##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547759_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1409080
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.812608226644336	31.0	31.0	33.0	30.0	34.0
2	32.264999148380504	33.0	31.0	34.0	31.0	34.0
3	32.4214345530417	34.0	31.0	34.0	31.0	34.0
4	36.010500468390724	37.0	35.0	37.0	35.0	37.0
5	35.95589746501263	37.0	35.0	37.0	35.0	37.0
6	36.020965452635764	37.0	35.0	37.0	35.0	37.0
7	36.25421054872683	37.0	35.0	37.0	35.0	37.0
8	36.221396939847274	37.0	36.0	37.0	35.0	37.0
9	38.14451131234564	39.0	39.0	39.0	37.0	39.0
10-11	37.57447093138786	39.0	37.5	39.0	35.0	39.0
12-13	36.389513370426094	38.0	35.0	39.0	33.0	39.0
14-15	37.292903525704716	39.0	35.0	41.0	33.0	41.0
16-17	37.5604628551963	39.0	35.0	41.0	33.5	41.0
18-19	37.50355196298294	39.0	35.0	41.0	33.5	41.0
20-21	37.26848262696227	39.0	35.0	41.0	33.5	41.0
22-23	37.087935745309	38.0	35.0	41.0	33.0	41.0
24-25	37.050470519771764	38.0	35.0	41.0	33.0	41.0
26-27	37.0301512334289	38.0	35.0	41.0	33.0	41.0
28-29	37.06473408181225	38.0	35.0	41.0	33.0	41.0
30-31	36.89370333834843	38.0	35.0	41.0	33.0	41.0
32-33	36.54477354018225	38.0	35.0	41.0	32.0	41.0
34-35	36.269327504471	38.0	35.0	41.0	31.0	41.0
36-37	36.09794759701366	38.0	35.0	41.0	31.0	41.0
38-39	36.029237871519	38.0	35.0	40.5	31.0	41.0
40-41	35.87949655094104	38.0	35.0	40.0	30.0	41.0
42-43	35.860943665370314	38.0	35.0	40.0	30.0	41.0
44-45	35.75259743946405	37.0	35.0	40.0	30.0	41.0
46-47	35.69193267947881	37.0	35.0	40.0	30.0	41.0
48-49	35.63496039969341	37.0	35.0	40.0	30.0	41.0
50-51	35.45808044965509	36.0	35.0	40.0	30.0	41.0
52-53	35.25783312515968	36.0	34.5	40.0	29.0	41.0
54-55	35.087654710875185	36.0	34.0	40.0	29.0	41.0
56-57	34.77655988304426	35.5	34.0	39.5	28.0	41.0
58-59	34.46624038379652	35.0	34.0	39.0	27.0	41.0
60-61	34.16201918982598	35.0	34.0	39.0	26.5	40.5
62-63	33.723084210974534	35.0	33.5	38.0	26.0	40.0
64-65	33.48687015641411	35.0	33.0	37.5	26.0	40.0
66-67	33.279895392738524	35.0	33.0	37.0	25.0	40.0
68-69	32.9651751497431	35.0	33.0	36.5	25.0	39.0
70-71	32.789247594174924	35.0	33.0	36.0	25.0	39.0
72-73	32.56799613932495	35.0	33.0	36.0	24.5	39.0
74-75	32.2902014080109	35.0	33.0	35.0	24.0	37.5
76-77	30.252905087007125	33.0	29.5	34.5	19.5	35.5
78-79	31.27479028869901	34.0	30.5	35.0	21.0	36.0
80-81	31.68133392000454	35.0	32.0	35.0	23.0	36.0
82-83	31.603043120333837	35.0	32.0	35.0	21.5	36.0
84-85	31.447018622079653	35.0	32.0	35.0	20.0	36.0
86	31.045763193005364	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	3.0
10	6.0
11	8.0
12	9.0
13	54.0
14	189.0
15	486.0
16	1231.0
17	2669.0
18	5433.0
19	9195.0
20	11271.0
21	11694.0
22	10585.0
23	9453.0
24	9814.0
25	11313.0
26	13441.0
27	16126.0
28	20180.0
29	25150.0
30	31868.0
31	40467.0
32	51220.0
33	72334.0
34	206273.0
35	175296.0
36	110993.0
37	195458.0
38	251053.0
39	115501.0
40	300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.855395009509753	24.253697447980244	29.59803559769495	14.292871944815055
2	30.3559059812076	25.083316774065345	31.32909416072899	13.23168308399807
3	28.469213955204815	23.98891475288841	33.38163908365742	14.160232208249354
4	26.356345984614077	27.769466602322083	32.17006841343288	13.704118999630966
5	23.064268884662333	32.01805433332387	31.068427626536465	13.849249155477333
6	22.299443608595677	41.03315638572686	26.707993868339624	9.959406137337838
7	89.39811792091294	3.2834899366962844	5.976594657507025	1.341797484883754
8	90.75893490788316	2.322721208164192	5.649501802594601	1.2688420813580492
9	87.4401027620859	3.9522951145428227	6.609276975047548	1.9983251483237292
10-11	46.76366849291737	23.548130695205383	17.714253271638235	11.973947540239022
12-13	32.930422687143384	27.517103358219547	24.198519601442076	15.353954353194993
14-15	15.06018111107957	33.369893831436116	36.45797257785222	15.111952479632102
16-17	20.213259715559087	22.864493144463054	35.074871547392625	21.847375592585234
18-19	24.67858460839697	26.40183665938059	31.076127686149828	17.843451046072616
20-21	27.620326738013457	26.91415675476197	28.540395151446337	16.925121355778238
22-23	21.46166292900332	24.585935504016806	30.15538507394896	23.797016493030913
24-25	18.60465693927953	25.474032702188666	37.54307775286002	18.378232605671784
26-27	16.676164589661337	36.26983563743719	28.581201918982597	18.47279785391887
28-29	14.84681494308343	28.466339739404432	37.88298038436427	18.80386493314787
30-31	23.35435177562665	29.731775342776846	29.47078945127317	17.44308343032333
32-33	27.952032531864763	27.127522922758114	27.923680699463482	16.996763845913645
34-35	19.08873165469668	28.642199165412897	29.74415930962046	22.524909870269962
36-37	24.560564339852952	28.785555113975075	29.712684872399013	16.941195673772956
38-39	19.190109858915037	32.42150906974764	27.589774888579782	20.798606182757545
40-41	20.586659380588753	26.30627785505436	28.19761830414171	24.909444460215177
42-43	20.614337014222045	24.493712209384846	28.562679194935704	26.3292715814574
44-45	18.164298691344708	32.170991001220656	28.37060351434979	21.29410679308485
46-47	20.869255116813807	30.649359866011867	27.012057512703326	21.469327504471003
48-49	20.723521730490816	25.493797371334487	32.51312913390297	21.269551764271725
50-51	19.156683793680983	32.205410622533854	28.0863400232776	20.551565560507566
52-53	22.54495841258126	25.79789649984387	30.8590356828569	20.79810940471797
54-55	23.845523320180543	26.12254094870412	30.613449910579956	19.418485820535388
56-57	23.610902148919862	23.40555539784824	29.736565702444146	23.24697675078775
58-59	25.588256167144518	26.338249070315385	28.0506074885741	20.022887273965992
60-61	26.39083657421864	28.054368808016577	26.294390666250315	19.260403951514462
62-63	24.557299798450053	24.591861356345984	30.997281914440627	19.853556930763336
64-65	18.65490248956766	29.886663638686233	31.761397507593607	19.697036364152495
66-67	17.827696085389046	28.183105288557076	25.380673914894825	28.608524711159056
68-69	17.16864904760553	32.457312572742495	24.620709966786837	25.753328412865134
70-71	18.577937377579698	30.434077554148807	29.077447696369262	21.910537371902233
72-73	23.122143526272463	29.322217333295487	26.64557725608198	20.91006188435007
74-75	22.54495841258126	32.41434127231953	23.275754392937237	21.764945922161978
76-77	28.12526613109263	27.51156783149289	25.050671360036336	19.312494677378147
78-79	19.015740767025292	28.067391489482503	31.274342123939025	21.642525619553183
80-81	15.971981718568143	27.76936014988503	31.92533426065234	24.333323870894485
82-83	17.98989411530928	25.88291651290204	32.95142930138814	23.175760070400546
84-85	15.843990405087007	25.052658472194622	30.82947738950237	28.273873733216
86	17.75009225877878	25.647727595310414	31.914724501092913	24.687455644817895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1155.0
1	1123.0
2	3338.5
3	3464.0
4	1497.5
5	1783.0
6	2200.5
7	2488.0
8	2628.0
9	2748.5
10	2646.5
11	2553.0
12	2573.5
13	2644.0
14	2683.0
15	2755.0
16	2977.5
17	3388.0
18	3593.5
19	3472.0
20	3296.0
21	3187.0
22	4021.0
23	4678.0
24	4907.0
25	7077.0
26	9485.5
27	10518.5
28	11429.5
29	11952.0
30	14848.0
31	19851.5
32	21408.5
33	23354.0
34	28400.5
35	31778.5
36	32606.0
37	32283.0
38	33429.0
39	40352.0
40	51086.0
41	63386.5
42	73719.0
43	77031.0
44	76031.5
45	78135.5
46	86490.0
47	92741.0
48	90889.0
49	79100.5
50	70164.0
51	60740.0
52	47081.5
53	41539.5
54	38480.0
55	36779.5
56	35108.5
57	33386.0
58	30816.0
59	23095.5
60	15558.5
61	11453.0
62	9342.5
63	7498.5
64	6046.0
65	4705.5
66	2761.0
67	1764.5
68	1313.0
69	1009.0
70	650.0
71	536.0
72	331.5
73	96.0
74	50.5
75	36.0
76	33.5
77	68.5
78	62.0
79	18.0
80	12.0
81	7.0
82	8.0
83	6.5
84	4.5
85	3.0
86	2.0
87	1.5
88	1.0
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1409080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.014995507638274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40191971526218	12.072344633301547
2	8.649035887208163	2.5973046998366556
3	2.627482289955351	1.1835490434023617
4	1.2112449280903386	0.7274734861570431
5	0.6947029245743936	0.5215480645813846
6	0.5122550064140733	0.46149039720435153
7	0.3518035706573081	0.36976303230934093
8	0.2663458671072099	0.3199345598474222
9	0.21880389905634115	0.29568056052462305
>10	2.136075920241242	7.551850680674306
>50	0.9412459764626369	10.328736244047423
>100	1.972904862695905	61.17753654564497
>500	0.014275722595484118	1.4285707044419493
>1k	0.0014275722595484118	0.35274850294237786
>5k	4.7585741984947056E-4	0.6114688450842353
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8558	0.607346637522355	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2071	0.1469753314219207	No Hit
TATAGAGGGACTCTAGGCAGTATTTTTATAGATTATGATTATGTGGCAAT	1588	0.11269764669145825	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04215516507224572	0.0
2	0.0	0.0	0.0	0.1454140290118375	0.0
3	0.0	0.0	0.0	0.20431771084679365	0.0
4	0.0	0.0	0.0	0.30686689187271127	0.0
5	0.0	0.0	0.0	0.5280750560649502	0.0
6	0.0	0.0	0.0	0.6953473188179521	0.0
7	0.0	0.0	0.0	0.8206773213728106	0.0
8	0.0	0.0	0.0	1.0490532829931587	0.0
9	0.0	0.0	0.0	1.1101569818605048	0.0
10	0.0	0.0	0.0	1.2466999744514151	0.0
11	0.0	0.0	0.0	1.4750759360717631	0.0
12	0.0	0.0	0.0	1.6378062281772503	0.0
13	0.0	0.0	0.0	1.6992647685014335	0.0
14	0.0	0.0	0.0	1.724174638771397	0.0
15	0.0	0.0	0.0	1.7649814063076616	0.0
16	0.0	0.0	0.0	1.8611434411105119	0.0
17	0.0	0.0	0.0	1.9727055951400914	0.0
18	0.0	0.0	0.0	2.106196951202203	0.0
19	0.0	0.0	0.0	2.190933091094899	0.0
20	0.0	0.0	0.0	2.285178982030829	0.0
21	0.0	0.0	0.0	2.4206574502512277	0.0
22	0.0	0.0	0.0	2.561742413489653	0.0
23	0.0	0.0	0.0	2.7038209328072216	0.0
24	0.0	0.0	0.0	2.81871859653107	0.0
25	0.0	0.0	0.0	2.9147386947511853	0.0
26	0.0	0.0	0.0	3.0075652198597664	0.0
27	0.0	0.0	0.0	3.106707922900048	0.0
28	0.0	0.0	0.0	3.211670025832458	0.0
29	0.0	0.0	0.0	3.343742016067221	0.0
30	0.0	0.0	0.0	3.505194878928095	0.0
31	0.0	0.0	0.0	3.640176569108922	0.0
32	0.0	0.0	0.0	3.7599000766457547	0.0
33	0.0	0.0	0.0	3.884733301161041	0.0
34	0.0	0.0	0.0	4.016805291395804	0.0
35	0.0	0.0	0.0	4.185354983393419	0.0
36	0.0	0.0	0.0	4.321259261362023	0.0
37	0.0	0.0	0.0	4.474621739007012	0.0
38	0.0	0.0	0.0	4.623371277713118	0.0
39	0.0	0.0	0.0	4.855863400232776	0.0
40	0.0	0.0	0.0	5.01951628012604	0.0
41	0.0	0.0	0.0	5.185369177051693	0.0
42	0.0	0.0	0.0	5.350725295937775	0.0
43	0.0	0.0	0.0	5.501107105345332	0.0
44	0.0	0.0	0.0	5.663766429159452	0.0
45	0.0	0.0	0.0	5.823019274987935	0.0
46	0.0	0.0	0.0	5.993627047435206	0.0
47	0.0	0.0	0.0	6.164944502796151	0.0
48	0.0	0.0	0.0	6.33392000454197	0.0
49	0.0	0.0	0.0	6.52077951571238	1.4193658273483408E-4
50	0.0	0.0	0.0	6.716155221846879	1.4193658273483408E-4
51	0.0	0.0	0.0	6.897975984330201	1.4193658273483408E-4
52	0.0	0.0	0.0	7.117835750986459	1.4193658273483408E-4
53	0.0	0.0	0.0	7.314205013200103	1.4193658273483408E-4
54	0.0	0.0	0.0	7.525619553183637	1.4193658273483408E-4
55	0.0	0.0	0.0	7.742995429642036	1.4193658273483408E-4
56	0.0	0.0	0.0	7.965764896244358	1.4193658273483408E-4
57	0.0	0.0	0.0	8.16738581201919	1.4193658273483408E-4
58	0.0	0.0	0.0	8.36162602549181	1.4193658273483408E-4
59	0.0	0.0	0.0	8.572543787435773	1.4193658273483408E-4
60	0.0	0.0	0.0	8.813268231754053	1.4193658273483408E-4
61	0.0	0.0	0.0	9.051366849291737	1.4193658273483408E-4
62	0.0	0.0	0.0	9.322252817441168	1.4193658273483408E-4
63	0.0	0.0	0.0	9.621809975303034	1.4193658273483408E-4
64	7.096829136741704E-5	0.0	0.0	9.895250801941692	1.4193658273483408E-4
65	7.096829136741704E-5	0.0	0.0	10.131859085360661	1.4193658273483408E-4
66	7.096829136741704E-5	0.0	0.0	10.363215715218441	1.4193658273483408E-4
67	7.096829136741704E-5	0.0	0.0	10.587404547648111	1.4193658273483408E-4
68	7.096829136741704E-5	0.0	0.0	10.85396145002413	1.4193658273483408E-4
69	1.4193658273483408E-4	0.0	0.0	11.21497714821018	1.4193658273483408E-4
70	1.4193658273483408E-4	0.0	0.0	11.4532177023306	1.4193658273483408E-4
71	1.4193658273483408E-4	0.0	0.0	11.704942231810827	1.4193658273483408E-4
72	2.1290487410225112E-4	0.0	0.0	11.94971186873705	1.4193658273483408E-4
73	2.1290487410225112E-4	0.0	0.0	12.219107502767763	1.4193658273483408E-4
74	2.1290487410225112E-4	0.0	0.0	12.500070968291368	1.4193658273483408E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCGT	15	0.0013098526	80.00001	9
CAATACG	15	0.0013098526	80.00001	1
TGCGTAG	120	0.0	80.00001	1
CGTTAGG	140	0.0	77.14285	2
TTCGTAG	75	0.0	74.666664	1
ACGTAGG	275	0.0	72.72727	2
TAGGGCG	175	0.0	70.85715	5
CGAAAGG	390	0.0	70.769226	2
TAGCGGG	740	0.0	70.27027	3
ACGTTAG	120	0.0	70.00001	1
CGTTTTT	4755	0.0	69.400635	1
TAGGGTC	375	0.0	69.333336	5
TACGTAG	185	0.0	69.18919	1
ATTAGCG	60	0.0	66.66667	1
AGCGGGA	955	0.0	65.34031	4
GTAAGGG	1925	0.0	64.83117	3
TAGGGAC	1350	0.0	64.59259	5
GGCGATT	310	0.0	64.51613	8
AAGTACT	4435	0.0	64.03607	80
GCCGATC	25	1.2356229E-4	64.00001	9
>>END_MODULE