##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547761_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3748276
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7549918949405	31.0	31.0	33.0	30.0	34.0
2	32.19862278017948	33.0	31.0	34.0	31.0	34.0
3	32.3525530137055	34.0	31.0	34.0	30.0	34.0
4	35.94651407740518	37.0	35.0	37.0	35.0	37.0
5	35.895867326739015	37.0	35.0	37.0	35.0	37.0
6	35.95155532836963	37.0	35.0	37.0	35.0	37.0
7	36.2515497791518	37.0	35.0	37.0	35.0	37.0
8	36.220263662547794	37.0	36.0	37.0	35.0	37.0
9	38.103023630063525	39.0	39.0	39.0	37.0	39.0
10-11	37.45766800523761	39.0	37.0	39.0	34.5	39.0
12-13	36.88850367475607	39.0	35.0	39.0	33.0	39.0
14-15	37.96799261847313	40.0	36.0	41.0	33.0	41.0
16-17	38.13303129225275	40.0	36.0	41.0	34.0	41.0
18-19	38.03930020094572	40.0	36.0	41.0	34.0	41.0
20-21	37.86459561675821	40.0	35.0	41.0	33.5	41.0
22-23	37.67303408286904	39.5	35.0	41.0	33.0	41.0
24-25	37.60891567216502	39.0	35.0	41.0	33.0	41.0
26-27	37.59999450414004	40.0	35.0	41.0	33.0	41.0
28-29	37.55663256921315	40.0	35.0	41.0	33.0	41.0
30-31	37.371827474817756	39.0	35.0	41.0	33.0	41.0
32-33	37.060289717192646	39.0	35.0	41.0	32.5	41.0
34-35	36.799436327527644	39.0	35.0	41.0	31.0	41.0
36-37	36.6224625667907	39.0	35.0	41.0	31.0	41.0
38-39	36.52718489780368	39.0	35.0	41.0	31.0	41.0
40-41	36.39636982442062	39.0	35.0	41.0	30.0	41.0
42-43	36.37783543687818	39.0	35.0	41.0	30.0	41.0
44-45	36.282510412787104	39.0	35.0	41.0	30.0	41.0
46-47	36.17401706811345	38.0	35.0	41.0	30.0	41.0
48-49	36.06892115201762	38.0	35.0	40.5	30.0	41.0
50-51	35.908150173573134	38.0	35.0	40.0	29.5	41.0
52-53	35.738809255241605	38.0	35.0	40.0	29.0	41.0
54-55	35.55320232554914	37.5	35.0	40.0	29.0	41.0
56-57	35.26947988888759	37.0	34.5	40.0	28.0	41.0
58-59	34.97028820716511	37.0	34.0	40.0	27.0	41.0
60-61	34.64115716131896	36.0	34.0	40.0	26.5	41.0
62-63	34.24340723575318	36.0	33.5	39.0	26.0	41.0
64-65	33.845574205314655	35.0	33.0	39.0	25.0	40.5
66-67	33.49015400680206	35.0	33.0	38.5	23.5	40.0
68-69	32.988529926825024	35.0	33.0	37.5	22.0	40.0
70-71	32.63621342185047	35.0	33.0	37.0	21.0	39.5
72-73	32.30583140088937	35.0	32.5	36.5	20.0	39.0
74-75	31.949088460935105	35.0	32.0	36.0	19.5	38.5
76-77	29.895609875046553	33.0	29.0	34.5	16.0	36.0
78-79	30.945931542928	34.0	30.5	35.0	17.0	37.0
80-81	31.08052488664122	35.0	31.0	35.0	17.0	37.0
82-83	30.852786454359283	35.0	31.0	35.0	14.0	36.0
84-85	30.591790465803477	34.0	31.0	35.0	11.5	36.0
86	30.15712957103479	34.0	30.0	35.0	10.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	4.0
10	12.0
11	39.0
12	52.0
13	121.0
14	448.0
15	1319.0
16	3494.0
17	8448.0
18	17377.0
19	26235.0
20	29226.0
21	27461.0
22	24412.0
23	24051.0
24	26124.0
25	30896.0
26	37645.0
27	47056.0
28	58513.0
29	74372.0
30	93808.0
31	114555.0
32	138364.0
33	174751.0
34	372352.0
35	366389.0
36	326082.0
37	525990.0
38	783669.0
39	413659.0
40	1345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.928512201342695	24.228338574854146	31.3164772284645	13.526671995338656
2	29.52079836170015	25.427103020161802	31.845733878721845	13.206364739416202
3	28.611046785242067	24.403859267567277	33.8311533088812	13.153940638309452
4	25.632557474422907	27.448352255810406	32.65063725296643	14.268453016800256
5	24.271371691945845	31.838877393233584	30.175739459954386	13.714011454866185
6	22.462860258956386	40.70588718653589	26.429083664063157	10.402168890444567
7	88.76056619096353	3.170257473035604	6.540660292891985	1.5285160431088853
8	89.35091759518242	2.717409283627993	6.2982021601397555	1.6334709610498266
9	85.21197478520793	4.959906901199378	7.670913241180745	2.1572050724119567
10-11	35.71168451842927	28.70937198861557	23.95906811558167	11.619875377373491
12-13	28.19728323101074	26.13232056550798	28.755086338359288	16.91530986512199
14-15	15.676847169205255	32.62581250686982	34.54333405544309	17.15400626848183
16-17	20.72735572300439	27.87377450326497	32.46087001063956	18.937999763091085
18-19	21.96938005632456	29.446750986320108	30.559289123853205	18.024579833502123
20-21	24.390946664546583	29.726039384506368	30.50048875803169	15.382525192915356
22-23	20.314205784205857	25.82576629895984	32.65741370165911	21.20261421517519
24-25	17.667028788701792	29.734896789884203	34.081841892112536	18.516232529301472
26-27	17.267818591800605	35.952835383520316	28.638899590104888	18.14044643457419
28-29	15.909620849691967	31.12684338079693	34.41671584483107	18.54681992468004
30-31	20.51189666929543	31.38146710647775	30.40136318670237	17.705273037524453
32-33	23.421060775673936	30.171724280709316	28.54525653927299	17.861958404343756
34-35	18.709961593009695	29.925771207883304	30.877288652169693	20.486978546937312
36-37	21.126992782815353	30.952109716573702	29.926798346759952	17.994099153851
38-39	18.73170492247636	31.83447536947653	29.07580711772559	20.358012590321522
40-41	19.635573794459106	28.540534368333603	30.167682422532387	21.65620941467491
42-43	19.471591739775835	28.49879517943716	29.164954768538927	22.864658312248086
44-45	18.85446802743448	32.20621960602688	28.276332906114703	20.662979460423937
46-47	19.74264968748299	31.956571501138125	28.079802554561084	20.220976256817803
48-49	20.284538811976493	28.869525616576798	30.908289570992103	19.93764600045461
50-51	17.92945610195194	31.561616593868752	29.088706381280353	21.420220922898956
52-53	20.693726929393673	28.17480623091789	30.831307513107358	20.300159326581074
54-55	20.68514965280038	28.903754686154382	30.16105270796494	20.250042953080296
56-57	20.63746106209895	27.45917056268002	29.815573879831685	22.087794495389346
58-59	21.88258015151499	29.25863783776862	28.792196732577857	20.066585278138536
60-61	21.66677427169184	30.917467123552267	27.555348112038708	19.860410492717186
62-63	21.036324432885944	29.08767924240371	30.433564657458522	19.442431667251824
64-65	18.83971457811538	32.48870947603645	29.25474271371692	19.416833232131253
66-67	17.703458870157906	31.18548367302728	26.81209974932476	24.298957707490057
68-69	16.976938197720763	33.66386306664717	26.738812723502754	22.62038601212931
70-71	18.1292012647948	31.86805080522352	28.777883485634465	21.22486444434721
72-73	20.492754535685208	31.202051289712923	28.335106593004355	19.97008758159751
74-75	19.4408442708061	33.05859813951801	26.477279154469947	21.023278435205945
76-77	22.71538968848612	30.619916996507197	27.0231301003448	19.641563214661886
78-79	18.12239813717026	31.411827730935503	29.425754666945554	21.040019464948685
80-81	16.185987904839454	30.832734835961915	30.859093620640525	22.1221836385581
82-83	17.874577539113982	29.33888806480633	30.749229779237176	22.037304616842516
84-85	16.458259743946282	29.3601511734995	29.874267529925756	24.307321552628462
86	16.87501133854604	29.89174223029468	30.245878371816804	22.98736805934248
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2486.0
1	3172.5
2	12283.0
3	12455.5
4	4774.0
5	7316.5
6	11897.5
7	14506.0
8	16004.5
9	17388.5
10	16168.5
11	14438.5
12	12889.5
13	11854.5
14	11744.0
15	11286.5
16	10962.5
17	11036.5
18	11235.0
19	11787.5
20	12650.5
21	13219.0
22	13494.5
23	14375.5
24	17427.5
25	20661.0
26	26339.5
27	32961.5
28	40049.5
29	45407.0
30	51407.5
31	61917.5
32	73162.5
33	80853.5
34	86420.5
35	98657.0
36	106282.0
37	115506.5
38	132683.5
39	152536.0
40	173731.5
41	192468.0
42	204724.0
43	207539.0
44	210307.5
45	212721.5
46	215279.0
47	211982.5
48	201740.0
49	179629.5
50	161553.0
51	143863.0
52	114364.5
53	92582.5
54	77733.0
55	69561.5
56	59573.5
57	52881.0
58	47360.5
59	35890.5
60	24663.5
61	16849.0
62	12023.5
63	8109.0
64	6483.0
65	5170.5
66	3234.0
67	2024.0
68	1187.0
69	709.0
70	426.5
71	371.0
72	316.5
73	228.0
74	140.0
75	79.0
76	95.0
77	79.5
78	30.5
79	20.0
80	30.5
81	29.5
82	13.0
83	6.5
84	5.0
85	4.0
86	3.0
87	3.5
88	3.0
89	2.0
90	1.0
91	0.0
92	1.0
93	2.0
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3748276.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.014482533068115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52747756309893	12.095334702009417
2	10.280237794203174	3.2926537718210684
3	3.7022100670276434	1.778669353564894
4	1.8828218695237469	1.206096717694668
5	1.1691000019489346	0.9361265780310556
6	0.8195635782533887	0.7874931965206616
7	0.6055554815723397	0.6788360377710719
8	0.4837627071298022	0.619776753878397
9	0.3960443411858597	0.5708200665817277
>10	2.8161690673442963	9.290880034293233
>50	0.7328953713115197	8.600668976339664
>100	1.5566394823077319	55.75893368592817
>500	0.02245715206939382	2.185635730151487
>1k	0.004896672255732488	1.3896963562091769
>5k	0.0	0.0
>10k+	1.688507674390513E-4	0.8083780392052098
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29895	0.7975666679828273	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3994	0.10655565385259784	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.667893186094087E-5	0.0	0.0	0.08465225079476538	0.0
2	2.667893186094087E-5	0.0	0.0	0.30440661253333534	0.0
3	2.667893186094087E-5	0.0	0.0	0.43358600060401103	0.0
4	2.667893186094087E-5	0.0	0.0	0.6260211361169775	0.0
5	2.667893186094087E-5	0.0	0.0	1.1127782479198436	0.0
6	2.667893186094087E-5	0.0	0.0	1.4562161377657354	0.0
7	2.667893186094087E-5	0.0	0.0	1.7128941411998475	0.0
8	2.667893186094087E-5	0.0	0.0	2.216859164053021	0.0
9	2.667893186094087E-5	0.0	0.0	2.3864571338930216	0.0
10	2.667893186094087E-5	0.0	0.0	2.721944702044353	0.0
11	2.667893186094087E-5	0.0	0.0	3.335239987663662	0.0
12	2.667893186094087E-5	0.0	0.0	3.733983303257284	0.0
13	2.667893186094087E-5	0.0	0.0	3.8777027091921727	0.0
14	2.667893186094087E-5	0.0	0.0	3.937623590151846	0.0
15	2.667893186094087E-5	0.0	0.0	4.032920734759126	0.0
16	2.667893186094087E-5	0.0	0.0	4.245952005668739	0.0
17	2.667893186094087E-5	0.0	0.0	4.522025592565756	0.0
18	2.667893186094087E-5	0.0	0.0	4.828833308966575	0.0
19	2.667893186094087E-5	0.0	0.0	5.042851700355043	0.0
20	2.667893186094087E-5	0.0	0.0	5.2556428608779076	0.0
21	2.667893186094087E-5	0.0	0.0	5.5216584904633494	0.0
22	2.667893186094087E-5	0.0	0.0	5.820809353420079	0.0
23	2.667893186094087E-5	0.0	0.0	6.120893978991942	0.0
24	2.667893186094087E-5	0.0	0.0	6.3294698682807775	0.0
25	2.667893186094087E-5	0.0	0.0	6.517369585377384	0.0
26	2.667893186094087E-5	0.0	0.0	6.689341980153009	0.0
27	1.0671572744376348E-4	0.0	0.0	6.8832444569183275	0.0
28	1.0671572744376348E-4	0.0	0.0	7.080748589484872	0.0
29	1.0671572744376348E-4	0.0	0.0	7.318511230229578	0.0
30	1.0671572744376348E-4	0.0	0.0	7.5685461796303155	0.0
31	1.0671572744376348E-4	0.0	0.0	7.790755003100092	0.0
32	1.0671572744376348E-4	0.0	0.0	7.986044784322179	0.0
33	1.0671572744376348E-4	0.0	0.0	8.192272927607252	0.0
34	1.0671572744376348E-4	0.0	0.0	8.410506590229748	0.0
35	1.0671572744376348E-4	0.0	0.0	8.662729212043084	0.0
36	1.0671572744376348E-4	0.0	0.0	8.889153306746888	0.0
37	1.0671572744376348E-4	0.0	0.0	9.127582920788116	0.0
38	1.0671572744376348E-4	0.0	0.0	9.376230565732087	0.0
39	1.0671572744376348E-4	0.0	0.0	9.749308748875483	0.0
40	1.0671572744376348E-4	0.0	0.0	10.008227782585914	0.0
41	1.0671572744376348E-4	0.0	0.0	10.260930625172746	0.0
42	1.0671572744376348E-4	0.0	0.0	10.527799980577738	0.0
43	1.0671572744376348E-4	0.0	0.0	10.77994256559549	0.0
44	1.0671572744376348E-4	0.0	0.0	11.026589290649888	0.0
45	1.0671572744376348E-4	0.0	0.0	11.258269134930298	0.0
46	1.0671572744376348E-4	0.0	0.0	11.521830302784533	0.0
47	1.0671572744376348E-4	0.0	0.0	11.788059363824862	0.0
48	1.0671572744376348E-4	0.0	0.0	12.05989100055599	0.0
49	1.0671572744376348E-4	0.0	0.0	12.338125580933742	0.0
50	1.0671572744376348E-4	0.0	0.0	12.618707907315256	0.0
51	1.0671572744376348E-4	0.0	0.0	12.896595661578816	0.0
52	1.3339465930470434E-4	0.0	0.0	13.22135829912205	0.0
53	1.3339465930470434E-4	0.0	0.0	13.512478803588637	0.0
54	1.3339465930470434E-4	0.0	0.0	13.803839418441973	0.0
55	1.3339465930470434E-4	0.0	0.0	14.093625976315511	0.0
56	1.3339465930470434E-4	0.0	0.0	14.39130949801989	0.0
57	1.6007359116564521E-4	0.0	0.0	14.676053737771712	0.0
58	1.6007359116564521E-4	0.0	0.0	14.981901012625539	0.0
59	1.6007359116564521E-4	0.0	0.0	15.269259787699731	0.0
60	1.6007359116564521E-4	0.0	0.0	15.588713317802638	0.0
61	1.867525230265861E-4	0.0	0.0	15.901096930962394	0.0
62	1.867525230265861E-4	0.0	0.0	16.248189834473234	0.0
63	1.867525230265861E-4	0.0	0.0	16.611530207487387	0.0
64	2.1343145488752696E-4	0.0	0.0	16.94552375545451	0.0
65	2.1343145488752696E-4	0.0	0.0	17.265324111671607	0.0
66	2.4011038674846784E-4	0.0	0.0	17.58101591238212	0.0
67	2.4011038674846784E-4	0.0	0.0	17.905004860901386	0.0
68	2.4011038674846784E-4	0.0	0.0	18.268238518188095	0.0
69	2.4011038674846784E-4	0.0	0.0	18.754515409217465	0.0
70	2.667893186094087E-4	0.0	0.0	19.102515396411576	0.0
71	2.667893186094087E-4	0.0	0.0	19.444512623936976	0.0
72	2.667893186094087E-4	0.0	0.0	19.78968464435383	0.0
73	2.667893186094087E-4	0.0	0.0	20.149289966907453	0.0
74	2.9346825047034956E-4	0.0	0.0	20.519940367251504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	285	0.0	72.98246	1
TACGGGA	1385	0.0	72.779785	4
CGTTTTT	12640	0.0	72.68987	1
TGTCGCG	45	1.1823431E-10	71.11111	1
TAGGGTA	3505	0.0	67.4465	5
CGGGTAC	440	0.0	65.454544	6
CGTAAGG	350	0.0	65.14286	2
CGAGGGA	2360	0.0	64.91525	4
CGTGCGG	250	0.0	64.0	2
TTAGCGG	1090	0.0	63.853207	2
CGTTAGG	935	0.0	63.315506	2
GGCGATT	765	0.0	63.26798	8
TAGCGGG	3060	0.0	62.87582	3
GCACCGA	440	0.0	62.727272	9
ACGGGAT	1760	0.0	61.81818	5
GTCCGCG	65	0.0	61.53846	1
GTAGGGT	3080	0.0	61.298702	4
GCGGGTC	620	0.0	61.29032	5
GGGCGAT	2345	0.0	61.23667	7
CGGGATC	1095	0.0	61.004566	6
>>END_MODULE