##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547755_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1423499
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76332403464983	31.0	31.0	33.0	30.0	34.0
2	32.2143570174619	33.0	31.0	34.0	31.0	34.0
3	32.36322399945487	34.0	31.0	34.0	30.0	34.0
4	35.968145393849944	37.0	35.0	37.0	35.0	37.0
5	35.90752013173174	37.0	35.0	37.0	35.0	37.0
6	35.970357548547625	37.0	35.0	37.0	35.0	37.0
7	36.222548804038496	37.0	35.0	37.0	35.0	37.0
8	36.187931287622966	37.0	35.0	37.0	35.0	37.0
9	38.07263510546899	39.0	39.0	39.0	37.0	39.0
10-11	37.50343695359112	39.0	37.5	39.0	35.0	39.0
12-13	36.54947632558927	39.0	35.0	39.0	33.0	39.0
14-15	37.505836674279365	40.0	35.0	41.0	33.0	41.0
16-17	37.75005567267697	40.0	35.0	41.0	33.0	41.0
18-19	37.65095058022521	39.0	35.5	41.0	33.0	41.0
20-21	37.447571441918825	39.0	35.0	41.0	33.0	41.0
22-23	37.258867761761685	39.0	35.0	41.0	33.0	41.0
24-25	37.18959444298872	39.0	35.0	41.0	33.0	41.0
26-27	37.1820155827296	39.0	35.0	41.0	33.0	41.0
28-29	37.19150733509472	39.0	35.0	41.0	33.0	41.0
30-31	37.03074923129556	39.0	35.0	41.0	32.5	41.0
32-33	36.7197058094175	39.0	35.0	41.0	31.5	41.0
34-35	36.43818330746983	38.5	35.0	41.0	31.0	41.0
36-37	36.22572794220439	38.0	35.0	41.0	30.0	41.0
38-39	36.14777671076692	38.0	35.0	41.0	30.0	41.0
40-41	36.00591851487075	38.0	35.0	41.0	30.0	41.0
42-43	35.9539704629227	38.0	35.0	41.0	30.0	41.0
44-45	35.8342085242069	38.0	35.0	40.0	30.0	41.0
46-47	35.76905603727154	38.0	35.0	40.0	30.0	41.0
48-49	35.684680143786544	37.5	35.0	40.0	29.0	41.0
50-51	35.50835617025372	37.0	35.0	40.0	29.0	41.0
52-53	35.340959143631295	37.0	34.5	40.0	29.0	41.0
54-55	35.1206116056281	36.0	34.0	40.0	28.0	41.0
56-57	34.80402163963585	36.0	34.0	40.0	27.5	41.0
58-59	34.54052584511827	36.0	34.0	39.0	26.5	41.0
60-61	34.238373542938916	35.0	34.0	39.0	26.0	41.0
62-63	33.859157610929124	35.0	33.5	39.0	26.0	40.0
64-65	33.56929404235619	35.0	33.0	38.0	25.0	40.0
66-67	33.3398010817008	35.0	33.0	37.5	25.0	40.0
68-69	32.99731050039375	35.0	33.0	37.0	24.5	39.5
70-71	32.794085910843634	35.0	33.0	36.0	24.0	39.0
72-73	32.56791504595367	35.0	33.0	36.0	24.0	39.0
74-75	32.26419091267363	35.0	32.5	35.5	23.5	38.0
76-77	30.176480629772133	33.0	29.5	34.5	19.5	36.0
78-79	31.30080175679786	34.0	31.0	35.0	20.5	36.5
80-81	31.627542766099587	34.5	32.0	35.0	22.0	36.0
82-83	31.519163694530167	35.0	32.0	35.0	20.0	36.0
84-85	31.358373627238237	34.0	32.0	35.0	20.0	36.0
86	30.946880187481693	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	14.0
11	17.0
12	35.0
13	86.0
14	238.0
15	656.0
16	1500.0
17	3080.0
18	6030.0
19	8786.0
20	11153.0
21	11697.0
22	10760.0
23	10332.0
24	11055.0
25	12541.0
26	14664.0
27	17759.0
28	21633.0
29	26561.0
30	32558.0
31	40173.0
32	50688.0
33	69095.0
34	180217.0
35	155824.0
36	120707.0
37	202865.0
38	274656.0
39	127583.0
40	522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.93483030195314	23.78399984826122	30.618778095383277	14.662391754402357
2	29.95681767250978	25.874342026232544	31.469779746947484	12.69906055431019
3	28.425450246189143	24.57908294982996	33.68973213188067	13.305734672100225
4	27.38063040437682	26.8469454492065	31.499495257811912	14.272928888604769
5	23.60015707773592	32.52583949830664	30.141924932859105	13.732078491098342
6	24.030364615640757	39.404453392661324	26.13082271220422	10.434359279493698
7	89.11913531375856	3.3578527276801737	6.09575419441812	1.427257764143143
8	90.17168259338433	2.508115565939983	5.651145522406408	1.6690563182692786
9	85.66616485153837	5.391714360178686	6.683109717674547	2.2590110706084094
10-11	42.62198287459282	27.29907081072765	19.060884482532124	11.018061832147406
12-13	31.686148005723926	24.673217192284643	27.183264617677988	16.45737018431344
14-15	15.324598050297189	32.27244978746033	35.39949799753986	17.003454164702607
16-17	21.51030664580727	25.224640129708558	33.09696740215483	20.168085822329346
18-19	24.586705013491404	27.01951318546764	30.219972054774892	18.173809746266066
20-21	27.3397101086829	26.582280704096036	29.1843197641867	16.89368942303437
22-23	21.61174682946739	23.595028869005176	31.889063497761498	22.904160803765933
24-25	19.18505035830724	26.62608122661133	35.97213626423341	18.216732150848017
26-27	17.645885244738494	34.06489923772338	29.9349349736108	18.354280543927324
28-29	16.465624492886892	27.496893218751822	37.185344000944156	18.852138287417134
30-31	22.70121018701102	28.07307908189609	30.75594011657191	18.469770614520982
32-33	26.964824000578858	27.462787118220668	27.598087529390607	17.97430135180987
34-35	20.22175639041545	27.418284101358694	30.37360756839309	21.986351939832762
36-37	24.047119105808996	27.4487021065698	30.382142874705213	18.12203591291599
38-39	20.060990559178475	30.48930838729075	28.30676382631811	21.142937227212663
40-41	21.464925511011952	26.350984440452713	28.92713658386834	23.256953464666992
42-43	21.68937245477517	24.594221703000844	28.91407018902015	24.80233565320383
44-45	19.78585864830253	30.264053575028854	29.150600035546216	20.7994877411224
46-47	21.396748434666975	29.932967989440108	27.616492881273537	21.053790694619384
48-49	21.168191898975692	26.239006841592445	32.40602908748092	20.186772171950945
50-51	19.60675771461729	30.813685151868743	29.15467450275694	20.42488263075703
52-53	23.306303692521034	25.363101765438543	30.196403369443885	21.134191172596537
54-55	23.6199674183122	26.26095978992609	30.173150806568884	19.945921985192825
56-57	23.04996350541869	24.183227385477615	29.548738706525256	23.218070402578437
58-59	24.509571134226295	25.907394385243688	28.52762804891328	21.055406431616742
60-61	25.289901854514824	28.13820030783302	27.125905954271833	19.445991883380316
62-63	23.89088436310809	25.049894660972715	30.524643852928595	20.5345771229906
64-65	20.456916372965487	29.004586585589454	30.780141046814926	19.758355994630133
66-67	19.33826437531744	27.352390131640412	27.348069791408353	25.96127570163379
68-69	18.988211442368417	29.97620651647806	26.098156725083754	24.93742531606977
70-71	19.66250064102609	28.89471646976921	29.80514914306227	21.63763374614243
72-73	23.786247830170588	28.91705578999353	27.10858946862625	20.188106911209633
74-75	23.01097506917813	30.748669300083808	24.502967687367537	21.737387943370525
76-77	26.820039915728778	26.766088349904006	26.679154674502758	19.734717059864458
78-79	20.04859153395963	28.286250991395146	30.894752999475237	20.770404475169986
80-81	17.662358737168063	27.6069038334414	31.534022854951075	23.19671457443946
82-83	19.74177712804856	26.59042963851748	31.283583620360815	22.38420961307314
84-85	17.490914991861604	26.166825547471404	30.135356610717672	26.206902849949316
86	18.930255658767585	26.137988154540327	31.45790759248865	23.47384859420344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	716.0
1	927.5
2	3265.0
3	3480.0
4	1620.5
5	1823.5
6	2187.0
7	2399.0
8	2453.0
9	2574.0
10	2677.0
11	2706.0
12	2741.5
13	2867.5
14	2951.0
15	2898.5
16	2884.0
17	2960.5
18	3385.5
19	3689.0
20	3858.5
21	4111.0
22	4319.5
23	4814.5
24	5981.5
25	7871.5
26	9820.5
27	11481.5
28	14592.0
29	16981.0
30	18315.5
31	21559.5
32	23315.0
33	24865.5
34	27313.5
35	31150.0
36	34243.0
37	37278.0
38	39863.5
39	42081.0
40	49703.5
41	58606.0
42	67598.0
43	72643.0
44	73905.0
45	74480.5
46	75955.5
47	83586.5
48	89304.5
49	80761.0
50	71969.0
51	62821.0
52	47675.0
53	41627.0
54	39384.0
55	37090.5
56	36201.5
57	35413.0
58	32084.0
59	24367.5
60	17357.5
61	14428.5
62	11546.5
63	7843.5
64	6716.0
65	6006.0
66	4322.5
67	3212.0
68	2899.5
69	2359.5
70	1670.5
71	1346.0
72	1090.5
73	646.0
74	362.0
75	206.5
76	131.5
77	88.0
78	50.5
79	42.0
80	34.5
81	29.5
82	22.5
83	17.0
84	14.0
85	9.0
86	11.0
87	10.5
88	7.0
89	3.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1423499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.60666590600946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.09297821324694	13.466839965106958
2	7.5640174709190395	2.5122622209354217
3	2.2816672125601145	1.1367265532304507
4	1.0833600629875249	0.7196399448778875
5	0.6410934868265395	0.5323212675123509
6	0.4756017366091788	0.47388954865119254
7	0.3186551150362416	0.3704259324253513
8	0.28672544082436335	0.380924288201879
9	0.22756395866538057	0.34011707704244293
>10	2.8398834323914026	11.558406047861144
>50	1.3626525542851131	16.522785856873284
>100	1.8185712615214438	50.51300308331088
>500	0.005954162222006599	0.6410876508662778
>1k	8.505946031437998E-4	0.23766221716040817
>5k	4.252973015718999E-4	0.5939083459440928
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8409	0.590727496120475	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2100	0.14752381280211646	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029645261429758644	0.0
2	0.0	0.0	0.0	0.09863020627341501	0.0
3	0.0	0.0	0.0	0.14443283767673878	0.0
4	0.0	0.0	0.0	0.22205846298451914	0.0
5	0.0	0.0	0.0	0.38988436240559354	0.0
6	0.0	0.0	0.0	0.5057256801725888	0.0
7	0.0	0.0	0.0	0.5942399678538587	0.0
8	0.0	0.0	0.0	0.766421332224329	0.0
9	0.0	0.0	0.0	0.8222696327851302	0.0
10	0.0	0.0	0.0	0.9438714041948747	0.0
11	0.0	0.0	0.0	1.1789962620275813	0.0
12	0.0	0.0	0.0	1.3658597582435956	0.0
13	0.0	0.0	0.0	1.427468512447146	0.0
14	0.0	0.0	0.0	1.4516343179728262	0.0
15	0.0	0.0	0.0	1.4925194889494127	0.0
16	0.0	0.0	0.0	1.5792775407639907	0.0
17	0.0	0.0	0.0	1.697507339309687	0.0
18	0.0	0.0	0.0	1.8338614919996432	0.0
19	0.0	0.0	0.0	1.9304544646676955	0.0
20	0.0	0.0	0.0	2.041518820877289	0.0
21	0.0	0.0	0.0	2.1685297987564445	0.0
22	0.0	0.0	0.0	2.3097311624384704	0.0
23	0.0	0.0	0.0	2.4493167891231393	0.0
24	0.0	0.0	0.0	2.5563066781220076	0.0
25	0.0	0.0	0.0	2.6472094465819787	0.0
26	0.0	0.0	0.0	2.732632759137871	0.0
27	0.0	0.0	0.0	2.834915936014005	0.0
28	0.0	0.0	0.0	2.933054396244746	0.0
29	0.0	0.0	0.0	3.058238888822542	0.0
30	0.0	0.0	0.0	3.2179158538221664	0.0
31	0.0	0.0	0.0	3.3410631127946	0.0
32	0.0	0.0	0.0	3.445453772710764	0.0
33	0.0	0.0	0.0	3.5526544100136355	0.0
34	0.0	0.0	0.0	3.680719129412806	0.0
35	0.0	0.0	0.0	3.8281024433455872	0.0
36	0.0	0.0	0.0	3.954832423486072	0.0
37	0.0	0.0	0.0	4.085496371967946	0.0
38	0.0	0.0	0.0	4.233863177986075	0.0
39	0.0	0.0	0.0	4.519567628779507	0.0
40	0.0	0.0	0.0	4.668988176317651	0.0
41	0.0	0.0	0.0	4.811454029823695	0.0
42	0.0	0.0	0.0	4.965511040049905	0.0
43	0.0	0.0	0.0	5.117882063844092	0.0
44	0.0	0.0	0.0	5.268286103467583	0.0
45	0.0	0.0	0.0	5.407239485240242	0.0
46	0.0	0.0	0.0	5.554341801434353	0.0
47	0.0	0.0	0.0	5.7211842087700795	0.0
48	0.0	0.0	0.0	5.883460402852408	0.0
49	0.0	0.0	0.0	6.048125077713437	0.0
50	0.0	0.0	0.0	6.223467666643953	0.0
51	0.0	0.0	0.0	6.398669756705133	0.0
52	0.0	0.0	0.0	6.622484455556344	0.0
53	0.0	0.0	0.0	6.801901511697585	0.0
54	0.0	0.0	0.0	6.986095529396227	0.0
55	0.0	0.0	0.0	7.170289547094869	0.0
56	0.0	0.0	0.0	7.368182204553709	0.0
57	0.0	0.0	0.0	7.5490744988229705	0.0
58	0.0	0.0	0.0	7.720764117150767	0.0
59	0.0	0.0	0.0	7.902639903505377	0.0
60	0.0	0.0	0.0	8.14176897911414	0.0
61	0.0	0.0	0.0	8.373802861821469	0.0
62	0.0	0.0	0.0	8.626349579451759	0.0
63	0.0	0.0	0.0	8.889012215674194	0.0
64	0.0	0.0	0.0	9.123434579160223	0.0
65	0.0	0.0	0.0	9.342542565888701	0.0
66	7.024943466767452E-5	0.0	0.0	9.553361119326393	0.0
67	1.4049886933534904E-4	0.0	0.0	9.765022665980096	0.0
68	1.4049886933534904E-4	0.0	0.0	10.02677205955185	0.0
69	1.4049886933534904E-4	0.0	0.0	10.44876041360057	0.0
70	2.1074830400302354E-4	0.0	0.0	10.6738396022758	0.0
71	2.809977386706981E-4	0.0	0.0	10.904187498551105	0.0
72	2.809977386706981E-4	0.0	0.0	11.139523104687814	0.0
73	3.512471733383726E-4	0.0	0.0	11.365796533752395	0.0
74	3.512471733383726E-4	0.0	0.0	11.61454978191063	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	45	1.8189894E-12	80.0	1
GCGTAAG	150	0.0	74.666664	1
ACGGGAT	745	0.0	74.09396	5
ACGGGTA	65	0.0	73.84615	5
ATATACG	40	3.7143764E-9	70.0	1
CGTTTTT	4170	0.0	68.48921	1
TATAGCG	100	0.0	68.0	1
ATAGGGA	2530	0.0	66.71937	4
CGTAGCA	30	3.796049E-6	66.66667	80
CGTAAGG	240	0.0	66.66667	2
TAGGGAC	1670	0.0	66.34731	5
CCGGGAT	280	0.0	65.71428	5
CGTAGGG	670	0.0	65.671646	3
TACGGGA	740	0.0	65.4054	4
TAGGGAT	3015	0.0	64.47761	5
CGGGATC	695	0.0	64.460434	6
CGGGATT	960	0.0	64.16667	6
TAGGGAG	1930	0.0	64.04145	5
GTAGGGA	2755	0.0	63.448273	4
GCGGGAT	1420	0.0	63.09859	5
>>END_MODULE