FastQCFastQC Report
Thu 26 May 2016
SRR1547758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547758_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences924185
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64390.696721976660517No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26230.2838176339152875No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18250.1974712855110178No Hit
GTCTATGGGGGCCATATACTGCATTCAACTCTGATCCCCTTCTACCTACT11490.1243257572888545No Hit
GTTTTGGTTGGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCA10910.11804995753014819No Hit
GGGCAAGGGAAATACGATGAACTTAAAAAATCAGAAAAGGGAAGTAAACA10460.113180802544945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAC204.0904753E-580.08
GGTCGGT204.0904753E-580.09
ATTACGG404.1836756E-1180.02
TAGCGAG451.8189894E-1280.01
GCGTTCG251.2846049E-680.080
CCGCTAG404.1836756E-1180.01
TAGCCCG404.1836756E-1180.01
CGTTAAG204.0904753E-580.08
TACGACT204.0904753E-580.06
ACTACGG500.080.02
GTCGTAA150.001309709480.08
TATTACG404.1836756E-1180.01
GAACGAT204.0904753E-580.09
TGTAGCG700.080.01
CGCGTGG251.2846049E-680.02
CGTCAGG600.080.02
ATCGCTG451.8189894E-1280.01
CGGGACT1500.077.333336
GGAGCGT1000.076.08
TACGGGA2550.075.294124
Produced by FastQC (version 0.11.5)