##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547754_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3058745
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.854208507083786	31.0	31.0	33.0	30.0	34.0
2	32.305167642284665	33.0	31.0	34.0	31.0	34.0
3	32.44906685585101	34.0	31.0	34.0	31.0	34.0
4	36.036651960199364	37.0	35.0	37.0	35.0	37.0
5	35.989415266718865	37.0	35.0	37.0	35.0	37.0
6	36.05184315789646	37.0	35.0	37.0	35.0	37.0
7	36.27243493655077	37.0	35.0	37.0	35.0	37.0
8	36.22363060667038	37.0	36.0	37.0	35.0	37.0
9	38.09008662049305	39.0	39.0	39.0	37.0	39.0
10-11	37.60224945198112	39.0	37.5	39.0	35.0	39.0
12-13	36.86719438854824	39.0	35.0	39.0	33.5	39.0
14-15	37.945194189120045	40.0	35.0	41.0	33.0	41.0
16-17	38.09547772043763	40.0	35.0	41.0	34.0	41.0
18-19	37.96333152976139	40.0	36.0	41.0	34.0	41.0
20-21	37.762426910383184	40.0	35.0	41.0	34.0	41.0
22-23	37.57767875386801	39.0	35.0	41.0	33.0	41.0
24-25	37.491335662175175	39.0	35.0	41.0	33.0	41.0
26-27	37.4651281816562	39.0	35.0	41.0	33.0	41.0
28-29	37.45008393965499	39.0	35.0	41.0	33.0	41.0
30-31	37.28518215804195	39.0	35.0	41.0	33.0	41.0
32-33	36.95355513454048	39.0	35.0	41.0	32.5	41.0
34-35	36.64842737789518	39.0	35.0	41.0	31.5	41.0
36-37	36.43950443727738	39.0	35.0	41.0	31.0	41.0
38-39	36.34990854092119	39.0	35.0	41.0	31.0	41.0
40-41	36.19539418944698	38.5	35.0	41.0	30.0	41.0
42-43	36.14625426441236	38.5	35.0	41.0	30.0	41.0
44-45	36.03796982095598	38.0	35.0	41.0	30.0	41.0
46-47	35.937610686735894	38.0	35.0	41.0	30.0	41.0
48-49	35.82720020792841	38.0	35.0	40.0	30.0	41.0
50-51	35.64787617143632	38.0	35.0	40.0	29.0	41.0
52-53	35.45222207147049	37.5	35.0	40.0	29.0	41.0
54-55	35.28992527981247	37.0	35.0	40.0	28.5	41.0
56-57	35.027138090949066	36.5	34.5	40.0	27.5	41.0
58-59	34.727714634596865	36.0	34.0	40.0	27.0	41.0
60-61	34.40526637558868	36.0	34.0	39.0	26.0	41.0
62-63	34.02977789910568	35.0	33.5	39.0	26.0	41.0
64-65	33.72528389911548	35.0	33.0	39.0	25.0	40.0
66-67	33.437959686080404	35.0	33.0	38.0	25.0	40.0
68-69	33.0238568432478	35.0	33.0	37.0	23.0	40.0
70-71	32.73065799862362	35.0	33.0	37.0	23.0	39.0
72-73	32.459592087604555	35.0	33.0	36.0	22.0	39.0
74-75	32.158969119687974	35.0	32.5	36.0	20.5	38.5
76-77	30.10039297162725	33.0	29.5	34.5	18.5	36.0
78-79	31.187736473619083	34.0	31.0	35.0	19.0	37.0
80-81	31.389585598014875	35.0	32.0	35.0	19.0	36.5
82-83	31.200911158007614	35.0	32.0	35.0	18.0	36.0
84-85	30.99502426648838	35.0	32.0	35.0	17.5	36.0
86	30.566983190818455	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	17.0
10	33.0
11	56.0
12	84.0
13	166.0
14	469.0
15	1336.0
16	3478.0
17	8039.0
18	17033.0
19	24979.0
20	28217.0
21	26931.0
22	23018.0
23	20082.0
24	20212.0
25	22649.0
26	26554.0
27	32906.0
28	41152.0
29	51689.0
30	65301.0
31	82538.0
32	103827.0
33	140591.0
34	348399.0
35	325913.0
36	260621.0
37	441691.0
38	623418.0
39	316044.0
40	1290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.808862785227277	22.459145826147655	30.924088147263014	14.80790324136206
2	31.82860944603097	25.214164632880482	29.723726561056907	13.233499360031647
3	28.87390089726342	24.24425704006055	33.01530529678022	13.866536765895818
4	26.39170640246245	26.410047257944026	32.48172044416909	14.716525895424432
5	23.693639057848888	31.199266365780737	31.312319268196596	13.794775308173776
6	22.2750180220973	39.33276556234665	27.913049306169686	10.479167109386365
7	85.77367515108321	4.591360182035443	7.681418359490576	1.9535463073907762
8	86.38340234311784	4.11521718874898	7.463028137356988	2.0383523307761844
9	81.98104124403963	6.317721810742641	8.790533372347156	2.9107035728705726
10-11	40.00457704058364	26.24842868562106	21.211101285004144	12.535892988791156
12-13	29.161715017106687	26.783272224392686	27.78816148453042	16.266851273970207
14-15	16.794910984733935	32.123452592484824	35.265672686019926	15.815963736761319
16-17	20.375202901843732	25.35412399529873	33.71498768285686	20.555685420000685
18-19	23.56762332263723	28.03903234823433	30.633691268804693	17.75965306032376
20-21	25.20515767087482	28.075092889403987	29.878937276562773	16.840812163158418
22-23	21.05219624388434	26.020230519379677	30.82228168742409	22.105291549311893
24-25	19.405802052802702	27.152116309139863	34.42872812215467	19.013353515902764
26-27	17.752869232315867	34.03467762105046	29.945696682789837	18.26675646384383
28-29	16.75327626199634	29.04084845255162	35.79935561807211	18.40651966737992
30-31	21.227431511943625	29.681356242511225	31.168077103517945	17.923135142027206
32-33	24.016712736759686	29.127942996228846	29.25049325785575	17.604851009155716
34-35	19.441584702222645	30.173747729869604	29.965214491564353	20.4194530763434
36-37	22.60523515363327	30.03387010031892	29.438805784725435	17.922088961322373
38-39	19.688695854018558	31.388837578810918	28.82582562456171	20.096640942608815
40-41	20.504324486022863	28.278951007684523	29.270566850129708	21.946157656162903
42-43	20.79584927805358	27.600470127454237	29.235290944488675	22.368389650003515
44-45	19.24032568913067	32.26305233028579	28.19479884723964	20.301823133343905
46-47	20.908330050396486	31.73657823715282	27.923805351541237	19.431286360909457
48-49	21.24153860488534	27.679048106331187	30.79622524924438	20.28318803953909
50-51	19.0386907048479	31.846361824866083	28.25516674322312	20.859780727062898
52-53	21.608143209061232	27.642709673411808	30.185778807975165	20.563368309551795
54-55	20.735563114937662	28.428816393651644	31.06350807275533	19.772112418655365
56-57	21.740664880531067	26.965634598503634	29.6298645359453	21.66383598502
58-59	22.247212500551697	28.68777881124448	28.533172918958595	20.53183576924523
60-61	22.79928532780601	30.286375621374123	27.59635079092896	19.317988259890903
62-63	22.608700627218028	27.304891385192292	30.792923241394753	19.293484746194927
64-65	19.147477151576872	31.559479459713053	29.824519533338016	19.468523855372055
66-67	18.767631822855453	30.207356285012317	26.595842412492704	24.429169479639526
68-69	17.996482217380002	32.65953847084344	26.094198764525974	23.249780547250587
70-71	19.388000634247053	30.024209275372744	29.521928111039003	21.0658619793412
72-73	21.773962850777036	30.188443299457784	27.63711914527037	20.400474704494815
74-75	20.08006551706664	32.19948050589376	26.243246821817444	21.477207155222157
76-77	24.00456723263953	29.147656963885517	27.06892859653224	19.778847206942718
78-79	19.239851638498795	30.516143058672757	29.529774466325243	20.714230836503205
80-81	17.196562642521688	29.588671170692553	30.749555781864785	22.465210404920974
82-83	18.653696205469892	28.744926432245904	30.94439059156615	21.656986770718056
84-85	17.07569607796662	28.298991906811455	30.44812496628519	24.177187048936737
86	18.283544394841673	28.215558995601135	31.095367544532156	22.405529065025036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2004.0
1	2639.0
2	8080.5
3	8292.0
4	4252.0
5	5777.5
6	7574.5
7	8401.0
8	9062.5
9	9083.5
10	8195.0
11	7661.0
12	7407.0
13	7598.0
14	7757.0
15	8332.5
16	8853.0
17	8596.5
18	9277.0
19	11088.0
20	12074.5
21	12132.0
22	13437.0
23	13975.0
24	15763.0
25	19210.0
26	23792.5
27	29584.0
28	35074.5
29	38464.0
30	42476.5
31	54107.0
32	61667.0
33	63600.5
34	69689.0
35	78794.0
36	83802.0
37	84610.5
38	89041.0
39	100465.0
40	117502.0
41	138210.0
42	156396.0
43	163109.0
44	171584.5
45	175251.5
46	172901.0
47	175257.0
48	170988.0
49	148399.0
50	129977.0
51	113722.5
52	95382.0
53	85179.5
54	72761.0
55	65345.0
56	61898.0
57	61565.0
58	52921.5
59	38077.5
60	27448.0
61	21741.0
62	19293.5
63	15141.5
64	12159.0
65	9016.5
66	4547.5
67	3097.0
68	2513.5
69	1636.0
70	862.0
71	506.0
72	472.5
73	380.0
74	217.5
75	95.0
76	86.5
77	91.0
78	61.0
79	37.0
80	34.5
81	26.5
82	18.5
83	16.5
84	12.5
85	6.0
86	4.0
87	4.0
88	3.0
89	2.0
90	3.0
91	2.0
92	1.0
93	2.0
94	2.0
95	1.5
96	0.5
97	1.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3058745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.400149724342626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42034020953663	12.861053208702872
2	9.476127113385825	3.1081980693286044
3	3.132536051650913	1.5412218079192819
4	1.4598510946495062	0.9576710610998953
5	0.8520230355923993	0.6986652676152127
6	0.5930766190272467	0.5835927210032255
7	0.4447931412329907	0.5106271878807207
8	0.3635470354098751	0.4769780650050274
9	0.3023219378674592	0.4462312541381766
>10	2.5981654563456402	9.319765607717967
>50	0.7876507972521102	9.399297099764896
>100	1.5379546682597582	55.80336035602467
>500	0.02839114911042169	2.7837417429983704
>1k	0.003020335011746988	0.8340195670261079
>5k	0.0	0.0
>10k+	2.0135566744979922E-4	0.6755769837749491
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20458	0.6688364018576246	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4699	0.15362509787510892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.538629405197229E-5	0.0	0.0	0.041193365252742545	0.0
2	6.538629405197229E-5	0.0	0.0	0.1600329546922022	0.0
3	6.538629405197229E-5	0.0	0.0	0.23565220376330814	0.0
4	6.538629405197229E-5	0.0	0.0	0.34229724936207495	0.0
5	6.538629405197229E-5	0.0	0.0	0.5789302475361627	0.0
6	6.538629405197229E-5	0.0	0.0	0.7688774317571422	0.0
7	6.538629405197229E-5	0.0	0.0	0.8999442581843207	0.0
8	6.538629405197229E-5	0.0	0.0	1.14707175655375	0.0
9	6.538629405197229E-5	0.0	0.0	1.2346566974363669	0.0
10	6.538629405197229E-5	0.0	0.0	1.4166594469300318	0.0
11	6.538629405197229E-5	0.0	0.0	1.7543469625614427	0.0
12	6.538629405197229E-5	0.0	0.0	1.9914703579409203	0.0
13	6.538629405197229E-5	0.0	0.0	2.0797745480581087	0.0
14	6.538629405197229E-5	0.0	0.0	2.1154100783164336	0.0
15	6.538629405197229E-5	0.0	0.0	2.1754673894031704	0.0
16	6.538629405197229E-5	0.0	0.0	2.3146094231457672	0.0
17	6.538629405197229E-5	0.0	0.0	2.494487118082743	0.0
18	6.538629405197229E-5	0.0	0.0	2.698361582936793	0.0
19	6.538629405197229E-5	0.0	0.0	2.8353131758286487	0.0
20	6.538629405197229E-5	0.0	0.0	2.971349360603777	0.0
21	6.538629405197229E-5	0.0	0.0	3.1654812676440827	0.0
22	6.538629405197229E-5	0.0	0.0	3.3701076748797303	0.0
23	6.538629405197229E-5	0.0	0.0	3.5918325980099683	0.0
24	6.538629405197229E-5	0.0	0.0	3.751080917173547	0.0
25	6.538629405197229E-5	0.0	0.0	3.897219284379705	0.0
26	6.538629405197229E-5	0.0	0.0	4.035707455181782	0.0
27	6.538629405197229E-5	0.0	0.0	4.1814208114766025	0.0
28	6.538629405197229E-5	0.0	0.0	4.339655643082375	0.0
29	6.538629405197229E-5	0.0	0.0	4.513092788055232	0.0
30	6.538629405197229E-5	0.0	0.0	4.691662757111168	0.0
31	6.538629405197229E-5	0.0	0.0	4.880596453774342	0.0
32	6.538629405197229E-5	0.0	0.0	5.047462276194976	0.0
33	6.538629405197229E-5	0.0	0.0	5.225084143987158	0.0
34	6.538629405197229E-5	0.0	0.0	5.39570967831578	0.0
35	6.538629405197229E-5	0.0	0.0	5.6009899484919465	0.0
36	6.538629405197229E-5	0.0	0.0	5.780213780488403	0.0
37	6.538629405197229E-5	0.0	0.0	5.982780519461413	0.0
38	6.538629405197229E-5	0.0	0.0	6.194337873866569	0.0
39	6.538629405197229E-5	0.0	0.0	6.446565503172053	0.0
40	6.538629405197229E-5	0.0	0.0	6.665511508805082	0.0
41	6.538629405197229E-5	0.0	0.0	6.890799984961152	0.0
42	6.538629405197229E-5	0.0	0.0	7.117232721263132	0.0
43	6.538629405197229E-5	0.0	0.0	7.328528530492081	0.0
44	6.538629405197229E-5	0.0	0.0	7.533808800668248	0.0
45	6.538629405197229E-5	0.0	0.0	7.754651008828785	0.0
46	6.538629405197229E-5	0.0	0.0	7.989714735945625	0.0
47	6.538629405197229E-5	0.0	0.0	8.231742103379	0.0
48	6.538629405197229E-5	0.0	0.0	8.471023246462194	0.0
49	9.807944107795844E-5	0.0	0.0	8.727500984881054	0.0
50	9.807944107795844E-5	0.0	0.0	8.989863489764593	0.0
51	9.807944107795844E-5	0.0	0.0	9.256345331173407	0.0
52	9.807944107795844E-5	0.0	0.0	9.518119359410477	0.0
53	9.807944107795844E-5	0.0	0.0	9.774531711535287	0.0
54	9.807944107795844E-5	0.0	0.0	10.038855805240384	0.0
55	9.807944107795844E-5	0.0	0.0	10.295235464218168	0.0
56	9.807944107795844E-5	0.0	0.0	10.549522761786289	0.0
57	9.807944107795844E-5	0.0	0.0	10.815546899136738	0.0
58	9.807944107795844E-5	0.0	0.0	11.067741835295195	0.0
59	1.3077258810394459E-4	0.0	0.0	11.343639303047492	0.0
60	1.3077258810394459E-4	0.0	0.0	11.639512283632666	0.0
61	1.3077258810394459E-4	0.0	0.0	11.927538908931604	0.0
62	1.3077258810394459E-4	0.0	0.0	12.238973827501148	0.0
63	1.3077258810394459E-4	0.0	0.0	12.558418567092058	0.0
64	1.3077258810394459E-4	0.0	0.0	12.869493861044317	0.0
65	1.3077258810394459E-4	0.0	0.0	13.167949600244544	0.0
66	1.3077258810394459E-4	0.0	0.0	13.443912454290894	0.0
67	1.3077258810394459E-4	0.0	0.0	13.728964003210468	0.0
68	1.3077258810394459E-4	0.0	0.0	14.029773648996565	0.0
69	1.3077258810394459E-4	0.0	0.0	14.411400754230902	0.0
70	1.3077258810394459E-4	0.0	0.0	14.710346890636519	0.0
71	1.3077258810394459E-4	0.0	0.0	15.024070329497881	0.0
72	1.6346573512993073E-4	0.0	0.0	15.331778229306464	0.0
73	1.6346573512993073E-4	0.0	0.0	15.648051733635853	0.0
74	1.6346573512993073E-4	0.0	0.0	15.981750685330095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGG	110	0.0	72.72727	2
CGAGGGA	1735	0.0	72.161385	4
TACGCGG	145	0.0	71.724144	2
CGAACGG	95	0.0	71.57895	2
GCGAACG	40	3.7161954E-9	70.0	1
TCGATAG	185	0.0	69.189186	1
CGTTTTT	11350	0.0	69.11013	1
AGTACGG	255	0.0	69.01961	2
TACGGGA	1045	0.0	68.13397	4
GTTAGCG	130	0.0	67.69231	1
GGGCGAT	1970	0.0	67.41117	7
GCGATAT	325	0.0	65.23077	9
CGATAGG	540	0.0	64.44444	2
TAGGGCG	420	0.0	63.80952	5
TAGGGAC	2015	0.0	63.32506	5
AGGGCGA	1020	0.0	63.137257	6
TAGCGGG	1510	0.0	62.251656	3
GGCGATT	600	0.0	60.666668	8
TCTAGCG	225	0.0	60.444443	1
CTTGCGG	450	0.0	60.444443	2
>>END_MODULE