FastQCFastQC Report
Thu 26 May 2016
SRR1547759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547759_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1409080
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85580.607346637522355No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20710.1469753314219207No Hit
TATAGAGGGACTCTAGGCAGTATTTTTATAGATTATGATTATGTGGCAAT15880.11269764669145825No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT150.001309852680.000019
CAATACG150.001309852680.000011
TGCGTAG1200.080.000011
CGTTAGG1400.077.142852
TTCGTAG750.074.6666641
ACGTAGG2750.072.727272
TAGGGCG1750.070.857155
CGAAAGG3900.070.7692262
TAGCGGG7400.070.270273
ACGTTAG1200.070.000011
CGTTTTT47550.069.4006351
TAGGGTC3750.069.3333365
TACGTAG1850.069.189191
ATTAGCG600.066.666671
AGCGGGA9550.065.340314
GTAAGGG19250.064.831173
TAGGGAC13500.064.592595
GGCGATT3100.064.516138
AAGTACT44350.064.0360780
GCCGATC251.2356229E-464.000019
Produced by FastQC (version 0.11.5)