##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547758_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	924185
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.814752457570723	31.0	31.0	33.0	30.0	34.0
2	32.23751629814377	33.0	31.0	34.0	31.0	34.0
3	32.384443590839496	34.0	31.0	34.0	31.0	34.0
4	35.990348252784884	37.0	35.0	37.0	35.0	37.0
5	35.927053566115006	37.0	35.0	37.0	35.0	37.0
6	35.97430709219474	37.0	35.0	37.0	35.0	37.0
7	36.23812548353414	37.0	35.0	37.0	35.0	37.0
8	36.20345493597061	37.0	36.0	37.0	35.0	37.0
9	38.136245448692634	39.0	39.0	39.0	37.0	39.0
10-11	37.54265271563594	39.0	37.5	39.0	35.0	39.0
12-13	36.04131802615277	37.0	35.0	39.0	32.5	39.0
14-15	36.82540400460947	38.0	35.0	41.0	32.5	41.0
16-17	37.20144235191006	38.0	35.0	41.0	33.0	41.0
18-19	37.10190816773698	37.5	35.0	41.0	33.0	41.0
20-21	36.807216087688076	37.0	35.0	41.0	33.0	41.0
22-23	36.58685544560883	36.5	35.0	41.0	33.0	41.0
24-25	36.52300621628787	36.0	35.0	40.5	33.0	41.0
26-27	36.475729426467645	36.0	35.0	40.0	33.0	41.0
28-29	36.54241304500722	36.0	35.0	40.0	33.0	41.0
30-31	36.3903433836299	36.0	35.0	40.0	32.5	41.0
32-33	35.915565065436034	36.0	35.0	40.0	31.5	41.0
34-35	35.59390706406185	35.5	35.0	40.0	30.0	41.0
36-37	35.32214112975216	35.0	35.0	40.0	30.0	41.0
38-39	35.29881246720083	35.5	35.0	40.0	30.0	41.0
40-41	35.17803740593063	36.0	35.0	40.0	29.0	41.0
42-43	35.13032293317896	35.5	35.0	40.0	29.0	41.0
44-45	34.96528400698995	35.0	34.0	40.0	29.0	41.0
46-47	34.882670136390445	35.0	34.0	40.0	28.0	41.0
48-49	34.83751521610933	35.5	34.5	40.0	28.0	41.0
50-51	34.63081363579803	35.0	34.0	40.0	27.5	41.0
52-53	34.45608292711957	35.0	34.0	39.5	27.0	41.0
54-55	34.294720212944384	35.0	34.0	39.0	26.5	41.0
56-57	33.98150153919399	35.0	34.0	39.0	25.5	41.0
58-59	33.684841779513846	35.0	34.0	38.5	25.0	40.5
60-61	33.405060134064065	35.0	33.0	37.5	25.0	40.0
62-63	32.94970325205451	35.0	32.5	37.0	23.5	40.0
64-65	32.7221395067005	35.0	32.5	36.5	23.0	40.0
66-67	32.51988941608011	35.0	33.0	36.0	22.5	39.0
68-69	32.29701033883909	35.0	33.0	36.0	21.0	39.0
70-71	32.101346050844796	35.0	33.0	35.0	20.0	38.5
72-73	31.87785832923062	35.0	32.5	35.0	20.0	37.5
74-75	31.615486076921826	35.0	32.0	35.0	19.0	37.0
76-77	29.58431861586154	32.5	28.5	34.5	16.0	35.5
78-79	30.548423205310627	34.0	30.5	35.0	17.0	36.0
80-81	31.076504163127513	34.5	31.5	35.0	18.0	36.0
82-83	31.003990543018986	34.5	31.5	35.0	18.0	36.0
84-85	30.86505623874008	34.5	31.5	35.0	17.5	35.5
86	30.496674367145108	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	4.0
11	5.0
12	19.0
13	44.0
14	160.0
15	549.0
16	1384.0
17	3060.0
18	5953.0
19	8616.0
20	9894.0
21	10045.0
22	8271.0
23	6980.0
24	7342.0
25	8303.0
26	9879.0
27	12183.0
28	14861.0
29	18456.0
30	22896.0
31	28171.0
32	35144.0
33	50352.0
34	157608.0
35	136959.0
36	61988.0
37	105643.0
38	137305.0
39	61864.0
40	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.151014136779974	23.940336620914643	29.1375644486764	15.771084793628981
2	28.604770689851055	26.547173996548313	32.58081444732386	12.267240866276774
3	26.80902633130813	24.12395786557886	34.459442644059365	14.607573159053652
4	25.28465621060718	26.993621406969382	32.89601108003268	14.825711302390754
5	23.251513495674565	31.878357688125213	32.328375812202104	12.541753003998119
6	21.244988827994398	39.045970233232524	28.805163468353197	10.903877470419882
7	87.46820171286052	3.5053587755698263	7.751045515778768	1.275393995790886
8	88.63192975432408	2.9134859362573513	6.821686134269654	1.632898175148915
9	86.25253601822146	4.216688217185953	7.6743292739007885	1.8564464906917988
10-11	51.722707033764884	21.46550744710204	16.52493818878255	10.286847330350525
12-13	34.288751710966956	31.65129276064857	21.61125748632579	12.448698042058679
14-15	11.219831527237512	37.39094445376197	38.79969919442536	12.589524824575166
16-17	14.185201014948307	21.196892397084998	41.05217029058035	23.565736297386348
18-19	22.335679544679905	25.182890871416436	33.2309548412926	19.250474742611058
20-21	25.178184021597406	27.257637810611513	30.38298609044726	17.18119207734382
22-23	18.746841811974875	25.029133777328134	30.02256041809811	26.20146399259888
24-25	15.075120241077272	24.12720396890233	42.61911846654079	18.17855732347961
26-27	12.944864935050882	39.397956036940656	30.56325302834389	17.09392599966457
28-29	11.623646780677028	29.364575274430987	41.94836531646802	17.06341262842396
30-31	21.327818564464906	31.494181359792684	31.32922520923841	15.848774866504002
32-33	28.05082315770111	30.226253401645774	26.98042058678728	14.742502853865838
34-35	17.363190270346305	30.863463484042697	29.06679939622478	22.706546849386218
36-37	23.821475137553627	29.02773795289904	31.845463841114064	15.30532306843327
38-39	16.837970752609056	32.95022100553461	29.75848991273392	20.453318329122418
40-41	19.41045353473601	25.521838160108633	29.232621174331978	25.835087130823375
42-43	21.25548456207361	23.59873834784161	29.13096403858535	26.01481305149943
44-45	16.74285992523142	35.93788040273322	27.318502247926553	20.00075742410881
46-47	21.024957124385267	33.779275794348536	25.980783068325064	19.214984012941134
48-49	21.775943128269773	25.952866579743233	33.07984873158512	19.191341560401867
50-51	18.27420916807782	34.34404367091005	27.501420170204018	19.88032699080812
52-53	23.382385561332416	26.66289758002997	30.425077230208235	19.529639628429372
54-55	24.4060442443883	26.777755535958708	31.327277547244332	17.488922672408663
56-57	25.063975286333363	22.194257643220784	29.54998187592311	23.19178519452274
58-59	26.003884503643746	25.486726142493115	28.36261138192029	20.146777971942846
60-61	25.814744883329638	29.105482127496117	26.688000779064797	18.391772210109448
62-63	23.63098297418807	24.617095062135828	34.04129043427452	17.71063152940158
64-65	16.91603953753848	31.656432424244063	33.15483371835726	18.2726943198602
66-67	14.68477631643015	28.762152599317236	24.56710507095441	31.985966013298206
68-69	15.016690381254834	35.69025682087461	21.781082791865263	27.511970006005292
70-71	17.700893219431173	30.545669968675103	28.84920227010826	22.904234541785463
72-73	23.987513322549056	30.136174034419515	26.01951990131846	19.85679274171297
74-75	22.57708142850187	35.05613053663498	21.424390138338104	20.942397896525048
76-77	32.050996283211695	28.066025741599354	23.199413537332894	16.683564437856056
78-79	18.012248629873888	30.19503670801842	31.607632670947915	20.185081991159777
80-81	13.18935061703014	29.72262047100959	34.84064337767871	22.247385534281555
82-83	17.709008477739847	23.853178746679507	36.693194544382344	21.7446182311983
84-85	14.287182761027283	24.67417237890682	33.147313578991216	27.891331281074677
86	16.683672641300173	24.133587972105154	34.73384657833659	24.448892808258087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2068.0
1	1721.0
2	2823.0
3	2762.0
4	1382.5
5	1869.5
6	2496.0
7	2766.0
8	2652.5
9	2637.0
10	2538.0
11	2450.0
12	2478.5
13	2477.5
14	2557.0
15	2614.5
16	2632.5
17	2764.0
18	2946.0
19	3037.5
20	3112.5
21	3107.0
22	3727.5
23	3931.0
24	4122.5
25	5093.0
26	5771.5
27	7175.0
28	8892.5
29	9523.0
30	11993.0
31	14931.0
32	15390.5
33	15834.5
34	15533.5
35	17122.5
36	19465.0
37	19035.5
38	20629.5
39	25768.0
40	34907.0
41	46218.0
42	55090.0
43	58675.0
44	62435.5
45	64033.5
46	65986.5
47	70745.5
48	66976.0
49	52115.5
50	41668.0
51	34029.0
52	22981.5
53	18643.0
54	16402.0
55	15642.0
56	16079.5
57	15966.0
58	13734.5
59	9152.5
60	5492.0
61	3921.5
62	3149.5
63	2052.5
64	1467.0
65	1343.0
66	821.5
67	283.0
68	127.5
69	142.5
70	114.5
71	57.0
72	36.0
73	16.5
74	21.0
75	43.0
76	34.0
77	7.5
78	6.5
79	4.0
80	5.0
81	6.5
82	6.0
83	3.5
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	924185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.185059554766408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41195765329806	13.176573830887502
2	8.52212573379706	2.758622250694256
3	2.475075439149976	1.2017773015554596
4	1.1615150276134272	0.7519675958271788
5	0.6894461110167476	0.5579363183304077
6	0.45251129357310554	0.439435334141106
7	0.3418998308694748	0.38735683870709087
8	0.26295218665375597	0.34047174408376746
9	0.20774450694025287	0.3026121495303219
>10	1.9150826346003624	6.759075623533454
>50	0.7165517581228958	8.632798301799175
>100	1.8229722026541213	61.52383745499493
>500	0.016132497368620543	1.6255988938309172
>1k	0.003360936951795947	0.8414122503606153
>5k	6.721873903591894E-4	0.7005241117238171
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6439	0.696721976660517	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2623	0.2838176339152875	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1825	0.1974712855110178	No Hit
GTCTATGGGGGCCATATACTGCATTCAACTCTGATCCCCTTCTACCTACT	1149	0.1243257572888545	No Hit
GTTTTGGTTGGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCA	1091	0.11804995753014819	No Hit
GGGCAAGGGAAATACGATGAACTTAAAAAATCAGAAAAGGGAAGTAAACA	1046	0.113180802544945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07314552822216332	0.0
2	0.0	0.0	0.0	0.23977883216022766	0.0
3	0.0	0.0	0.0	0.32958769077619743	0.0
4	0.0	0.0	0.0	0.49795224982011177	0.0
5	0.0	0.0	0.0	0.7973511796880495	0.0
6	0.0	0.0	0.0	1.061475786774293	0.0
7	0.0	0.0	0.0	1.2098227086568165	0.0
8	0.0	0.0	0.0	1.4854168808193164	0.0
9	0.0	0.0	0.0	1.5761995704323268	0.0
10	0.0	0.0	0.0	1.7537614222260696	0.0
11	0.0	0.0	0.0	2.089624912760973	0.0
12	0.0	0.0	0.0	2.3020282735599475	0.0
13	0.0	0.0	0.0	2.3813413980967013	0.0
14	0.0	0.0	0.0	2.4132614141108113	0.0
15	0.0	0.0	0.0	2.468012356833318	0.0
16	0.0	0.0	0.0	2.6015354068720007	0.0
17	0.0	0.0	0.0	2.747069039207518	0.0
18	0.0	0.0	0.0	2.9191125153513635	0.0
19	0.0	0.0	0.0	3.0219057872612085	0.0
20	0.0	0.0	0.0	3.1264303142769037	0.0
21	0.0	0.0	0.0	3.297067145647246	0.0
22	0.0	0.0	0.0	3.4590477014883385	0.0
23	0.0	0.0	0.0	3.636068536061503	0.0
24	0.0	0.0	0.0	3.7473016766123664	0.0
25	0.0	0.0	0.0	3.858102003386768	0.0
26	0.0	0.0	0.0	3.9648988027288907	0.0
27	0.0	0.0	0.0	4.061091664547683	0.0
28	0.0	0.0	0.0	4.164858767454568	0.0
29	0.0	0.0	0.0	4.291348593625735	0.0
30	0.0	0.0	0.0	4.445322094602271	0.0
31	0.0	0.0	0.0	4.598213561137651	0.0
32	0.0	0.0	0.0	4.731520204288103	0.0
33	0.0	0.0	0.0	4.846432261938897	0.0
34	0.0	0.0	0.0	4.984499856630436	0.0
35	0.0	0.0	0.0	5.154595670780201	0.0
36	0.0	0.0	0.0	5.3068379166508866	0.0
37	0.0	0.0	0.0	5.450423886992323	0.0
38	0.0	0.0	0.0	5.59249500911614	0.0
39	0.0	0.0	0.0	5.742140372328051	0.0
40	0.0	0.0	0.0	5.900442011069212	0.0
41	0.0	0.0	0.0	6.065668670233774	0.0
42	0.0	0.0	0.0	6.239659808371701	0.0
43	0.0	0.0	0.0	6.397095819559937	0.0
44	0.0	0.0	0.0	6.558859968512798	0.0
45	0.0	0.0	0.0	6.743779654506403	0.0
46	0.0	0.0	0.0	6.946769315667318	0.0
47	0.0	0.0	0.0	7.124547574349291	0.0
48	0.0	0.0	0.0	7.314985635992794	0.0
49	0.0	0.0	0.0	7.522952655583027	0.0
50	0.0	0.0	0.0	7.7314606923938385	0.0
51	0.0	0.0	0.0	7.9307714364548225	0.0
52	0.0	0.0	0.0	8.13495133550101	0.0
53	0.0	0.0	0.0	8.362935992252634	0.0
54	0.0	0.0	0.0	8.591894480001299	0.0
55	0.0	0.0	0.0	8.827236970952786	0.0
56	0.0	0.0	0.0	9.066258379004204	0.0
57	0.0	0.0	0.0	9.304197752614465	0.0
58	0.0	0.0	0.0	9.52374254072507	0.0
59	0.0	0.0	0.0	9.741123259953364	0.0
60	0.0	0.0	0.0	9.974626292354886	0.0
61	0.0	0.0	0.0	10.198282811341885	0.0
62	0.0	0.0	0.0	10.451803480904797	0.0
63	1.082034441156262E-4	0.0	0.0	10.80552053971878	0.0
64	1.082034441156262E-4	0.0	0.0	11.110870659013077	0.0
65	1.082034441156262E-4	0.0	0.0	11.355518646158508	0.0
66	1.082034441156262E-4	0.0	0.0	11.58826425445122	0.0
67	1.082034441156262E-4	0.0	0.0	11.83366966570546	0.0
68	2.164068882312524E-4	0.0	0.0	12.097794272791702	0.0
69	2.164068882312524E-4	0.0	0.0	12.401737747312497	0.0
70	2.164068882312524E-4	0.0	0.0	12.65601584098422	0.0
71	2.164068882312524E-4	0.0	0.0	12.91375644486764	0.0
72	2.164068882312524E-4	0.0	0.0	13.179504103615619	0.0
73	2.164068882312524E-4	0.0	0.0	13.454124444781078	0.0
74	2.164068882312524E-4	0.0	0.0	13.756012053863675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACAC	20	4.0904753E-5	80.0	8
GGTCGGT	20	4.0904753E-5	80.0	9
ATTACGG	40	4.1836756E-11	80.0	2
TAGCGAG	45	1.8189894E-12	80.0	1
GCGTTCG	25	1.2846049E-6	80.0	80
CCGCTAG	40	4.1836756E-11	80.0	1
TAGCCCG	40	4.1836756E-11	80.0	1
CGTTAAG	20	4.0904753E-5	80.0	8
TACGACT	20	4.0904753E-5	80.0	6
ACTACGG	50	0.0	80.0	2
GTCGTAA	15	0.0013097094	80.0	8
TATTACG	40	4.1836756E-11	80.0	1
GAACGAT	20	4.0904753E-5	80.0	9
TGTAGCG	70	0.0	80.0	1
CGCGTGG	25	1.2846049E-6	80.0	2
CGTCAGG	60	0.0	80.0	2
ATCGCTG	45	1.8189894E-12	80.0	1
CGGGACT	150	0.0	77.33333	6
GGAGCGT	100	0.0	76.0	8
TACGGGA	255	0.0	75.29412	4
>>END_MODULE