FastQCFastQC Report
Thu 26 May 2016
SRR1547755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547755_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1423499
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84090.590727496120475No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21000.14752381280211646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG451.8189894E-1280.01
GCGTAAG1500.074.6666641
ACGGGAT7450.074.093965
ACGGGTA650.073.846155
ATATACG403.7143764E-970.01
CGTTTTT41700.068.489211
TATAGCG1000.068.01
ATAGGGA25300.066.719374
CGTAGCA303.796049E-666.6666780
CGTAAGG2400.066.666672
TAGGGAC16700.066.347315
CCGGGAT2800.065.714285
CGTAGGG6700.065.6716463
TACGGGA7400.065.40544
TAGGGAT30150.064.477615
CGGGATC6950.064.4604346
CGGGATT9600.064.166676
TAGGGAG19300.064.041455
GTAGGGA27550.063.4482734
GCGGGAT14200.063.098595
Produced by FastQC (version 0.11.5)