385	0.0	64.41558	2
TATAGCG	355	0.0	64.22535	1
ACGGGAC	530	0.0	64.15094	5
TAGGTCG	50	3.0013325E-10	64.0	1
ACGGGGT	1025	0.0	63.609756	5
CCGATCC	1175	0.0	63.31915	80
TAAGGGA	3315	0.0	62.98643	4
GACGGGT	235	0.0	62.978725	4
TAGCGGG	1625	0.0	62.76923	3
>>END_MODULE