FastQCFastQC Report
Thu 26 May 2016
SRR1547761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547761_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3748276
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298950.7975666679828273No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39940.10655565385259784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG2850.072.982461
TACGGGA13850.072.7797854
CGTTTTT126400.072.689871
TGTCGCG451.1823431E-1071.111111
TAGGGTA35050.067.44655
CGGGTAC4400.065.4545446
CGTAAGG3500.065.142862
CGAGGGA23600.064.915254
CGTGCGG2500.064.02
TTAGCGG10900.063.8532072
CGTTAGG9350.063.3155062
GGCGATT7650.063.267988
TAGCGGG30600.062.875823
GCACCGA4400.062.7272729
ACGGGAT17600.061.818185
GTCCGCG650.061.538461
GTAGGGT30800.061.2987024
GCGGGTC6200.061.290325
GGGCGAT23450.061.236677
CGGGATC10950.061.0045666
Produced by FastQC (version 0.11.5)