##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547760_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1716363
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.767081322540744	31.0	31.0	33.0	30.0	34.0
2	32.21247370165868	33.0	31.0	34.0	31.0	34.0
3	32.384887113040776	34.0	31.0	34.0	31.0	34.0
4	35.986746393391144	37.0	35.0	37.0	35.0	37.0
5	35.93276888397151	37.0	35.0	37.0	35.0	37.0
6	35.993631300604825	37.0	35.0	37.0	35.0	37.0
7	36.19535378005702	37.0	35.0	37.0	35.0	37.0
8	36.13148442374952	37.0	35.0	37.0	35.0	37.0
9	37.99229941451779	39.0	39.0	39.0	35.0	39.0
10-11	37.53095178584017	39.0	37.0	39.0	35.0	39.0
12-13	36.7454777922852	39.0	35.0	39.0	33.0	39.0
14-15	37.80151459801918	40.0	35.0	41.0	33.0	41.0
16-17	37.97681084945317	40.0	35.0	41.0	33.5	41.0
18-19	37.87028618072051	40.0	36.0	41.0	33.5	41.0
20-21	37.69186588151807	40.0	35.0	41.0	33.5	41.0
22-23	37.499561281617005	39.0	35.0	41.0	33.0	41.0
24-25	37.40160239995852	39.0	35.0	41.0	33.0	41.0
26-27	37.38941995370443	39.0	35.0	41.0	33.0	41.0
28-29	37.382389389657085	39.0	35.0	41.0	33.0	41.0
30-31	37.23591046882274	39.0	35.0	41.0	33.0	41.0
32-33	36.94745750170564	39.0	35.0	41.0	32.0	41.0
34-35	36.665316427818595	39.0	35.0	41.0	31.0	41.0
36-37	36.480015591107474	39.0	35.0	41.0	31.0	41.0
38-39	36.40099297176646	39.0	35.0	41.0	31.0	41.0
40-41	36.24099214443565	38.5	35.0	41.0	30.0	41.0
42-43	36.202745864365525	38.0	35.0	41.0	30.0	41.0
44-45	36.08807723074897	38.0	35.0	41.0	30.0	41.0
46-47	35.984845571711816	38.0	35.0	40.5	30.0	41.0
48-49	35.89713568749734	38.0	35.0	40.0	30.0	41.0
50-51	35.718320658275665	38.0	35.0	40.0	29.0	41.0
52-53	35.528051758281904	37.0	35.0	40.0	29.0	41.0
54-55	35.32723409908044	37.0	34.5	40.0	28.5	41.0
56-57	35.02307553821657	36.5	34.0	40.0	27.5	41.0
58-59	34.74361658926462	36.0	34.0	40.0	27.0	41.0
60-61	34.44410011168966	35.5	34.0	39.0	26.5	41.0
62-63	34.07668803161103	35.0	33.5	39.0	26.0	41.0
64-65	33.83730539518738	35.0	33.0	38.5	26.0	40.0
66-67	33.580009881359594	35.0	33.0	38.0	25.5	40.0
68-69	33.209250607243334	35.0	33.0	37.0	25.0	40.0
70-71	32.992643164645244	35.0	33.0	37.0	25.0	39.0
72-73	32.76621145993009	35.0	33.0	36.0	25.0	39.0
74-75	32.483182170671355	35.0	33.0	36.0	24.0	38.5
76-77	30.44191001553867	33.0	29.5	34.5	19.5	36.0
78-79	31.56689144429238	34.0	31.5	35.0	22.5	37.0
80-81	31.839379839812437	35.0	32.0	35.0	23.0	36.5
82-83	31.705367396057827	35.0	32.0	35.0	23.0	36.0
84-85	31.542908172688414	35.0	32.0	35.0	22.5	36.0
86	31.1357772219513	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	9.0
10	21.0
11	34.0
12	41.0
13	84.0
14	249.0
15	809.0
16	1816.0
17	3724.0
18	6979.0
19	11033.0
20	13274.0
21	13822.0
22	12803.0
23	12116.0
24	12291.0
25	13764.0
26	16165.0
27	19691.0
28	23977.0
29	29628.0
30	36232.0
31	45613.0
32	57346.0
33	79131.0
34	193171.0
35	175751.0
36	151625.0
37	262436.0
38	350518.0
39	171551.0
40	654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.545467945883246	24.65917757490694	27.94933239646858	13.84602208274124
2	30.659423443642165	24.100146647300132	31.335387677315346	13.905042231742353
3	29.715508898758596	24.894442492642874	31.44958263490882	13.940465973689713
4	27.368161630144673	27.083839490830318	30.47018608534442	15.077812793680593
5	24.32416685747712	31.31715144174047	29.353464273000522	15.005217427781886
6	22.61980711539459	40.742546885478184	25.527292303551175	11.110353695576054
7	87.09189140059533	4.120981400787596	6.347841336593715	2.4392858620233597
8	87.29866584166636	3.8818711426429022	6.5840384580651055	2.2354245576256306
9	82.44252527000407	6.414493903678883	7.852185114687278	3.290795711629766
10-11	40.051055633336304	28.434952279908153	19.489321314896674	12.024670771858867
12-13	30.075106489711096	26.027040899856267	27.640102938597487	16.257749671835153
14-15	16.488207914060137	31.412090565923407	34.46083375136845	17.638867768648005
16-17	21.93877402390986	25.521232979270703	32.19339382170322	20.34659917511622
18-19	24.004304450748474	27.44498104421967	30.215694465564685	18.335020039467175
20-21	27.08547084736737	26.448309594182582	29.30143565201534	17.16478390643471
22-23	22.46136161173365	24.490769143823304	30.841144909322793	22.20672433512025
24-25	19.32446691055447	26.63375987480504	34.58641907335453	19.455354141285962
26-27	18.419384477526023	33.5143847775791	28.960190822104646	19.106039922790224
28-29	17.40645772485191	27.936893302873578	35.35708938027678	19.29955959199773
30-31	22.785651986205714	28.493098487907275	30.18073682548505	18.540512700401955
32-33	25.96577763561671	27.600280360273437	28.033988148194762	18.399953855915093
34-35	20.516668094103636	28.173090424344966	30.005103815451626	21.30513766609977
36-37	22.880387190821523	29.161896405364136	29.7086338962096	18.249082507604744
38-39	19.769011566900474	30.820549033042543	28.51803493783075	20.89240446222623
40-41	21.418808259092046	27.005534377051944	28.475765324701126	23.099892039154888
42-43	21.044528459306104	26.23844722823785	28.544049248323343	24.172975064132704
44-45	19.31881542540826	31.23150522354537	28.345664640871426	21.104014710174944
46-47	21.001355773807756	30.24194182699114	27.789342930370793	20.967359468830313
48-49	20.895638043933598	26.880881258801313	31.439211868351858	20.78426882891323
50-51	19.398227531122494	30.840504019254666	28.805561527485736	20.9557069221371
52-53	22.154637451401598	26.55018198364798	29.667675194582966	21.62750537036746
54-55	21.83276498036837	26.775804418995282	30.725784697060003	20.66564590357634
56-57	23.038832694482462	26.008921189748325	28.445235652364914	22.507010463404303
58-59	23.606107798874714	27.225650984086702	28.441419443322886	20.726821773715702
60-61	24.248075727570452	28.853599151228497	26.521138011015154	20.377187110185897
62-63	23.356626774173062	25.731881892117226	30.448774530795642	20.46271680291407
64-65	20.193397317467227	29.981507408397874	29.814031181049693	20.011064093085203
66-67	19.864008953816878	28.628967182350117	25.731270133415833	25.77575373041717
68-69	18.702308311237193	31.59529773130742	26.103598131630662	23.598795825824723
70-71	19.599962245748713	29.580718064884877	28.807455066323385	22.011864623043028
72-73	22.293448413884477	29.917156219284614	27.454681789341766	20.334713577489143
74-75	21.38326799167775	31.639053044140432	25.33109254860423	21.64658641557759
76-77	25.042837674780916	28.44943056917447	26.461826548346707	20.045905207697906
78-79	19.697843637971687	29.271226424713188	29.55866562026797	21.472264317047152
80-81	17.38239521593043	28.754261190668874	30.389870907261457	23.47347268613924
82-83	19.789432654980327	27.714184004199577	29.844823035686506	22.65156030513359
84-85	18.44032993020707	27.61912835454971	28.72498416710218	25.21555754814104
86	18.55895285554396	27.43335762889319	30.866605723847464	23.14108379171539
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	680.0
1	1045.5
2	3461.5
3	3601.5
4	1768.5
5	2074.0
6	2502.5
7	2703.0
8	2820.5
9	2874.0
10	2870.5
11	2997.5
12	3110.5
13	3191.5
14	3226.0
15	3190.5
16	3199.5
17	3715.0
18	3783.5
19	3546.5
20	4038.0
21	4364.0
22	4773.0
23	5653.0
24	7051.0
25	8522.0
26	11449.0
27	15090.0
28	17242.5
29	18137.0
30	21423.5
31	28024.5
32	32525.5
33	35750.0
34	37910.5
35	42057.5
36	46082.0
37	49604.5
38	53252.0
39	56192.0
40	66840.5
41	75355.0
42	79897.0
43	83758.0
44	83196.5
45	87343.5
46	92699.5
47	94958.5
48	97018.0
49	88995.5
50	80525.0
51	72706.5
52	59156.5
53	51542.0
54	47081.5
55	45339.0
56	42156.0
57	38138.0
58	34878.0
59	27139.5
60	19770.5
61	16878.0
62	15066.5
63	11412.5
64	9568.0
65	7951.0
66	5721.0
67	4228.0
68	3041.0
69	2112.5
70	1151.0
71	811.0
72	704.0
73	541.0
74	313.5
75	130.5
76	135.5
77	104.5
78	41.5
79	26.0
80	25.0
81	21.0
82	17.0
83	13.0
84	10.0
85	9.0
86	8.0
87	5.0
88	2.5
89	2.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1716363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.63347515423413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52691617656963	12.745490184477298
2	7.702488500551382	2.4083332519828824
3	2.2589453822861634	1.0594549952622794
4	1.0429251354338724	0.6521817677012683
5	0.6232473933211125	0.4871761319213399
6	0.41476155919667257	0.38904987183795536
7	0.31470131188997885	0.34439125983058094
8	0.24072657191563013	0.30107143048055673
9	0.20254058856116045	0.2849771933095373
>10	2.4983132615348547	9.632031194052287
>50	1.2070576632520922	13.778870513231642
>100	1.9602496389661277	56.49605477310495
>500	0.005626434095769598	0.5905682582561076
>1k	0.0011252868191539195	0.3280934768089487
>5k	3.750956063846398E-4	0.5022556977423852
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8565	0.4990203121367683	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	2265	0.13196509130061648	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAA	2077	0.12101169740899798	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1652546693211168E-4	0.0	0.0	0.022897254252159945	0.0
2	1.1652546693211168E-4	0.0	0.0	0.068866550956878	0.0
3	1.1652546693211168E-4	0.0	0.0	0.09479346734927285	0.0
4	1.1652546693211168E-4	0.0	0.0	0.1515996324786773	0.0
5	1.1652546693211168E-4	0.0	0.0	0.28001069703786435	0.0
6	1.1652546693211168E-4	0.0	0.0	0.37474590165367117	0.0
7	1.1652546693211168E-4	0.0	0.0	0.4387766457328665	0.0
8	1.1652546693211168E-4	0.0	0.0	0.5716156780354739	0.0
9	1.1652546693211168E-4	0.0	0.0	0.6109430231250615	0.0
10	1.1652546693211168E-4	0.0	0.0	0.7025903028671674	0.0
11	1.1652546693211168E-4	0.0	0.0	0.8625215062314907	0.0
12	1.1652546693211168E-4	0.0	0.0	0.9852228229110043	0.0
13	1.1652546693211168E-4	0.0	0.0	1.0285120338762836	0.0
14	1.1652546693211168E-4	0.0	0.0	1.0462821675834308	0.0
15	1.1652546693211168E-4	0.0	0.0	1.0805989175949378	0.0
16	1.1652546693211168E-4	0.0	0.0	1.1489411039506212	0.0
17	1.1652546693211168E-4	0.0	0.0	1.2347038476126553	0.0
18	1.1652546693211168E-4	0.0	0.0	1.3254189236193044	0.0
19	1.1652546693211168E-4	0.0	0.0	1.389915769566228	0.0
20	1.1652546693211168E-4	0.0	0.0	1.4749793604266697	0.0
21	1.1652546693211168E-4	0.0	0.0	1.568141471238893	0.0
22	1.1652546693211168E-4	0.0	0.0	1.674704010748309	0.0
23	1.1652546693211168E-4	0.0	0.0	1.7791690918529472	0.0
24	1.1652546693211168E-4	0.0	0.0	1.8568915782966657	0.0
25	1.1652546693211168E-4	0.0	0.0	1.9329827082033346	0.0
26	1.1652546693211168E-4	0.0	0.0	2.0056945995689723	0.0
27	1.1652546693211168E-4	0.0	0.0	2.0820187804095056	0.0
28	1.1652546693211168E-4	0.0	0.0	2.1600908432540202	0.0
29	1.1652546693211168E-4	0.0	0.0	2.257680921809664	0.0
30	1.1652546693211168E-4	0.0	0.0	2.371526303002337	0.0
31	1.1652546693211168E-4	0.0	0.0	2.469524220692243	0.0
32	1.1652546693211168E-4	0.0	0.0	2.5649585781096422	0.0
33	1.1652546693211168E-4	0.0	0.0	2.6579459007214674	0.0
34	1.1652546693211168E-4	0.0	0.0	2.7690529334412357	0.0
35	1.1652546693211168E-4	0.0	0.0	2.8907637836518267	0.0
36	1.1652546693211168E-4	0.0	0.0	2.9972680604277766	0.0
37	1.1652546693211168E-4	0.0	0.0	3.111637806221644	0.0
38	1.1652546693211168E-4	0.0	0.0	3.2412141254501523	0.0
39	1.1652546693211168E-4	0.0	0.0	3.448571193855845	0.0
40	1.1652546693211168E-4	0.0	0.0	3.5970246387273557	0.0
41	1.1652546693211168E-4	0.0	0.0	3.733184646837528	0.0
42	1.1652546693211168E-4	0.0	0.0	3.866664569208262	0.0
43	1.1652546693211168E-4	0.0	0.0	3.9956582611021094	0.0
44	1.1652546693211168E-4	0.0	0.0	4.130478226342563	0.0
45	1.1652546693211168E-4	0.0	0.0	4.258423189034021	0.0
46	1.1652546693211168E-4	0.0	0.0	4.405827904703143	0.0
47	1.1652546693211168E-4	0.0	0.0	4.577062078359881	0.0
48	1.1652546693211168E-4	0.0	0.0	4.715028231207501	0.0
49	1.1652546693211168E-4	0.0	0.0	4.8644721425479345	0.0
50	1.1652546693211168E-4	0.0	0.0	5.015372622225019	0.0
51	1.1652546693211168E-4	0.0	0.0	5.175828190190536	0.0
52	1.1652546693211168E-4	0.0	0.0	5.364366395686694	0.0
53	1.1652546693211168E-4	0.0	0.0	5.5237732344498225	0.0
54	1.1652546693211168E-4	0.0	0.0	5.687491515489439	0.0
55	1.1652546693211168E-4	0.0	0.0	5.865542428961706	0.0
56	1.1652546693211168E-4	0.0	0.0	6.04703084370847	0.0
57	1.1652546693211168E-4	0.0	0.0	6.216284084427362	0.0
58	1.1652546693211168E-4	0.0	0.0	6.38396423134267	0.0
59	1.1652546693211168E-4	0.0	0.0	6.565044806955172	0.0
60	1.1652546693211168E-4	0.0	0.0	6.763604202607491	5.826273346605584E-5
61	1.1652546693211168E-4	0.0	0.0	6.965659362267772	5.826273346605584E-5
62	1.1652546693211168E-4	0.0	0.0	7.19317533645272	5.826273346605584E-5
63	1.1652546693211168E-4	0.0	0.0	7.432227331863947	5.826273346605584E-5
64	1.1652546693211168E-4	0.0	0.0	7.639700925736572	5.826273346605584E-5
65	1.1652546693211168E-4	0.0	0.0	7.842047399064184	5.826273346605584E-5
66	1.747882003981675E-4	0.0	0.0	8.03856759904519	5.826273346605584E-5
67	1.747882003981675E-4	0.0	0.0	8.227513643675609	5.826273346605584E-5
68	2.3305093386422336E-4	0.0	0.0	8.469420513026673	5.826273346605584E-5
69	2.3305093386422336E-4	0.0	0.0	8.790040335290378	5.826273346605584E-5
70	3.49576400796335E-4	0.0	0.0	9.01557537653748	5.826273346605584E-5
71	4.078391342623909E-4	0.0	0.0	9.232545795965073	5.826273346605584E-5
72	4.078391342623909E-4	0.0	0.0	9.442932526511001	5.826273346605584E-5
73	4.078391342623909E-4	0.0	0.0	9.66363176088042	5.826273346605584E-5
74	4.078391342623909E-4	0.0	0.0	9.903790748227502	5.826273346605584E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	130	0.0	73.84615	1
ACGGGTA	50	3.6379788E-12	72.0	5
ATCGTAG	45	1.1823431E-10	71.11111	1
TAACGGG	485	0.0	69.27835	3
TACGGGA	775	0.0	69.16129	4
CGTTTTT	4485	0.0	68.94091	1
ATAGCGG	385	0.0	68.571434	2
TAGCGGG	1230	0.0	67.64227	3
ACGGGAT	1065	0.0	66.10329	5
TAGCACG	80	0.0	65.0	1
CGGGTAG	105	0.0	64.76191	6
ACGGGAC	540	0.0	64.44444	5
ATAGGGA	2190	0.0	63.744293	4
CGGGATC	685	0.0	63.649635	6
CGTTAGG	235	0.0	62.978725	2
AGGGATT	3940	0.0	62.33503	6
GCGAGGG	1680	0.0	61.904766	3
CGAGGGA	1620	0.0	61.7284	4
TGCACGG	365	0.0	61.369865	2
CGGGATA	555	0.0	61.261265	6
>>END_MODULE