FastQCFastQC Report
Thu 26 May 2016
SRR1547760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547760_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1716363
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85650.4990203121367683No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA22650.13196509130061648No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAA20770.12101169740899798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG1300.073.846151
ACGGGTA503.6379788E-1272.05
ATCGTAG451.1823431E-1071.111111
TAACGGG4850.069.278353
TACGGGA7750.069.161294
CGTTTTT44850.068.940911
ATAGCGG3850.068.5714342
TAGCGGG12300.067.642273
ACGGGAT10650.066.103295
TAGCACG800.065.01
CGGGTAG1050.064.761916
ACGGGAC5400.064.444445
ATAGGGA21900.063.7442934
CGGGATC6850.063.6496356
CGTTAGG2350.062.9787252
AGGGATT39400.062.335036
GCGAGGG16800.061.9047663
CGAGGGA16200.061.72844
TGCACGG3650.061.3698652
CGGGATA5550.061.2612656
Produced by FastQC (version 0.11.5)