FastQCFastQC Report
Thu 26 May 2016
SRR1547757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547757_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2942595
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169290.5753085286966096No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41650.1415417344214885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG700.080.01
CGTAAGG4850.073.402062
CGTTTTT85750.071.5568541
GTTAGCG3100.069.6774141
TTACGCG2550.067.450981
TAGGGAC30800.066.883125
CGGGGTA5100.066.6666646
TAGGGAG37650.066.50735
TAGCGGG20350.065.651113
CGAACGG1050.064.76192
ACGGGAC8850.064.6327745
TACGGGA10950.064.292244
ACGGGAT13700.064.2335745
CGAAACG4300.064.1860480
CGGGATC11050.064.0723956
TACGGGT2700.063.70374
GCGGGAT30850.063.4035645
GGGCGAT14550.063.230247
TTAGGGA42700.063.1381724
ACGTAAG2100.062.857141
Produced by FastQC (version 0.11.5)