FastQCFastQC Report
Thu 26 May 2016
SRR1547753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547753_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3097161
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198330.6403606399538158No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39590.1278267419743436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33750.1089707638705253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1500.074.6666641
TCGTTAG1100.072.727271
CGTTTTT94350.070.673021
TAGGGCG6900.069.5652165
CGATAGG7400.067.5675662
ACGGGAC10400.067.307695
TAGGGAC29050.066.506035
CGGGATA7250.066.2068946
CGTTAGG5450.066.0550462
TGCGTAG1500.064.01
TAAGGGA38600.063.9378284
ACGGGAT12000.063.6666685
TACGGGA13600.063.529414
CTACGGG10650.062.347423
GCGATAG4150.061.6867451
GGTCGAC2600.061.5384648
ATTGCGG11700.060.8547022
TATGGGA31850.060.784934
TAGGGAG29450.060.7130745
GGGCGAT17100.060.5847937
Produced by FastQC (version 0.11.5)