FastQCFastQC Report
Thu 26 May 2016
SRR1547756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547756_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1454069
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75700.5206080316683734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG304.060894E-880.000011
TATAGCG750.074.6666641
TACGCGG700.074.285712
CGTTAGG3450.073.043482
GTCGACG550.072.727281
GTCGAAC403.7143764E-970.09
CGTTTTT38650.069.5472261
TAACGGG3650.069.041093
TTTGCGG4250.068.705882
CTATGCG700.068.571431
GCGTATG351.1817065E-768.571431
AGGGCGA4750.067.368426
CGAGGGA10100.066.534664
TAGGGAC16950.066.076695
CGTAAGG1950.065.641022
TAGGGCG2600.064.615395
GGCGATA1300.064.615398
ACGTTAG750.064.01
AGGGATT30900.063.948226
TAAGGGA17800.063.595514
Produced by FastQC (version 0.11.5)