FastQCFastQC Report
Thu 26 May 2016
SRR1547754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547754_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3058745
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204580.6688364018576246No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46990.15362509787510892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG1100.072.727272
CGAGGGA17350.072.1613854
TACGCGG1450.071.7241442
CGAACGG950.071.578952
GCGAACG403.7161954E-970.01
TCGATAG1850.069.1891861
CGTTTTT113500.069.110131
AGTACGG2550.069.019612
TACGGGA10450.068.133974
GTTAGCG1300.067.692311
GGGCGAT19700.067.411177
GCGATAT3250.065.230779
CGATAGG5400.064.444442
TAGGGCG4200.063.809525
TAGGGAC20150.063.325065
AGGGCGA10200.063.1372576
TAGCGGG15100.062.2516563
GGCGATT6000.060.6666688
TCTAGCG2250.060.4444431
CTTGCGG4500.060.4444432
Produced by FastQC (version 0.11.5)